diff --git a/browser/brave_profile_prefs.cc b/browser/brave_profile_prefs.cc
index 926d3f0d996a..59f9e1c7d09e 100644
--- a/browser/brave_profile_prefs.cc
+++ b/browser/brave_profile_prefs.cc
@@ -306,6 +306,8 @@ void RegisterProfilePrefs(user_prefs::PrefRegistrySyncable* registry) {
   // Disable Chromium's privacy sandbox
   registry->SetDefaultPrefValue(prefs::kPrivacySandboxApisEnabled,
                                 base::Value(false));
+  registry->SetDefaultPrefValue(prefs::kPrivacySandboxApisEnabledV2,
+                                base::Value(false));
 
   // Disable Chromium's privacy sandbox
   registry->SetDefaultPrefValue(prefs::kPrivacySandboxFlocEnabled,
diff --git a/chromium_src/chrome/browser/privacy_sandbox/privacy_sandbox_service_unittest.cc b/chromium_src/chrome/browser/privacy_sandbox/privacy_sandbox_service_unittest.cc
index b26dc794fde9..3cc4eeb8867f 100644
--- a/chromium_src/chrome/browser/privacy_sandbox/privacy_sandbox_service_unittest.cc
+++ b/chromium_src/chrome/browser/privacy_sandbox/privacy_sandbox_service_unittest.cc
@@ -153,3 +153,43 @@ TEST_F(PrivacySandboxServiceTest, OnPrivacySandboxPrefChanged) {
       prefs::kPrivacySandboxApisEnabled, true);
   testing::Mock::VerifyAndClearExpectations(&mock_privacy_sandbox_observer);
 }
+
+TEST_F(PrivacySandboxServiceTest, OnPrivacySandboxPrefV2Changed) {
+  // When either the main Privacy Sandbox pref, or the FLoC pref, are changed
+  // the FLoC ID should be reset. This will be propagated to the settings
+  // instance, which should then notify observers.
+  privacy_sandbox_test_util::MockPrivacySandboxObserver
+      mock_privacy_sandbox_observer;
+  PrivacySandboxSettingsFactory::GetForProfile(profile())->AddObserver(
+      &mock_privacy_sandbox_observer);
+  EXPECT_CALL(mock_privacy_sandbox_observer,
+              OnFlocDataAccessibleSinceUpdated(/*reset_compute_timer=*/true));
+
+  profile()->GetTestingPrefService()->SetBoolean(
+      prefs::kPrivacySandboxApisEnabledV2, false);
+  testing::Mock::VerifyAndClearExpectations(&mock_privacy_sandbox_observer);
+
+  EXPECT_CALL(mock_privacy_sandbox_observer,
+              OnFlocDataAccessibleSinceUpdated(/*reset_compute_timer=*/true));
+  profile()->GetTestingPrefService()->SetBoolean(
+      prefs::kPrivacySandboxFlocEnabled, false);
+  testing::Mock::VerifyAndClearExpectations(&mock_privacy_sandbox_observer);
+
+  // OnFlocDataAccessibleSinceUpdated() will be called twice because the attempt
+  // to enable the pref will be immediately followed by setting it to false.
+  EXPECT_CALL(mock_privacy_sandbox_observer,
+              OnFlocDataAccessibleSinceUpdated(/*reset_compute_timer=*/true))
+      .Times(2);
+  profile()->GetTestingPrefService()->SetBoolean(
+      prefs::kPrivacySandboxFlocEnabled, true);
+  testing::Mock::VerifyAndClearExpectations(&mock_privacy_sandbox_observer);
+
+  // OnFlocDataAccessibleSinceUpdated() will be called twice because the attempt
+  // to enable the pref will be immediately followed by setting it to false.
+  EXPECT_CALL(mock_privacy_sandbox_observer,
+              OnFlocDataAccessibleSinceUpdated(/*reset_compute_timer=*/true))
+      .Times(2);
+  profile()->GetTestingPrefService()->SetBoolean(
+      prefs::kPrivacySandboxApisEnabledV2, true);
+  testing::Mock::VerifyAndClearExpectations(&mock_privacy_sandbox_observer);
+}
diff --git a/chromium_src/components/privacy_sandbox/privacy_sandbox_settings_unittest.cc b/chromium_src/components/privacy_sandbox/privacy_sandbox_settings_unittest.cc
index 07899390b8d7..53b5e11507a8 100644
--- a/chromium_src/components/privacy_sandbox/privacy_sandbox_settings_unittest.cc
+++ b/chromium_src/components/privacy_sandbox/privacy_sandbox_settings_unittest.cc
@@ -479,9 +479,11 @@ TEST_F(PrivacySandboxSettingsTest, IsPrivacySandboxEnabled) {
   EXPECT_FALSE(privacy_sandbox_settings()->IsPrivacySandboxEnabled());
 
   // Check that even bypassing PrivacySandboxSettings::SetPrivacySandboxEnabled,
-  // and manually updating the preference, we still don't get this enabled.
+  // and manually updating the preferences, we still don't get this enabled.
   profile()->GetTestingPrefService()->SetBoolean(
       prefs::kPrivacySandboxApisEnabled, true);
+  profile()->GetTestingPrefService()->SetBoolean(
+      prefs::kPrivacySandboxApisEnabledV2, true);
   EXPECT_FALSE(privacy_sandbox_settings()->IsPrivacySandboxEnabled());
 }
 
@@ -503,6 +505,8 @@ TEST_F(PrivacySandboxSettingsTest, IsFlocAllowed) {
   // and manually updating the preferences, we still don't get this enabled.
   profile()->GetTestingPrefService()->SetBoolean(
       prefs::kPrivacySandboxApisEnabled, true);
+  profile()->GetTestingPrefService()->SetBoolean(
+      prefs::kPrivacySandboxApisEnabledV2, true);
   profile()->GetTestingPrefService()->SetBoolean(
       prefs::kPrivacySandboxFlocEnabled, true);
   EXPECT_FALSE(privacy_sandbox_settings()->IsFlocAllowed());
diff --git a/components/privacy_sandbox/brave_privacy_sandbox_settings.cc b/components/privacy_sandbox/brave_privacy_sandbox_settings.cc
index 5e4337710f97..c9c3e2b2afaf 100644
--- a/components/privacy_sandbox/brave_privacy_sandbox_settings.cc
+++ b/components/privacy_sandbox/brave_privacy_sandbox_settings.cc
@@ -27,6 +27,11 @@ BravePrivacySandboxSettings::BravePrivacySandboxSettings(
       base::BindRepeating(
           &BravePrivacySandboxSettings::OnPrivacySandboxPrefChanged,
           base::Unretained(this)));
+  user_prefs_registrar_.Add(
+      prefs::kPrivacySandboxApisEnabledV2,
+      base::BindRepeating(
+          &BravePrivacySandboxSettings::OnPrivacySandboxPrefChanged,
+          base::Unretained(this)));
   user_prefs_registrar_.Add(
       prefs::kPrivacySandboxFlocEnabled,
       base::BindRepeating(
@@ -38,10 +43,13 @@ BravePrivacySandboxSettings::~BravePrivacySandboxSettings() = default;
 
 void BravePrivacySandboxSettings::OnPrivacySandboxPrefChanged() {
   // Make sure that Private Sandbox features remain disabled even if we manually
-  // access the Pref service and try to change the preference from there.
+  // access the Pref service and try to change the preferences from there.
   if (pref_service_->GetBoolean(prefs::kPrivacySandboxApisEnabled)) {
     pref_service_->SetBoolean(prefs::kPrivacySandboxApisEnabled, false);
   }
+  if (pref_service_->GetBoolean(prefs::kPrivacySandboxApisEnabledV2)) {
+    pref_service_->SetBoolean(prefs::kPrivacySandboxApisEnabledV2, false);
+  }
   if (pref_service_->GetBoolean(prefs::kPrivacySandboxFlocEnabled)) {
     pref_service_->SetBoolean(prefs::kPrivacySandboxFlocEnabled, false);
   }