diff --git a/.github/workflows/test_and_deploy.yml b/.github/workflows/test_and_deploy.yml
index 98b39e0..d81aef4 100644
--- a/.github/workflows/test_and_deploy.yml
+++ b/.github/workflows/test_and_deploy.yml
@@ -36,16 +36,16 @@ jobs:
       fail-fast: false
       matrix:
         # Run tests on ubuntu across all supported versions
-        python-version: ["3.9", "3.10"]
+        python-version: ["3.10", "3.11", "3.12"]
         os: [ubuntu-latest]
         # Include a Windows test and old/new Mac runs
         include:
           - os: macos-13
-            python-version: "3.10"
+            python-version: "3.12"
           - os: macos-latest
-            python-version: "3.10"
+            python-version: "3.12"
           - os: windows-latest
-            python-version: "3.10"
+            python-version: "3.12"
     steps:
       # Cache atlases
       - name: Cache brainglobe directory
@@ -87,7 +87,7 @@ jobs:
 
     strategy:
       matrix:
-        python-version: ["3.10"]
+        python-version: ["3.12"]
 
     steps:
       - name: Checkout brainglobe-workflows repository
diff --git a/benchmarks/asv.latest-github.conf.json b/benchmarks/asv.latest-github.conf.json
index 6f9ce91..85e700c 100644
--- a/benchmarks/asv.latest-github.conf.json
+++ b/benchmarks/asv.latest-github.conf.json
@@ -64,7 +64,7 @@
     // The Pythons you'd like to test against.  If not provided, defaults
     // to the current version of Python used to run `asv`.
     "pythons": [
-        "3.10"
+        "3.12"
     ],
     // The list of conda channel names to be searched for benchmark
     // dependency packages in the specified order
diff --git a/benchmarks/asv.pip.conf.json b/benchmarks/asv.pip.conf.json
index f23943e..ab7dbbc 100644
--- a/benchmarks/asv.pip.conf.json
+++ b/benchmarks/asv.pip.conf.json
@@ -62,7 +62,7 @@
     // The Pythons you'd like to test against.  If not provided, defaults
     // to the current version of Python used to run `asv`.
     "pythons": [
-        "3.10"
+        "3.12"
     ],
     // The list of conda channel names to be searched for benchmark
     // dependency packages in the specified order
diff --git a/pyproject.toml b/pyproject.toml
index e91ef78..483c6c9 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -9,7 +9,7 @@ authors = [
 description = "A collection of end-to-end data analysis workflows executed using BrainGlobe tools."
 readme = "README.md"
 license = { file = "LICENSE" }
-requires-python = ">=3.9"
+requires-python = ">=3.10"
 dynamic = ["version"]
 
 classifiers = [
@@ -19,7 +19,8 @@ classifiers = [
     "License :: OSI Approved :: BSD License",
     "Operating System :: OS Independent",
     "Programming Language :: Python :: 3.10",
-    "Programming Language :: Python :: 3.9",
+    "Programming Language :: Python :: 3.11",
+    "Programming Language :: Python :: 3.12",
     "Programming Language :: Python",
     "Topic :: Scientific/Engineering :: Image Recognition",
 ]
@@ -84,17 +85,11 @@ include = ["brainglobe_workflows"]
 exclude = ["tests", "resources", "benchmarks"]
 
 [tool.black]
-target-version = ["py39", "py310"]
+target-version = ["py310", "py311", 'py312']
 skip-string-normalization = false
 line-length = 79
 ignore = ["*.yaml"]
 
-[tool.cibuildwheel]
-build = "cp39-* cp310-*"
-
-[tool.cibuildwheel.macos]
-archs = ["x86_64", "arm64"]
-
 [tool.check-manifest]
 
 [tool.pytest.ini_options]
@@ -119,13 +114,15 @@ fix = true
 [tool.tox]
 legacy_tox_ini = """
 [tox]
-envlist = py{39,310}-{coredev}
+envlist = py{310,311,312}-{coredev}
 isolated_build = True
 
 [gh-actions]
 python =
-    3.9: py39
     3.10: py310
+    3.11: py311
+    3.12: py312
+
 
 [gh-actions:env]
 # This runs the coredev environment if the "coredev" github actions input