brainglobe-atlasapi

The brainglobe atlas API (brainglobe-atlasapi) provides a common interface for programmers to download and process brain atlas data from multiple sources.

Atlases available

A number of atlases are in development, but those available currently are:

Atlas Name	Resolution	Ages	Reference Images
Allen Mouse Brain Atlas	10, 25, 50, and 100 micron	P56	STPT
Allen Human Brain Atlas	500 micron	Adult	MRI
Max Planck Zebrafish Brain Atlas	1 micron	6-dpf	FISH
Enhanced and Unified Mouse Brain Atlas	10, 25, 50, and 100 micron	P56	STPT
Smoothed version of the Kim et al. mouse reference atlas	10, 25, 50 and 100 micron	P56	STPT
Gubra's LSFM mouse brain atlas	20 micron	8 to 10 weeks post natal	LSFM
3D version of the Allen mouse spinal cord atlas	20 x 10 x 10 micron	Adult	Nissl
AZBA: A 3D Adult Zebrafish Brain Atlas	4 micron	15-16 weeks post natal	LSFM
Waxholm Space atlas of the Sprague Dawley rat brain	39 micron	P80	MRI
3D Edge-Aware Refined Atlases Derived from the Allen Developing Mouse Brain Atlases	16, 16.75, and 25 micron	E13, E15, E18, P4, P14, P28 & P56	Nissl
Princeton Mouse Brain Atlas	20 micron	>P56 (older animals included)	LSFM
Kim Lab Developmental CCF	10 micron	P56	STP, LSFM (iDISCO) and MRI (a0, adc, dwo, fa, MTR, T2)
Blind Mexican Cavefish Brain Atlas	2 micron	6 days post fertilisation	IHC
BlueBrain Barrel Cortex Atlas	10 and 25 micron	P56	STPT
UNAM Axolotl Brain Atlas	40 micron	~ 3 months post hatching	MRI

Installation

brainglobe-atlasapi works with Python >3.6, and can be installed from PyPI with:

pip install brainglobe-atlasapi

Usage

Full information can be found in the documentation

Python API

List of atlases

To see a list of atlases use brainglobe_atlasapi.show_atlases

from brainglobe_atlasapi import show_atlases
show_atlases()
#                                Brainglobe Atlases
# ╭──────────────────────────────────┬────────────┬───────────────┬──────────────╮
# │ Name                             │ Downloaded │ Local version │    Latest    │
# │                                  │            │               │   version    │
# ├──────────────────────────────────┼────────────┼───────────────┼──────────────┤
# │ allen_human_500um                │     ✔      │      0.1      │     0.1      │
# │ mpin_zfish_1um                   │     ✔      │      0.3      │     0.3      │
# │ allen_mouse_50um                 │     ✔      │      0.3      │     0.3      │
# │ kim_unified_25um                 │     ✔      │      0.1      │     0.1      │
# │ allen_mouse_25um                 │     ✔      │      0.3      │     0.3      │
# │ allen_mouse_10um                 │     ✔      │      0.3      │     0.3      │
# │ example_mouse_100um              │    ---     │      ---      │     0.3      │
# ╰──────────────────────────────────┴────────────┴───────────────┴──────────────╯

Using the atlases

All the features of each atlas can be accessed via the BrainGlobeAtlas class.

e.g. for the 25um Allen Mouse Brain Atlas:

from brainglobe_atlasapi.bg_atlas import BrainGlobeAtlas
atlas = BrainGlobeAtlas("allen_mouse_25um")

The various files associated with the atlas can then be accessed as attributes of the class:

# reference image
reference_image = atlas.reference
print(reference_image.shape)
# (528, 320, 456)

# annotation image
annotation_image = atlas.annotation
print(annotation_image.shape)
# (528, 320, 456)

# a hemispheres image (value 1 in left hemisphere, 2 in right) can be generated
hemispheres_image = atlas.hemispheres
print(hemispheres_image.shape)
# (528, 320, 456)

Brain regions

There are multiple ways to work with individual brain regions. To see a dataframe of each brain region, with it's unique ID, acronym and full name, use atlas.lookup_df:

atlas.lookup_df.head(8)
#      acronym         id                           name
# 0       root        997                           root
# 1       grey          8  Basic cell groups and regions
# 2         CH        567                       Cerebrum
# 3        CTX        688                Cerebral cortex
# 4      CTXpl        695                 Cortical plate
# 5  Isocortex        315                      Isocortex
# 6        FRP        184  Frontal pole, cerebral cortex
# 7       FRP1         68          Frontal pole, layer 1

Each brain region can also be access by the acronym, e.g. for primary visual cortex (VISp):

from pprint import pprint
VISp = atlas.structures["VISp"]
pprint(VISp)
# {'acronym': 'VISp',
#  'id': 385,
#  'mesh': None,
#  'mesh_filename': PosixPath('/home/user/.brainglobe/allen_mouse_25um_v0.3/meshes/385.obj'),
#  'name': 'Primary visual area',
#  'rgb_triplet': [8, 133, 140],
#  'structure_id_path': [997, 8, 567, 688, 695, 315, 669, 385]}

Note on coordinates in brainglobe-atlasapi

Working with both image coordinates and cartesian coordinates in the same space can be confusing! In brainglobe-atlasapi, the origin is always assumed to be in the upper left corner of the image (sectioning along the first dimension), the "ij" convention. This means that when plotting meshes and points using cartesian systems, you might encounter confusing behaviors coming from the fact that in cartesian plots one axis is inverted with respect to ij coordinates (vertical axis increases going up, image row indexes increase going down). To make things as consistent as possible, in brainglobe-atlasapi the 0 of the meshes coordinates is assumed to coincide with the 0 index of the images stack, and meshes coordinates increase following the direction stack indexes increase. To deal with transformations between your data space and brainglobe-atlasapi, you might find the brainglobe-space package helpful.

Seeking help or contributing

We are always happy to help users of our tools, and welcome any contributions. If you would like to get in contact with us for any reason, please see the contact page of our website.

Citation

If you find the BrainGlobe Atlas API useful, please cite the paper in your work:

Claudi, F., Petrucco, L., Tyson, A. L., Branco, T., Margrie, T. W. and Portugues, R. (2020). BrainGlobe Atlas API: a common interface for neuroanatomical atlases. Journal of Open Source Software, 5(54), 2668, https://doi.org/10.21105/joss.02668

Don't forget to cite the developers of the atlas that you used!

---

Atlas Generation and Adding a New Atlas

For full instructions to add a new BrainGlobe atlas, please see here.

The brainglobe_atlasapi.atlas_generation submodule contains code for the generation of cleaned-up data, for the main brainglobe_atlasapi module. This code was previously the bg-atlasgen module.

To contribute

1. Fork this repo
2. Clone your repo
3. Run git clone https://github.com/brainglobe/brainglobe-atlasapi
4. Install an editable version of the package; by running pip install -e . within the cloned directory
5. Create a script to package your atlas, and place into brainglobe_atlasapi/atlas_generation/atlas_scripts. Please see other scripts for examples.

Your script should contain everything required to run. The raw data should be hosted on a publicly accessible repository so that anyone can run the script to recreate the atlas.

If you need to add any dependencies, please add them as an extra in the pyproject.toml file, e.g.:

[project.optional-dependencies]
allenmouse = ["allensdk"]
newatlas = ["dependency_1", "dependency_2"]