diff --git a/RELEASE-NOTES.md b/RELEASE-NOTES.md
index 4009e251882a7..d4c651d7c7934 100644
--- a/RELEASE-NOTES.md
+++ b/RELEASE-NOTES.md
@@ -9,6 +9,7 @@
 1. [ISSUE #349](https://github.com/dangdangdotcom/sharding-jdbc/issues/349) ResultSet.wasNull功能不正确
 1. [ISSUE #351](https://github.com/dangdangdotcom/sharding-jdbc/issues/351) 包含在默认数据源但不在TableRule配置的表无法正确执行
 1. [ISSUE #353](https://github.com/dangdangdotcom/sharding-jdbc/issues/353) 在SQL的Where条件中兼容不是分片列的!=, !> 和 !< 操作符
+1. [ISSUE #354](https://github.com/dangdangdotcom/sharding-jdbc/issues/354) 在SQL的Where条件中兼容不是分片列的NOT操作符
 
 ## 1.5.2
 
diff --git a/sharding-jdbc-core/src/main/java/com/dangdang/ddframe/rdb/sharding/parsing/parser/clause/WhereClauseParser.java b/sharding-jdbc-core/src/main/java/com/dangdang/ddframe/rdb/sharding/parsing/parser/clause/WhereClauseParser.java
index 25de2dc796fb1..7c5050597ec7e 100644
--- a/sharding-jdbc-core/src/main/java/com/dangdang/ddframe/rdb/sharding/parsing/parser/clause/WhereClauseParser.java
+++ b/sharding-jdbc-core/src/main/java/com/dangdang/ddframe/rdb/sharding/parsing/parser/clause/WhereClauseParser.java
@@ -98,6 +98,12 @@ private void parseComparisonCondition(final ShardingRule shardingRule, final SQL
         if (lexerEngine.skipIfEqual(Symbol.LT, Symbol.LT_EQ, Symbol.GT, Symbol.GT_EQ, Symbol.LT_GT, Symbol.BANG_EQ, Symbol.BANG_GT, Symbol.BANG_LT, DefaultKeyword.LIKE)) {
             parseOtherCondition(sqlStatement);
         }
+        if (lexerEngine.skipIfEqual(DefaultKeyword.NOT)) {
+            lexerEngine.nextToken();
+            lexerEngine.skipIfEqual(Symbol.LEFT_PAREN);
+            parseOtherCondition(sqlStatement);
+            lexerEngine.skipIfEqual(Symbol.RIGHT_PAREN);
+        }
         lexerEngine.skipIfEqual(Symbol.RIGHT_PAREN);
     }
     
diff --git a/sharding-jdbc-core/src/test/resources/integrate/assert/select.xml b/sharding-jdbc-core/src/test/resources/integrate/assert/select.xml
index e526bbbe9f415..aa97a64742633 100644
--- a/sharding-jdbc-core/src/test/resources/integrate/assert/select.xml
+++ b/sharding-jdbc-core/src/test/resources/integrate/assert/select.xml
@@ -15,6 +15,11 @@
             <data parameter="100000" expected="select/SelectNotEqualsWithSingleTable.xml" />
         </sharding-rule>
     </sql>
+    <sql id="assertSelectNotEqualsWithSingleTableForNotIn">
+        <sharding-rule value="tbl">
+            <data parameter="100000" expected="select/SelectNotEqualsWithSingleTable.xml" />
+        </sharding-rule>
+    </sql>
     <sql id="assertSelectEqualsWithSameShardingColumns">
         <sharding-rule value="tbl">
             <data parameter="1000,1001" />
diff --git a/sharding-jdbc-core/src/test/resources/parser/select_expression.xml b/sharding-jdbc-core/src/test/resources/parser/select_expression.xml
index 4ae54dd14ed13..f6b21a90af13b 100644
--- a/sharding-jdbc-core/src/test/resources/parser/select_expression.xml
+++ b/sharding-jdbc-core/src/test/resources/parser/select_expression.xml
@@ -32,6 +32,14 @@
             <order-by-column name="item_id" order-by-type="ASC" />
         </order-by-columns>
     </assert>
+    <assert id="assertSelectNotEqualsWithSingleTableForNotIn" parameters="1">
+        <tables>
+            <table name="t_order_item" />
+        </tables>
+        <order-by-columns>
+            <order-by-column name="item_id" order-by-type="ASC" />
+        </order-by-columns>
+    </assert>
     <assert id="assertSelectEqualsWithSingleTable" parameters="1,1">
         <tables>
             <table name="t_order" />
diff --git a/sharding-jdbc-core/src/test/resources/sql/dql/select_expression.xml b/sharding-jdbc-core/src/test/resources/sql/dql/select_expression.xml
index cfc847dff7ccb..611632f49f17b 100644
--- a/sharding-jdbc-core/src/test/resources/sql/dql/select_expression.xml
+++ b/sharding-jdbc-core/src/test/resources/sql/dql/select_expression.xml
@@ -4,6 +4,7 @@
     <sql id="assertSelectDateFuncWithSingleTable" value="SELECT DATE(i.c_date) AS c_date FROM `t_order_item` AS i ORDER BY DATE(i.c_date) DESC" type="MySQL" />
     <sql id="assertSelectNotEqualsWithSingleTable" value="SELECT * FROM t_order_item WHERE item_id &lt;&gt; %s ORDER BY item_id" />
     <sql id="assertSelectNotEqualsWithSingleTableForExclamationEqual" value="SELECT * FROM t_order_item WHERE item_id != %s ORDER BY item_id" />
+    <sql id="assertSelectNotEqualsWithSingleTableForNotIn" value="SELECT * FROM t_order_item WHERE item_id NOT IN (%s) ORDER BY item_id" />
     <sql id="assertSelectEqualsWithSingleTable" value="SELECT * FROM t_order WHERE user_id = %s AND order_id = %s" />
     <sql id="assertSelectEqualsWithSameShardingColumns" value="SELECT * FROM t_order WHERE order_id = %s AND order_id = %s" />
     <sql id="assertSelectBetweenWithSingleTable" value="SELECT * FROM t_order WHERE user_id BETWEEN %s AND %s AND order_id BETWEEN %s AND %s ORDER BY user_id, order_id" />