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some questions of the output file #187

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ZYongQi opened this issue Jul 18, 2023 · 2 comments
Open

some questions of the output file #187

ZYongQi opened this issue Jul 18, 2023 · 2 comments

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@ZYongQi
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ZYongQi commented Jul 18, 2023

hello,this is ZYongQi !
Follow your suggestion , I have tried CNVpytor instead of CNVnator . And the output file is successfully obtained.
Part of the document is as follows:
deletion NC_048218.1:1-59000000 59000000 0.9875 0.000000e+00....
deletion NC_048218.1:26001-43000 17000 0.0403 0.000000e+00....
deletion NC_048218.1:63001-87000 24000 0.0682 0.000000e+00....
... .... .....
duplication NC_048218.1:43894001-43898000 4000 1.6724 2.559436e-04
... .... ....
deletion NC_048219.1:1-199810000 199810000 0.9863 0.000000e+00....
deletion NC_048219.1:17001-20000 3000 0.0765 5.197318e-04....
duplication NC_048219.1:347001-355000 8000 1.4984 6.103992e-10

the first question is : why it prints 'deletion' in the first line while the chromsome range prints 1-59000000 ? The fact is there is duplication in the next few line.
the second question is : after obtaining the result file and filtering it, how do you usually annotate it?
Looking forward to your reply!

@suvakov
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suvakov commented Jul 18, 2023

  1. Which caller are you using here?
  2. For annotating calls, see the following instructions.

@ZYongQi
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ZYongQi commented Aug 22, 2023

  1. Which caller are you using here?
  2. For annotating calls, see the following instructions.

This is ZYongQi!
Thank you fou your suggestion,I've sovled the problems I met before.When I got the data, I found that there were some outliers, which is noise,I mean.So I tried to filter the output calls from CNVpytor.But there is something wrong. I finally found that I have no mask file of the genome. So I wonder where did you get the mask file --mouse.strict_mask.whole_genome.fasta.gz ?

My reference is your commend on GitHub:https://github.com/abyzovlab/CNVpytor/blob/master/examples/AddReferenceGenome.md

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