Constructing 'pseudocells'

[Dillon214]

Hello DestVI devs,

I have a somewhat different question for you all. Is it possible to 'split' the transcriptome of a single spot into multiple component transcriptomes accoring to the cell types occupying it? Say, if a spot were occupied by cell type A and cell type B, could one split the transcriptome of that spot into a transcriptome A representing the contribution of cell type A, and transcriptome B representing the contribution of cell type B?

The end goal of this process, applied to all spaces, would essentially be creating a pseudo-single cell dataset. Any help regarding this would be highly appreciated. If it is possible with DestVI or other tools you all might be familiar with, I would greatly appreciate some pointers. And, if not, I would be greatly appreciative of any advice regarding the feasibility of this process, or lack thereof.

sincerely,
Dillon Brownell

[canergen]

Currently we would generate an expression per celltype. You want to split observed expression into per celltype profiles. This function exists in a branch as we will release DestVI v2 latter this year.