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 ## Input preparation
 Before testing the variability function, input data was prepared. To accomplish this, DNA sequences were download from GenBank. Then, since variability function only carry out its estimates with a single format of sequence names, names of mined sequences were restructured. Finally, only binomial system names were taken by sequence filtering. 
 
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 #### Data mining
 On this case, _Rentrez_ and _Ape_ packages were used to mine barcodes of species belonging to the family Sciaenidae from GenBank. All available ID's of COI or COX sequences, whose length is between 600-650 bp, were recruited. That interval of sequence lengths is due that barcode region have roughly 650 bp of length. Codes are shown in the following lines: 
 ```Rscript