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Add mc2 aggregation methods for biological replicates #99

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madison-feshuk opened this issue Jul 21, 2023 · 0 comments · Fixed by #108
Closed

Add mc2 aggregation methods for biological replicates #99

madison-feshuk opened this issue Jul 21, 2023 · 0 comments · Fixed by #108
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@madison-feshuk
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madison-feshuk commented Jul 21, 2023

Currently all rvals are always treated as technical replicates with no aggregation to biological replicates. Aggregation to biological replicates must occur in pre-processing steps. To address this, proposed new mc2 methods include:

  • bio.rep.mean: calculate cval as mean rval across tech replicates
  • bio.rep.median: calculate cval as median rval across tech replicates
  • bio.rep.percent: for count data, calculate cval as sum of rval / tech replicates * 100

Need to add logic for requiring minimum replicates (>1) at a concentration for zebrafish data (Could be second method)

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