GPR curation for Glycosphingolipid metabolism and Glycosylphosphatidylinositol (GPI)-anchor biosynthesis (2)

[JHL-452b]

Current behavior:

27 genes are discovered to be uncorrelated with their corresponding reactions in Glycosphingolipid metabolism and Glycosylphosphatidylinositol (GPI)-anchor biosynthesis.

Gene	UniProtID	Reaction	GPR	Correlation	Manually check
ENSG00000180549	Q11130	MAR00925: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000180549 is involved in catalyzing the transfer of L-fucose to N-acetyl glucosamine-containing structures, which is crucial for the biosynthesis of sialyl Lewis X (sLeX) and related carbohydrate structures. The catalytic activities described for this gene involve the addition of fucose to various glycosylated substrates using GDP-beta-L-fucose as a donor molecule. In contrast, the provided reaction involves the conversion of D-galactosyl-N-acylsphingosine and UDP-galactose to digalactosylceramide, UDP, and H+. This reaction involves ceramide metabolism and galactosyltransferase activity, which does not align with the fucosyltransferase functions annotated for the given gene. Hence, they are not related.	No
ENSG00000167889	Q3V5L5	MAR00925: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000167889 encodes a glycosyltransferase that acts on alpha-linked mannose of N-glycans and O-mannosyl glycans, catalyzing the transfer of N-acetylglucosamine to specific linkage structures in glycan pathways. However, the provided reaction involves the transformation of D-galactosyl-N-acylsphingosine with UDP-galactose to produce digalactosylceramide, which is not related to the glycan-modifying activities described for the gene. Therefore, there is no functional overlap between the gene's activity and the reaction provided.	No
ENSG00000102595	Q9NYU1	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000102595 encodes a protein that is involved in the quality control of protein folding in the endoplasmic reticulum by reglycosylating misfolded glycoproteins, facilitating their recognition and recycling. Its catalytic activity specifically involves reglucosylation of N-glycans. However, the reaction provided describes the conversion of glucosylceramide using UDP-galactose to produce LacCer and UDP, which is a glycosphingolipid biosynthesis pathway, not related to protein folding or reglycosylation. Thus, the gene's functional role in protein quality control does not align with the described reaction.	No
ENSG00000134910	P46977	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000134910 encodes the catalytic subunit of the oligosaccharyl transferase (OST) complex, which is involved in the initial transfer of a glycan to an asparagine residue within proteins, a process essential for protein N-glycosylation in the endoplasmic reticulum (ER). This activity involves the transfer of a glycan from dolichol-pyrophosphate to an asparagine residue. In contrast, the reaction provided involves the conversion of glucosylceramide and UDP-galactose to LacCer and UDP. This reaction is related to glycosphingolipid metabolism and does not involve N-glycosylation or the OST complex. Therefore, the gene and the reaction are not related.	No
ENSG00000163527	Q8TCJ2	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000163527 encodes a catalytic subunit of the oligosaccharyl transferase (OST) complex, which is involved in the N-glycosylation of nascent proteins within the endoplasmic reticulum. The gene's activity relates to the transfer of glycan molecules from dolichol-pyrophosphate to specific asparagine residues in proteins, a process distinct from the reaction involving glucosylceramide pool and UDP-galactose to produce LacCer pool and UDP. Consequently, the gene's function does not overlap with the specified reaction.	No
ENSG00000119227	Q86VD9	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000119227 encodes for a mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis, specifically transferring a fourth mannose to trimannosyl-GPIs. However, the reaction provided involves the conversion of glucosylceramide and UDP-galactose to H+, lactosylceramide (LacCer), and UDP, which does not involve the mannosyltransferase function because it is related to glycosylphosphatidylinositol-anchor biosynthesis, not to the metabolic pathways involving glucosylceramide and lactosylceramide.	No
ENSG00000109586	Q86SF2	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000109586 encodes a glycopeptide transferase involved specifically in O-linked oligosaccharide biosynthesis, which transfers N-acetyl-D-galactosamine to glycosylated peptides, not directly to proteins. The reactions catalyzed by this enzyme involve L-seryl-[protein] and L-threonyl-[protein] substrates with UDP-N-acetyl-alpha-D-galactosamine. The reaction provided involves glucosylceramide and UDP-galactose to produce H+, LacCer, and UDP, which does not align with the described catalytic activities of ENSG00000109586. Therefore, they are not related.	No
ENSG00000139044	Q6L9W6	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000139044 encodes a protein whose function is to transfer N-acetylgalactosamine (GalNAc) from UDP-GalNAc to N-acetylglucosamine-beta-benzyl, forming N,N'-diacetyllactosediamine structures. The catalytic activity involves converting an N-acetyl-beta-D-glucosaminyl derivative and UDP-N-acetyl-alpha-D-galactosamine to an N-acetyl-beta-D-galactosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative, H(+), and UDP. However, the reaction provided involves glucosylceramide and UDP-galactose converting to H(+), LacCer, and UDP, which is different from the reaction mediated by the gene.	No
ENSG00000156966	Q8NFL0	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000156966 encodes an N-acetyl glucosamine (GlcNAc) transferase involved in the biosynthesis and elongation of keratan sulfate proteoglycans, specifically transferring GlcNAc to galactose in N- and O-linked keratan sulfate proteoglycans. However, the reaction provided involves the conversion of glucosylceramide and UDP-galactose into LacCer pool and UDP, which does not involve the specific activity of transferring GlcNAc or the participation in keratan sulfate biosynthesis as described for the gene. Therefore, the gene and the reaction are unrelated.	No
ENSG00000060642	Q9NUD9	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000060642 is involved in glycosylphosphatidylinositol-anchor biosynthesis and functions as an alpha-1,6-mannosyltransferase which transfers the second mannose to the glycosylphosphatidylinositol during GPI precursor assembly. The reaction provided involves glucosylceramide and UDP-galactose resulting in H+, LacCer, and UDP, which is unrelated to the function of the gene. Hence, the gene and the reaction are not related.	No
ENSG00000187676	Q6Y288	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000187676 encodes for an O-glucosyltransferase that specifically transfers glucose to fucose with a beta-1,3 linkage, primarily modifying O-linked fucosylglycan on TSP type-1 domains of proteins. However, the reaction described involves the conversion of glucosylceramide and UDP-galactose to LacCer and UDP, which does not match the function of the gene described. Thus, the gene and the reaction are not related.	No
ENSG00000162885	Q8NCR0	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000162885 encodes for Beta-1,3-N-acetylgalactosaminyltransferase, which is involved in the synthesis of GalNAc-beta-1-3GlcNAc on N- and O-glycans and plays a role in alpha-dystroglycan (DAG1) glycosylation by synthesizing specific carbohydrate structures. Its catalytic activity involves the transfer of N-acetyl-alpha-D-galactosamine in specific glycosylation reactions. However, the reaction provided involves the conversion of glucosylceramide to lactosylceramide (LacCer) using UDP-galactose, which is unrelated to the gene's function of synthesizing specific glycan structures. Hence, the gene does not participate in the described reaction.	No
ENSG00000176383	Q9C0J1	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000176383 encodes for a beta-1,3-N-acetylglucosaminyltransferase, which is involved in the synthesis of poly-N-acetyllactosamine and catalyzes the reaction involving a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative and UDP-N-acetyl-alpha-D-glucosamine. However, the provided reaction involves glucosylceramide and UDP-galactose, resulting in the formation of LacCer and UDP, which is not related to the catalytic activity of the enzyme encoded by ENSG00000176383.	No
ENSG00000136731	Q9NYU2	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000136731 encodes a protein involved in the reglucosylation of glycoproteins with folding defects in the endoplasmic reticulum for quality control. Its catalytic activity is specific to the addition of glucose residues to N-glycans of misfolded proteins. The provided reaction, involving the conversion of glucosylceramide pool and UDP-galactose to LacCer pool and UDP, does not involve the specific substrates or enzymatic functions associated with ENSG00000136731, indicating that the gene and the reaction are not related.	No
ENSG00000172728	Q6P4F1	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000172728 is involved in fucosylation of N-glycan acceptors, targeting the innermost N-acetyl glucosamine (GlcNAc) residue to produce core alpha(1->3)-fucose epitopes. It specifically uses GDP-beta-L-fucose to transfer the fucosyl moiety. The reaction provided involves glucosylceramide pool and UDP-galactose to produce LacCer pool and UDP, which is unrelated to the gene's function of fucosylation.	No
ENSG00000180549	Q11130	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000180549 encodes a protein that catalyzes the transfer of L-fucose to N-acetyl glucosamine on glycoproteins or glycolipids, participating in the biosynthesis of sialyl Lewis X. The catalytic activities described involve the transfer of fucose from GDP-beta-L-fucose to various glycosylated substrates. In contrast, the reaction provided involves the transfer of galactose from UDP-galactose to glucosylceramide, forming lactosylceramide (LacCer) and UDP, and does not involve the substrates or products associated with the gene's function. Therefore, the gene and the reaction are unrelated.	No
ENSG00000085998	Q8WZA1	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000085998 is involved in O-mannosyl glycosylation and specifically catalyzes the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. It is involved in the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr structure on O-mannosylated proteins. The catalytic activity described for this gene involves the reaction of 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] with UDP-N-acetyl-alpha-D-glucosamine. However, the reaction provided involves glucosylceramide and UDP-galactose, which produces LacCer and UDP. This reaction does not match the function or catalytic activity of the gene detailed above, indicating that the gene is not relevant to the reaction provided.	No
ENSG00000198488	Q6ZMB0	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000198488 encodes for a Beta-1,3-N-acetylglucosaminyltransferase, which is involved in synthesizing the core 3 structure of O-glycans by transferring N-acetylglucosamine to proteins. The described catalytic activities involve reactions with UDP-N-acetyl-alpha-D-glucosamine to form glycoproteins. However, the provided reaction involves glucosylceramide and UDP-galactose to produce lactosylceramide (LacCer) and UDP, which is not related to the enzyme's function of synthesizing O-glycans, hence the gene is not relevant to the reaction provided.	No
ENSG00000167889	Q3V5L5	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000167889 encodes a glycosyltransferase that catalyzes the transfer of N-acetylglucosamine (GlcNAc) to mannose residues in N-glycans and O-mannosyl glycans. The reactions it catalyzes are specific to N-acetylglucosamine and involve complex branching structures in glycoproteins. In contrast, the provided reaction involves the conversion of glucosylceramide and UDP-galactose to LacCer and UDP, which is a different biochemical pathway involving different substrates and products, thus unrelated to the functions of the glycosyltransferase encoded by ENSG00000167889.	No
ENSG00000163527	Q8TCJ2	MAR00925: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000163527 encodes the catalytic subunit of the oligosaccharyl transferase (OST) complex, which is involved in the transfer of glycans to asparagine residues in nascent polypeptide chains during protein N-glycosylation. This gene's catalytic activity involves the reaction of dolichyl diphosphooligosaccharide with L-asparaginyl-[protein] to form dolichyl diphosphate and N-glycosylated protein. In contrast, the provided reaction involves D-galactosyl-N-acylsphingosine and UDP-galactose to produce digalactosylceramide, H+, and UDP, which is unrelated to protein N-glycosylation and the oligosaccharyl transferase complex functions.	Not True
ENSG00000175711	Q67FW5	MAR00925: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase that specifically catalyzes the galactosylation of cytoplasmic tRNA(Tyr) modified with queuosine at position 34, forming galactosyl-queuosine(34). The provided reaction, however, involves D-galactosyl-N-acylsphingosine and UDP-galactose to produce digalactosylceramide, H+, and UDP. These substrates and products are unrelated to the gene's specific function of galactosylating queuosine(34) in tRNA(Tyr).	No
ENSG00000172461	Q9Y231	MAR00925: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000172461 encodes for a fucosyltransferase enzyme that catalyzes the transfer of fucose from GDP-beta-L-fucose to various acceptor molecules such as N-acetyl glucosamine within type 2 lactosamine glycans. In contrast, the provided reaction involves the transfer of a galactose moiety from UDP-galactose to D-galactosyl-N-acylsphingosine, resulting in digalactosylceramide and UDP. Thus, the gene's function in fucosylation does not correspond to the reaction involving the glycosylation of ceramides by galactose transfer.	No
ENSG00000170340	Q9NY97	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000170340 encodes a beta-1,3-N-acetylglucosaminyltransferase involved in the synthesis of poly-N-acetyllactosamine, specifically catalyzing reactions involving Gal(beta1-4)Glc(NAc)-based acceptors. The described catalytic activity involves the transfer of N-acetylglucosamine to a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative, forming an N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative. However, the provided reaction describes the conversion of a glucosylceramide pool and UDP-galactose to LacCer pool, which is unrelated to the gene's function of synthesizing poly-N-acetyllactosamine. Therefore, the gene does not participate in the provided reaction.	No
ENSG00000175711	Q67FW5	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000175711 encodes a glycosyltransferase that specifically catalyzes the galactosylation of queuosine(34) in tRNA(Tyr), forming galactosyl-queuosine(34). In contrast, the provided reaction involves the conversion of glucosylceramide and UDP-galactose into LacCer and UDP, which is unrelated to the gene¡¯s function. The gene¡¯s activity is specialized for modifying tRNA, while the reaction concerns lipid metabolism.	No
ENSG00000143315	Q9H3S5	MAR00926: glucosylceramide pool + UDP-galactose --> H+ + LacCer pool + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000143315 encodes a mannosyltransferase involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis, specifically transferring the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly. The reaction provided involves the conversion of glucosylceramide and UDP-galactose into LacCer and UDP, which is unrelated to the activity and function of the mannosyltransferase encoded by the gene.	No
ENSG00000170340	Q9NY97	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000170340 encodes a Beta-1,3-N-acetylglucosaminyltransferase, which is involved in the synthesis of poly-N-acetyllactosamine and catalyzes the transfer of N-acetylglucosamine to a beta-D-galactosyl derivative. The reaction described involves D-galactosyl-N-acylsphingosine and UDP-galactose to produce digalactosylceramide, which does not match the substrate specificity or the catalytic activity annotated for the gene. Therefore, the gene and the reaction are not related.	No
ENSG00000109586	Q86SF2	MAR00924: D-galactosyl-N-acylsphingosine + UDP-galactose --> digalactosylceramide + H+ + UDP	ENSG00000016864 or ENSG00000027847 or ENSG00000060642 or ENSG00000069943 or ENSG00000085998 or ENSG00000086062 or ENSG00000102595 or ENSG00000109586 or ENSG00000117411 or ENSG00000118017 or ENSG00000118276 or ENSG00000119227 or ENSG00000120820 or ENSG00000121578 or ENSG00000124091 or ENSG00000134910 or ENSG00000136731 or ENSG00000139044 or ENSG00000143315 or ENSG00000147162 or ENSG00000148288 or ENSG00000156966 or ENSG00000158470 or ENSG00000158850 or ENSG00000162630 or ENSG00000162885 or ENSG00000163527 or ENSG00000167080 or ENSG00000167889 or ENSG00000170340 or ENSG00000172318 or ENSG00000172461 or ENSG00000172728 or ENSG00000175711 or ENSG00000176383 or ENSG00000176597 or ENSG00000177191 or ENSG00000179913 or ENSG00000180549 or ENSG00000182272 or ENSG00000183778 or ENSG00000187676 or ENSG00000196371 or ENSG00000196968 or ENSG00000198488 or ENSG00000204007 or ENSG00000237172 or ENSG00000251287	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000109586 encodes an enzyme involved in O-linked oligosaccharide biosynthesis, specifically transferring N-acetyl-D-galactosamine residues to glycosylated peptides and does not participate in the reaction transferring UDP-galactose to D-galactosyl-N-acylsphingosine to produce digalactosylceramide. The described gene activity does not correlate with the reaction provided.	No

[hhl-24]

All of these seem fine to me.

[edkerk]

All suggested changes look fine to me