GPR curation for Keratan sulfate biosynthesis

[JHL-452b]

Current behavior:

31 genes are discovered to be uncorrelated with their corresponding reactions in Keratan sulfate biosynthesis.

Gene	UniProtID	Reaction	GPR	Correlation	Manually check
ENSG00000175711	Q67FW5	MAR07336: keratan sulfate I biosynthesis, precursor 1 + UDP-N-acetylglucosamine --> H+ + keratan sulfate I biosynthesis, precursor 2 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase that specifically catalyzes the galactosylation of queuosine(34) in tRNA(Tyr) using UDP-alpha-D-galactose, as outlined in its annotation. This reaction results in the formation of O-5''-beta-D-galactosylqueuosine(34) in tRNA(Tyr). However, the provided reaction involves the synthesis of keratan sulfate I, where a precursor reacts with UDP-N-acetylglucosamine. These two reactions involve different substrates and products, indicating that the gene's function and catalytic activity are not related to the provided keratan sulfate I biosynthesis reaction.	No
ENSG00000175711	Q67FW5	MAR07338: keratan sulfate I biosynthesis, precursor 3 + UDP-N-acetylglucosamine --> H+ + keratan sulfate I biosynthesis, precursor 4 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes for a glycosyltransferase that specifically catalyzes the galactosylation of tRNA(Tyr) modified with queuosine at position 34, forming galactosyl-queuosine(34). However, the provided reaction involves the biosynthesis of keratan sulfate I and the conversion of its precursor 3 to precursor 4, which involves UDP-N-acetylglucosamine, and does not relate to the function of the gene ENSG00000175711 in galactosylating tRNA(Tyr).	No
ENSG00000135702	Q9GZS9	MAR07339: keratan sulfate I biosynthesis, precursor 4 + PAPS --> H+ + keratan sulfate I biosynthesis, precursor 5 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000135702 encodes a sulfotransferase that specifically transfers sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. It does not act on longer carbohydrate substrates with poly-N-acetyllactosamine structures and has no activity toward keratan. The reaction provided involves the biosynthesis of keratan sulfate I, which is not a substrate acted upon by the sulfotransferase encoded by this gene. Therefore, there is no relevance between the specified gene and the given reaction.	No
ENSG00000175711	Q67FW5	MAR07341: keratan sulfate I biosynthesis, precursor 6 + UDP-N-acetylglucosamine --> H+ + keratan sulfate I biosynthesis, precursor 7 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase that specifically catalyzes the galactosylation of cytoplasmic tRNA(Tyr) modified with queuosine at position 34. Its catalytic activity involves converting queuosine(34) in tRNA(Tyr) and UDP-alpha-D-galactose to O-5''-beta-D-galactosyl queuosine(34) in tRNA(Tyr) and UDP. In contrast, the provided reaction involves a different biochemical pathway, specifically the biosynthesis of keratan sulfate I, which converts precursor 6 and UDP-N-acetylglucosamine to precursor 7 and UDP. Since the gene's function does not overlap with the pathway described in the provided reaction, they are not related.	No
ENSG00000135702	Q9GZS9	MAR07345: keratan sulfate I biosynthesis, precursor 10 + PAPS --> H+ + keratan sulfate I biosynthesis, precursor 11 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000135702 codes for a sulfotransferase that specifically catalyzes the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. However, it does not transfer sulfate to longer carbohydrate substrates with poly-N-acetyllactosamine structures and has no activity toward keratan. The reaction in question involves keratan sulfate I biosynthesis, which does not align with the specific activities and substrate specificities of the sulfotransferase encoded by ENSG00000135702. Hence, the gene and the reaction are not related.	No
ENSG00000175711	Q67FW5	MAR07347: keratan sulfate I biosynthesis, precursor 12 + UDP-N-acetylglucosamine --> H+ + keratan sulfate I biosynthesis, precursor 13 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000175711 encodes a glycosyltransferase that specifically catalyzes the galactosylation of cytoplasmic tRNA(Tyr) modified with queuosine at position 34, forming galactosyl-queuosine(34). Meanwhile, the reaction provided involves the biosynthesis of keratan sulfate I, where UDP-N-acetylglucosamine reacts to produce keratan sulfate I biosynthesis precursor and H+. Therefore, the gene is involved in a different glycosylation process (tRNA modification) and not in the biosynthesis of keratan sulfate I.	No
ENSG00000135702	Q9GZS9	MAR07351: keratan sulfate I biosynthesis, precursor 16 + PAPS --> H+ + keratan sulfate I biosynthesis, precursor 17 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000135702 codes for a sulfotransferase that catalyzes the transfer of sulfate to the 6-position of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. It specifically acts on short carbohydrate substrates and has no activity toward keratan. The reaction provided involves keratan sulfate I biosynthesis, which requires the transfer of sulfate to these structures. Since the gene product does not act on keratan or longer carbohydrate substrates and is not involved in the relevant biosynthesis pathway, it is not related to the provided reaction.	No
ENSG00000135702	Q9GZS9	MAR07354: keratan sulfate I biosynthesis, precursor 19 + PAPS --> H+ + keratan sulfate I biosynthesis, precursor 20 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000135702 encodes a sulfotransferase enzyme that specifically catalyzes the sulfate transfer to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors, but it has no activity towards longer carbohydrate substrates with poly-N-acetyllactosamine structures and keratan. The reaction provided involves keratan sulfate I biosynthesis, which the gene's enzyme cannot process, highlighting the irrelevance.	No
ENSG00000135702	Q9GZS9	MAR07357: keratan sulfate I biosynthesis, precursor 22 + PAPS --> H+ + keratan sulfate I biosynthesis, precursor 23 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000135702 codes for a sulfotransferase that uses 3'-phospho-5'-adenylyl sulfate (PAPS) to transfer sulfate to GlcNAc residues and O-linked sugars of mucin-type acceptors, specifically targeting short carbohydrate substrates. It has no activity toward keratan, the substrate involved in the provided reaction for keratan sulfate I biosynthesis. Therefore, the given gene does not catalyze the reaction related to keratan sulfate I biosynthesis.	No
ENSG00000175711	Q67FW5	MAR07359: keratan sulfate I biosynthesis, precursor 24 + UDP-N-acetylglucosamine --> H+ + keratan sulfate I biosynthesis, precursor 25 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 codes for a glycosyltransferase that specifically catalyzes the galactosylation of cytoplasmic tRNA(Tyr) modified with queuosine at position 34, forming galactosyl-queuosine(34) in tRNA(Tyr). The catalytic activity described involves the transfer of a galactose from UDP-alpha-D-galactose to queuosine(34). In contrast, the provided reaction involves the biosynthesis of keratan sulfate I, which includes transferring an N-acetylglucosamine from UDP-N-acetylglucosamine to the keratan sulfate precursor. These processes are distinct in terms of substrate specificity and biological functions, indicating that the gene ENSG00000175711 is unrelated to the provided reaction.	No
ENSG00000135702	Q9GZS9	MAR07360: keratan sulfate I biosynthesis, precursor 25 + PAPS --> H+ + keratan sulfate I biosynthesis, precursor 26 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000135702 encodes a sulfotransferase enzyme that does not have activity toward keratan and does not transfer sulfate to longer carbohydrate substrates with poly-N-acetyllactosamine structures. In contrast, the reaction mentioned involves keratan sulfate I biosynthesis. Therefore, due to the gene's specificity and lack of activity towards keratan, it is not related to the provided reaction.	No
ENSG00000175711	Q67FW5	MAR07362: keratan sulfate I biosynthesis, precursor 27 + UDP-N-acetylglucosamine --> H+ + keratan sulfate I biosynthesis, precursor 28 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000175711 encodes a glycosyltransferase that catalyzes the galactosylation of cytoplasmic tRNA(Tyr), specifically modifying queuosine at position 34, forming galactosyl-queuosine(34). This reaction involves UDP-alpha-D-galactose and is associated with protein translation regulation. However, the reaction mentioned in the provided text involves the biosynthesis of keratan sulfate I, using UDP-N-acetylglucosamine, which is unrelated to the function of the gene ENSG00000175711.	No
ENSG00000135702	Q9GZS9	MAR07363: keratan sulfate I biosynthesis, precursor 28 + PAPS --> H+ + keratan sulfate I biosynthesis, precursor 29 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000135702 encodes a sulfotransferase that transfers sulfate to the position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors, and it specifically does not have activity towards keratan. The described reaction involves the biosynthesis of keratan sulfate, which this gene's product does not influence, making it irrelevant to the reaction specified.	No
ENSG00000175711	Q67FW5	MAR07365: keratan sulfate I biosynthesis, precursor 30 + UDP-N-acetylglucosamine --> H+ + keratan sulfate I biosynthesis, precursor 31 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 has a function related to glycosyltransferase activity which catalyzes the galactosylation of queuosine(34) in tRNA(Tyr), as specified. This gene's activity involves the reaction of queuosine(34) in tRNA(Tyr) with UDP-alpha-D-galactose, resulting in O-5''-beta-D-galactosylqueuosine(34) in tRNA(Tyr) and UDP. In contrast, the provided reaction involves keratan sulfate I biosynthesis and UDP-N-acetylglucosamine, which is unrelated to the specific function and catalytic activity of the gene described. Therefore, the gene ENSG00000175711 does not participate in the keratan sulfate I biosynthesis reaction.	No
ENSG00000135702	Q9GZS9	MAR07366: keratan sulfate I biosynthesis, precursor 31 + PAPS --> H+ + keratan sulfate I biosynthesis, precursor 32 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000135702 encodes a sulfotransferase enzyme that utilizes PAPS to transfer sulfate to non-reducing N-acetylglucosamine residues and O-linked sugars of mucin-type acceptors, with no activity toward keratan or longer carbohydrate substrates with poly-N-acetyllactosamine structures. The reaction provided involves keratan sulfate I biosynthesis, which is outside the substrate specificity of this gene, indicating no relevance to the reaction provided.	No
ENSG00000175711	Q67FW5	MAR07368: keratan sulfate I biosynthesis, precursor 33 + UDP-N-acetylglucosamine --> H+ + keratan sulfate I biosynthesis, precursor 34 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase specifically involved in the galactosylation of tRNA(Tyr) queuosine(34), forming galactosyl-queuosine(34) using UDP-alpha-D-galactose. In contrast, the provided reaction is involved in keratan sulfate I biosynthesis, which utilizes UDP-N-acetylglucosamine. These processes involve different biochemical functions and substrates, indicating that the gene does not participate in the keratan sulfate I biosynthesis reaction.	No
ENSG00000175711	Q67FW5	MAR07444: keratan sulfate II biosynthesis, precursor 2 + UDP-N-acetylglucosamine --> H+ + keratan sulfate II biosynthesis, precursor 3 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase involved in the galactosylation of cytoplasmic tRNA(Tyr) at position 34 with queuosine. Its specific catalytic activity involves galactosylation using UDP-alpha-D-galactose. However, the reaction in question involves the synthesis of keratan sulfate II using UDP-N-acetylglucosamine, which is a different substrate and biochemical process. Thus, the gene and the reaction are not related.	No
ENSG00000175711	Q67FW5	MAR07446: keratan sulfate II biosynthesis, precursor 4 + UDP-N-acetylglucosamine --> H+ + keratan sulfate II biosynthesis, precursor 5 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase that specifically catalyzes the galactosylation of queuosine(34) in tRNA(Tyr). The reaction detailed in the gene's annotation involves UDP-alpha-D-galactose and queuosine(34) in tRNA(Tyr), resulting in the formation of galactosyl-queuosine(34) in tRNA(Tyr) and UDP. The provided reaction, however, involves the biosynthesis of keratan sulfate II, which includes precursor 4 and UDP-N-acetylglucosamine as substrates and results in precursor 5 and UDP. Since the substrates and products of the gene's annotated catalytic activity do not match those of the provided reaction, the gene is not relevant to this reaction.	No
ENSG00000175711	Q67FW5	MAR07449: keratan sulfate II biosynthesis, precursor 7 + UDP-N-acetylglucosamine --> H+ + keratan sulfate II biosynthesis, precursor 8 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase enzyme involved specifically in the galactosylation of queuosine-modified tRNA(Tyr). It catalyzes the reaction where queuosine(34) in tRNA(Tyr) and UDP-alpha-D-galactose are converted to galactosyl-queuosine(34) in tRNA(Tyr) and UDP. However, the reaction provided involves the biosynthesis of keratan sulfate II, where a precursor reacts with UDP-N-acetylglucosamine to form the next precursor in the pathway, indicating distinct substrates and products from the gene's known function. Therefore, the gene's annotated function does not relate to the described reaction.	No
ENSG00000175711	Q67FW5	MAR07473: keratan sulfate II (core 4-linked), biosynthesis, precursor 2 + UDP-N-acetylglucosamine --> H+ + keratan sulfate II (core 4-linked), biosynthesis, precursor 3 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase enzyme that specifically catalyzes the galactosylation of queuosine(34) in tRNA(Tyr). It converts queuosine(34) in tRNA(Tyr) and UDP-alpha-D-galactose into O-5''-beta-D-galactosylqueuosine(34) in tRNA(Tyr) and UDP. The provided reaction involves the biosynthesis of keratan sulfate II, where UDP-N-acetylglucosamine is used to elongate the keratan sulfate chain. Since the gene's function and catalytic activity are specific to tRNA modification and not related to keratan sulfate biosynthesis, the gene is not relevant to the reaction provided.	No
ENSG00000175711	Q67FW5	MAR07475: keratan sulfate II (core 4-linked), biosynthesis, precursor 4 + UDP-N-acetylglucosamine --> H+ + keratan sulfate II (core 4-linked), biosynthesis, precursor 5 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000175711 encodes a glycosyltransferase that specifically catalyzes the galactosylation of cytoplasmic tRNA(Tyr) modified with queuosine at position 34, forming galactosyl-queuosine(34) in tRNA(Tyr). The annotated reaction involves queuosine(34) in tRNA(Tyr) and UDP-alpha-D-galactose. Contrarily, the provided reaction involves keratan sulfate II (core 4-linked) and UDP-N-acetylglucosamine, which is unrelated to the specific function of the gene product described.	No
ENSG00000135702	Q9GZS9	MAR07479: keratan sulfate II (core 4-linked), biosynthesis, precursor 8 + PAPS --> H+ + keratan sulfate II (core 4-linked), biosynthesis, precursor 9 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000135702 encodes for a sulfotransferase that specifically transfers sulfate to the position 6 of non-reducing N-acetylglucosamine residues and O-linked sugars of mucin-type acceptors, but does not act on keratan or longer carbohydrate substrates with poly-N-acetyllactosamine structures. The reaction described involves the sulfation of keratan sulfate II, which this sulfotransferase does not have activity toward.	No
ENSG00000175711	Q67FW5	MAR07353: keratan sulfate I biosynthesis, precursor 18 + UDP-N-acetylglucosamine --> H+ + keratan sulfate I biosynthesis, precursor 19 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase that catalyzes the galactosylation of cytoplasmic tRNA(Tyr) at the queuosine(34) position with UDP-alpha-D-galactose. The provided reaction involves the biosynthesis of keratan sulfate I, which requires UDP-N-acetylglucosamine. These are two different biochemical processes, involving different substrates and products, which indicates that the gene's function does not relate to the provided reaction.	No
ENSG00000135702	Q9GZS9	MAR07342: keratan sulfate I biosynthesis, precursor 7 + PAPS --> H+ + keratan sulfate I biosynthesis, precursor 8 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000135702 encodes a sulfotransferase that uses PAPS to transfer sulfate to GlcNAc residues and O-linked sugars of mucin-type acceptors. However, it does not act on longer carbohydrate substrates with poly-N-acetyllactosamine structures nor does it have activity towards keratan. The reaction described involves the conversion of keratan sulfate I biosynthesis precursor, which is not a recognized substrate for the encoded enzyme. Therefore, the gene and the reaction are not related.	No
ENSG00000175711	Q67FW5	MAR07344: keratan sulfate I biosynthesis, precursor 9 + UDP-N-acetylglucosamine --> H+ + keratan sulfate I biosynthesis, precursor 10 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase that specifically catalyzes the galactosylation of cytoplasmic tRNA(Tyr) modified with queuosine at position 34, forming galactosyl-queuosine(34). Its catalytic activity involves the reaction of queuosine(34) in tRNA(Tyr) with UDP-alpha-D-galactose to produce O-5''-beta-D-galactosylqueuosine(34) in tRNA(Tyr) and UDP. However, the provided reaction involves the biosynthesis of keratan sulfate I, requiring UDP-N-acetylglucosamine to produce keratan sulfate I biosynthesis precursor 10 and UDP, which is unrelated to the gene¡¯s specific function in galactosylation of tRNA(Tyr).	No
ENSG00000135702	Q9GZS9	MAR07348: keratan sulfate I biosynthesis, precursor 13 + PAPS --> H+ + keratan sulfate I biosynthesis, precursor 14 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. Based on the annotation, the gene ENSG00000135702 encodes a sulfotransferase enzyme that specifically catalyzes the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors but not to longer carbohydrate substrates that have poly-N-acetyllactosamine structures or keratan. The given reaction involves keratan sulfate I biosynthesis, for which the gene-encoded enzyme has no activity. Therefore, the gene is not relevant to this specific reaction.	No
ENSG00000175711	Q67FW5	MAR07350: keratan sulfate I biosynthesis, precursor 15 + UDP-N-acetylglucosamine --> H+ + keratan sulfate I biosynthesis, precursor 16 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase that specifically catalyzes the galactosylation of cytoplasmic tRNA(Tyr) modified with queuosine at position 34, forming galactosyl-queuosine(34). The annotated gene function and catalytic activity do not involve UDP-N-acetylglucosamine or keratan sulfate I biosynthesis as mentioned in the provided reaction, indicating that the gene and the reaction are not related.	No
ENSG00000175711	Q67FW5	MAR07356: keratan sulfate I biosynthesis, precursor 21 + UDP-N-acetylglucosamine --> H+ + keratan sulfate I biosynthesis, precursor 22 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase that specifically catalyzes the galactosylation of queuosine(34) in tRNA(Tyr). The provided reaction pertains to keratan sulfate I biosynthesis involving UDP-N-acetylglucosamine. Therefore, the gene ENSG00000175711 and the reaction provided are not related, as they involve different substrates and biological processes.	No
ENSG00000135702	Q9GZS9	MAR07447: keratan sulfate II biosynthesis, precursor 5 + PAPS --> H+ + keratan sulfate II biosynthesis, precursor 6 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000135702 encodes a sulfotransferase that catalyzes the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors, specifically targeting short carbohydrate substrates and showing no activity toward keratan. The provided reaction involves the biosynthesis of keratan sulfate II, which is not a substrate for the enzyme encoded by this gene, explaining the lack of relevance.	No
ENSG00000135702	Q9GZS9	MAR07476: keratan sulfate II (core 4-linked), biosynthesis, precursor 5 + PAPS --> H+ + keratan sulfate II (core 4-linked), biosynthesis, precursor 6 + PAP	ENSG00000135702 or ENSG00000140835 or ENSG00000175040 or ENSG00000183196	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000135702 encodes for a sulfotransferase that catalyzes the transfer of sulfate to the terminal GlcNAc residues and O-linked sugars of mucin-type acceptors, but specifically does not have activity toward keratan or longer carbohydrate substrates with poly-N-acetyllactosamine structures. The reaction described involves the biosynthesis of keratan sulfate II, which the gene's product does not act upon, thus making the gene unrelated to the reaction.	No
ENSG00000175711	Q67FW5	MAR07478: keratan sulfate II (core 4-linked), biosynthesis, precursor 7 + UDP-N-acetylglucosamine --> H+ + keratan sulfate II (core 4-linked), biosynthesis, precursor 8 + UDP	ENSG00000156966 or ENSG00000170340 or ENSG00000174684 or ENSG00000175711 or ENSG00000176383 or ENSG00000177191 or ENSG00000179913	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175711 encodes a glycosyltransferase that specifically catalyzes the galactosylation of queuosine(34) in tRNA(Tyr) using UDP-alpha-D-galactose. In contrast, the provided reaction involves the biosynthesis of keratan sulfate II using UDP-N-acetylglucosamine. The different substrates (UDP-alpha-D-galactose vs. UDP-N-acetylglucosamine) and target molecules (tRNA(Tyr) vs. keratan sulfate II) indicate that the gene's function is not related to the reaction described.	No

[feiranl]

LGTM! This issue mainly fixes the wrong substrates for two genes: ENSG00000175711 and ENSG00000135702.

[JHL-452b]

Fixed by #907