GPR curation for Phenylalanine, tyrosine and tryptophan biosynthesis/Porphyrin metabolism

[JHL-452b]

Current behavior:

28 genes are discovered to be uncorrelated with their corresponding reactions in Phenylalanine, tyrosine and tryptophan biosynthesis/Porphyrin metabolism

Gene	UniProtID	Reaction	GPR	Correlation	Manually check
ENSG00000184254	P47895	MAR06749: 3-methoxy-4-hydroxyphenylglycolaldehyde + H2O + NADP+ --> 2.0 H+ + NADPH + vanillylmandelate	ENSG00000006534 or ENSG00000108602 or ENSG00000132746 or ENSG00000184254	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000184254 encodes an enzyme that catalyzes the NAD-dependent oxidation of aldehyde substrates such as all-trans-retinal and all-trans-13,14-dihydroretinal to their corresponding carboxylic acids. It has high specificity for all-trans-retinal as a substrate and is involved in the biosynthesis of retinoate, a critical lipid in embryonic development. In contrast, the provided reaction involves the conversion of 3-methoxy-4-hydroxyphenylglycolaldehyde to vanillylmandelate in the presence of NADP+, which is not one of the substrates or products associated with the enzyme encoded by this gene. Therefore, the gene and the reaction are not related.	No
ENSG00000184254	P47895	MAR06754: 3,4-dihydroxymandelaldehyde + H2O + NADP+ --> 3,4-dihydroxymandelate + 2.0 H+ + NADPH	ENSG00000006534 or ENSG00000108602 or ENSG00000132746 or ENSG00000184254	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000184254 catalyzes the NAD-dependent oxidation of aldehyde substrates like all-trans-retinal and all-trans-13,14-dihydroretinal to their corresponding carboxylic acids. It shows high specificity for all-trans-retinal and acetaldehyde. The provided reaction involves 3,4-dihydroxymandelaldehyde, NADP+, and produces 3,4-dihydroxymandelate, which is not among the substrates or products listed for the gene ENSG00000184254. Hence, the gene and the reaction are not related.	No
ENSG00000184254	P47895	MAR06761: 3,4-dihydroxyphenylacetaldehyde + H2O + NADP+ --> 3,4-dihydroxyphenylacetate + 2.0 H+ + NADPH	ENSG00000006534 or ENSG00000108602 or ENSG00000132746 or ENSG00000184254	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000184254 catalyzes the NAD-dependent oxidation of aldehyde substrates like all-trans-retinal and all-trans-13,14-dihydroretinal to their corresponding carboxylic acids, all-trans-retinoate and all-trans-13,14-dihydroretinoate, respectively. However, the reaction provided involves the conversion of 3,4-dihydroxyphenylacetaldehyde to 3,4-dihydroxyphenylacetate using NADP+ as a cofactor, which falls outside the substrate specificity and cofactor utilization (NAD+ vs. NADP+) of the gene ENSG00000184254. Therefore, the gene is not relevant to the reaction provided.	No
ENSG00000184254	P47895	MAR06767: 3-methoxy-4-hydroxyphenylacetaldehyde + H2O + NADP+ --> 2.0 H+ + homovanillate + NADPH	ENSG00000006534 or ENSG00000108602 or ENSG00000132746 or ENSG00000184254	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000184254 encodes for an enzyme that catalyzes the NAD-dependent oxidation of aldehyde substrates such as all-trans-retinal and all-trans-13,14-dihydroretinal to their corresponding carboxylic acids. The reaction given involves 3-methoxy-4-hydroxyphenylacetaldehyde, H2O, and NADP+, producing homovanillate and NADPH, which does not align with the enzyme's known substrates and reaction mechanisms. Therefore, the gene and the reaction are not related.	No
ENSG00000184254	P47895	MAR06791: 4-hydroxyphenylacetaldehyde + H2O + NADP+ --> 4-hydroxyphenylacetate + 2.0 H+ + NADPH	ENSG00000006534 or ENSG00000108602 or ENSG00000132746 or ENSG00000184254	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000184254 encodes an enzyme that catalyzes the NAD-dependent oxidation of aldehyde substrates like all-trans-retinal and all-trans-13,14-dihydroretinal to their corresponding carboxylic acids, and has high specificity for all-trans-retinal. However, the provided reaction involves the conversion of 4-hydroxyphenylacetaldehyde to 4-hydroxyphenylacetate with NADP+ as a cofactor, not NAD+. Thus, the enzymatic function of the gene does not align with the reaction described.	No
ENSG00000130508	Q92626	MAR06808: dopamine + H2O2 --> dopamine-O-quinone + 2.0 H2O	ENSG00000117592 or ENSG00000121053 or ENSG00000130508 or ENSG00000167419	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000130508 encodes for an enzyme that primarily catalyzes the oxidation of bromide, thiocyanate, and iodide, and is involved in the formation of sulfilimine cross-links within collagen and other related reactions. These activities are crucial for extracellular matrix assembly and signaling. However, the provided reaction involves the oxidation of dopamine by hydrogen peroxide to form dopamine-O-quinone, a reaction not mentioned in the gene's function or catalytic activities. Therefore, the gene's annotated functions and activities do not align with the provided reaction.	No
ENSG00000117592	P30041	MAR06813: dopamine-O-quinone + H2O2 --> dopaminochrome + H+ + 2.0 H2O	ENSG00000005381 or ENSG00000115705 or ENSG00000117592 or ENSG00000121053 or ENSG00000167419	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000117592 encodes a thiol-specific peroxidase that primarily catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols. Additionally, it exhibits phospholipase and acyltransferase activities related to phospholipid metabolism and protection against oxidative stress. However, the provided reaction involves the conversion of dopamine-O-quinone to dopaminochrome in the presence of hydrogen peroxide, which does not align with any of the described catalytic activities of the gene's encoded enzyme. Therefore, based on the annotated functions and catalytic activities, the gene is not involved in the reaction provided.	No
ENSG00000241119	O60656	MAR06828: 3,3-diiodo-L-thyronine + UDP-glucuronate --> 3,3-diiodo-L-thyronine-beta-D-glucuronoside + H+ + UDP	ENSG00000109181 or ENSG00000135220 or ENSG00000135226 or ENSG00000145626 or ENSG00000156096 or ENSG00000167165 or ENSG00000168671 or ENSG00000171234 or ENSG00000173610 or ENSG00000196620 or ENSG00000197888 or ENSG00000213759 or ENSG00000288705 or ENSG00000241119 or ENSG00000241635 or ENSG00000242366 or ENSG00000242515 or ENSG00000288702 or ENSG00000244122 or ENSG00000244474	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000241119, which encodes for UDP-glucuronosyltransferase (UGT), catalyzes the glucuronidation of a variety of substrates including endogenous estrogens, phytoestrogens, drugs, and xenobiotics to facilitate their excretion. The enzyme¡¯s known reactions include glucuronidation of hormones like estradiol, substrates like prunetin, candesartan, SN-38, and mycophenolate. However, the provided reaction involves the glucuronidation of 3,3-diiodo-L-thyronine, a substrate not mentioned in the gene's annotation. Therefore, this specific gene does not appear to be involved in the reaction of converting 3,3-diiodo-L-thyronine to its glucuronoside form.	No Yes
ENSG00000166507	P52849	MAR06829: 3,3-diiodo-L-thyronine + PAPS --> 3,3-diiodo-L-thyronine-4-O-sulfate + H+ + PAP	ENSG00000070614 or ENSG00000111962 or ENSG00000115526 or ENSG00000124302 or ENSG00000130540 or ENSG00000135702 or ENSG00000136720 or ENSG00000138653 or ENSG00000140835 or ENSG00000147119 or ENSG00000153936 or ENSG00000154080 or ENSG00000154252 or ENSG00000164100 or ENSG00000166507 or ENSG00000171004 or ENSG00000173597 or ENSG00000175040 or ENSG00000175229 or ENSG00000183196 or ENSG00000185352 or ENSG00000197093 or ENSG00000198075 or ENSG00000198203	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000166507 encodes an enzyme that catalyzes the N-deacetylation and N-sulfation of glucosamine in heparan sulfate, which is distinct from the reaction provided (3,3-diiodo-L-thyronine + PAPS --> 3,3-diiodo-L-thyronine-4-O-sulfate + H+ + PAP) involving the sulfation of 3,3-diiodo-L-thyronine. Therefore, the gene's function does not align with the reaction specified.	No
ENSG00000140835	Q8NCG5	MAR06829: 3,3-diiodo-L-thyronine + PAPS --> 3,3-diiodo-L-thyronine-4-O-sulfate + H+ + PAP	ENSG00000070614 or ENSG00000111962 or ENSG00000115526 or ENSG00000124302 or ENSG00000130540 or ENSG00000135702 or ENSG00000136720 or ENSG00000138653 or ENSG00000140835 or ENSG00000147119 or ENSG00000153936 or ENSG00000154080 or ENSG00000154252 or ENSG00000164100 or ENSG00000166507 or ENSG00000171004 or ENSG00000173597 or ENSG00000175040 or ENSG00000175229 or ENSG00000183196 or ENSG00000185352 or ENSG00000197093 or ENSG00000198075 or ENSG00000198203	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000140835 encodes a sulfotransferase enzyme involved in the biosynthesis of SELL/L-selectin ligands. Its primary function is to catalyze the transfer of sulfate groups to specific GlcNAc residues within O-linked mucin-type glycans. This activity is essential for the formation of sialyl 6-sulfo Lewis X determinants used in lymphocyte homing. The provided reaction, on the other hand, involves the sulfation of 3,3-diiodo-L-thyronine (an iodothyronine), which is different from the O-linked glycan substrates that the gene's encoded enzyme acts upon. Therefore, the gene's function is not relevant to the reaction involving 3,3-diiodo-L-thyronine and PAPS.	No
ENSG00000138653	Q9H3R1	MAR06829: 3,3-diiodo-L-thyronine + PAPS --> 3,3-diiodo-L-thyronine-4-O-sulfate + H+ + PAP	ENSG00000070614 or ENSG00000111962 or ENSG00000115526 or ENSG00000124302 or ENSG00000130540 or ENSG00000135702 or ENSG00000136720 or ENSG00000138653 or ENSG00000140835 or ENSG00000147119 or ENSG00000153936 or ENSG00000154080 or ENSG00000154252 or ENSG00000164100 or ENSG00000166507 or ENSG00000171004 or ENSG00000173597 or ENSG00000175040 or ENSG00000175229 or ENSG00000183196 or ENSG00000185352 or ENSG00000197093 or ENSG00000198075 or ENSG00000198203	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000138653 encodes an enzyme responsible for the N-deacetylation and N-sulfation of glucosamine in heparan sulfate, with a catalytic activity involving 3'-phosphoadenylyl sulfate and alpha-D-glucosaminyl-heparan sulfate(n). In contrast, the reaction provided involves the substrate 3,3-diiodo-L-thyronine and PAPS, producing 3,3-diiodo-L-thyronine-4-O-sulfate. These processes involve different substrates and products, indicating that the gene's function does not align with the reaction provided.	No
ENSG00000136720	O60243	MAR06829: 3,3-diiodo-L-thyronine + PAPS --> 3,3-diiodo-L-thyronine-4-O-sulfate + H+ + PAP	ENSG00000070614 or ENSG00000111962 or ENSG00000115526 or ENSG00000124302 or ENSG00000130540 or ENSG00000135702 or ENSG00000136720 or ENSG00000138653 or ENSG00000140835 or ENSG00000147119 or ENSG00000153936 or ENSG00000154080 or ENSG00000154252 or ENSG00000164100 or ENSG00000166507 or ENSG00000171004 or ENSG00000173597 or ENSG00000175040 or ENSG00000175229 or ENSG00000183196 or ENSG00000185352 or ENSG00000197093 or ENSG00000198075 or ENSG00000198203	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000136720 encodes a 6-O-sulfation enzyme involved in the transfer of sulfate to the N-sulfoglucosamine residue of heparan sulfate, whereas the provided reaction involves the sulfation of 3,3-diiodo-L-thyronine at the 4-O position. The substrates and products in the gene's function and the given reaction do not match, indicating that the gene and the reaction are not related.	No
ENSG00000124302	Q9H2A9	MAR06829: 3,3-diiodo-L-thyronine + PAPS --> 3,3-diiodo-L-thyronine-4-O-sulfate + H+ + PAP	ENSG00000070614 or ENSG00000111962 or ENSG00000115526 or ENSG00000124302 or ENSG00000130540 or ENSG00000135702 or ENSG00000136720 or ENSG00000138653 or ENSG00000140835 or ENSG00000147119 or ENSG00000153936 or ENSG00000154080 or ENSG00000154252 or ENSG00000164100 or ENSG00000166507 or ENSG00000171004 or ENSG00000173597 or ENSG00000175040 or ENSG00000175229 or ENSG00000183196 or ENSG00000185352 or ENSG00000197093 or ENSG00000198075 or ENSG00000198203	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000124302 encodes an enzyme that catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans, and it is required for the biosynthesis of glycoprotein hormones such as lutropin and thyrotropin. The reaction provided involves the sulfation of 3,3-diiodo-L-thyronine using PAPS, which is a different substrate and product than those associated with the enzyme described. Therefore, the gene's annotated function does not match the reaction in question.	No
ENSG00000183196	Q9GZX3	MAR06829: 3,3-diiodo-L-thyronine + PAPS --> 3,3-diiodo-L-thyronine-4-O-sulfate + H+ + PAP	ENSG00000070614 or ENSG00000111962 or ENSG00000115526 or ENSG00000124302 or ENSG00000130540 or ENSG00000135702 or ENSG00000136720 or ENSG00000138653 or ENSG00000140835 or ENSG00000147119 or ENSG00000153936 or ENSG00000154080 or ENSG00000154252 or ENSG00000164100 or ENSG00000166507 or ENSG00000171004 or ENSG00000173597 or ENSG00000175040 or ENSG00000175229 or ENSG00000183196 or ENSG00000185352 or ENSG00000197093 or ENSG00000198075 or ENSG00000198203	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000183196 encodes a sulfotransferase that catalyzes the transfer of sulfate to N-acetylglucosamine residues in keratan sulfate using PAPS as a donor. This is involved in the biosynthesis and elongation of keratan sulfate. However, the provided reaction describes the sulfation of 3,3-diiodo-L-thyronine to form 3,3-diiodo-L-thyronine-4-O-sulfate, which is unrelated to the function of the sulfotransferase encoded by the gene. Therefore, the gene and the reaction are not related.	No
ENSG00000167419	P22079	MAR07628: O2 + phenylalanine --> 2-phenylacetamide + CO2 + H2O	ENSG00000117592 or ENSG00000121053 or ENSG00000130508 or ENSG00000167419	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000167419 codes for a heme-containing oxidoreductase involved in the conversion of thiocyanate and iodide in the presence of hydrogen peroxide, contributing to antimicrobial defense and the regulation of hydrogen peroxide levels. The catalytic activities described do not include or relate to the conversion of phenylalanine to 2-phenylacetamide, CO2, and H2O. Thus, the gene and the reaction are not related.	No
ENSG00000117592	P30041	MAR07628: O2 + phenylalanine --> 2-phenylacetamide + CO2 + H2O	ENSG00000117592 or ENSG00000121053 or ENSG00000130508 or ENSG00000167419	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000117592 encodes a thiol-specific peroxidase, which catalyzes the reduction of hydrogen peroxide and organic hydroperoxides, exhibiting activities such as phospholipase, peroxidase, and acyltransferase. However, the provided reaction, which converts phenylalanine to 2-phenylacetamide, CO2, and H2O, does not involve peroxidase or any related enzymatic activity described for gene ENSG00000117592.	No
ENSG00000135702	Q9GZS9	MAR06829: 3,3-diiodo-L-thyronine + PAPS --> 3,3-diiodo-L-thyronine-4-O-sulfate + H+ + PAP	ENSG00000070614 or ENSG00000111962 or ENSG00000115526 or ENSG00000124302 or ENSG00000130540 or ENSG00000135702 or ENSG00000136720 or ENSG00000138653 or ENSG00000140835 or ENSG00000147119 or ENSG00000153936 or ENSG00000154080 or ENSG00000154252 or ENSG00000164100 or ENSG00000166507 or ENSG00000171004 or ENSG00000173597 or ENSG00000175040 or ENSG00000175229 or ENSG00000183196 or ENSG00000185352 or ENSG00000197093 or ENSG00000198075 or ENSG00000198203	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000135702 encodes a sulfotransferase that specifically transfers sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. It does not transfer sulfate to longer carbohydrate substrates or keratan, and is not involved in generating HEV-expressed ligands for SELL. In contrast, the reaction provided involves the sulfation of 3,3-diiodo-L-thyronine by PAPS to form 3,3-diiodo-L-thyronine-4-O-sulfate. Since the enzyme¡¯s function does not include sulfation of 3,3-diiodo-L-thyronine, the gene is not relevant to the reaction provided.	No
ENSG00000171004	Q96MM7	MAR06829: 3,3-diiodo-L-thyronine + PAPS --> 3,3-diiodo-L-thyronine-4-O-sulfate + H+ + PAP	ENSG00000070614 or ENSG00000111962 or ENSG00000115526 or ENSG00000124302 or ENSG00000130540 or ENSG00000135702 or ENSG00000136720 or ENSG00000138653 or ENSG00000140835 or ENSG00000147119 or ENSG00000153936 or ENSG00000154080 or ENSG00000154252 or ENSG00000164100 or ENSG00000166507 or ENSG00000171004 or ENSG00000173597 or ENSG00000175040 or ENSG00000175229 or ENSG00000183196 or ENSG00000185352 or ENSG00000197093 or ENSG00000198075 or ENSG00000198203	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000171004 encodes an enzyme that specifically catalyzes the 6-O-sulfation of the N-sulfoglucosamine residue in heparan sulfate, utilizing 3'-phosphoadenosine 5'-phosphosulfate (PAPS) as a sulfate donor. This enzyme's activity results in the formation of 6-sulfo-alpha-D-glucosaminyl-[heparan sulfate] and adenosine 3',5'-bisphosphate. However, the provided reaction involves the sulfation of 3,3-diiodo-L-thyronine to form 3,3-diiodo-L-thyronine-4-O-sulfate, which is a different substrate and does not involve heparan sulfate or its sulfation by this specific enzyme. Thus, the gene is not relevant to the reaction provided.	No
ENSG00000130508	Q92626	MAR07628: O2 + phenylalanine --> 2-phenylacetamide + CO2 + H2O	ENSG00000117592 or ENSG00000121053 or ENSG00000130508 or ENSG00000167419	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000130508 encodes a protein that primarily catalyzes various oxidation reactions involving bromide, thiocyanate, iodide, and collagen cross-linking. These reactions are integral to processes like ECM-mediated signaling, angiogenesis, and basement membrane integrity. In contrast, the reaction provided involves the conversion of oxygen and phenylalanine into 2-phenylacetamide, CO2, and H2O, which is unrelated to the gene's annotated functions.	No
ENSG00000184254	P47895	MAR06748: 3-methoxy-4-hydroxyphenylglycolaldehyde + H2O + NAD+ --> 2.0 H+ + NADH + vanillylmandelate	ENSG00000006534 or ENSG00000108602 or ENSG00000132746 or ENSG00000184254	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000184254 encodes an enzyme that catalyzes the NAD-dependent oxidation of aldehyde substrates, specifically all-trans-retinal and all-trans-13,14-dihydroretinal, to their corresponding carboxylic acids. The reactions described for this gene involve retinal and dihydroretinal substrates, whereas the provided reaction involves 3-methoxy-4-hydroxyphenylglycolaldehyde converting to vanillylmandelate. Therefore, the substrate specificity and the product of the gene's catalytic activity do not match the provided reaction.	No
ENSG00000196616	P00325	MAR06756: 3,4-dihydroxymandelaldehyde + H+ + NADH <=> 3,4-dihydroxyphenylethyleneglycol + NAD+	ENSG00000147576 or ENSG00000172955 or ENSG00000187758 or ENSG00000196344 or ENSG00000196616 or ENSG00000197894 or ENSG00000198099 or ENSG00000248144	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000196616 catalyzes reactions involving the NAD-dependent oxidation of all-trans-retinol and its derivatives or the NADH-dependent reduction of all-trans-retinal and its derivatives. However, the provided reaction involves the conversion of 3,4-dihydroxymandelaldehyde to 3,4-dihydroxyphenylethyleneglycol, which is not a substrate or product of the gene's known catalytic activities. Therefore, the gene is not relevant to the provided reaction.	No
ENSG00000184254	P47895	MAR06760: 3,4-dihydroxyphenylacetaldehyde + H2O + NAD+ --> 3,4-dihydroxyphenylacetate + 2.0 H+ + NADH	ENSG00000006534 or ENSG00000108602 or ENSG00000132746 or ENSG00000184254	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000184254 catalyzes the oxidation of aldehyde substrates, such as all-trans-retinal, to their corresponding carboxylic acids, while the provided reaction involves the conversion of 3,4-dihydroxyphenylacetaldehyde to 3,4-dihydroxyphenylacetate, which is not mentioned as a substrate or product associated with the gene's functions. Thus, the gene and the reaction are not related.	No
ENSG00000117592	P30041	MAR06808: dopamine + H2O2 --> dopamine-O-quinone + 2.0 H2O	ENSG00000117592 or ENSG00000121053 or ENSG00000130508 or ENSG00000167419	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000117592 encodes for a thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides and has roles in phospholipid metabolism. However, the reaction provided (dopamine + H2O2 --> dopamine-O-quinone + 2.0 H2O) involves the oxidation of dopamine by hydrogen peroxide, which is not within the scope of the catalytic activities associated with the gene's encoded protein. Thus, there is no direct relevance between the gene and the provided reaction.	No
ENSG00000175040	Q9Y4C5	MAR06829: 3,3-diiodo-L-thyronine + PAPS --> 3,3-diiodo-L-thyronine-4-O-sulfate + H+ + PAP	ENSG00000070614 or ENSG00000111962 or ENSG00000115526 or ENSG00000124302 or ENSG00000130540 or ENSG00000135702 or ENSG00000136720 or ENSG00000138653 or ENSG00000140835 or ENSG00000147119 or ENSG00000153936 or ENSG00000154080 or ENSG00000154252 or ENSG00000164100 or ENSG00000166507 or ENSG00000171004 or ENSG00000173597 or ENSG00000175040 or ENSG00000175229 or ENSG00000183196 or ENSG00000185352 or ENSG00000197093 or ENSG00000198075 or ENSG00000198203	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation: The gene ENSG00000175040 encodes a sulfotransferase enzyme that utilizes 3'-phosphoadenylyl sulfate (PAPS) to catalyze the sulfonation of N-acetylglucosamine (GlcNAc) residues within specific glycoconjugates. The annotated catalytic activities mentioned involve the sulfonation of GlcNAc residues in various oligosaccharide contexts. However, the provided reaction involves the sulfonation of 3,3-diiodo-L-thyronine to produce 3,3-diiodo-L-thyronine-4-O-sulfate, a process which is not within the functional scope of the enzyme encoded by ENSG00000175040. Therefore, this gene is not relevant to the specified reaction.	No
ENSG00000175229	Q96A11	MAR06829: 3,3-diiodo-L-thyronine + PAPS --> 3,3-diiodo-L-thyronine-4-O-sulfate + H+ + PAP	ENSG00000070614 or ENSG00000111962 or ENSG00000115526 or ENSG00000124302 or ENSG00000130540 or ENSG00000135702 or ENSG00000136720 or ENSG00000138653 or ENSG00000140835 or ENSG00000147119 or ENSG00000153936 or ENSG00000154080 or ENSG00000154252 or ENSG00000164100 or ENSG00000166507 or ENSG00000171004 or ENSG00000173597 or ENSG00000175040 or ENSG00000175229 or ENSG00000183196 or ENSG00000185352 or ENSG00000197093 or ENSG00000198075 or ENSG00000198203	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000175229 encodes an enzyme that transfers sulfate groups specifically to beta-galactosyl residues in certain glycans. The described function and substrate specificities include sulfating N-glycans and core2-branched O-glycans, particularly targeting Gal-beta-1,4-GlcNAc and its derivatives. However, the reaction described involves the sulfation of 3,3-diiodo-L-thyronine, which is a completely different substrate, unrelated to glycans. Therefore, based on the gene's annotation and substrate specificity, it is not relevant to the provided reaction.	No
ENSG00000151093	Q9NWU1	MAR02781: H2O + phosphatidylinositol-4,5-bisphosphate <=> 1-phosphatidyl-1D-myo-inositol-5-phosphate + Pi	ENSG00000151093	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000151093 is involved in the biosynthesis of lipoic acid and longer chain fatty acids, essential for mitochondrial function, whereas the provided reaction involves hydrolysis of phosphatidylinositol-4,5-bisphosphate to 1-phosphatidyl-1D-myo-inositol-5-phosphate and inorganic phosphate, which is unrelated to the gene's annotated functions and catalytic activities.	No
ENSG00000150867	P48426	MAR03325: 1-phosphatidyl-1D-myo-inositol-3-phosphate + ATP --> ADP + H+ + phosphatidylinositol-3,5-bisphosphate	ENSG00000150867 or ENSG00000166908 or ENSG00000276293	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000150867 encodes for an enzyme that catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) using ATP or GTP. In contrast, the provided reaction involves converting 1-phosphatidyl-1D-myo-inositol-3-phosphate to phosphatidylinositol-3,5-bisphosphate using ATP. Therefore, the gene's function does not match the described reaction, indicating they are not related.	No Yes
ENSG00000158578	P22557	MAR04657: 2-amino-3-oxoadipate + H+ --> 5-aminolevulinate + CO2	ENSG00000023330 or ENSG00000158578 or ENSG00000178773	Pending No, the gene is not relevant to the reaction provided. Analysis and explanation. The gene ENSG00000158578 catalyzes the pyridoxal 5'-phosphate (PLP)-dependent condensation of succinyl-CoA and glycine to form aminolevulinic acid (ALA), with CoA and CO2 as by-products. The provided reaction involves the conversion of 2-amino-3-oxoadipate and H+ to 5-aminolevulinate and CO2, which does not match the catalytic activity and substrates/products associated with the gene ENSG00000158578. Therefore, the gene is not related to the reaction provided.	No Yes

[feiranl]

The assignments of ENSG00000241119 to MAR06828, ENSG00000150867 to MAR03325, and ENSG00000158578 to MAR04657 look correct. Note that mentioned function for ENSG00000158578 is divided into two reactions: MAR03939 and MAR04657, with MAR04657 representing the half reaction. All other assignments should be updated as discussed. Please proceed with these changes, particularly regarding cofactor usage, ensuring that NAD-dependent enzymes do not utilize NADPH.

[JHL-452b]

LGTM. These entries that the AI ​​judged incorrectly are updated in the check column of the list, and would not be removed in future PR.

[JHL-452b]

Fixed by #899