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Error while reading reading 10x Xenium Ranger based "tif" files #3

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archanabhardwaj opened this issue Jul 2, 2024 · 0 comments

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@archanabhardwaj
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Very nice tool. !!

I'm working with Xenium-based 10x data and have outputs from Xenium Ranger that I'm trying to read along with Xenium TIFF files. Here's my current approach:

tenX_output_dir <- "10X_Dataset/Rep1_outs/"
tenX_output_tif <- "10X_Dataset/Rep1_outs/morphology.mip.ome.tif"
spe_10x <- readXenium(tenX_output_dir, tiff_path = tenX_output_tif)

It give me error :

Error in readTIFF(tiff_path, as.is = TRUE): unable to open /10X_Dataset/Rep1_outs/morphology.mip.ome.tif

After further investigation, I found that it requires an "output" TIFF file from BIDCell. In my case, I also want to compare the "marker purity metrics" from the 10x-based segmentation results.

Could you please suggest how I can make the TIFF file compatible with the readXenium function in the CellSPA method?

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