From 85b3e2d8adb9585669c2dfec2ff0d057f87cad43 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 20 Mar 2024 12:21:56 +1300 Subject: [PATCH 01/40] Updated project infra, see changelog --- .editorconfig | 39 +- .github/workflows/test.yml | 35 +- .gitignore | 1 - .pre-commit-config.yaml | 15 + .prettierignore | 1 - CHANGELOG.md | 26 ++ LICENSE | 695 +-------------------------------- conf/base.config | 1 + conf/manifest.config | 2 +- pangene_local => local_pangene | 0 pangene_pfr => pfr_pangene | 4 +- version_check.sh | 8 + 12 files changed, 120 insertions(+), 707 deletions(-) create mode 100644 CHANGELOG.md rename pangene_local => local_pangene (100%) rename pangene_pfr => pfr_pangene (83%) create mode 100755 version_check.sh diff --git a/.editorconfig b/.editorconfig index 2951ad8..24ab7a6 100644 --- a/.editorconfig +++ b/.editorconfig @@ -8,8 +8,41 @@ trim_trailing_whitespace = true indent_size = 4 indent_style = space -[*.{md,yml,yaml,cff}] +[*.{md,yml,yaml,html,css,scss,js,cff}] indent_size = 2 -[*.nf.test] -insert_final_newline = false +# These files are edited and tested upstream in nf-core/modules +[/modules/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset +indent_size = unset + +[/subworkflows/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset +indent_size = unset + +[/assets/email*] +indent_size = unset + +# ignore Readme +[README.md] +indent_style = unset + +# ignore python +[*.{py}] +indent_style = unset + +# ignore perl +[*.{pl,pm}] +indent_size = unset + +# ignore LICENSE +[LICENSE] +indent_size = unset diff --git a/.github/workflows/test.yml b/.github/workflows/test.yml index 4de425f..e1bacb8 100644 --- a/.github/workflows/test.yml +++ b/.github/workflows/test.yml @@ -13,37 +13,22 @@ concurrency: cancel-in-progress: true jobs: - prettier: - runs-on: ubuntu-latest - steps: - - name: Check out repository - uses: actions/checkout@v4 - - - name: Install NodeJS - uses: actions/setup-node@v4 - with: - node-version: "20" - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check . - - editorconfig: + pre-commit: runs-on: ubuntu-latest steps: - uses: actions/checkout@v4 - - uses: actions/setup-node@v4 + - name: Set up Python 3.11 + uses: actions/setup-python@v5 with: - node-version: "20" + python-version: 3.11 + cache: "pip" - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker + - name: Install pre-commit + run: pip install pre-commit - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(git ls-files | grep -v test | grep -v LICENSE) + - name: Run pre-commit + run: pre-commit run --all-files stub-test: runs-on: ubuntu-latest @@ -78,7 +63,7 @@ jobs: confirm-pass: runs-on: ubuntu-latest - needs: [prettier, editorconfig, stub-test] + needs: [pre-commit, stub-test] if: always() steps: - name: All tests ok diff --git a/.gitignore b/.gitignore index 62d31c0..8bdcb04 100644 --- a/.gitignore +++ b/.gitignore @@ -12,5 +12,4 @@ __pycache__ *.stdout *.stderr -.literature pangene-test/ diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index fc52181..bc85d76 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -3,3 +3,18 @@ repos: rev: "v3.1.0" hooks: - id: prettier + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python + rev: "2.7.3" + hooks: + - id: editorconfig-checker + alias: ec + - repo: local + hooks: + - id: version_checks + name: Version checks + language: system + entry: > + ./version_check.sh + always_run: true + fail_fast: true + pass_filenames: false diff --git a/.prettierignore b/.prettierignore index 24a3687..543341f 100644 --- a/.prettierignore +++ b/.prettierignore @@ -15,5 +15,4 @@ __pycache__ *.stdout *.stderr -.literature pangene-test/ diff --git a/CHANGELOG.md b/CHANGELOG.md new file mode 100644 index 0000000..6961753 --- /dev/null +++ b/CHANGELOG.md @@ -0,0 +1,26 @@ +# PlantandFoodResearch/pangene: Changelog + +The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) +and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). + +## 0.2+dev - [20-Mar-2024] + +### `Added` + +1. Added changelog and semantic versioning +2. Changed license to MIT +3. Updated `.editorconfig` +4. Moved .literature to test/ branch +5. Renamed `pangene_local` to `local_pangene` +6. Renamed `pangene_pfr` to `pfr_pangene` +7. Added versioning checking +8. Updated github workflow to use pre-commit instead of prettier and editorconfig check +9. Added central singularity cache dir for pfr config + +### `Fixed` + +### `Dependencies` + +1. 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If not, see . - -Also add information on how to contact you by electronic and paper mail. - - If the program does terminal interaction, make it output a short -notice like this when it starts in an interactive mode: - - Copyright (C) - This program comes with ABSOLUTELY NO WARRANTY; for details type `show w'. - This is free software, and you are welcome to redistribute it - under certain conditions; type `show c' for details. - -The hypothetical commands `show w' and `show c' should show the appropriate -parts of the General Public License. Of course, your program's commands -might be different; for a GUI interface, you would use an "about box". - - You should also get your employer (if you work as a programmer) or school, -if any, to sign a "copyright disclaimer" for the program, if necessary. -For more information on this, and how to apply and follow the GNU GPL, see -. - - The GNU General Public License does not permit incorporating your program -into proprietary programs. If your program is a subroutine library, you -may consider it more useful to permit linking proprietary applications with -the library. If this is what you want to do, use the GNU Lesser General -Public License instead of this License. But first, please read -. +MIT License + +Copyright (c) Usman Rashid, Jason Shiller + +Permission is hereby granted, free of charge, to any person obtaining a copy +of this software and associated documentation files (the "Software"), to deal +in the Software without restriction, including without limitation the rights +to use, copy, modify, merge, publish, distribute, sublicense, and/or sell +copies of the Software, and to permit persons to whom the Software is +furnished to do so, subject to the following conditions: + +The above copyright notice and this permission notice shall be included in all +copies or substantial portions of the Software. + +THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR +IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, +FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE +AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER +LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, +OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE +SOFTWARE. diff --git a/conf/base.config b/conf/base.config index 6b0d419..467a95b 100644 --- a/conf/base.config +++ b/conf/base.config @@ -6,6 +6,7 @@ profiles { apptainer { envWhitelist = 'APPTAINER_BINDPATH,APPTAINER_BIND' + cacheDir = "/workspace/pangene/singularity" } } diff --git a/conf/manifest.config b/conf/manifest.config index 95537cd..d61659f 100644 --- a/conf/manifest.config +++ b/conf/manifest.config @@ -5,6 +5,6 @@ manifest { description = """A NextFlow pipeline for pan-genome annotation""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.4' - version = '0.2' + version = '0.2+dev' doi = '' } diff --git a/pangene_local b/local_pangene similarity index 100% rename from pangene_local rename to local_pangene diff --git a/pangene_pfr b/pfr_pangene similarity index 83% rename from pangene_pfr rename to pfr_pangene index 608798c..cda85f0 100644 --- a/pangene_pfr +++ b/pfr_pangene @@ -6,8 +6,8 @@ #SBATCH --nodes=1 #SBATCH --ntasks=1 #SBATCH --cpus-per-task=1 -#SBATCH --output pangene_pfr.stdout -#SBATCH --error pangene_pfr.stderr +#SBATCH --output pfr_pangene.stdout +#SBATCH --error pfr_pangene.stderr #SBATCH --mem=4G ml apptainer/1.1 diff --git a/version_check.sh b/version_check.sh new file mode 100755 index 0000000..815c086 --- /dev/null +++ b/version_check.sh @@ -0,0 +1,8 @@ +#!/usr/bin/env bash + +config_version=$(sed -n "s/.*version.*= '\(.*\)'.*/\1/p" conf/manifest.config) + +# Check CHANGELOG version + +grep "## $config_version - " CHANGELOG.md >/dev/null \ + || (echo 'Failed to match CHANGELOG version'; exit 1) From 8d9ee7a93ae46d3f59c8917e2c0cd4be3f8da4b5 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 20 Mar 2024 19:05:21 +1300 Subject: [PATCH 02/40] Updated modules and subworkflows --- conf/modules.config | 2 +- modules.json | 42 +- modules/nf-core/cat/cat/main.nf | 11 +- modules/nf-core/cat/cat/tests/main.nf.test | 23 +- modules/nf-core/cat/fastq/environment.yml | 2 +- modules/nf-core/cat/fastq/main.nf | 1 - modules/nf-core/cat/fastq/tests/main.nf.test | 63 +- .../nf-core/cat/fastq/tests/main.nf.test.snap | 185 ++++-- .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- modules/nf-core/fastp/main.nf | 2 +- modules/nf-core/fastp/tests/main.nf.test | 143 +++-- modules/nf-core/fastp/tests/main.nf.test.snap | 263 ++++++++- .../fastp/tests/nextflow.interleaved.config | 5 + ...low.config => nextflow.save_failed.config} | 3 +- modules/nf-core/fastqc/tests/main.nf.test | 104 ++-- .../nf-core/fastqc/tests/main.nf.test.snap | 76 ++- modules/nf-core/gffread/meta.yml | 4 +- modules/nf-core/gffread/tests/main.nf.test | 26 +- .../nf-core/gffread/tests/main.nf.test.snap | 56 +- .../gffread/tests/nextflow-gff3.config | 5 + modules/nf-core/gunzip/tests/main.nf.test | 9 +- modules/nf-core/samtools/cat/environment.yml | 3 +- modules/nf-core/samtools/cat/main.nf | 4 +- .../nf-core/samtools/cat/tests/main.nf.test | 47 +- .../samtools/cat/tests/main.nf.test.snap | 64 +- modules/nf-core/sortmerna/main.nf | 150 ++--- modules/nf-core/sortmerna/meta.yml | 26 +- .../sortmerna/tests/indexing_only.config | 5 + modules/nf-core/sortmerna/tests/main.nf.test | 231 +++++++- .../nf-core/sortmerna/tests/main.nf.test.snap | 333 ++++++++++- .../sortmerna/tests/premade_index.config | 8 + modules/nf-core/star/align/environment.yml | 1 + modules/nf-core/star/align/tests/main.nf.test | 141 ++--- .../star/genomegenerate/environment.yml | 3 +- .../star/genomegenerate/tests/main.nf.test | 40 +- .../genomegenerate/tests/main.nf.test.snap | 80 ++- .../nf-core/umitools/extract/environment.yml | 2 +- modules/nf-core/umitools/extract/main.nf | 4 +- .../umitools/extract/tests/main.nf.test | 12 +- .../umitools/extract/tests/main.nf.test.snap | 8 +- .../umitools/extract/tests/nextflow.config | 2 +- modules/pfr/lai/tests/main.nf.test | 120 ---- modules/pfr/lai/tests/main.nf.test.snap | 10 - modules/pfr/lai/tests/nextflow.config | 10 - modules/pfr/lai/tests/tags.yml | 2 - .../{ => ltrretriever}/lai/environment.yml | 4 +- modules/pfr/{ => ltrretriever}/lai/main.nf | 42 +- modules/pfr/{ => ltrretriever}/lai/meta.yml | 6 +- .../pfr/ltrretriever/lai/tests/main.nf.test | 166 ++++++ .../ltrretriever/lai/tests/main.nf.test.snap | 100 ++++ .../ltrretriever/lai/tests/nextflow.config | 15 + modules/pfr/ltrretriever/lai/tests/tags.yml | 2 + subworkflows/local/preprocess_rnaseq.nf | 3 +- .../fastq_fastqc_umitools_fastp/main.nf | 22 +- .../fastq_fastqc_umitools_fastp/meta.yml | 11 +- .../tests/main.nf.test | 548 +++++++++++++++++- .../tests/main.nf.test.snap | 500 +++++++++++++++- .../tests/nextflow.config | 11 + .../tests/nextflow.save_trimmed.config | 6 + subworkflows/pfr/fasta_edta_lai/main.nf | 141 +++-- subworkflows/pfr/fasta_edta_lai/meta.yml | 4 +- .../pfr/fasta_edta_lai/tests/main.nf.test | 53 +- .../fasta_edta_lai/tests/main.nf.test.snap | 88 ++- .../pfr/fasta_edta_lai/tests/nextflow.config | 5 + 65 files changed, 3165 insertions(+), 899 deletions(-) create mode 100644 modules/nf-core/fastp/tests/nextflow.interleaved.config rename modules/nf-core/fastp/tests/{nextflow.config => nextflow.save_failed.config} (50%) create mode 100644 modules/nf-core/gffread/tests/nextflow-gff3.config create mode 100644 modules/nf-core/sortmerna/tests/indexing_only.config create mode 100644 modules/nf-core/sortmerna/tests/premade_index.config delete mode 100644 modules/pfr/lai/tests/main.nf.test delete mode 100644 modules/pfr/lai/tests/main.nf.test.snap delete mode 100644 modules/pfr/lai/tests/nextflow.config delete mode 100644 modules/pfr/lai/tests/tags.yml rename modules/pfr/{ => ltrretriever}/lai/environment.yml (75%) rename modules/pfr/{ => ltrretriever}/lai/main.nf (54%) rename modules/pfr/{ => ltrretriever}/lai/meta.yml (91%) create mode 100644 modules/pfr/ltrretriever/lai/tests/main.nf.test create mode 100644 modules/pfr/ltrretriever/lai/tests/main.nf.test.snap create mode 100644 modules/pfr/ltrretriever/lai/tests/nextflow.config create mode 100644 modules/pfr/ltrretriever/lai/tests/tags.yml create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/nextflow.config create mode 100644 subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/nextflow.save_trimmed.config create mode 100644 subworkflows/pfr/fasta_edta_lai/tests/nextflow.config diff --git a/conf/modules.config b/conf/modules.config index fc489bf..09e9452 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -177,7 +177,7 @@ if(params.liftoff_xref_annotations) { process { withName: 'CUSTOM_DUMPSOFTWAREVERSIONS' { publishDir = [ - path: params.outdir, + path: "$params.outdir/pipeline_info", pattern: "software_versions.yml", mode: "copy", enabled: true diff --git a/modules.json b/modules.json index 4e8f0a9..361828c 100644 --- a/modules.json +++ b/modules.json @@ -8,21 +8,16 @@ "custom/restoregffids": { "branch": "main", "git_sha": "e9f6bdd634bdbcd52c5568ba82f16176ec06631f", - "installed_by": ["fasta_edta_lai", "modules"] + "installed_by": ["fasta_edta_lai"] }, "custom/shortenfastaids": { "branch": "main", "git_sha": "5e0e41b51d7fc7f68ae43692b6fe19b95d7f3a8c", - "installed_by": ["fasta_edta_lai", "modules"] + "installed_by": ["fasta_edta_lai"] }, "edta/edta": { "branch": "main", "git_sha": "35468dbb1f35eb17a43d7e05544601c7c3f8cd90", - "installed_by": ["fasta_edta_lai", "modules"] - }, - "lai": { - "branch": "main", - "git_sha": "7e6e3cb41362a045c6bb6065903efa0eba246e87", "installed_by": ["fasta_edta_lai"] }, "liftoff": { @@ -30,6 +25,11 @@ "git_sha": "444b35f4e6285115f84d2bfce49fc0e6d8a2754e", "installed_by": ["modules"] }, + "ltrretriever/lai": { + "branch": "main", + "git_sha": "154661d7c1769532ff7b5f11259644ec200dd47d", + "installed_by": ["fasta_edta_lai"] + }, "repeatmodeler/builddatabase": { "branch": "main", "git_sha": "9da0567f685b2772f65290f2bd6d6347671c8310", @@ -46,7 +46,7 @@ "pfr": { "fasta_edta_lai": { "branch": "main", - "git_sha": "5ae026a98da1331433fa4cf5b667c9abdf43e395", + "git_sha": "ba9c14555adf50c193f326dc91f48b770fe9b1ae", "installed_by": ["subworkflows"] } } @@ -73,17 +73,17 @@ "nf-core": { "cat/cat": { "branch": "master", - "git_sha": "d593e8f6b7d1bbbb2acf43a4b9efeeac8d6720f2", + "git_sha": "9437e6053dccf4aafa022bfd6e7e9de67e625af8", "installed_by": ["modules"] }, "cat/fastq": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "0997b47c93c06b49aa7b3fefda87e728312cf2ca", "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", + "git_sha": "de45447d060b8c8b98575bc637a4a575fd0638e1", "installed_by": ["modules"] }, "fastavalidator": { @@ -93,47 +93,47 @@ }, "fastp": { "branch": "master", - "git_sha": "d086322563bdbb08c94bf15a7db58a39ccdb1520", + "git_sha": "95cf5fe0194c7bf5cb0e3027a2eb7e7c89385080", "installed_by": ["fastq_fastqc_umitools_fastp"] }, "fastqc": { "branch": "master", - "git_sha": "617777a807a1770f73deb38c80004bac06807eef", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["fastq_fastqc_umitools_fastp", "modules"] }, "gffread": { "branch": "master", - "git_sha": "a2d6c3082c5c44b4155a3246daff36701ee49af8", + "git_sha": "b1b959609bda44341120aed1766329909f54b8d0", "installed_by": ["modules"] }, "gunzip": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", "installed_by": ["modules"] }, "samtools/cat": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", "installed_by": ["modules"] }, "sortmerna": { "branch": "master", - "git_sha": "ce558e30784469b88a16923ca96d81899d240b42", + "git_sha": "df05c8db5195867c0bc7b92c1788115b66f0d17d", "installed_by": ["modules"] }, "star/align": { "branch": "master", - "git_sha": "a64788f5ad388f1d2ac5bd5f1f3f8fc81476148c", + "git_sha": "a21faa6a3481af92a343a10926f59c189a2c16c9", "installed_by": ["modules"] }, "star/genomegenerate": { "branch": "master", - "git_sha": "d87a6e2156c2099c09280fa70776eaf0a824817a", + "git_sha": "a21faa6a3481af92a343a10926f59c189a2c16c9", "installed_by": ["modules"] }, "umitools/extract": { "branch": "master", - "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", + "git_sha": "d2c5e76f291379f3dd403e48e46ed7e6ba5da744", "installed_by": ["fastq_fastqc_umitools_fastp"] } } @@ -142,7 +142,7 @@ "nf-core": { "fastq_fastqc_umitools_fastp": { "branch": "master", - "git_sha": "3e8b0c1144ccf60b7848efbdc2be285ff20b49ee", + "git_sha": "cabcc0dadf8366aa7a9930066a7b3dd90d9825d5", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/cat/cat/main.nf b/modules/nf-core/cat/cat/main.nf index 970ab76..adbdbd7 100644 --- a/modules/nf-core/cat/cat/main.nf +++ b/modules/nf-core/cat/cat/main.nf @@ -22,6 +22,8 @@ process CAT_CAT { def args2 = task.ext.args2 ?: '' def file_list = files_in.collect { it.toString() } + // choose appropriate concatenation tool depending on input and output format + // | input | output | command1 | command2 | // |-----------|------------|----------|----------| // | gzipped | gzipped | cat | | @@ -30,7 +32,7 @@ process CAT_CAT { // | ungzipped | gzipped | cat | pigz | // Use input file ending as default - prefix = task.ext.prefix ?: "${meta.id}${file_list[0].substring(file_list[0].lastIndexOf('.'))}" + prefix = task.ext.prefix ?: "${meta.id}${getFileSuffix(file_list[0])}" out_zip = prefix.endsWith('.gz') in_zip = file_list[0].endsWith('.gz') command1 = (in_zip && !out_zip) ? 'zcat' : 'cat' @@ -68,3 +70,10 @@ process CAT_CAT { END_VERSIONS """ } + +// for .gz files also include the second to last extension if it is present. E.g., .fasta.gz +def getFileSuffix(filename) { + def match = filename =~ /^.*?((\.\w{1,5})?(\.\w{1,5}\.gz$))/ + return match ? match[0][1] : filename.substring(filename.lastIndexOf('.')) +} + diff --git a/modules/nf-core/cat/cat/tests/main.nf.test b/modules/nf-core/cat/cat/tests/main.nf.test index ed5a4f1..fcee2d1 100644 --- a/modules/nf-core/cat/cat/tests/main.nf.test +++ b/modules/nf-core/cat/cat/tests/main.nf.test @@ -19,8 +19,8 @@ nextflow_process { [ [ id:'genome', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes', checkIfExists: true) ] ] """ @@ -45,8 +45,8 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes', checkIfExists: true) ] ] """ @@ -72,8 +72,8 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gff3.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/alignment/last/contigs.genome.maf.gz', checkIfExists: true) ] ] """ @@ -102,8 +102,8 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_gff3_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['contigs_genome_maf_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.gff3.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/alignment/last/contigs.genome.maf.gz', checkIfExists: true) ] ] """ @@ -131,8 +131,8 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true), - file(params.test_data['sarscov2']['genome']['genome_sizes'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.sizes', checkIfExists: true) ] ] """ @@ -160,7 +160,7 @@ nextflow_process { [ [ id:'test', single_end:true ], [ - file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/sarscov2/genome/genome.fasta', checkIfExists: true) ] ] """ @@ -176,4 +176,3 @@ nextflow_process { } } } - diff --git a/modules/nf-core/cat/fastq/environment.yml b/modules/nf-core/cat/fastq/environment.yml index bff93ad..8c69b12 100644 --- a/modules/nf-core/cat/fastq/environment.yml +++ b/modules/nf-core/cat/fastq/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - conda-forge::sed=4.7 + - conda-forge::coreutils=8.30 diff --git a/modules/nf-core/cat/fastq/main.nf b/modules/nf-core/cat/fastq/main.nf index 3d96378..f132b2a 100644 --- a/modules/nf-core/cat/fastq/main.nf +++ b/modules/nf-core/cat/fastq/main.nf @@ -76,5 +76,4 @@ process CAT_FASTQ { """ } } - } diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test b/modules/nf-core/cat/fastq/tests/main.nf.test index f5f9418..dab2e14 100644 --- a/modules/nf-core/cat/fastq/tests/main.nf.test +++ b/modules/nf-core/cat/fastq/tests/main.nf.test @@ -16,11 +16,11 @@ nextflow_process { } process { """ - input[0] = [ - [ id:'test', single_end:true ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true) ] - ] + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)] + ]) """ } } @@ -28,8 +28,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out.reads).match() }, - { assert path(process.out.versions.get(0)).getText().contains("cat") } + { assert snapshot(process.out).match() } ) } } @@ -42,13 +41,13 @@ nextflow_process { } process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true)] + ]) """ } } @@ -56,8 +55,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out.reads).match() }, - { assert path(process.out.versions.get(0)).getText().contains("cat") } + { assert snapshot(process.out).match() } ) } } @@ -70,11 +68,11 @@ nextflow_process { } process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:true ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)] + ]) """ } } @@ -82,8 +80,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out.reads).match() }, - { assert path(process.out.versions.get(0)).getText().contains("cat") } + { assert snapshot(process.out).match() } ) } } @@ -96,13 +93,13 @@ nextflow_process { } process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true)] + ]) """ } } @@ -110,8 +107,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out.reads).match() }, - { assert path(process.out.versions.get(0)).getText().contains("cat") } + { assert snapshot(process.out).match() } ) } } @@ -124,10 +120,10 @@ nextflow_process { } process { """ - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:true ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true)] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true)] + ]) """ } } @@ -135,8 +131,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out.reads).match() }, - { assert path(process.out.versions.get(0)).getText().contains("cat") } + { assert snapshot(process.out).match() } ) } } diff --git a/modules/nf-core/cat/fastq/tests/main.nf.test.snap b/modules/nf-core/cat/fastq/tests/main.nf.test.snap index ec2342e..43dfe28 100644 --- a/modules/nf-core/cat/fastq/tests/main.nf.test.snap +++ b/modules/nf-core/cat/fastq/tests/main.nf.test.snap @@ -1,78 +1,169 @@ { "test_cat_fastq_single_end": { "content": [ - [ - [ - { - "id": "test", - "single_end": true - }, - "test.merged.fastq.gz:md5,f9cf5e375f7de81a406144a2c70cc64d" + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.merged.fastq.gz:md5,ee314a9bd568d06617171b0c85f508da" + ] + ], + "1": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" + ], + "reads": [ + [ + { + "id": "test", + "single_end": true + }, + "test.merged.fastq.gz:md5,ee314a9bd568d06617171b0c85f508da" + ] + ], + "versions": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" ] - ] + } ], - "timestamp": "2023-10-17T23:19:12.990284837" + "timestamp": "2024-01-17T17:30:39.816981" }, "test_cat_fastq_single_end_same_name": { "content": [ - [ - [ - { - "id": "test", - "single_end": true - }, - "test.merged.fastq.gz:md5,63f817db7a29a03eb538104495556f66" + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22" + ] + ], + "1": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" + ], + "reads": [ + [ + { + "id": "test", + "single_end": true + }, + "test.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22" + ] + ], + "versions": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" ] - ] + } ], - "timestamp": "2023-10-17T23:19:31.554568147" + "timestamp": "2024-01-17T17:32:35.229332" }, "test_cat_fastq_single_end_single_file": { "content": [ - [ - [ - { - "id": "test", - "single_end": true - }, - "test.merged.fastq.gz:md5,e325ef7deb4023447a1f074e285761af" + { + "0": [ + [ + { + "id": "test", + "single_end": true + }, + "test.merged.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "1": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" + ], + "reads": [ + [ + { + "id": "test", + "single_end": true + }, + "test.merged.fastq.gz:md5,4161df271f9bfcd25d5845a1e220dbec" + ] + ], + "versions": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" ] - ] + } ], - "timestamp": "2023-10-17T23:19:49.629360033" + "timestamp": "2024-01-17T17:34:00.058829" }, "test_cat_fastq_paired_end_same_name": { "content": [ - [ - [ - { - "id": "test", - "single_end": false - }, + { + "0": [ [ - "test_1.merged.fastq.gz:md5,63f817db7a29a03eb538104495556f66", - "test_2.merged.fastq.gz:md5,fe9f266f43a6fc3dcab690a18419a56e" + { + "id": "test", + "single_end": false + }, + [ + "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22", + "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5" + ] ] + ], + "1": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" + ], + "reads": [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22", + "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5" + ] + ] + ], + "versions": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" ] - ] + } ], - "timestamp": "2023-10-17T23:19:40.711617539" + "timestamp": "2024-01-17T17:33:33.031555" }, "test_cat_fastq_paired_end": { "content": [ - [ - [ - { - "id": "test", - "single_end": false - }, + { + "0": [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22", + "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5" + ] + ] + ], + "1": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" + ], + "reads": [ [ - "test_1.merged.fastq.gz:md5,f9cf5e375f7de81a406144a2c70cc64d", - "test_2.merged.fastq.gz:md5,77c8e966e130d8c6b6ec9be52fcb2bda" + { + "id": "test", + "single_end": false + }, + [ + "test_1.merged.fastq.gz:md5,3ad9406595fafec8172368f9cd0b6a22", + "test_2.merged.fastq.gz:md5,a52cab0b840c7178b0ea83df1fdbe8d5" + ] ] + ], + "versions": [ + "versions.yml:md5,d42d6e24d67004608495883e00bd501b" ] - ] + } ], - "timestamp": "2023-10-18T07:53:20.923560211" + "timestamp": "2024-01-17T17:32:02.270935" } } \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index 9b3272b..b48ced2 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.19 + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index f218761..105f926 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/fastp/main.nf b/modules/nf-core/fastp/main.nf index 2a3b679..4fc19b7 100644 --- a/modules/nf-core/fastp/main.nf +++ b/modules/nf-core/fastp/main.nf @@ -29,7 +29,7 @@ process FASTP { def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def adapter_list = adapter_fasta ? "--adapter_fasta ${adapter_fasta}" : "" - def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : '' + def fail_fastq = save_trimmed_fail && meta.single_end ? "--failed_out ${prefix}.fail.fastq.gz" : save_trimmed_fail && !meta.single_end ? "--failed_out ${prefix}.paired.fail.fastq.gz --unpaired1 ${prefix}_1.fail.fastq.gz --unpaired2 ${prefix}_2.fail.fastq.gz" : '' // Added soft-links to original fastqs for consistent naming in MultiQC // Use single ended for interleaved. Add --interleaved_in in config. if ( task.ext.args?.contains('--interleaved_in') ) { diff --git a/modules/nf-core/fastp/tests/main.nf.test b/modules/nf-core/fastp/tests/main.nf.test index 17dce8a..6f1f489 100644 --- a/modules/nf-core/fastp/tests/main.nf.test +++ b/modules/nf-core/fastp/tests/main.nf.test @@ -19,11 +19,10 @@ nextflow_process { save_trimmed_fail = false save_merged = false - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:true ], - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] - ] - + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -68,9 +67,9 @@ nextflow_process { process.out.reads_fail.collect { file(it[1]).getName() } + process.out.reads_merged.collect { file(it[1]).getName() } ).sort() - ).match("test_fastp_single_end-for_stub_match") + ).match("test_fastp_single_end-_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_single_end") } ) } } @@ -89,11 +88,10 @@ nextflow_process { save_trimmed_fail = false save_merged = false - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:true ], - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] - ] - + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -118,7 +116,7 @@ nextflow_process { ).sort() ).match("test_fastp_single_end-for_stub_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_single_end_stub") } ) } } @@ -135,12 +133,11 @@ nextflow_process { save_trimmed_fail = false save_merged = false - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] - ] - + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -199,9 +196,9 @@ nextflow_process { process.out.reads_fail.collect { file(it[1]).getName() } + process.out.reads_merged.collect { file(it[1]).getName() } ).sort() - ).match("test_fastp_paired_end-for_stub_match") + ).match("test_fastp_paired_end_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_paired_end") } ) } } @@ -220,12 +217,11 @@ nextflow_process { save_trimmed_fail = false save_merged = false - input[0] = [ + input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] - ] - + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -249,13 +245,14 @@ nextflow_process { ).sort() ).match("test_fastp_paired_end-for_stub_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_paired_end-stub") } ) } } test("fastp test_fastp_interleaved") { - config './nextflow.config' + + config './nextflow.interleaved.config' when { params { outdir = "$outputDir" @@ -266,10 +263,10 @@ nextflow_process { save_trimmed_fail = false save_merged = false - input[0] = [ [ id:'test', single_end:true ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) ] - ] - + input[0] = Channel.of([ + [ id:'test', single_end:true ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -281,7 +278,7 @@ nextflow_process { def html_text = [ "Q20 bases:25.719000 K (93.033098%)", "paired end (151 cycles + 151 cycles)"] def log_text = [ "Q20 bases: 12922(92.9841%)", - "reads passed filter: 198"] + "reads passed filter: 162"] def read_lines = [ "@ERR5069949.2151832 NS500628:121:HK3MMAFX2:2:21208:10793:15304/1", "TCATAAACCAAAGCACTCACAGTGTCAACAATTTCAGCAGGACAACGCCGACAAGTTCCGAGGAACATGTCTGGACCTATAGTTTTCATAAGTCTACACACTGAATTGAAATATTCTGGTTCTAGTGTGCCCTTAGTTAGCAATGTGCGT", "AAAAAAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEAEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEAAEEEEE - { assert path(process.out.reads_fail.get(0).get(1).get(1)).linesGzip.contains(failed_read2_line) } + { assert path(process.out.reads_fail.get(0).get(1).get(2)).linesGzip.contains(failed_read2_line) } } }, { html_text.each { html_part -> @@ -503,7 +500,7 @@ nextflow_process { { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } } }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_paired_end_trim_fail") } ) } } @@ -519,11 +516,11 @@ nextflow_process { adapter_fasta = [] save_trimmed_fail = false save_merged = true - - input[0] = [ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -592,9 +589,9 @@ nextflow_process { process.out.reads_fail.collect { file(it[1]).getName() } + process.out.reads_merged.collect { file(it[1]).getName() } ).sort() - ).match("test_fastp_paired_end_merged-for_stub_match") + ).match("test_fastp_paired_end_merged_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_paired_end_merged") } ) } } @@ -613,10 +610,11 @@ nextflow_process { save_trimmed_fail = false save_merged = true - input[0] = [ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -640,7 +638,7 @@ nextflow_process { ).sort() ).match("test_fastp_paired_end_merged-for_stub_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_paired_end_merged_stub") } ) } } @@ -653,14 +651,15 @@ nextflow_process { } process { """ - adapter_fasta = file("https://github.com/nf-core/test-datasets/raw/modules/data/delete_me/fastp/adapters.fasta", checkIfExists: true) + adapter_fasta = Channel.of([ file(params.modules_testdata_base_path + 'delete_me/fastp/adapters.fasta', checkIfExists: true) ]) save_trimmed_fail = false save_merged = true - input[0] = [ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] - ] + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) input[1] = adapter_fasta input[2] = save_trimmed_fail input[3] = save_merged @@ -719,7 +718,7 @@ nextflow_process { { assert path(process.out.log.get(0).get(1)).getText().contains(log_part) } } }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("versions_paired_end_merged_adapterlist") } ) } } diff --git a/modules/nf-core/fastp/tests/main.nf.test.snap b/modules/nf-core/fastp/tests/main.nf.test.snap index 1b7d241..3e87628 100644 --- a/modules/nf-core/fastp/tests/main.nf.test.snap +++ b/modules/nf-core/fastp/tests/main.nf.test.snap @@ -1,5 +1,23 @@ { - "test_fastp_paired_end-for_stub_match": { + "fastp test_fastp_interleaved_json": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastp.json:md5,b24e0624df5cc0b11cd5ba21b726fb22" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T16:19:15.063001" + }, + "test_fastp_paired_end_merged-for_stub_match": { "content": [ [ [ @@ -9,12 +27,29 @@ "test.fastp.html", "test.fastp.json", "test.fastp.log", + "test.merged.fastq.gz", "{id=test, single_end=false}" ] ], - "timestamp": "2023-12-21T09:44:37.202512" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:10:13.467574" }, - "fastp test_fastp_interleaved_json": { + "versions_interleaved": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:56:24.615634793" + }, + "test_fastp_single_end_json": { "content": [ [ [ @@ -22,13 +57,64 @@ "id": "test", "single_end": true }, - "test.fastp.json:md5,168f516f7bd4b7b6c32da7cba87299a4" + "test.fastp.json:md5,c852d7a6dba5819e4ac8d9673bedcacc" ] ] ], - "timestamp": "2023-10-17T11:04:45.794175881" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T16:18:43.526412" }, - "test_fastp_paired_end_merged-for_stub_match": { + "versions_paired_end": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:55:42.333545689" + }, + "test_fastp_paired_end_match": { + "content": [ + [ + [ + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:03:06.431833729" + }, + "test_fastp_interleaved-_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T16:19:15.111894" + }, + "test_fastp_paired_end_merged_match": { "content": [ [ [ @@ -42,29 +128,102 @@ "{id=test, single_end=false}" ] ], - "timestamp": "2023-12-21T09:53:45.237014" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:08:44.496251446" }, - "test_fastp_single_end_json": { + "versions_single_end_stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:55:27.354051299" + }, + "versions_interleaved-stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:56:46.535528418" + }, + "versions_single_end_trim_fail": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:03.724591407" + }, + "test_fastp_paired_end-for_stub_match": { "content": [ [ [ - { - "id": "test", - "single_end": true - }, - "test.fastp.json:md5,c852d7a6dba5819e4ac8d9673bedcacc" - ] + "test_1.fastp.fastq.gz", + "test_2.fastp.fastq.gz" + ], + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=false}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:07:15.398827" + }, + "versions_paired_end-stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" ] ], - "timestamp": "2023-10-17T11:04:10.566343705" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:56:06.50017282" }, - "versions": { + "versions_single_end": { "content": [ [ "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" ] ], - "timestamp": "2023-10-17T11:04:10.582076024" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:55:07.67921647" + }, + "versions_paired_end_merged_stub": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:47.350653154" }, "test_fastp_interleaved-for_stub_match": { "content": [ @@ -76,7 +235,23 @@ "{id=test, single_end=true}" ] ], - "timestamp": "2023-12-21T09:48:43.148485" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:08:06.127974" + }, + "versions_paired_end_trim_fail": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:18.140484878" }, "test_fastp_single_end-for_stub_match": { "content": [ @@ -88,7 +263,51 @@ "{id=test, single_end=true}" ] ], - "timestamp": "2023-12-21T09:20:07.254788" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:06:00.244202" + }, + "test_fastp_single_end-_match": { + "content": [ + [ + "test.fastp.fastq.gz", + "test.fastp.html", + "test.fastp.json", + "test.fastp.log", + "{id=test, single_end=true}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T16:18:43.580336" + }, + "versions_paired_end_merged_adapterlist": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:05:37.845370554" + }, + "versions_paired_end_merged": { + "content": [ + [ + "versions.yml:md5,48ffc994212fb1fc9f83a74fa69c9f02" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T11:59:32.860543858" }, "test_fastp_single_end_trim_fail_json": { "content": [ @@ -102,6 +321,10 @@ ] ] ], - "timestamp": "2023-10-17T11:05:00.379878948" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-17T18:08:41.942317" } } \ No newline at end of file diff --git a/modules/nf-core/fastp/tests/nextflow.interleaved.config b/modules/nf-core/fastp/tests/nextflow.interleaved.config new file mode 100644 index 0000000..4be8dbd --- /dev/null +++ b/modules/nf-core/fastp/tests/nextflow.interleaved.config @@ -0,0 +1,5 @@ +process { + withName: FASTP { + ext.args = "--interleaved_in -e 30" + } +} diff --git a/modules/nf-core/fastp/tests/nextflow.config b/modules/nf-core/fastp/tests/nextflow.save_failed.config similarity index 50% rename from modules/nf-core/fastp/tests/nextflow.config rename to modules/nf-core/fastp/tests/nextflow.save_failed.config index 0f7849a..53b61b0 100644 --- a/modules/nf-core/fastp/tests/nextflow.config +++ b/modules/nf-core/fastp/tests/nextflow.save_failed.config @@ -1,6 +1,5 @@ process { - withName: FASTP { - ext.args = "--interleaved_in" + ext.args = "-e 30" } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index ad9bc54..70edae4 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -13,12 +13,10 @@ nextflow_process { when { process { """ - input[0] = [ - [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) """ } } @@ -35,7 +33,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_single") } ) } } @@ -44,15 +42,13 @@ nextflow_process { when { process { - """ - input[0] = [ - [id: 'test', single_end: false], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) - ] - ] - """ + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ } } @@ -67,7 +63,7 @@ nextflow_process { { assert path(process.out.html[0][1][0]).text.contains("File typeConventional base calls") }, { assert path(process.out.html[0][1][1]).text.contains("File typeConventional base calls") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } ) } } @@ -76,11 +72,11 @@ nextflow_process { when { process { - """ - input[0] = [ - [id: 'test', single_end: false], // meta map - file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) - ] + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) """ } } @@ -93,7 +89,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } @@ -102,12 +98,12 @@ nextflow_process { when { process { - """ - input[0] = [ - [id: 'test', single_end: false], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) - ] - """ + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ } } @@ -119,7 +115,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } ) } } @@ -128,17 +124,15 @@ nextflow_process { when { process { - """ - input[0] = [ - [id: 'test', single_end: false], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) - ] - ] - """ + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ } } @@ -159,7 +153,7 @@ nextflow_process { { assert path(process.out.html[0][1][2]).text.contains("File typeConventional base calls") }, { assert path(process.out.html[0][1][3]).text.contains("File typeConventional base calls") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } ) } } @@ -168,12 +162,12 @@ nextflow_process { when { process { - """ - input[0] = [ - [ id:'mysample', single_end:true ], // meta map - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - """ + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ } } @@ -185,7 +179,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("File typeConventional base calls") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } ) } } @@ -197,12 +191,10 @@ nextflow_process { when { process { """ - input[0] = [ - [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) """ } } @@ -212,7 +204,7 @@ nextflow_process { { assert process.success }, { assert snapshot(process.out.html.collect { file(it[1]).getName() } + process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } + process.out.versions ).match("fastqc_stub") } ) } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 5ef5afb..86f7c31 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,5 +1,17 @@ { - "sarscov2 single-end [fastq] - stub": { + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { "content": [ [ "test.html", @@ -7,14 +19,70 @@ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2023-12-29T02:48:05.126117287" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" }, - "versions": { + "fastqc_versions_custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2023-12-29T02:46:49.507942667" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/gffread/meta.yml b/modules/nf-core/gffread/meta.yml index 27ac310..d38cbcd 100644 --- a/modules/nf-core/gffread/meta.yml +++ b/modules/nf-core/gffread/meta.yml @@ -31,6 +31,6 @@ output: description: File containing software versions pattern: "versions.yml" authors: - - "@emiller88" + - "@edmundmiller" maintainers: - - "@emiller88" + - "@edmundmiller" diff --git a/modules/nf-core/gffread/tests/main.nf.test b/modules/nf-core/gffread/tests/main.nf.test index 3c064b3..452aba1 100644 --- a/modules/nf-core/gffread/tests/main.nf.test +++ b/modules/nf-core/gffread/tests/main.nf.test @@ -18,17 +18,19 @@ nextflow_process { } process { """ - input[0] = file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + input[0] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) """ } } then { assertAll ( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert process.out.gtf != null }, - { assert process.out.gffread_gff == [] } + { assert process.success }, + { assert snapshot( + process.out.gtf, + process.out.versions + ).match() }, + { assert process.out.gffread_gff == [] } ) } @@ -36,23 +38,27 @@ nextflow_process { test("sarscov2-gff3-gff3") { + config "./nextflow-gff3.config" + when { params { outdir = "$outputDir" } process { """ - input[0] = file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + input[0] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) """ } } then { assertAll ( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert process.out.gtf == [] }, - { assert process.out.gffread_gff != null }, + { assert process.success }, + { assert snapshot( + process.out.gffread_gff, + process.out.versions + ).match() }, + { assert process.out.gtf == [] }, ) } diff --git a/modules/nf-core/gffread/tests/main.nf.test.snap b/modules/nf-core/gffread/tests/main.nf.test.snap index 1f1342e..00a11a4 100644 --- a/modules/nf-core/gffread/tests/main.nf.test.snap +++ b/modules/nf-core/gffread/tests/main.nf.test.snap @@ -1,52 +1,24 @@ { "sarscov2-gff3-gtf": { "content": [ - { - "0": [ - "genome.gtf:md5,2394072d7d31530dfd590c4a117bf6e3" - ], - "1": [ - - ], - "2": [ - "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" - ], - "gffread_gff": [ - - ], - "gtf": [ - "genome.gtf:md5,2394072d7d31530dfd590c4a117bf6e3" - ], - "versions": [ - "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" - ] - } + [ + "genome.gtf:md5,2394072d7d31530dfd590c4a117bf6e3" + ], + [ + "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" + ] ], - "timestamp": "2023-11-29T15:39:30.006985" + "timestamp": "2024-01-23T20:00:32.688779117" }, "sarscov2-gff3-gff3": { "content": [ - { - "0": [ - - ], - "1": [ - "genome.gffread.gff3:md5,a7d40d99dcddac23ac673c473279ea2d" - ], - "2": [ - "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" - ], - "gffread_gff": [ - "genome.gffread.gff3:md5,a7d40d99dcddac23ac673c473279ea2d" - ], - "gtf": [ - - ], - "versions": [ - "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" - ] - } + [ + "genome.gffread.gff3:md5,a7d40d99dcddac23ac673c473279ea2d" + ], + [ + "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" + ] ], - "timestamp": "2023-11-29T15:39:34.636061" + "timestamp": "2024-01-23T20:07:11.457356625" } } \ No newline at end of file diff --git a/modules/nf-core/gffread/tests/nextflow-gff3.config b/modules/nf-core/gffread/tests/nextflow-gff3.config new file mode 100644 index 0000000..afe0830 --- /dev/null +++ b/modules/nf-core/gffread/tests/nextflow-gff3.config @@ -0,0 +1,5 @@ +process { + withName: GFFREAD { + ext.args = '' + } +} diff --git a/modules/nf-core/gunzip/tests/main.nf.test b/modules/nf-core/gunzip/tests/main.nf.test index d031792..6406008 100644 --- a/modules/nf-core/gunzip/tests/main.nf.test +++ b/modules/nf-core/gunzip/tests/main.nf.test @@ -15,10 +15,11 @@ nextflow_process { } process { """ - input[0] = [ - [], - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] + input[0] = Channel.of([ + [], + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ] + ) """ } } diff --git a/modules/nf-core/samtools/cat/environment.yml b/modules/nf-core/samtools/cat/environment.yml index 0455a7d..75f10f7 100644 --- a/modules/nf-core/samtools/cat/environment.yml +++ b/modules/nf-core/samtools/cat/environment.yml @@ -4,4 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::samtools=1.18 + - bioconda::samtools=1.19.2 + - bioconda::htslib=1.19.1 diff --git a/modules/nf-core/samtools/cat/main.nf b/modules/nf-core/samtools/cat/main.nf index b3b2508..06615aa 100644 --- a/modules/nf-core/samtools/cat/main.nf +++ b/modules/nf-core/samtools/cat/main.nf @@ -4,8 +4,8 @@ process SAMTOOLS_CAT { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/samtools:1.18--h50ea8bc_1' : - 'biocontainers/samtools:1.18--h50ea8bc_1' }" + 'https://depot.galaxyproject.org/singularity/samtools:1.19.2--h50ea8bc_0' : + 'biocontainers/samtools:1.19.2--h50ea8bc_0' }" input: tuple val(meta), path(input_files, stageAs: "?/*") diff --git a/modules/nf-core/samtools/cat/tests/main.nf.test b/modules/nf-core/samtools/cat/tests/main.nf.test index 49c633f..dad80b8 100644 --- a/modules/nf-core/samtools/cat/tests/main.nf.test +++ b/modules/nf-core/samtools/cat/tests/main.nf.test @@ -9,18 +9,16 @@ nextflow_process { tag "samtools" tag "samtools/cat" - test("sarscov2 - [bam1, bam2]") { + test("bams") { when { process { """ - input[0] = [ - [ id:'test', single_end:false ], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_unaligned_bam'], checkIfExists: true) - ] - ] + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.unaligned.bam', checkIfExists: true) ] + ]) """ } } @@ -28,30 +26,25 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.cram, - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("bams_bam") }, + { assert snapshot(process.out.cram).match("bams_cram") }, + { assert snapshot(process.out.versions).match("bams_versions") } ) } - } - test("sarscov2 - [bam1, bam2] - stub") { + test("bams_stub") { options "-stub" when { process { """ - input[0] = [ - [ id:'test', single_end:false ], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_paired_end_bam'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_unaligned_bam'], checkIfExists: true) - ] - ] + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.bam', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.unaligned.bam', checkIfExists: true) ] + ]) """ } } @@ -59,14 +52,10 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot( - file(process.out.bam[0][1]).name, - process.out.cram, - process.out.versions - ).match() } + { assert snapshot(file(process.out.bam[0][1]).name).match("bams_stub_bam") }, + { assert snapshot(process.out.cram).match("bams_stub_cram") }, + { assert snapshot(process.out.versions).match("bams_stub_versions") } ) } - } - } diff --git a/modules/nf-core/samtools/cat/tests/main.nf.test.snap b/modules/nf-core/samtools/cat/tests/main.nf.test.snap index 298e25d..f99cdd6 100644 --- a/modules/nf-core/samtools/cat/tests/main.nf.test.snap +++ b/modules/nf-core/samtools/cat/tests/main.nf.test.snap @@ -1,26 +1,70 @@ { - "sarscov2 - [bam1, bam2]": { + "bams_stub_cram": { "content": [ - "test.bam", [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-02T16:45:42.587418" + }, + "bams_stub_versions": { + "content": [ [ - "versions.yml:md5,f10a4f6b2e0272bef2ceb4ca826a15a1" + "versions.yml:md5,e214a92343158372aa79dabe0fb0064a" ] ], - "timestamp": "2023-12-04T14:00:18.264348819" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:15:40.92408626" + }, + "bams_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-02T16:45:37.965199" }, - "sarscov2 - [bam1, bam2] - stub": { + "bams_cram": { "content": [ - "test.bam", [ - ], + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-02T16:45:37.96805" + }, + "bams_stub_bam": { + "content": [ + "test.bam" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-02T16:45:42.583881" + }, + "bams_versions": { + "content": [ [ - "versions.yml:md5,f10a4f6b2e0272bef2ceb4ca826a15a1" + "versions.yml:md5,e214a92343158372aa79dabe0fb0064a" ] ], - "timestamp": "2023-12-04T14:03:17.714482742" + "meta": { + "nf-test": "0.8.4", + "nextflow": "24.01.0" + }, + "timestamp": "2024-02-13T16:15:33.224336325" } } \ No newline at end of file diff --git a/modules/nf-core/sortmerna/main.nf b/modules/nf-core/sortmerna/main.nf index 29c640c..7c17e50 100644 --- a/modules/nf-core/sortmerna/main.nf +++ b/modules/nf-core/sortmerna/main.nf @@ -9,88 +9,102 @@ process SORTMERNA { input: tuple val(meta), path(reads) - path fastas + tuple val(meta2), path(fastas) + tuple val(meta3), path(index) output: - tuple val(meta), path("*non_rRNA.fastq.gz"), emit: reads - tuple val(meta), path("*.log") , emit: log - path "versions.yml" , emit: versions + tuple val(meta), path("*non_rRNA.fastq.gz"), emit: reads, optional: true + tuple val(meta), path("*.log") , emit: log, optional: true + tuple val(meta2), path("idx") , emit: index, optional: true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - if (meta.single_end) { - """ - sortmerna \\ - ${'--ref '+fastas.join(' --ref ')} \\ - --reads $reads \\ - --threads $task.cpus \\ - --workdir . \\ - --aligned rRNA_reads \\ - --fastx \\ - --other non_rRNA_reads \\ - $args + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" - mv non_rRNA_reads.f*q.gz ${prefix}.non_rRNA.fastq.gz - mv rRNA_reads.log ${prefix}.sortmerna.log + def index_only = args.contains('--index 1')? true : false + def skip_index = args.contains('--index 0')? true : false + def paired_end = reads instanceof List + def paired_cmd = '' + def reads_args = '' + def out2_cmd = '' + def mv_cmd = '' + def reads_input = '' + def refs_input = '' - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sortmerna: \$(echo \$(sortmerna --version 2>&1) | sed 's/^.*SortMeRNA version //; s/ Build Date.*\$//') - END_VERSIONS - """ - } else { - """ - sortmerna \\ - ${'--ref '+fastas.join(' --ref ')} \\ - --reads ${reads[0]} \\ - --reads ${reads[1]} \\ - --threads $task.cpus \\ - --workdir . \\ - --aligned rRNA_reads \\ - --fastx \\ - --other non_rRNA_reads \\ - --paired_in \\ - --out2 \\ - $args + if (! index_only){ + reads_args = '--aligned rRNA_reads --fastx --other non_rRNA_reads' + reads_input = paired_end ? reads.collect{"--reads $it"}.join(' ') : "--reads $reads" + def n_fastq = paired_end ? reads.size() : 1 + if ( n_fastq == 1 ) { + mv_cmd = """ + mv non_rRNA_reads.f*q.gz ${prefix}.non_rRNA.fastq.gz + mv rRNA_reads.log ${prefix}.sortmerna.log + """ + } else { + mv_cmd = """ + mv non_rRNA_reads_fwd.f*q.gz ${prefix}_1.non_rRNA.fastq.gz + mv non_rRNA_reads_rev.f*q.gz ${prefix}_2.non_rRNA.fastq.gz + mv rRNA_reads.log ${prefix}.sortmerna.log + """ + paired_cmd = "--paired_in" + out2_cmd = "--out2" + } + } + """ + sortmerna \\ + ${'--ref '+fastas.join(' --ref ')} \\ + $refs_input \\ + $reads_input \\ + --threads $task.cpus \\ + --workdir . \\ + $reads_args \\ + $paired_cmd \\ + $out2_cmd \\ + $args - mv non_rRNA_reads_fwd.f*q.gz ${prefix}_1.non_rRNA.fastq.gz - mv non_rRNA_reads_rev.f*q.gz ${prefix}_2.non_rRNA.fastq.gz - mv rRNA_reads.log ${prefix}.sortmerna.log + $mv_cmd - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sortmerna: \$(echo \$(sortmerna --version 2>&1) | sed 's/^.*SortMeRNA version //; s/ Build Date.*\$//') - END_VERSIONS - """ - } + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sortmerna: \$(echo \$(sortmerna --version 2>&1) | sed 's/^.*SortMeRNA version //; s/ Build Date.*\$//') + END_VERSIONS + """ stub: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" - if (meta.single_end) { - """ - touch ${prefix}.non_rRNA.fastq.gz - touch ${prefix}.sortmerna.log + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sortmerna: \$(echo \$(sortmerna --version 2>&1) | sed 's/^.*SortMeRNA version //; s/ Build Date.*\$//') - END_VERSIONS - """ - } else { - """ - touch ${prefix}_1.non_rRNA.fastq.gz - touch ${prefix}_2.non_rRNA.fastq.gz - touch ${prefix}.sortmerna.log + def index_only = args.contains('--index 1')? true : false + def paired_end = reads instanceof List + def paired_cmd = '' + def out2_cmd = '' + def mv_cmd = '' + def reads_input = '' - cat <<-END_VERSIONS > versions.yml - "${task.process}": - sortmerna: \$(echo \$(sortmerna --version 2>&1) | sed 's/^.*SortMeRNA version //; s/ Build Date.*\$//') - END_VERSIONS - """ + if (! index_only){ + reads_input = paired_end ? reads.collect{"--reads $it"}.join(' ') : "--reads $reads" + def n_fastq = paired_end ? reads.size() : 1 + if ( n_fastq == 1 ) { + mv_cmd = "touch ${prefix}.non_rRNA.fastq.gz" + } else { + mv_cmd = """ + touch ${prefix}_1.non_rRNA.fastq.gz + touch ${prefix}_2.non_rRNA.fastq.gz + """ + } } + """ + $mv_cmd + mkdir -p idx + touch ${prefix}.sortmerna.log + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + sortmerna: \$(echo \$(sortmerna --version 2>&1) | sed 's/^.*SortMeRNA version //; s/ Build Date.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/sortmerna/meta.yml b/modules/nf-core/sortmerna/meta.yml index de0b18e..c0a2a58 100644 --- a/modules/nf-core/sortmerna/meta.yml +++ b/modules/nf-core/sortmerna/meta.yml @@ -23,16 +23,31 @@ input: description: | List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively. + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test' ] - fastas: type: file description: | Path to reference file(s) + - meta3: + type: map + description: | + Groovy Map containing index information + e.g. [ id:'test' ] + - index: + type: directory + description: | + Path to index directory of a previous sortmerna run output: - meta: type: map description: | Groovy Map containing sample information - e.g. [ id:'test', single_end:false ] + e.g. [ id:'test', single_end:false ], or reference information from an + indexing-only run - reads: type: file description: The filtered fastq reads @@ -41,6 +56,15 @@ output: type: file description: SortMeRNA log file pattern: "*sortmerna.log" + - meta2: + type: map + description: | + Groovy Map containing reference information + e.g. [ id:'test' ] + - index: + type: directory + description: | + Path to index directory generated by sortmern - versions: type: file description: File containing software versions diff --git a/modules/nf-core/sortmerna/tests/indexing_only.config b/modules/nf-core/sortmerna/tests/indexing_only.config new file mode 100644 index 0000000..3e74a32 --- /dev/null +++ b/modules/nf-core/sortmerna/tests/indexing_only.config @@ -0,0 +1,5 @@ +process { + withName: 'SORTMERNA' { + ext.args = '--index 1' + } +} diff --git a/modules/nf-core/sortmerna/tests/main.nf.test b/modules/nf-core/sortmerna/tests/main.nf.test index 8a01e2a..73bc119 100644 --- a/modules/nf-core/sortmerna/tests/main.nf.test +++ b/modules/nf-core/sortmerna/tests/main.nf.test @@ -7,15 +7,73 @@ nextflow_process { tag "modules_nfcore" tag "sortmerna" + test("sarscov2 indexing only") { + + config './indexing_only.config' + + when { + process { + """ + input[0] = Channel.of([[],[]]) + input[1] = [ [id:'test2'], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ] + ] + input[2] = Channel.of([[],[]]) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert ! process.out.reads }, + { assert snapshot(process.out.index).match("index_index_only") }, + { assert snapshot(process.out.versions).match("versions_index_only") } + ) + } + + } + + test("sarscov2 indexing only stub") { + + options '-stub' + config './indexing_only.config' + + when { + process { + """ + input[0] = Channel.of([[],[]]) + input[1] = [ [id:'test2'], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ] + ] + input[2] = Channel.of([[],[]]) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert ! process.out.reads }, + { assert snapshot(process.out.index).match("index_only_stub") }, + { assert snapshot(process.out.versions).match("versions_index_only_stub") } + ) + } + + } + test("sarscov2 single_end") { when { process { """ - input[0] = [ [ id:'test', single_end:true ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] - ] - input[1] = [ file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + input[0] = [ [ id:'test' ], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true) ] + ] + input[1] = [ [id:'test2'], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ] + ] + input[2] = Channel.of([[],[]]) """ } } @@ -32,9 +90,10 @@ nextflow_process { process.out.reads.collect { file(it[1]).getName() } + process.out.log.collect { file(it[1]).getName() } ).sort() - ).match("sarscov2 single_end-for_stub_match") + ).match("sarscov2 single_end_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.index).match("index_single_end") }, + { assert snapshot(process.out.versions).match("versions_single_end") } ) } @@ -47,10 +106,13 @@ nextflow_process { when { process { """ - input[0] = [ [ id:'test', single_end:true ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] - ] - input[1] = [ file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + input[0] = [ [ id:'test' ], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true) ] + ] + input[1] = [ [id:'test2'], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ] + ] + input[2] = Channel.of([[],[]]) """ } } @@ -67,7 +129,8 @@ nextflow_process { ).sort() ).match("sarscov2 single_end-for_stub_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.index).match("index_single_end_stub") }, + { assert snapshot(process.out.versions).match("versions_single_end_stub") } ) } @@ -78,11 +141,17 @@ nextflow_process { when { process { """ - input[0] = [ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] - ] - input[1] = [ file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + input[0] = [ + [ id:'test' ], // meta map + [ + file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true), + file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_2.fastq.gz", checkIfExists: true) + ] + ] + input[1] = [ [id:'test2'], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ] + ] + input[2] = Channel.of([[],[]]) """ } } @@ -99,9 +168,10 @@ nextflow_process { process.out.reads.collect { it[1].collect { item -> file(item).getName() } } + process.out.log.collect { file(it[1]).getName() } ).sort() - ).match("sarscov2 paired_end-for_stub_match") + ).match("sarscov2 paired_end_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.index).match("index_paired_end") }, + { assert snapshot(process.out.versions).match("versions_paired_end") } ) } @@ -114,11 +184,16 @@ nextflow_process { when { process { """ - input[0] = [ [ id:'test', single_end:false ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) ] - ] - input[1] = [ file(params.test_data['sarscov2']['genome']['genome_fasta'], checkIfExists: true) ] + input[0] = [ [ id:'test' ], // meta map + [ + file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true), + file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_2.fastq.gz", checkIfExists: true) + ] + ] + input[1] = [ [id:'test2'], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ] + ] + input[2] = Channel.of([[],[]]) """ } } @@ -135,10 +210,118 @@ nextflow_process { ).sort() ).match("sarscov2 paired_end-for_stub_match") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.index).match("index_paired_end_stub") }, + { assert snapshot(process.out.versions).match("versions_paired_end_stub") } ) } } + test("sarscov2 single_end premade_index") { + + config './premade_index.config' + + setup { + + run("SORTMERNA", alias: "SORTMERNA_INDEX") { + script "../main.nf" + process { + """ + input[0] = Channel.of([[],[]]) + input[1] = [ [id:'test2'], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ] + ] + input[2] = Channel.of([[],[]]) + """ + } + } + } + + when { + process { + """ + input[0] = [ [ id:'test' ], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true) ] + ] + input[1] = [ [id:'test2'], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ] + ] + input[2] = SORTMERNA_INDEX.out.index + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.reads }, + { assert file(process.out.log[0][1]).text.contains("Total reads passing E-value threshold = 100 (100.00)") }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + ).sort() + ).match("sarscov2 single_end_premade_index_match") + }, + { assert snapshot(process.out.index).match("index_single_end_premade_index") }, + { assert snapshot(process.out.versions).match("versions_single_end_premade_index") } + ) + } + } + + test("sarscov2 single_end premade_index stub") { + + config './premade_index.config' + options '-stub' + + setup { + + run("SORTMERNA", alias: "SORTMERNA_INDEX") { + script "../main.nf" + process { + """ + input[0] = Channel.of([[],[]]) + input[1] = [ [id:'test2'], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ] + ] + input[2] = Channel.of([[],[]]) + """ + } + } + } + + when { + process { + """ + input[0] = [ [ id:'test' ], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true) ] + ] + input[1] = [ [id:'test2'], // meta map + [ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ] + ] + input[2] = SORTMERNA_INDEX.out.index + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert process.out.reads }, + { + assert snapshot( + ( + [process.out.reads[0][0].toString()] + // meta + process.out.reads.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + ).sort() + ).match("sarscov2 single_end_premade_index_match_stub") + }, + { assert snapshot(process.out.index).match("index_single_end_premade_index_stub") }, + { assert snapshot(process.out.versions).match("versions_single_end_premade_index_stub") } + ) + } + } } diff --git a/modules/nf-core/sortmerna/tests/main.nf.test.snap b/modules/nf-core/sortmerna/tests/main.nf.test.snap index e502000..86e8473 100644 --- a/modules/nf-core/sortmerna/tests/main.nf.test.snap +++ b/modules/nf-core/sortmerna/tests/main.nf.test.snap @@ -1,33 +1,352 @@ { - "sarscov2 single_end-for_stub_match": { + "versions_paired_end_stub": { + "content": [ + [ + "versions.yml:md5,7df9d50209f351e1f75e05a1fad6ba4b" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-07T12:27:11.223149" + }, + "index_paired_end_stub": { + "content": [ + [ + [ + { + "id": "test2" + }, + [ + + ] + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T14:24:25.384097178" + }, + "versions_paired_end": { + "content": [ + [ + "versions.yml:md5,7df9d50209f351e1f75e05a1fad6ba4b" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-07T12:27:04.517155" + }, + "versions_single_end_stub": { + "content": [ + [ + "versions.yml:md5,7df9d50209f351e1f75e05a1fad6ba4b" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-01T12:10:35.228450189" + }, + "sarscov2 single_end_match": { "content": [ [ "test.non_rRNA.fastq.gz", "test.sortmerna.log", - "{id=test, single_end=true}" + "{id=test}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-07T12:28:23.20327" + }, + "index_only_stub": { + "content": [ + [ + [ + { + "id": "test2" + }, + [ + + ] + ] ] ], - "timestamp": "2023-12-21T11:56:00.15356" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T15:00:47.128504164" }, - "versions": { + "index_single_end_premade_index": { + "content": [ + [ + [ + { + "id": "test2" + }, + [ + "2415186086593376314.bursttrie_0.dat:md5,74f7f020e8d46e24a8a2e9c5fbcd564a", + "2415186086593376314.kmer_0.dat:md5,4a0bcb71b120f6a6949b7969292ef2e7", + "2415186086593376314.pos_0.dat:md5,bc2875e4cc4017707306565e396839ef", + "2415186086593376314.stats:md5,67c9d4c768f28a450fc82a2b5d43db5c" + ] + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T15:01:53.832643452" + }, + "versions_single_end_premade_index": { "content": [ [ "versions.yml:md5,7df9d50209f351e1f75e05a1fad6ba4b" ] ], - "timestamp": "2023-12-21T11:56:00.200244" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T15:01:53.902154982" }, "sarscov2 paired_end-for_stub_match": { "content": [ [ + "{id=test}", [ "test_1.non_rRNA.fastq.gz", "test_2.non_rRNA.fastq.gz" ], + "test.sortmerna.log" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-07T12:28:56.063579" + }, + "index_paired_end": { + "content": [ + [ + [ + { + "id": "test2" + }, + [ + "2415186086593376314.bursttrie_0.dat:md5,74f7f020e8d46e24a8a2e9c5fbcd564a", + "2415186086593376314.kmer_0.dat:md5,4a0bcb71b120f6a6949b7969292ef2e7", + "2415186086593376314.pos_0.dat:md5,bc2875e4cc4017707306565e396839ef", + "2415186086593376314.stats:md5,67c9d4c768f28a450fc82a2b5d43db5c" + ] + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T14:24:14.272659781" + }, + "sarscov2 single_end_premade_index_match_stub": { + "content": [ + [ + "test.non_rRNA.fastq.gz", + "test.sortmerna.log", + "{id=test}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T15:05:01.913287272" + }, + "sarscov2 single_end-for_stub_match": { + "content": [ + [ + "test.non_rRNA.fastq.gz", + "test.sortmerna.log", + "{id=test}" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-07T12:28:29.197913" + }, + "sarscov2 paired_end_match": { + "content": [ + [ + "{id=test}", + [ + "test_1.non_rRNA.fastq.gz", + "test_2.non_rRNA.fastq.gz" + ], + "test.sortmerna.log" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-07T12:28:49.914992" + }, + "versions_single_end": { + "content": [ + [ + "versions.yml:md5,7df9d50209f351e1f75e05a1fad6ba4b" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T14:36:27.14244294" + }, + "versions_index_only": { + "content": [ + [ + "versions.yml:md5,7df9d50209f351e1f75e05a1fad6ba4b" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T15:00:35.609161481" + }, + "versions_single_end_premade_index_stub": { + "content": [ + [ + "versions.yml:md5,7df9d50209f351e1f75e05a1fad6ba4b" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T15:05:02.059858431" + }, + "index_single_end_stub": { + "content": [ + [ + [ + { + "id": "test2" + }, + [ + + ] + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T14:23:58.068772508" + }, + "versions_index_only_stub": { + "content": [ + [ + "versions.yml:md5,7df9d50209f351e1f75e05a1fad6ba4b" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T15:00:47.169402699" + }, + "index_single_end_premade_index_stub": { + "content": [ + [ + [ + { + "id": "test2" + }, + [ + + ] + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T15:05:01.953316205" + }, + "index_single_end": { + "content": [ + [ + [ + { + "id": "test2" + }, + [ + "2415186086593376314.bursttrie_0.dat:md5,74f7f020e8d46e24a8a2e9c5fbcd564a", + "2415186086593376314.kmer_0.dat:md5,4a0bcb71b120f6a6949b7969292ef2e7", + "2415186086593376314.pos_0.dat:md5,bc2875e4cc4017707306565e396839ef", + "2415186086593376314.stats:md5,67c9d4c768f28a450fc82a2b5d43db5c" + ] + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T14:36:26.88061978" + }, + "index_index_only": { + "content": [ + [ + [ + { + "id": "test2" + }, + [ + "2415186086593376314.bursttrie_0.dat:md5,74f7f020e8d46e24a8a2e9c5fbcd564a", + "2415186086593376314.kmer_0.dat:md5,4a0bcb71b120f6a6949b7969292ef2e7", + "2415186086593376314.pos_0.dat:md5,bc2875e4cc4017707306565e396839ef", + "2415186086593376314.stats:md5,67c9d4c768f28a450fc82a2b5d43db5c" + ] + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T15:00:35.34089252" + }, + "sarscov2 single_end_premade_index_match": { + "content": [ + [ + "test.non_rRNA.fastq.gz", "test.sortmerna.log", - "{id=test, single_end=false}" + "{id=test}" ] ], - "timestamp": "2023-12-21T12:00:47.879193" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-26T15:01:53.797737296" } } \ No newline at end of file diff --git a/modules/nf-core/sortmerna/tests/premade_index.config b/modules/nf-core/sortmerna/tests/premade_index.config new file mode 100644 index 0000000..ab86d2e --- /dev/null +++ b/modules/nf-core/sortmerna/tests/premade_index.config @@ -0,0 +1,8 @@ +process { + withName: 'SORTMERNA_INDEX' { + ext.args = '--index 1' + } + withName: 'SORTMERNA' { + ext.args = '--index 0' + } +} diff --git a/modules/nf-core/star/align/environment.yml b/modules/nf-core/star/align/environment.yml index 36fcd02..8bd58cf 100644 --- a/modules/nf-core/star/align/environment.yml +++ b/modules/nf-core/star/align/environment.yml @@ -6,4 +6,5 @@ channels: dependencies: - bioconda::star=2.7.10a - bioconda::samtools=1.18 + - bioconda::htslib=1.18 - conda-forge::gawk=5.1.0 diff --git a/modules/nf-core/star/align/tests/main.nf.test b/modules/nf-core/star/align/tests/main.nf.test index 4c87847..6ecd778 100644 --- a/modules/nf-core/star/align/tests/main.nf.test +++ b/modules/nf-core/star/align/tests/main.nf.test @@ -7,39 +7,40 @@ nextflow_process { tag "modules_nfcore" tag "star" tag "star/align" + tag "star/genomegenerate" - test("homo_sapiens - single_end") { - config "./nextflow.config" - - setup { - run("STAR_GENOMEGENERATE") { - script "../../../star/genomegenerate/main.nf" - process { - """ - input[0] = Channel.of([ - [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] - ]) - input[1] = Channel.of([ - [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] - ]) - """ - } + setup { + run("STAR_GENOMEGENERATE") { + script "../../../star/genomegenerate/main.nf" + process { + """ + input[0] = Channel.of([ + [ id:'test_fasta' ], + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] + ]) + input[1] = Channel.of([ + [ id:'test_gtf' ], + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ] + ]) + """ } } + } + + test("homo_sapiens - single_end") { + config "./nextflow.config" when { process { """ input[0] = Channel.of([ [ id:'test', single_end:true ], // meta map - [ file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_1_fastq_gz'], checkIfExists: true) ] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true) ] ]) input[1] = STAR_GENOMEGENERATE.out.index input[2] = Channel.of([ [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ] ]) input[3] = false input[4] = 'illumina' @@ -74,38 +75,20 @@ nextflow_process { test("homo_sapiens - paired_end") { config "./nextflow.config" - setup { - run("STAR_GENOMEGENERATE") { - script "../../../star/genomegenerate/main.nf" - process { - """ - input[0] = Channel.of([ - [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] - ]) - input[1] = Channel.of([ - [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] - ]) - """ - } - } - } - when { process { """ input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_1_fastq_gz'], checkIfExists: true), - file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true) ] ]) input[1] = STAR_GENOMEGENERATE.out.index input[2] = Channel.of([ [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ] ]) input[3] = false input[4] = 'illumina' @@ -140,38 +123,20 @@ nextflow_process { test("homo_sapiens - paired_end - arriba") { config "./nextflow.arriba.config" - setup { - run("STAR_GENOMEGENERATE") { - script "../../../star/genomegenerate/main.nf" - process { - """ - input[0] = Channel.of([ - [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] - ]) - input[1] = Channel.of([ - [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] - ]) - """ - } - } - } - when { process { """ input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_1_fastq_gz'], checkIfExists: true), - file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true) ] ]) input[1] = STAR_GENOMEGENERATE.out.index input[2] = Channel.of([ [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ] ]) input[3] = false input[4] = 'illumina' @@ -206,38 +171,20 @@ nextflow_process { test("homo_sapiens - paired_end - starfusion") { config "./nextflow.starfusion.config" - setup { - run("STAR_GENOMEGENERATE") { - script "../../../star/genomegenerate/main.nf" - process { - """ - input[0] = Channel.of([ - [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] - ]) - input[1] = Channel.of([ - [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] - ]) - """ - } - } - } - when { process { """ input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_1_fastq_gz'], checkIfExists: true), - file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true) ] ]) input[1] = STAR_GENOMEGENERATE.out.index input[2] = Channel.of([ [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ] ]) input[3] = false input[4] = 'illumina' @@ -272,40 +219,22 @@ nextflow_process { test("homo_sapiens - paired_end - multiple") { config "./nextflow.config" - setup { - run("STAR_GENOMEGENERATE") { - script "../../../star/genomegenerate/main.nf" - process { - """ - input[0] = Channel.of([ - [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] - ]) - input[1] = Channel.of([ - [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] - ]) - """ - } - } - } - when { process { """ input[0] = Channel.of([ [ id:'test', single_end:false ], // meta map [ - file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_1_fastq_gz'], checkIfExists: true), - file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_2_fastq_gz'], checkIfExists: true), - file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_1_fastq_gz'], checkIfExists: true), - file(params.test_data['homo_sapiens']['illumina']['test_rnaseq_2_fastq_gz'], checkIfExists: true) + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/illumina/fastq/test_rnaseq_2.fastq.gz', checkIfExists: true) ] ]) input[1] = STAR_GENOMEGENERATE.out.index input[2] = Channel.of([ [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ] ]) input[3] = false input[4] = 'illumina' diff --git a/modules/nf-core/star/genomegenerate/environment.yml b/modules/nf-core/star/genomegenerate/environment.yml index 93e4476..791f255 100644 --- a/modules/nf-core/star/genomegenerate/environment.yml +++ b/modules/nf-core/star/genomegenerate/environment.yml @@ -1,11 +1,10 @@ name: star_genomegenerate - channels: - conda-forge - bioconda - defaults - dependencies: - bioconda::samtools=1.18 + - bioconda::htslib=1.18 - bioconda::star=2.7.10a - conda-forge::gawk=5.1.0 diff --git a/modules/nf-core/star/genomegenerate/tests/main.nf.test b/modules/nf-core/star/genomegenerate/tests/main.nf.test index af0c942..c17c8ba 100644 --- a/modules/nf-core/star/genomegenerate/tests/main.nf.test +++ b/modules/nf-core/star/genomegenerate/tests/main.nf.test @@ -8,18 +8,18 @@ nextflow_process { tag "star" tag "star/genomegenerate" - test("homo_sapiens") { + test("fasta_gtf") { when { process { """ input[0] = Channel.of([ [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] ]) input[1] = Channel.of([ [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ] ]) """ } @@ -28,14 +28,13 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("index_with_gtf") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_gtf_index") }, + { assert snapshot(process.out.versions).match("fasta_gtf_versions") } ) } - } - test("homo_sapiens-stub") { + test("fasta_gtf_stub") { options '-stub' @@ -44,11 +43,11 @@ nextflow_process { """ input[0] = Channel.of([ [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] ]) input[1] = Channel.of([ [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ] ]) """ } @@ -57,21 +56,20 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("index_with_gtf") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_gtf_stub_index") }, + { assert snapshot(process.out.versions).match("fasta_gtf_stub_versions") } ) } - } - test("homo_sapiens-without_gtf") { + test("fasta") { when { process { """ input[0] = Channel.of([ [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] ]) input[1] = Channel.of([ [], [] ]) """ @@ -81,14 +79,14 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("index_without_gtf") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_index") }, + { assert snapshot(process.out.versions).match("fasta_versions") } ) } } - test("homo_sapiens-without_gtf-stub") { + test("fasta_stub") { options '-stub' @@ -97,7 +95,7 @@ nextflow_process { """ input[0] = Channel.of([ [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] ]) input[1] = Channel.of([ [], [] ]) """ @@ -107,11 +105,11 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("index_without_gtf") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_stub_index") }, + { assert snapshot(process.out.versions).match("fasta_stub_versions") } ) } } -} \ No newline at end of file +} diff --git a/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap b/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap index 9de08c7..5653d6e 100644 --- a/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap +++ b/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap @@ -1,22 +1,90 @@ { - "versions": { + "fasta_gtf_versions": { "content": [ [ "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f" ] ], - "timestamp": "2023-12-19T11:05:51.741109" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:54:31.798555" }, - "index_with_gtf": { + "fasta_stub_versions": { + "content": [ + [ + "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:55:07.521209" + }, + "fasta_gtf_stub_index": { + "content": [ + "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, exonGeTrInfo.tab, exonInfo.tab, geneInfo.tab, genomeParameters.txt, sjdbInfo.txt, sjdbList.fromGTF.out.tab, sjdbList.out.tab, transcriptInfo.tab]" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:54:46.478098" + }, + "fasta_gtf_stub_versions": { + "content": [ + [ + "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:54:46.491657" + }, + "fasta_index": { + "content": [ + "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, genomeParameters.txt]" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:54:57.552329" + }, + "fasta_versions": { + "content": [ + [ + "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:54:57.560541" + }, + "fasta_gtf_index": { "content": [ "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, exonGeTrInfo.tab, exonInfo.tab, geneInfo.tab, genomeParameters.txt, sjdbInfo.txt, sjdbList.fromGTF.out.tab, sjdbList.out.tab, transcriptInfo.tab]" ], - "timestamp": "2023-12-19T11:38:14.551548" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:54:31.786814" }, - "index_without_gtf": { + "fasta_stub_index": { "content": [ "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, genomeParameters.txt]" ], - "timestamp": "2023-12-19T11:38:22.382905" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:55:07.517472" } } \ No newline at end of file diff --git a/modules/nf-core/umitools/extract/environment.yml b/modules/nf-core/umitools/extract/environment.yml index 7d08ac0..aab452d 100644 --- a/modules/nf-core/umitools/extract/environment.yml +++ b/modules/nf-core/umitools/extract/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::umi_tools=1.1.4 + - bioconda::umi_tools=1.1.5 diff --git a/modules/nf-core/umitools/extract/main.nf b/modules/nf-core/umitools/extract/main.nf index 4bd79e7..8719e5f 100644 --- a/modules/nf-core/umitools/extract/main.nf +++ b/modules/nf-core/umitools/extract/main.nf @@ -5,8 +5,8 @@ process UMITOOLS_EXTRACT { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/umi_tools:1.1.4--py38hbff2b2d_1' : - 'biocontainers/umi_tools:1.1.4--py38hbff2b2d_1' }" + 'https://depot.galaxyproject.org/singularity/umi_tools:1.1.5--py39hf95cd2a_0' : + 'biocontainers/umi_tools:1.1.5--py39hf95cd2a_0' }" input: tuple val(meta), path(reads) diff --git a/modules/nf-core/umitools/extract/tests/main.nf.test b/modules/nf-core/umitools/extract/tests/main.nf.test index 22242d1..2a8eba1 100644 --- a/modules/nf-core/umitools/extract/tests/main.nf.test +++ b/modules/nf-core/umitools/extract/tests/main.nf.test @@ -12,24 +12,20 @@ nextflow_process { test("Should run without failures") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = [ [ id:'test', single_end:true ], // meta map - [ file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) ] - ] + [ file(params.modules_testdata_base_path + "genomics/sarscov2/illumina/fastq/test_1.fastq.gz", checkIfExists: true) ] + ] """ } } then { assertAll ( - { assert process.success }, - { assert snapshot(process.out.versions).match("versions") } + { assert process.success }, + { assert snapshot(process.out.versions).match("versions") } ) } - } } \ No newline at end of file diff --git a/modules/nf-core/umitools/extract/tests/main.nf.test.snap b/modules/nf-core/umitools/extract/tests/main.nf.test.snap index 6d5944f..bf82701 100644 --- a/modules/nf-core/umitools/extract/tests/main.nf.test.snap +++ b/modules/nf-core/umitools/extract/tests/main.nf.test.snap @@ -2,9 +2,13 @@ "versions": { "content": [ [ - "versions.yml:md5,5a18da2d3a5a4de15e7aaae9082d7abb" + "versions.yml:md5,568d243174c081a0301e74ed42e59b48" ] ], - "timestamp": "2023-12-08T09:41:43.540658352" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-16T10:01:33.326046137" } } \ No newline at end of file diff --git a/modules/nf-core/umitools/extract/tests/nextflow.config b/modules/nf-core/umitools/extract/tests/nextflow.config index 628f5fc..c866f5a 100644 --- a/modules/nf-core/umitools/extract/tests/nextflow.config +++ b/modules/nf-core/umitools/extract/tests/nextflow.config @@ -1,7 +1,7 @@ process { publishDir = { "${params.outdir}/${task.process.tokenize(':')[-1].tokenize('_')[0].toLowerCase()}" } - + withName: UMITOOLS_EXTRACT { ext.args = '--bc-pattern="NNNN"' } diff --git a/modules/pfr/lai/tests/main.nf.test b/modules/pfr/lai/tests/main.nf.test deleted file mode 100644 index 353043c..0000000 --- a/modules/pfr/lai/tests/main.nf.test +++ /dev/null @@ -1,120 +0,0 @@ -nextflow_process { - - name "Test Process LAI" - script "../main.nf" - process "LAI" - config "./nextflow.config" - - tag "modules" - tag "modules_nfcore" - tag "lai" - tag "gt/suffixerator" - tag "nf-core/gunzip" - tag "gt/ltrharvest" - tag "ltrretriever" - - test("homo_sapiens-genome_21_fasta-success") { - - setup { - run("GUNZIP") { - script "../../../nf-core/gunzip" - - process { - """ - input[0] = [ - [ id:'test' ], - file('/Users/hrauxr/Projects/nxf-modules/tests/data/chr1.fa.gz', checkIfExists: true) - ] - """ - } - } - - run("GT_SUFFIXERATOR") { - script "../../../pfr/gt/suffixerator" - - process { - """ - input[0] = GUNZIP.out.gunzip - """ - } - } - - run("GT_LTRHARVEST") { - script "../../../pfr/gt/ltrharvest" - - process { - """ - input[0] = GT_SUFFIXERATOR.out.index - """ - } - } - - run("LTRRETRIEVER") { - script "../../../pfr/ltrretriever" - - process { - """ - input[0] = GUNZIP.out.gunzip - input[1] = GT_LTRHARVEST.out.tabout.map { meta, tabout -> tabout } - input[2] = [] - input[3] = [] - input[4] = [] - """ - } - } - } - - when { - process { - """ - input[0] = GUNZIP.out.gunzip - input[1] = LTRRETRIEVER.out.pass_list.map { meta, pass_list -> pass_list } - input[2] = LTRRETRIEVER.out.annotation_out.map { meta, annotation_out -> annotation_out } - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert path(process.out.log.get(0).get(1)).getText().contains("Dependency checking: Passed!") }, - { assert path(process.out.log.get(0).get(1)).getText().contains("Calculate LAI:") }, - { assert path(process.out.log.get(0).get(1)).getText().contains("Total LTR sequence content (0%) is too low for accurate LAI calculation") }, - { assert path(process.out.log.get(0).get(1)).getText().contains("Sorry, LAI is not applicable on the current genome assembly.") }, - { assert process.out.lai_out == [] }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - - test("stub") { - - options '-stub' - - when { - process { - """ - input[0] = [ - [ id:'test' ], - file(params.test_data['homo_sapiens']['genome']['genome_21_fasta'], checkIfExists: true) - ] - input[1] = [] - input[2] = [] - input[3] = [] - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert snapshot(process.out.versions).match("versions") } - ) - } - - } - -} diff --git a/modules/pfr/lai/tests/main.nf.test.snap b/modules/pfr/lai/tests/main.nf.test.snap deleted file mode 100644 index 751ddb6..0000000 --- a/modules/pfr/lai/tests/main.nf.test.snap +++ /dev/null @@ -1,10 +0,0 @@ -{ - "versions": { - "content": [ - [ - "versions.yml:md5,2ac93e1e6324236af6f9a794bbac2099" - ] - ], - "timestamp": "2023-12-05T12:15:32.969684" - } -} \ No newline at end of file diff --git a/modules/pfr/lai/tests/nextflow.config b/modules/pfr/lai/tests/nextflow.config deleted file mode 100644 index 516a3e2..0000000 --- a/modules/pfr/lai/tests/nextflow.config +++ /dev/null @@ -1,10 +0,0 @@ -process { - - withName: GT_SUFFIXERATOR { - ext.args = '-tis -suf -lcp -des -ssp -sds -dna' - } - - withName: GT_LTRHARVEST { - ext.args = '-minlenltr 100 -maxlenltr 7000 -mintsd 4 -maxtsd 6 -motif TGCA -motifmis 1 -similar 85 -vic 10 -seed 20 -seqids yes' - } -} diff --git a/modules/pfr/lai/tests/tags.yml b/modules/pfr/lai/tests/tags.yml deleted file mode 100644 index 252295d..0000000 --- a/modules/pfr/lai/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -lai: - - "modules/pfr/lai/**" diff --git a/modules/pfr/lai/environment.yml b/modules/pfr/ltrretriever/lai/environment.yml similarity index 75% rename from modules/pfr/lai/environment.yml rename to modules/pfr/ltrretriever/lai/environment.yml index 94fadbd..e0e4968 100644 --- a/modules/pfr/lai/environment.yml +++ b/modules/pfr/ltrretriever/lai/environment.yml @@ -1,9 +1,9 @@ --- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json -name: "lai" +name: "ltrretriever_lai" channels: - conda-forge - bioconda - defaults dependencies: - - "bioconda::LTR_retriever=2.9.0" + - "bioconda::LTR_retriever=2.9.9" diff --git a/modules/pfr/lai/main.nf b/modules/pfr/ltrretriever/lai/main.nf similarity index 54% rename from modules/pfr/lai/main.nf rename to modules/pfr/ltrretriever/lai/main.nf index d4fced9..464b215 100644 --- a/modules/pfr/lai/main.nf +++ b/modules/pfr/ltrretriever/lai/main.nf @@ -1,11 +1,11 @@ -process LAI { +process LTRRETRIEVER_LAI { tag "$meta.id" - label 'process_high' + label 'process_medium' conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/ltr_retriever:2.9.0--hdfd78af_2': - 'biocontainers/ltr_retriever:2.9.0--hdfd78af_2' }" + 'https://depot.galaxyproject.org/singularity/ltr_retriever:2.9.9--hdfd78af_0': + 'biocontainers/ltr_retriever:2.9.9--hdfd78af_0' }" input: tuple val(meta), path(fasta) @@ -26,44 +26,46 @@ process LAI { def prefix = task.ext.prefix ?: "${meta.id}" def monoploid_param = monoploid_seqs ? "-mono $monoploid_seqs" : '' def lai_output_name = monoploid_seqs ? "${annotation_out}.${monoploid_seqs}.out.LAI" : "${annotation_out}.LAI" + def VERSION = 'beta3.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. """ - # Remove comments from genome fasta, - # otherwise LAI triggers its sequence name change logic - - sed \\ - '/^>/ s/\\s.*\$//' \\ - $fasta \\ - > for_lai_no_comments.fsa - LAI \\ - -genome for_lai_no_comments.fsa \\ + -genome $fasta \\ -intact $pass_list \\ -all $annotation_out \\ -t $task.cpus \\ $monoploid_param \\ $args \\ - > "${prefix}.LAI.log" + > >(tee "${prefix}.LAI.log") \\ + || echo "LAI failed! See ${prefix}.LAI.log" mv \\ $lai_output_name \\ "${prefix}.LAI.out" \\ - || echo "LAI did not produce the output file" + || echo "LAI failed to estimate assembly index. See ${prefix}.LAI.log" cat <<-END_VERSIONS > versions.yml "${task.process}": - lai: \$(cat /usr/local/share/LTR_retriever/LAI | grep "my \\\$version" | sed 's/my \$version="//; s/";//') + lai: $VERSION END_VERSIONS """ stub: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${meta.id}" + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def monoploid_param = monoploid_seqs ? "-mono $monoploid_seqs" : '' + def lai_output_name = monoploid_seqs ? "${annotation_out}.${monoploid_seqs}.out.LAI" : "${annotation_out}.LAI" + def VERSION = 'beta3.2' // WARN: Version information not provided by tool on CLI. Please update this string when bumping container versions. """ - touch ${prefix}.LAI.log + touch "${prefix}.LAI.log" + touch "$lai_output_name" + + mv \\ + $lai_output_name \\ + "${prefix}.LAI.out" cat <<-END_VERSIONS > versions.yml "${task.process}": - lai: \$(cat /usr/local/share/LTR_retriever/LAI | grep "my \\\$version" | sed 's/my \$version="//; s/";//') + lai: $VERSION END_VERSIONS """ } diff --git a/modules/pfr/lai/meta.yml b/modules/pfr/ltrretriever/lai/meta.yml similarity index 91% rename from modules/pfr/lai/meta.yml rename to modules/pfr/ltrretriever/lai/meta.yml index 6fd7aef..f84cf6c 100644 --- a/modules/pfr/lai/meta.yml +++ b/modules/pfr/ltrretriever/lai/meta.yml @@ -1,7 +1,9 @@ --- # yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: "lai" -description: Estimates the mean LTR sequence identity in the genome +name: "ltrretriever_lai" +description: | + Estimates the mean LTR sequence identity in the genome. The input genome fasta should + have short alphanumeric IDs without comments keywords: - genomics - annotation diff --git a/modules/pfr/ltrretriever/lai/tests/main.nf.test b/modules/pfr/ltrretriever/lai/tests/main.nf.test new file mode 100644 index 0000000..df7db2c --- /dev/null +++ b/modules/pfr/ltrretriever/lai/tests/main.nf.test @@ -0,0 +1,166 @@ +nextflow_process { + + name "Test Process LTRRETRIEVER_LAI" + script "../main.nf" + process "LTRRETRIEVER_LAI" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "gunzip" + tag "ltrretriever" + tag "ltrretriever/ltrretriever" + tag "ltrretriever/lai" + tag "ltrharvest" + tag "ltrfinder" + tag "cat/cat" + + test("actinidia_chinensis-genome_21_fasta_gz-success") { + + setup { + + run("GUNZIP") { + script "../../../gunzip" + + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + """ + } + } + + run("LTRHARVEST") { + script "../../../ltrharvest" + + process { + """ + input[0] = GUNZIP.out.gunzip + """ + } + } + + run("LTRFINDER") { + script "../../../ltrfinder" + + process { + """ + input[0] = GUNZIP.out.gunzip + """ + } + } + + run("CAT_CAT") { + script "../../../cat/cat" + + process { + """ + input[0] = LTRHARVEST.out.scn.mix(LTRFINDER.out.scn).groupTuple() + """ + } + } + + run("LTRRETRIEVER_LTRRETRIEVER") { + script "../../ltrretriever" + + process { + """ + input[0] = GUNZIP.out.gunzip + input[1] = CAT_CAT.out.file_out.map { meta, tabout -> tabout } + input[2] = [] + input[3] = [] + input[4] = [] + """ + } + } + } + + when { + process { + """ + input[0] = GUNZIP.out.gunzip + input[1] = LTRRETRIEVER_LTRRETRIEVER.out.pass_list.map { meta, pass_list -> pass_list } + input[2] = LTRRETRIEVER_LTRRETRIEVER.out.annotation_out.map { meta, annotation_out -> annotation_out } + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.log[0][1]).text.contains("Dependency checking: Passed!") }, + { assert path(process.out.log[0][1]).text.contains("Calculate LAI:") }, + { assert path(process.out.log[0][1]).text.contains("Done!") }, + { assert path(process.out.log[0][1]).text.contains("Result file:") }, + { assert Math.abs(Float.parseFloat(path(process.out.lai_out[0][1]).text.split("\n")[1].split("\t")[6]) - 31.29) <= 1.0 } + ) + } + + } + + test("stub") { + + options '-stub' + + when { + process { + """ + def pass_list = new File('test.pass.list') + def out_file = new File('test.out') + def monoploid_seqs = new File('some_seqs.list.txt') + + input[0] = [ + [ id:'test' ], + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + input[1] = pass_list.toPath() + input[2] = out_file.toPath() + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("stub_with_monoploid_seqs") { + + options '-stub' + + when { + process { + """ + def pass_list = new File('test.pass.list') + def out_file = new File('test.out') + def monoploid_seqs = new File('some_seqs.list.txt') + + input[0] = [ + [ id:'test' ], + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + input[1] = pass_list.toPath() + input[2] = out_file.toPath() + input[3] = monoploid_seqs.toPath() + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/pfr/ltrretriever/lai/tests/main.nf.test.snap b/modules/pfr/ltrretriever/lai/tests/main.nf.test.snap new file mode 100644 index 0000000..e1c8086 --- /dev/null +++ b/modules/pfr/ltrretriever/lai/tests/main.nf.test.snap @@ -0,0 +1,100 @@ +{ + "stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e04e27f9408e771795cd44d96518b7cd" + ], + "lai_out": [ + [ + { + "id": "test" + }, + "test.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "log": [ + [ + { + "id": "test" + }, + "test.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e04e27f9408e771795cd44d96518b7cd" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-22T20:09:00.558021" + }, + "stub_with_monoploid_seqs": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,e04e27f9408e771795cd44d96518b7cd" + ], + "lai_out": [ + [ + { + "id": "test" + }, + "test.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "log": [ + [ + { + "id": "test" + }, + "test.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,e04e27f9408e771795cd44d96518b7cd" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-22T20:10:08.213842" + } +} \ No newline at end of file diff --git a/modules/pfr/ltrretriever/lai/tests/nextflow.config b/modules/pfr/ltrretriever/lai/tests/nextflow.config new file mode 100644 index 0000000..75edf1a --- /dev/null +++ b/modules/pfr/ltrretriever/lai/tests/nextflow.config @@ -0,0 +1,15 @@ +process { + + withName: LTRHARVEST { + ext.prefix = { "${meta.id}_ltrharvest" } + } + + withName: LTRFINDER { + ext.args = '-harvest_out -size 1000000 -time 300' + // recommended parameters: https://github.com/oushujun/LTR_retriever#usage + } + + withName: CAT_CAT { + ext.prefix = { "${meta.id}_ltrharvest_ltrfinder.tabout" } + } +} diff --git a/modules/pfr/ltrretriever/lai/tests/tags.yml b/modules/pfr/ltrretriever/lai/tests/tags.yml new file mode 100644 index 0000000..470f468 --- /dev/null +++ b/modules/pfr/ltrretriever/lai/tests/tags.yml @@ -0,0 +1,2 @@ +ltrretriever/lai: + - "modules/nf-core/ltrretriever/lai/**" diff --git a/subworkflows/local/preprocess_rnaseq.nf b/subworkflows/local/preprocess_rnaseq.nf index 9466104..81ff0d8 100644 --- a/subworkflows/local/preprocess_rnaseq.nf +++ b/subworkflows/local/preprocess_rnaseq.nf @@ -84,7 +84,8 @@ workflow PREPROCESS_RNASEQ { // MODULE: SORTMERNA SORTMERNA( remove_ribo_rna ? ch_trim_reads : Channel.empty(), - sortmerna_fastas + sortmerna_fastas.map { fastas -> [ [], fastas ] }, + [ [], [] ] ) ch_emitted_reads = remove_ribo_rna diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf index 2c67b3c..833d82b 100644 --- a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf +++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/main.nf @@ -20,6 +20,19 @@ def getFastpReadsAfterFiltering(json_file, min_trimmed_reads) { return json['after_filtering']['total_reads'].toLong() } +def getFastpAdapterSequence(json_file){ + + if (!json_file.text) { return "" } // Usman Rashid: To allow -stub with FASTP + + def Map json = (Map) new JsonSlurper().parseText(json_file.text) + try{ + adapter = json['adapter_cutting']['read1_adapter_sequence'] + } catch(Exception ex){ + adapter = "" + } + return adapter +} + workflow FASTQ_FASTQC_UMITOOLS_FASTP { take: reads // channel: [ val(meta), [ reads ] ] @@ -28,7 +41,7 @@ workflow FASTQ_FASTQC_UMITOOLS_FASTP { skip_umi_extract // boolean: true/false umi_discard_read // integer: 0, 1 or 2 skip_trimming // boolean: true/false - adapter_fasta // file: adapter.fasta + adapter_fasta // file: adapter.fasta save_trimmed_fail // boolean: true/false save_merged // boolean: true/false min_trimmed_reads // integer: > 0 @@ -78,6 +91,8 @@ workflow FASTQ_FASTQC_UMITOOLS_FASTP { fastqc_trim_html = Channel.empty() fastqc_trim_zip = Channel.empty() trim_read_count = Channel.empty() + adapter_seq = Channel.empty() + if (!skip_trimming) { FASTP ( umi_reads, @@ -111,6 +126,10 @@ workflow FASTQ_FASTQC_UMITOOLS_FASTP { .map { meta, reads, num_reads -> [ meta, num_reads ] } .set { trim_read_count } + trim_json + .map { meta, json -> [meta, getFastpAdapterSequence(json)] } + .set { adapter_seq } + if (!skip_fastqc) { FASTQC_TRIM ( trim_reads @@ -128,6 +147,7 @@ workflow FASTQ_FASTQC_UMITOOLS_FASTP { fastqc_raw_zip // channel: [ val(meta), [ zip ] ] umi_log // channel: [ val(meta), [ log ] ] + adapter_seq // channel: [ val(meta), [ adapter_seq] ] trim_json // channel: [ val(meta), [ json ] ] trim_html // channel: [ val(meta), [ html ] ] diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml index 220e8db..9308fe9 100644 --- a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml +++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/meta.yml @@ -43,7 +43,7 @@ input: - skip_trimming: type: boolean description: | - Allows to skip trimgalore execution + Allows to skip FastP execution - adapter_fasta: type: file description: | @@ -70,10 +70,7 @@ output: type: file description: > Extracted FASTQ files. | For single-end reads, pattern is \${prefix}.umi_extract.fastq.gz. | - - - - For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz. + For paired-end reads, pattern is \${prefix}.umi_extract_{1,2}.fastq.gz. pattern: "*.{fastq.gz}" - fastqc_html: type: file @@ -118,6 +115,10 @@ output: type: file description: FastQC report archive pattern: "*_{fastqc.zip}" + - adapter_seq: + type: string + description: | + Adapter Sequence found in read1 - versions: type: file description: File containing software versions diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test index cdd7398..961b5b4 100644 --- a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test +++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test @@ -3,6 +3,8 @@ nextflow_workflow { name "Test Workflow FASTQ_FASTQC_UMITOOLS_FASTP" script "../main.nf" workflow "FASTQ_FASTQC_UMITOOLS_FASTP" + config './nextflow.config' + tag "subworkflows" tag "subworkflows_nfcore" tag "subworkflows/fastq_fastqc_umitools_fastp" @@ -17,22 +19,518 @@ nextflow_workflow { when { workflow { """ - input[0] = [ - [ id:'test', single_end:false ], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) - ] - ] - input[1] = false // skip_fastqc - input[2] = false // with_umi - input[3] = false // skip_umi_extract - input[4] = 1 // umi_discard_read - input[5] = false // skip_trimming - input[6] = [] // adapter_fasta - input[7] = false // save_trimmed_fail - input[8] = false // save_merged - input[9] = 1 // min_trimmed_reads + skip_fastqc = false + with_umi = false + skip_umi_extract = false + umi_discard_read = 1 + skip_trimming = false + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + min_trimmed_reads = 1 + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = skip_fastqc + input[2] = with_umi + input[3] = skip_umi_extract + input[4] = umi_discard_read + input[5] = skip_trimming + input[6] = adapter_fasta + input[7] = save_trimmed_fail + input[8] = save_merged + input[9] = min_trimmed_reads + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot( + workflow.out.reads, + workflow.out.umi_log, + workflow.out.trim_json, + workflow.out.trim_reads_fail, + workflow.out.trim_reads_merged, + workflow.out.adapter_seq, + workflow.out.trim_read_count, + workflow.out.versions + ).match() + }, + + { assert workflow.out.fastqc_raw_html }, + { assert workflow.out.fastqc_raw_zip }, + { assert workflow.out.trim_html }, + { assert workflow.out.trim_log }, + { assert workflow.out.fastqc_trim_html }, + { assert workflow.out.fastqc_trim_zip } + ) + } + } + + test("skip_fastqc") { + + when { + workflow { + """ + skip_fastqc = true + with_umi = false + skip_umi_extract = false + umi_discard_read = 1 + skip_trimming = false + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + min_trimmed_reads = 1 + + input[0] = Channel.of([ + [ id:'test', single_end: false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = skip_fastqc + input[2] = with_umi + input[3] = skip_umi_extract + input[4] = umi_discard_read + input[5] = skip_trimming + input[6] = adapter_fasta + input[7] = save_trimmed_fail + input[8] = save_merged + input[9] = min_trimmed_reads + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot( + workflow.out.reads, + workflow.out.umi_log, + workflow.out.trim_json, + workflow.out.trim_reads_fail, + workflow.out.trim_reads_merged, + workflow.out.adapter_seq, + workflow.out.trim_read_count, + workflow.out.versions + ).match() + }, + + { assert !workflow.out.fastqc_raw_html }, + { assert !workflow.out.fastqc_raw_zip }, + { assert workflow.out.trim_html }, + { assert workflow.out.trim_log }, + { assert !workflow.out.fastqc_trim_html }, + { assert !workflow.out.fastqc_trim_zip } + ) + } + } + + test("with_umi") { + + when { + workflow { + """ + skip_fastqc = false + with_umi = true + skip_umi_extract = false + umi_discard_read = 1 + skip_trimming = false + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + min_trimmed_reads = 1 + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = skip_fastqc + input[2] = with_umi + input[3] = skip_umi_extract + input[4] = umi_discard_read + input[5] = skip_trimming + input[6] = adapter_fasta + input[7] = save_trimmed_fail + input[8] = save_merged + input[9] = min_trimmed_reads + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot( + workflow.out.reads, + workflow.out.trim_json, + workflow.out.trim_reads_fail, + workflow.out.trim_reads_merged, + workflow.out.adapter_seq, + workflow.out.trim_read_count, + workflow.out.versions + ).match() + }, + + { assert workflow.out.fastqc_raw_html }, + { assert workflow.out.fastqc_raw_zip }, + { assert workflow.out.trim_html }, + { assert workflow.out.trim_log }, + { assert workflow.out.fastqc_trim_html }, + { assert workflow.out.fastqc_trim_zip } + ) + } + } + + + test("skip_umi_extract") { + + when { + workflow { + """ + skip_fastqc = false + with_umi = true + skip_umi_extract = true + umi_discard_read = 1 + skip_trimming = false + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + min_trimmed_reads = 1 + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = skip_fastqc + input[2] = with_umi + input[3] = skip_umi_extract + input[4] = umi_discard_read + input[5] = skip_trimming + input[6] = adapter_fasta + input[7] = save_trimmed_fail + input[8] = save_merged + input[9] = min_trimmed_reads + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot( + workflow.out.reads, + workflow.out.umi_log, + workflow.out.trim_json, + workflow.out.trim_reads_fail, + workflow.out.trim_reads_merged, + workflow.out.adapter_seq, + workflow.out.trim_read_count, + workflow.out.versions + ).match() + }, + + { assert workflow.out.fastqc_raw_html }, + { assert workflow.out.fastqc_raw_zip }, + { assert workflow.out.trim_html }, + { assert workflow.out.trim_log }, + { assert workflow.out.fastqc_trim_html }, + { assert workflow.out.fastqc_trim_zip } + ) + } + } + + test("umi_discard_read = 2") { + + when { + workflow { + """ + skip_fastqc = false + with_umi = true + skip_umi_extract = true + umi_discard_read = 2 + skip_trimming = false + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + min_trimmed_reads = 1 + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = skip_fastqc + input[2] = with_umi + input[3] = skip_umi_extract + input[4] = umi_discard_read + input[5] = skip_trimming + input[6] = adapter_fasta + input[7] = save_trimmed_fail + input[8] = save_merged + input[9] = min_trimmed_reads + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot( + workflow.out.reads, + workflow.out.umi_log, + workflow.out.trim_json, + workflow.out.trim_reads_fail, + workflow.out.trim_reads_merged, + workflow.out.adapter_seq, + workflow.out.trim_read_count, + workflow.out.versions + ).match() + }, + + { assert workflow.out.fastqc_raw_html }, + { assert workflow.out.fastqc_raw_zip }, + { assert workflow.out.trim_html }, + { assert workflow.out.trim_log }, + { assert workflow.out.fastqc_trim_html }, + { assert workflow.out.fastqc_trim_zip } + ) + } + } + + test("skip_trimming") { + + when { + workflow { + """ + skip_fastqc = false + with_umi = false + skip_umi_extract = false + umi_discard_read = 1 + skip_trimming = true + adapter_fasta = [] + save_trimmed_fail = false + save_merged = false + min_trimmed_reads = 1 + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = skip_fastqc + input[2] = with_umi + input[3] = skip_umi_extract + input[4] = umi_discard_read + input[5] = skip_trimming + input[6] = adapter_fasta + input[7] = save_trimmed_fail + input[8] = save_merged + input[9] = min_trimmed_reads + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot( + workflow.out.reads.get(0).get(0), // Reads meta map + // Because the input file is passed to the output file, we have to do check the filename only + file(workflow.out.reads.get(0).get(1).get(0)).name, + file(workflow.out.reads.get(0).get(1).get(1)).name, + workflow.out.umi_log, + workflow.out.trim_json, + workflow.out.trim_reads_fail, + workflow.out.trim_reads_merged, + workflow.out.adapter_seq, + workflow.out.trim_read_count, + workflow.out.versions + ).match() + }, + + { assert workflow.out.fastqc_raw_html }, + { assert workflow.out.fastqc_raw_zip }, + { assert !workflow.out.trim_html }, + { assert !workflow.out.trim_log }, + { assert !workflow.out.fastqc_trim_html }, + { assert !workflow.out.fastqc_trim_zip } + ) + } + } + + test("save_trimmed_fail") { + + config './nextflow.save_trimmed.config' + + when { + workflow { + """ + skip_fastqc = false + with_umi = false + skip_umi_extract = false + umi_discard_read = 1 + skip_trimming = false + adapter_fasta = [] + save_trimmed_fail = true + save_merged = false + min_trimmed_reads = 1 + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = skip_fastqc + input[2] = with_umi + input[3] = skip_umi_extract + input[4] = umi_discard_read + input[5] = skip_trimming + input[6] = adapter_fasta + input[7] = save_trimmed_fail + input[8] = save_merged + input[9] = min_trimmed_reads + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot( + workflow.out.reads, + workflow.out.umi_log, + workflow.out.trim_json, + workflow.out.trim_reads_fail, + workflow.out.trim_reads_merged, + workflow.out.adapter_seq, + workflow.out.trim_read_count, + workflow.out.versions + ).match() + }, + + { assert workflow.out.fastqc_raw_html }, + { assert workflow.out.fastqc_raw_zip }, + { assert workflow.out.trim_html }, + { assert workflow.out.trim_log }, + { assert workflow.out.fastqc_trim_html }, + { assert workflow.out.fastqc_trim_zip } + ) + } + } + + test("save_merged") { + + when { + workflow { + """ + skip_fastqc = false + with_umi = false + skip_umi_extract = false + umi_discard_read = 1 + skip_trimming = false + adapter_fasta = [] + save_trimmed_fail = false + save_merged = true + min_trimmed_reads = 1 + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = skip_fastqc + input[2] = with_umi + input[3] = skip_umi_extract + input[4] = umi_discard_read + input[5] = skip_trimming + input[6] = adapter_fasta + input[7] = save_trimmed_fail + input[8] = save_merged + input[9] = min_trimmed_reads + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot( + workflow.out.reads, + workflow.out.umi_log, + workflow.out.trim_json, + workflow.out.trim_reads_fail, + workflow.out.trim_reads_merged, + workflow.out.adapter_seq, + workflow.out.trim_read_count, + workflow.out.versions + ).match() + }, + + { assert workflow.out.fastqc_raw_html }, + { assert workflow.out.fastqc_raw_zip }, + { assert workflow.out.trim_html }, + { assert workflow.out.trim_log }, + { assert workflow.out.fastqc_trim_html }, + { assert workflow.out.fastqc_trim_zip } + ) + } + } + + test("min_trimmed_reads = 26") { + // Subworkflow should stop after FASTP which trims down to 25 reads + + when { + workflow { + """ + skip_fastqc = false + with_umi = false + skip_umi_extract = false + umi_discard_read = 1 + skip_trimming = false + adapter_fasta = [] + save_trimmed_fail = false + save_merged = true + min_trimmed_reads = 26 + + input[0] = Channel.of([ + [ id:'test', single_end:false ], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) + ] + ]) + input[1] = skip_fastqc + input[2] = with_umi + input[3] = skip_umi_extract + input[4] = umi_discard_read + input[5] = skip_trimming + input[6] = adapter_fasta + input[7] = save_trimmed_fail + input[8] = save_merged + input[9] = min_trimmed_reads """ } } @@ -40,13 +538,17 @@ nextflow_workflow { then { assertAll( { assert workflow.success }, - { assert snapshot(workflow.out.reads).match("reads") }, - { assert snapshot(workflow.out.umi_log).match("umi_log") }, - { assert snapshot(workflow.out.trim_json).match("trim_json") }, - { assert snapshot(workflow.out.trim_reads_fail).match("trim_reads_fail") }, - { assert snapshot(workflow.out.trim_reads_merged).match("trim_reads_merged") }, - { assert snapshot(workflow.out.trim_read_count).match("trim_read_count") }, - { assert snapshot(workflow.out.versions).match("versions") }, + { assert snapshot( + workflow.out.reads, + workflow.out.umi_log, + workflow.out.trim_json, + workflow.out.trim_reads_fail, + workflow.out.trim_reads_merged, + workflow.out.adapter_seq, + workflow.out.trim_read_count, + workflow.out.versions + ).match() + }, { assert workflow.out.fastqc_raw_html }, { assert workflow.out.fastqc_raw_zip }, diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap index 38a65ae..3e11d9e 100644 --- a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap +++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/main.nf.test.snap @@ -1,32 +1,215 @@ { - "trim_reads_merged": { + "skip_fastqc": { "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1.fastp.fastq.gz:md5,67b2bbae47f073e05a97a9c2edce23c7", + "test_2.fastp.fastq.gz:md5,25cbdca08e2083dbd4f0502de6b62f39" + ] + ] + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test.fastp.json:md5,1e0f8e27e71728e2b63fc64086be95cd" + ] + ], [ + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "unspecified" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + 198 + ] + ], + [ + "versions.yml:md5,85bd0117e5778fff18e3920972a296ad" ] ], - "timestamp": "2023-11-26T02:28:26.26920982" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T16:53:49.315194" }, - "trim_reads_fail": { + "save_trimmed_fail": { "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1.fastp.fastq.gz:md5,6ff32a64c5188b9a9192be1398c262c7", + "test_2.fastp.fastq.gz:md5,db0cb7c9977e94ac2b4b446ebd017a8a" + ] + ] + ], [ + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test.fastp.json:md5,4c3268ddb50ea5b33125984776aa3519" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test.paired.fail.fastq.gz:md5,409b687c734cedd7a1fec14d316e1366", + "test_1.fail.fastq.gz:md5,4f273cf3159c13f79e8ffae12f5661f6", + "test_2.fail.fastq.gz:md5,f97b9edefb5649aab661fbc9e71fc995" + ] + ] + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "unspecified" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + 162 + ] + ], + [ + "versions.yml:md5,85bd0117e5778fff18e3920972a296ad", + "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0", + "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e" ] ], - "timestamp": "2023-11-26T02:28:26.25861515" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T16:51:45.34934" }, - "versions": { + "skip_umi_extract": { "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1.fastp.fastq.gz:md5,67b2bbae47f073e05a97a9c2edce23c7", + "test_2.fastp.fastq.gz:md5,25cbdca08e2083dbd4f0502de6b62f39" + ] + ] + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test.fastp.json:md5,1e0f8e27e71728e2b63fc64086be95cd" + ] + ], + [ + + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "unspecified" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + 198 + ] + ], [ "versions.yml:md5,85bd0117e5778fff18e3920972a296ad", "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0", "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e" ] ], - "timestamp": "2023-11-26T02:28:26.30891403" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T12:07:40.34249" }, - "trim_json": { + "umi_discard_read = 2": { "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1.fastp.fastq.gz:md5,67b2bbae47f073e05a97a9c2edce23c7", + "test_2.fastp.fastq.gz:md5,25cbdca08e2083dbd4f0502de6b62f39" + ] + ] + ], + [ + + ], [ [ { @@ -35,11 +218,44 @@ }, "test.fastp.json:md5,1e0f8e27e71728e2b63fc64086be95cd" ] + ], + [ + + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "unspecified" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + 198 + ] + ], + [ + "versions.yml:md5,85bd0117e5778fff18e3920972a296ad", + "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0", + "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e" ] ], - "timestamp": "2023-11-26T02:28:26.24768259" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T12:08:24.141938" }, - "reads": { + "save_merged": { "content": [ [ [ @@ -48,24 +264,142 @@ "single_end": false }, [ - "test_1.fastp.fastq.gz:md5,67b2bbae47f073e05a97a9c2edce23c7", - "test_2.fastp.fastq.gz:md5,25cbdca08e2083dbd4f0502de6b62f39" + "test_1.fastp.fastq.gz:md5,54b726a55e992a869fd3fa778afe1672", + "test_2.fastp.fastq.gz:md5,29d3b33b869f7b63417b8ff07bb128ba" ] ] + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test.fastp.json:md5,b712fd68ed0322f4bec49ff2a5237fcc" + ] + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test.merged.fastq.gz:md5,c873bb1ab3fa859dcc47306465e749d5" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "unspecified" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + 75 + ] + ], + [ + "versions.yml:md5,85bd0117e5778fff18e3920972a296ad", + "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0", + "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e" ] ], - "timestamp": "2023-12-04T11:30:32.061644815" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T12:10:18.546963" }, - "umi_log": { + "skip_trimming": { "content": [ + { + "id": "test", + "single_end": false + }, + "test_1.fastq.gz", + "test_2.fastq.gz", + [ + + ], + [ + + ], + [ + + ], + [ + + ], + [ + + ], [ + ], + [ + "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e" ] ], - "timestamp": "2023-11-26T02:28:26.238536" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-19T15:49:26.574759" }, - "trim_read_count": { + "sarscov2 paired-end [fastq]": { "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1.fastp.fastq.gz:md5,67b2bbae47f073e05a97a9c2edce23c7", + "test_2.fastp.fastq.gz:md5,25cbdca08e2083dbd4f0502de6b62f39" + ] + ] + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test.fastp.json:md5,1e0f8e27e71728e2b63fc64086be95cd" + ] + ], + [ + + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "unspecified" + ] + ], [ [ { @@ -74,8 +408,142 @@ }, 198 ] + ], + [ + "versions.yml:md5,85bd0117e5778fff18e3920972a296ad", + "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0", + "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T16:53:39.139038" + }, + "min_trimmed_reads = 26": { + "content": [ + [ + [ + { + "id": "test", + "single_end": false + }, + [ + "test_1.fastp.fastq.gz:md5,54b726a55e992a869fd3fa778afe1672", + "test_2.fastp.fastq.gz:md5,29d3b33b869f7b63417b8ff07bb128ba" + ] + ] + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test.fastp.json:md5,b712fd68ed0322f4bec49ff2a5237fcc" + ] + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "test.merged.fastq.gz:md5,c873bb1ab3fa859dcc47306465e749d5" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + "unspecified" + ] + ], + [ + [ + { + "id": "test", + "single_end": false + }, + 75 + ] + ], + [ + "versions.yml:md5,85bd0117e5778fff18e3920972a296ad", + "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0", + "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T11:52:23.849945" + }, + "with_umi": { + "content": [ + [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastp.fastq.gz:md5,ba8c6c3a7ce718d9a2c5857e2edf53bc" + ] + ], + [ + [ + { + "id": "test", + "single_end": true + }, + "test.fastp.json:md5,d39c5c6d9a2e35fb60d26ced46569af6" + ] + ], + [ + + ], + [ + + ], + [ + [ + { + "id": "test", + "single_end": true + }, + "" + ] + ], + [ + [ + { + "id": "test", + "single_end": true + }, + 99 + ] + ], + [ + "versions.yml:md5,01f264f78de3c6d893c449cc6d3cd721", + "versions.yml:md5,85bd0117e5778fff18e3920972a296ad", + "versions.yml:md5,c50aa59475ab901bc6f9a2cf7b1a14e0", + "versions.yml:md5,f3dcaae948e8eed92b4a5557b4c6668e" ] ], - "timestamp": "2023-11-26T02:28:26.27984169" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T17:31:09.193212" } } \ No newline at end of file diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/nextflow.config b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/nextflow.config new file mode 100644 index 0000000..12f7b25 --- /dev/null +++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/nextflow.config @@ -0,0 +1,11 @@ +process { + + withName: UMITOOLS_EXTRACT { + ext.args = '--bc-pattern="NNNN" --bc-pattern2="NNNN"' + } + + withName: UMICOLLAPSE { + ext.prefix = { "${meta.id}.dedup" } + } + +} \ No newline at end of file diff --git a/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/nextflow.save_trimmed.config b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/nextflow.save_trimmed.config new file mode 100644 index 0000000..2430e9d --- /dev/null +++ b/subworkflows/nf-core/fastq_fastqc_umitools_fastp/tests/nextflow.save_trimmed.config @@ -0,0 +1,6 @@ +process { + // Make filtering more aggressive to make more reads fail + withName: FASTP { + ext.args = "-e 30" + } +} \ No newline at end of file diff --git a/subworkflows/pfr/fasta_edta_lai/main.nf b/subworkflows/pfr/fasta_edta_lai/main.nf index 2e73ca5..628e255 100644 --- a/subworkflows/pfr/fasta_edta_lai/main.nf +++ b/subworkflows/pfr/fasta_edta_lai/main.nf @@ -1,33 +1,45 @@ -include { CUSTOM_SHORTENFASTAIDS } from '../../../modules/pfr/custom/shortenfastaids' -include { EDTA_EDTA } from '../../../modules/pfr/edta/edta' -include { LAI } from '../../../modules/pfr/lai' -include { CUSTOM_RESTOREGFFIDS } from '../../../modules/pfr/custom/restoregffids' +include { CUSTOM_SHORTENFASTAIDS } from '../../../modules/pfr/custom/shortenfastaids/main' +include { EDTA_EDTA } from '../../../modules/pfr/edta/edta/main' +include { LTRRETRIEVER_LAI } from '../../../modules/pfr/ltrretriever/lai/main' +include { CUSTOM_RESTOREGFFIDS } from '../../../modules/pfr/custom/restoregffids/main' workflow FASTA_EDTA_LAI { take: - ch_fasta // channel: [ val(meta), fasta ] - ch_monoploid_seqs // channel: [ val(meta), txt ]; Optional: Set to [] if not needed - skip_lai // val; true|false + ch_fasta // channel: [ val(meta), fasta ] + ch_monoploid_seqs // channel: [ val(meta), txt ]; Optional: Set to [] if not needed + skip_lai // val; true|false main: - - ch_versions = Channel.empty() + ch_versions = Channel.empty() // MOUDLE: CUSTOM_SHORTENFASTAIDS CUSTOM_SHORTENFASTAIDS ( ch_fasta ) - ch_short_ids_fasta = ch_fasta - | join(CUSTOM_SHORTENFASTAIDS.out.short_ids_fasta, by:0, remainder:true) - | map { meta, fasta, short_ids_fasta -> - [ meta, short_ids_fasta ?: fasta ] - } + ch_short_ids_fasta = ch_fasta + | join(CUSTOM_SHORTENFASTAIDS.out.short_ids_fasta, by:0, remainder:true) + | map { meta, fasta, short_ids_fasta -> + if ( fasta ) { [ meta, short_ids_fasta ?: fasta ] } + } + + ch_short_ids_tsv = CUSTOM_SHORTENFASTAIDS.out.short_ids_tsv + ch_short_monoploid_seqs = ch_short_ids_tsv + | join( + ch_monoploid_seqs ?: Channel.empty() + ) + | map { meta, short_ids_tsv, monoploid_seqs -> + map_monoploid_seqs_to_new_ids(meta, short_ids_tsv, monoploid_seqs) + } + | collectFile(newLine:true) + | map { seqs -> + def id = seqs.name.split('.mapped.monoploid.seqs.txt')[0] - ch_short_ids_tsv = CUSTOM_SHORTENFASTAIDS.out.short_ids_tsv - ch_versions = ch_versions.mix(CUSTOM_SHORTENFASTAIDS.out.versions.first()) + [ [ id: id ], seqs ] + } + ch_versions = ch_versions.mix(CUSTOM_SHORTENFASTAIDS.out.versions.first()) // MODULE: EDTA_EDTA - EDTA_EDTA ( + EDTA_EDTA( ch_short_ids_fasta, [], [], @@ -35,54 +47,85 @@ workflow FASTA_EDTA_LAI { [] ) - ch_te_lib_fasta = EDTA_EDTA.out.te_lib_fasta - ch_pass_list = EDTA_EDTA.out.pass_list - ch_out_file = EDTA_EDTA.out.out_file - ch_te_anno_gff3 = EDTA_EDTA.out.te_anno_gff3 - ch_versions = ch_versions.mix(EDTA_EDTA.out.versions.first()) + ch_te_lib_fasta = EDTA_EDTA.out.te_lib_fasta + ch_pass_list = EDTA_EDTA.out.pass_list + ch_out_file = EDTA_EDTA.out.out_file + ch_te_anno_gff3 = EDTA_EDTA.out.te_anno_gff3 + ch_versions = ch_versions.mix(EDTA_EDTA.out.versions.first()) // MODULE: LAI - ch_lai_inputs = skip_lai - ? Channel.empty() - : ch_short_ids_fasta - | join(ch_pass_list) - | join(ch_out_file) - | join( - ch_monoploid_seqs ?: Channel.empty(), - by:0, - remainder: true - ) - | map { meta, fasta, pass, out, mono -> - [ meta, fasta, pass, out, mono ?: [] ] - } - LAI ( + ch_lai_inputs = skip_lai + ? Channel.empty() + : ch_short_ids_fasta + | join(ch_pass_list) + | join(ch_out_file) + | map { meta, fasta, pass, out -> + [ meta.id, meta, fasta, pass, out ] + } + | join( + ch_short_monoploid_seqs + | map { meta, mono -> [ meta.id, mono ] }, + by:0, + remainder: true + ) + | map { id, meta, fasta, pass, out, mono -> + [ meta, fasta, pass, out, mono ?: [] ] + } + LTRRETRIEVER_LAI( ch_lai_inputs.map { meta, fasta, pass, out, mono -> [ meta, fasta ] }, ch_lai_inputs.map { meta, fasta, pass, out, mono -> pass }, ch_lai_inputs.map { meta, fasta, pass, out, mono -> out }, ch_lai_inputs.map { meta, fasta, pass, out, mono -> mono } ) - ch_lai_log = LAI.out.log - ch_lai_out = LAI.out.lai_out - ch_versions = ch_versions.mix(LAI.out.versions.first()) + ch_lai_log = LTRRETRIEVER_LAI.out.log + ch_lai_out = LTRRETRIEVER_LAI.out.lai_out + ch_versions = ch_versions.mix(LTRRETRIEVER_LAI.out.versions.first()) // MODULE: CUSTOM_RESTOREGFFIDS - ch_restorable_gff_tsv = ch_te_anno_gff3.join(ch_short_ids_tsv) + ch_restorable_gff_tsv = ch_te_anno_gff3.join(ch_short_ids_tsv) CUSTOM_RESTOREGFFIDS ( ch_restorable_gff_tsv.map { meta, gff, tsv -> [ meta, gff ] }, ch_restorable_gff_tsv.map { meta, gff, tsv -> tsv } ) - ch_restored_gff = ch_te_anno_gff3 - | join(CUSTOM_RESTOREGFFIDS.out.restored_ids_gff3, by:0, remainder:true) - | map { meta, gff, restored_gff -> [ meta, restored_gff ?: gff ] } - ch_versions = ch_versions.mix(CUSTOM_RESTOREGFFIDS.out.versions.first()) + ch_restored_gff = ch_te_anno_gff3 + | join(CUSTOM_RESTOREGFFIDS.out.restored_ids_gff3, by:0, remainder:true) + | map { meta, gff, restored_gff -> [ meta, restored_gff ?: gff ] } + ch_versions = ch_versions.mix(CUSTOM_RESTOREGFFIDS.out.versions.first()) emit: - te_lib_fasta = ch_te_lib_fasta // channel: [ val(meta), fasta ] - te_anno_gff3 = ch_restored_gff // channel: [ val(meta), gff ] - lai_log = ch_lai_log // channel: [ val(meta), log ] - lai_out = ch_lai_out // channel: [ val(meta), out ] - versions = ch_versions // channel: [ versions.yml ] + te_lib_fasta = ch_te_lib_fasta // channel: [ val(meta), fasta ] + te_anno_gff3 = ch_restored_gff // channel: [ val(meta), gff ] + lai_log = ch_lai_log // channel: [ val(meta), log ] + lai_out = ch_lai_out // channel: [ val(meta), out ] + versions = ch_versions // channel: [ versions.yml ] +} + +def map_monoploid_seqs_to_new_ids(meta, short_ids_tsv, monoploid_seqs) { + + def short_ids_head = short_ids_tsv.text.split('\n')[0] + + if (short_ids_head == "IDs have acceptable length and character. No change required.") { + return [ "${meta.id}.mapped.monoploid.seqs.txt" ] + monoploid_seqs.text.split('\n') + } + + def orig_to_new_ids = [:] + short_ids_tsv.text.eachLine { line -> + def (original_id, renamed_id) = line.split('\t') + orig_to_new_ids[original_id] = renamed_id + } + + def mapped_ids = [] + monoploid_seqs.text.eachLine { original_id -> + if (!orig_to_new_ids[original_id]) { + error "Faild to find $original_id in ${monoploid_seqs}" + + "The monoploid_seqs file is malformed!" + } + + mapped_ids.add(orig_to_new_ids[original_id]) + } + + return [ "${meta.id}.mapped.monoploid.seqs.txt" ] + mapped_ids } diff --git a/subworkflows/pfr/fasta_edta_lai/meta.yml b/subworkflows/pfr/fasta_edta_lai/meta.yml index 52483ce..c356ce7 100644 --- a/subworkflows/pfr/fasta_edta_lai/meta.yml +++ b/subworkflows/pfr/fasta_edta_lai/meta.yml @@ -11,10 +11,10 @@ keywords: - stats - qc components: + - edta/edta - custom/restoregffids + - ltrretriever/lai - custom/shortenfastaids - - edta/edta - - lai input: - ch_fasta: type: file diff --git a/subworkflows/pfr/fasta_edta_lai/tests/main.nf.test b/subworkflows/pfr/fasta_edta_lai/tests/main.nf.test index e852a70..d968790 100644 --- a/subworkflows/pfr/fasta_edta_lai/tests/main.nf.test +++ b/subworkflows/pfr/fasta_edta_lai/tests/main.nf.test @@ -3,17 +3,60 @@ nextflow_workflow { name "Test Workflow FASTA_EDTA_LAI" script "../main.nf" workflow "FASTA_EDTA_LAI" + config "./nextflow.config" tag "subworkflows" tag "subworkflows_nfcore" tag "subworkflows/fasta_edta_lai" tag "fasta_edta_lai" - tag "lai" + tag "modules/nf-core/gunzip" + tag "custom/shortenfastaids" tag "edta/edta" + tag "ltrretriever/lai" tag "custom/restoregffids" - tag "custom/shortenfastaids" - test("test_data") { + test("actinidia_chinensis-genome_21_fasta_gz") { + + setup { + run("GUNZIP") { + script "../../../../modules/nf-core/gunzip" + + process { + """ + input[0] = [ + [ id:'test' ], + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) + ] + """ + } + } + } + + when { + workflow { + """ + input[0] = GUNZIP.out.gunzip + input[1] = [] + input[2] = false + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert file(workflow.out.te_anno_gff3[0][1]).text.contains('Copia_LTR_retrotransposon') }, + { assert file(workflow.out.lai_log[0][1]).text.contains('Calculate LAI:') }, + { assert file(workflow.out.lai_log[0][1]).text.contains('Done!') }, + { assert Math.abs(Float.parseFloat(path(workflow.out.lai_out[0][1]).text.split("\n")[1].split("\t")[6]) - 31.29) <= 1.0 }, + { assert file(workflow.out.te_lib_fasta[0][1]).text.contains('#LTR/Copia') } + ) + } + } + + test("actinidia_chinensis-genome_21_fasta_gz-stub") { + + options '-stub' setup { run("GUNZIP") { @@ -23,7 +66,7 @@ nextflow_workflow { """ input[0] = [ [ id:'test' ], - file('/Users/hrauxr/Projects/nxf-modules/tests/data/genome.fasta.gz', checkIfExists: true) + file(params.test_data['actinidia_chinensis']['genome']['genome_21_fasta_gz'], checkIfExists: true) ] """ } @@ -43,7 +86,7 @@ nextflow_workflow { then { assertAll( { assert workflow.success }, - { assert snapshot(workflow.out.versions).match("versions") } + { assert snapshot(workflow.out).match() } ) } } diff --git a/subworkflows/pfr/fasta_edta_lai/tests/main.nf.test.snap b/subworkflows/pfr/fasta_edta_lai/tests/main.nf.test.snap index 574acc9..2ab7da2 100644 --- a/subworkflows/pfr/fasta_edta_lai/tests/main.nf.test.snap +++ b/subworkflows/pfr/fasta_edta_lai/tests/main.nf.test.snap @@ -1,11 +1,87 @@ { - "versions": { + "actinidia_chinensis-genome_21_fasta_gz-stub": { "content": [ - [ - "versions.yml:md5,0d4bc49e94acb8995ca552d4e666e3ce", - "versions.yml:md5,754bb19f86be761d90c002a0af2faf1c" - ] + { + "0": [ + [ + { + "id": "test" + }, + "test.EDTA.TElib.fa:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.EDTA.TEanno.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + [ + { + "id": "test" + }, + "test.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + [ + { + "id": "test" + }, + "test.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "4": [ + "versions.yml:md5,0d4bc49e94acb8995ca552d4e666e3ce", + "versions.yml:md5,65666e975bdfd71978843ca963e84d0c", + "versions.yml:md5,754bb19f86be761d90c002a0af2faf1c" + ], + "lai_log": [ + [ + { + "id": "test" + }, + "test.LAI.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "lai_out": [ + [ + { + "id": "test" + }, + "test.LAI.out:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "te_anno_gff3": [ + [ + { + "id": "test" + }, + "test.EDTA.TEanno.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "te_lib_fasta": [ + [ + { + "id": "test" + }, + "test.EDTA.TElib.fa:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,0d4bc49e94acb8995ca552d4e666e3ce", + "versions.yml:md5,65666e975bdfd71978843ca963e84d0c", + "versions.yml:md5,754bb19f86be761d90c002a0af2faf1c" + ] + } ], - "timestamp": "2023-12-22T14:09:24.171934" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-20T18:05:46.667121" } } \ No newline at end of file diff --git a/subworkflows/pfr/fasta_edta_lai/tests/nextflow.config b/subworkflows/pfr/fasta_edta_lai/tests/nextflow.config new file mode 100644 index 0000000..1fa6315 --- /dev/null +++ b/subworkflows/pfr/fasta_edta_lai/tests/nextflow.config @@ -0,0 +1,5 @@ +process { + withName: EDTA_EDTA { + ext.args = '--anno 1' + } +} From 0cea739654e96cd7bd2afda7f3d2c082d6b1d0df Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 20 Mar 2024 19:11:19 +1300 Subject: [PATCH 03/40] Now tracking liftoff from nf-core/modules --- modules.json | 10 +- .../{pfr => nf-core}/liftoff/environment.yml | 0 modules/{pfr => nf-core}/liftoff/main.nf | 10 +- modules/{pfr => nf-core}/liftoff/meta.yml | 5 + .../liftoff/tests/main.nf.test | 25 +---- .../nf-core/liftoff/tests/main.nf.test.snap | 96 +++++++++++++++++++ modules/nf-core/liftoff/tests/nextflow.config | 5 + modules/nf-core/liftoff/tests/tags.yml | 2 + modules/pfr/liftoff/tests/main.nf.test.snap | 34 ------- modules/pfr/liftoff/tests/nextflow.config | 5 - modules/pfr/liftoff/tests/tags.yml | 2 - subworkflows/local/fasta_liftoff.nf | 5 +- 12 files changed, 127 insertions(+), 72 deletions(-) rename modules/{pfr => nf-core}/liftoff/environment.yml (100%) rename modules/{pfr => nf-core}/liftoff/main.nf (85%) rename modules/{pfr => nf-core}/liftoff/meta.yml (91%) rename modules/{pfr => nf-core}/liftoff/tests/main.nf.test (70%) create mode 100644 modules/nf-core/liftoff/tests/main.nf.test.snap create mode 100644 modules/nf-core/liftoff/tests/nextflow.config create mode 100644 modules/nf-core/liftoff/tests/tags.yml delete mode 100644 modules/pfr/liftoff/tests/main.nf.test.snap delete mode 100644 modules/pfr/liftoff/tests/nextflow.config delete mode 100644 modules/pfr/liftoff/tests/tags.yml diff --git a/modules.json b/modules.json index 361828c..adcf4de 100644 --- a/modules.json +++ b/modules.json @@ -20,11 +20,6 @@ "git_sha": "35468dbb1f35eb17a43d7e05544601c7c3f8cd90", "installed_by": ["fasta_edta_lai"] }, - "liftoff": { - "branch": "main", - "git_sha": "444b35f4e6285115f84d2bfce49fc0e6d8a2754e", - "installed_by": ["modules"] - }, "ltrretriever/lai": { "branch": "main", "git_sha": "154661d7c1769532ff7b5f11259644ec200dd47d", @@ -111,6 +106,11 @@ "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", "installed_by": ["modules"] }, + "liftoff": { + "branch": "master", + "git_sha": "8ce34a40589137b75b65dfe8bb334c9b94f1d6c8", + "installed_by": ["modules"] + }, "samtools/cat": { "branch": "master", "git_sha": "f4596fe0bdc096cf53ec4497e83defdb3a94ff62", diff --git a/modules/pfr/liftoff/environment.yml b/modules/nf-core/liftoff/environment.yml similarity index 100% rename from modules/pfr/liftoff/environment.yml rename to modules/nf-core/liftoff/environment.yml diff --git a/modules/pfr/liftoff/main.nf b/modules/nf-core/liftoff/main.nf similarity index 85% rename from modules/pfr/liftoff/main.nf rename to modules/nf-core/liftoff/main.nf index 317eca1..4db5da2 100644 --- a/modules/pfr/liftoff/main.nf +++ b/modules/nf-core/liftoff/main.nf @@ -11,6 +11,7 @@ process LIFTOFF { tuple val(meta), path(target_fa) path ref_fa, name: 'ref_assembly.fa' path ref_annotation + path ref_db output: tuple val(meta), path("${prefix}.gff3") , emit: gff3 @@ -22,11 +23,14 @@ process LIFTOFF { task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' - prefix = task.ext.prefix ?: "${meta.id}" + def args = task.ext.args ?: '' + def arg_g = ref_annotation ? "-g $ref_annotation" : '' + def arg_db = ref_db ? "-db $ref_db" : '' + prefix = task.ext.prefix ?: "${meta.id}" """ liftoff \\ - -g $ref_annotation \\ + $arg_g \\ + $arg_db \\ -p $task.cpus \\ -o "${prefix}.gff3" \\ -u "${prefix}.unmapped.txt" \\ diff --git a/modules/pfr/liftoff/meta.yml b/modules/nf-core/liftoff/meta.yml similarity index 91% rename from modules/pfr/liftoff/meta.yml rename to modules/nf-core/liftoff/meta.yml index 46b3c58..10e502c 100644 --- a/modules/pfr/liftoff/meta.yml +++ b/modules/nf-core/liftoff/meta.yml @@ -38,6 +38,11 @@ input: type: file description: Reference assembly annotations in gtf or gff3 format pattern: "*.{gtf,gff3}" + - ref_db: + type: file + description: | + Name of feature database; if not specified, the -g argument must + be provided and a database will be built automatically output: - meta: type: map diff --git a/modules/pfr/liftoff/tests/main.nf.test b/modules/nf-core/liftoff/tests/main.nf.test similarity index 70% rename from modules/pfr/liftoff/tests/main.nf.test rename to modules/nf-core/liftoff/tests/main.nf.test index 272c882..fc0f567 100644 --- a/modules/pfr/liftoff/tests/main.nf.test +++ b/modules/nf-core/liftoff/tests/main.nf.test @@ -9,6 +9,7 @@ nextflow_process { tag "modules_nfcore" tag "nf-core/gunzip" tag "liftoff" + tag "gunzip" test("homo_sapiens-genome_21_fasta-genome_1_fasta-genome_1_gtf") { @@ -38,6 +39,7 @@ nextflow_process { input[2] = [ file(params.test_data['homo_sapiens']['genome']['genome_1_gtf'], checkIfExists: true) ] + input[3] = [] """ } } @@ -48,16 +50,6 @@ nextflow_process { { assert snapshot(process.out.unmapped_txt).match("unmapped_txt") }, { assert file(process.out.gff3[0][1]).text.contains("chr21\tLiftoff\texon\t34608061\t34608118\t.\t+\t.") }, { assert file(process.out.polished_gff3[0][1]).text.contains("chr21\tLiftoff\texon\t34608061\t34608118\t.\t+\t.") }, - { - assert snapshot( - ( - [process.out.gff3[0][0].toString()] + // meta - process.out.gff3.collect { file(it[1]).getName() } + - process.out.polished_gff3.collect { file(it[1]).getName() } + - process.out.unmapped_txt.collect { file(it[1]).getName() } - ).sort() - ).match("homo_sapiens-genome_21_fasta-genome_1_fasta-genome_1_gtf-for_stub_match") - }, { assert snapshot(process.out.versions).match("versions") } ) } @@ -93,6 +85,7 @@ nextflow_process { input[2] = [ file(params.test_data['homo_sapiens']['genome']['genome_1_gtf'], checkIfExists: true) ] + input[3] = [] """ } } @@ -100,17 +93,7 @@ nextflow_process { then { assertAll( { assert process.success }, - { - assert snapshot( - ( - [process.out.gff3[0][0].toString()] + // meta - process.out.gff3.collect { file(it[1]).getName() } + - process.out.polished_gff3.collect { file(it[1]).getName() } + - process.out.unmapped_txt.collect { file(it[1]).getName() } - ).sort() - ).match("homo_sapiens-genome_21_fasta-genome_1_fasta-genome_1_gtf-for_stub_match") - }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out).match() } ) } diff --git a/modules/nf-core/liftoff/tests/main.nf.test.snap b/modules/nf-core/liftoff/tests/main.nf.test.snap new file mode 100644 index 0000000..f606446 --- /dev/null +++ b/modules/nf-core/liftoff/tests/main.nf.test.snap @@ -0,0 +1,96 @@ +{ + "unmapped_txt": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.unmapped.txt:md5,7391d10df6e15db356b084c9af5259e4" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2023-12-01T13:57:40.748507" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,205d9c609e7fe27d8199550d842bdce8" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2023-12-01T13:57:40.752414" + }, + "homo_sapiens-genome_21_fasta-genome_1_fasta-genome_1_gtf-stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.polished.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + [ + { + "id": "test" + }, + "test.unmapped.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,205d9c609e7fe27d8199550d842bdce8" + ], + "gff3": [ + [ + { + "id": "test" + }, + "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "polished_gff3": [ + [ + { + "id": "test" + }, + "test.polished.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "unmapped_txt": [ + [ + { + "id": "test" + }, + "test.unmapped.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,205d9c609e7fe27d8199550d842bdce8" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-19T09:15:25.661428" + } +} \ No newline at end of file diff --git a/modules/nf-core/liftoff/tests/nextflow.config b/modules/nf-core/liftoff/tests/nextflow.config new file mode 100644 index 0000000..f35ef80 --- /dev/null +++ b/modules/nf-core/liftoff/tests/nextflow.config @@ -0,0 +1,5 @@ +process { + withName: LIFTOFF { + ext.args = '-polish' + } +} diff --git a/modules/nf-core/liftoff/tests/tags.yml b/modules/nf-core/liftoff/tests/tags.yml new file mode 100644 index 0000000..4d0adb6 --- /dev/null +++ b/modules/nf-core/liftoff/tests/tags.yml @@ -0,0 +1,2 @@ +liftoff: + - "modules/nf-core/liftoff/**" diff --git a/modules/pfr/liftoff/tests/main.nf.test.snap b/modules/pfr/liftoff/tests/main.nf.test.snap deleted file mode 100644 index baa4d70..0000000 --- a/modules/pfr/liftoff/tests/main.nf.test.snap +++ /dev/null @@ -1,34 +0,0 @@ -{ - "unmapped_txt": { - "content": [ - [ - [ - { - "id": "test" - }, - "test.unmapped.txt:md5,7391d10df6e15db356b084c9af5259e4" - ] - ] - ], - "timestamp": "2023-12-01T13:57:40.748507" - }, - "versions": { - "content": [ - [ - "versions.yml:md5,205d9c609e7fe27d8199550d842bdce8" - ] - ], - "timestamp": "2023-12-01T13:57:40.752414" - }, - "homo_sapiens-genome_21_fasta-genome_1_fasta-genome_1_gtf-for_stub_match": { - "content": [ - [ - "test.gff3", - "test.polished.gff3", - "test.unmapped.txt", - "{id=test}" - ] - ], - "timestamp": "2023-12-21T15:20:04.816416" - } -} \ No newline at end of file diff --git a/modules/pfr/liftoff/tests/nextflow.config b/modules/pfr/liftoff/tests/nextflow.config deleted file mode 100644 index 06b9d76..0000000 --- a/modules/pfr/liftoff/tests/nextflow.config +++ /dev/null @@ -1,5 +0,0 @@ -process { - withName: LIFTOFF { - ext.args = '-exclude_partial -copies -polish -a 0.1 -s 0.1' - } -} diff --git a/modules/pfr/liftoff/tests/tags.yml b/modules/pfr/liftoff/tests/tags.yml deleted file mode 100644 index 4ae1fb0..0000000 --- a/modules/pfr/liftoff/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -liftoff: - - "modules/pfr/liftoff/**" diff --git a/subworkflows/local/fasta_liftoff.nf b/subworkflows/local/fasta_liftoff.nf index 4c59ba3..b69b7a3 100644 --- a/subworkflows/local/fasta_liftoff.nf +++ b/subworkflows/local/fasta_liftoff.nf @@ -1,7 +1,7 @@ include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/gunzip' include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/gunzip' include { GFFREAD } from '../../modules/nf-core/gffread' -include { LIFTOFF } from '../../modules/pfr/liftoff' +include { LIFTOFF } from '../../modules/nf-core/liftoff' workflow FASTA_LIFTOFF { take: @@ -83,7 +83,8 @@ workflow FASTA_LIFTOFF { LIFTOFF( ch_liftoff_inputs.map { meta, target_fa, ref_fa, ref_gff -> [ meta, target_fa ] }, ch_liftoff_inputs.map { meta, target_fa, ref_fa, ref_gff -> ref_fa }, - ch_liftoff_inputs.map { meta, target_fa, ref_fa, ref_gff -> ref_gff } + ch_liftoff_inputs.map { meta, target_fa, ref_fa, ref_gff -> ref_gff }, + [] ) ch_liftoff_gff3 = LIFTOFF.out.polished_gff3 From f1384ab9de7eb84894bacc114c8e348ac891a164 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 21 Mar 2024 15:57:30 +1300 Subject: [PATCH 04/40] Added before --- CHANGELOG.md | 3 ++- conf/manifest.config | 2 +- conf/modules.config | 8 ++++-- subworkflows/local/preprocess_rnaseq.nf | 36 +++++++++++++++++++------ 4 files changed, 37 insertions(+), 12 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 6961753..a25a3a7 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 0.2+dev - [20-Mar-2024] +## 0.2.0+dev - [21-Mar-2024] ### `Added` @@ -16,6 +16,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 7. Added versioning checking 8. Updated github workflow to use pre-commit instead of prettier and editorconfig check 9. Added central singularity cache dir for pfr config +10. Added `SORTMERNA_INDEX` before `SORTMERNA` ### `Fixed` diff --git a/conf/manifest.config b/conf/manifest.config index d61659f..93780a1 100644 --- a/conf/manifest.config +++ b/conf/manifest.config @@ -5,6 +5,6 @@ manifest { description = """A NextFlow pipeline for pan-genome annotation""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.4' - version = '0.2+dev' + version = '0.2.0+dev' doi = '' } diff --git a/conf/modules.config b/conf/modules.config index 09e9452..12a8ba2 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -90,8 +90,12 @@ if(!params.skip_fastp) { if (params.remove_ribo_rna) { process { - withName: 'SORTMERNA' { - ext.args = '--num_alignments 1 -v' + withName: SORTMERNA_INDEX { + ext.args = '--index 1' + } + + withName: SORTMERNA_READS { + ext.args = '--index 0 --num_alignments 1 -v' publishDir = [ [ path: { "${params.outdir}/sortmerna" }, diff --git a/subworkflows/local/preprocess_rnaseq.nf b/subworkflows/local/preprocess_rnaseq.nf index 81ff0d8..04231a0 100644 --- a/subworkflows/local/preprocess_rnaseq.nf +++ b/subworkflows/local/preprocess_rnaseq.nf @@ -1,5 +1,6 @@ include { CAT_FASTQ } from '../../modules/nf-core/cat/fastq' -include { SORTMERNA } from '../../modules/nf-core/sortmerna' +include { SORTMERNA as SORTMERNA_INDEX } from '../../modules/nf-core/sortmerna' +include { SORTMERNA as SORTMERNA_READS } from '../../modules/nf-core/sortmerna' include { EXTRACT_SAMPLES } from '../../subworkflows/local/extract_samples' include { FASTQ_FASTQC_UMITOOLS_FASTP } from '../../subworkflows/nf-core/fastq_fastqc_umitools_fastp' @@ -15,7 +16,7 @@ workflow PREPROCESS_RNASEQ { sortmerna_fastas // channel: [ [ fasta ] ] main: - ch_versions = Channel.empty() + ch_versions = Channel.empty() // SUBWORKFLOW: EXTRACT_SAMPLES EXTRACT_SAMPLES( @@ -81,17 +82,36 @@ workflow PREPROCESS_RNASEQ { ch_versions = ch_versions.mix(FASTQ_FASTQC_UMITOOLS_FASTP.out.versions.first()) - // MODULE: SORTMERNA - SORTMERNA( - remove_ribo_rna ? ch_trim_reads : Channel.empty(), - sortmerna_fastas.map { fastas -> [ [], fastas ] }, + + // MODULE: SORTMERNA as SORTMERNA_INDEX + SORTMERNA_INDEX( + [ [ id: 'idx' ], [] ], + sortmerna_fastas.map { fastas -> [ [ id: 'fastas' ], fastas ] }, [ [], [] ] ) + ch_versions = ch_versions.mix(SORTMERNA_INDEX.out.versions) + + // MODULE: SORTMERNA as SORTMERNA_READS + ch_sortmerna_inputs = remove_ribo_rna + ? ch_trim_reads + | combine( + sortmerna_fastas + | map { fastas -> [ [ id: 'fastas' ], fastas ] } + | join(SORTMERNA_INDEX.out.index) + ) + : Channel.empty() + + SORTMERNA_READS( + ch_sortmerna_inputs.map { meta, reads, meta2, fastas, idx -> [ meta, reads ] }, + ch_sortmerna_inputs.map { meta, reads, meta2, fastas, idx -> [ meta2, fastas ] }, + ch_sortmerna_inputs.map { meta, reads, meta2, fastas, idx -> [ meta2, idx ] } + ) + ch_emitted_reads = remove_ribo_rna - ? SORTMERNA.out.reads + ? SORTMERNA_READS.out.reads : ch_trim_reads - ch_versions = ch_versions.mix(SORTMERNA.out.versions.first()) + ch_versions = ch_versions.mix(SORTMERNA_READS.out.versions.first()) From 7ab82cb4b055a4064b5773d239503ee1ee58a1df Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 21 Mar 2024 21:53:14 +1300 Subject: [PATCH 05/40] Lightweight stub test and removed file.simpleName bug --- .github/workflows/test.yml | 10 +++---- CHANGELOG.md | 2 ++ conf/test_params.json | 25 +++++++++-------- conf/test_stub.config | 28 +++++++++++++++++++ modules/local/validate_params.nf | 18 ++++++++++++ subworkflows/local/fasta_liftoff.nf | 4 +-- .../RU01.20221115150135.chr1.pep.fasta.gz | 0 .../RU01.20221115150135.chr2.pep.fasta.gz | 0 ...Root1_162bp_C728RACXX_Lane1_R1.1k.fastq.gz | 0 ...Root1_162bp_C728RACXX_Lane1_R2.1k.fastq.gz | 0 ...Root2_156bp_C728RACXX_Lane1_R1.1k.fastq.gz | 0 ...Root2_156bp_C728RACXX_Lane1_R2.1k.fastq.gz | 0 ...cane3_165bp_C728RACXX_Lane1_R1.1k.fastq.gz | 0 ...cane3_165bp_C728RACXX_Lane1_R2.1k.fastq.gz | 0 tests/stub/fq/fastqsheet.csv | 4 +++ .../liftoff/RU01.20221115150135.chr1.gff3.gz | 0 .../liftoff/RU01.20221115150135.chr2.gff3.gz | 0 tests/stub/liftoff/Russell_V2a.chr1.fsa.gz | 0 tests/stub/liftoff/Russell_V2a.chr2.fsa.gz | 0 tests/stub/target/donghong.chr1.fsa.gz | 0 tests/stub/target/red5_v2p1_chr1.fasta.gz | 0 tests/stub/te_lib/donghong.TElib.fa.gz | 0 workflows/pangene.nf | 7 +++-- 23 files changed, 75 insertions(+), 23 deletions(-) create mode 100644 conf/test_stub.config create mode 100644 tests/stub/ext_prot/RU01.20221115150135.chr1.pep.fasta.gz create mode 100644 tests/stub/ext_prot/RU01.20221115150135.chr2.pep.fasta.gz create mode 100644 tests/stub/fq/1505KHS-0090_Root1_162bp_C728RACXX_Lane1_R1.1k.fastq.gz create mode 100644 tests/stub/fq/1505KHS-0090_Root1_162bp_C728RACXX_Lane1_R2.1k.fastq.gz create mode 100644 tests/stub/fq/1505KHS-0090_Root2_156bp_C728RACXX_Lane1_R1.1k.fastq.gz create mode 100644 tests/stub/fq/1505KHS-0090_Root2_156bp_C728RACXX_Lane1_R2.1k.fastq.gz create mode 100644 tests/stub/fq/1505KHS-0090_cane3_165bp_C728RACXX_Lane1_R1.1k.fastq.gz create mode 100644 tests/stub/fq/1505KHS-0090_cane3_165bp_C728RACXX_Lane1_R2.1k.fastq.gz create mode 100644 tests/stub/fq/fastqsheet.csv create mode 100644 tests/stub/liftoff/RU01.20221115150135.chr1.gff3.gz create mode 100644 tests/stub/liftoff/RU01.20221115150135.chr2.gff3.gz create mode 100644 tests/stub/liftoff/Russell_V2a.chr1.fsa.gz create mode 100644 tests/stub/liftoff/Russell_V2a.chr2.fsa.gz create mode 100644 tests/stub/target/donghong.chr1.fsa.gz create mode 100644 tests/stub/target/red5_v2p1_chr1.fasta.gz create mode 100644 tests/stub/te_lib/donghong.TElib.fa.gz diff --git a/.github/workflows/test.yml b/.github/workflows/test.yml index e1bacb8..65c2fa1 100644 --- a/.github/workflows/test.yml +++ b/.github/workflows/test.yml @@ -45,12 +45,10 @@ jobs: ssh-key: ${{ secrets.PANGENE_TEST_DEPLOY_KEY }} path: pangene-test - - uses: actions/setup-java@v3 - with: - distribution: "temurin" - java-version: "17" - - name: Setup Nextflow + - name: Install Nextflow uses: nf-core/setup-nextflow@v1 + with: + version: "23.04.4" - name: Run stub-test run: | @@ -59,7 +57,7 @@ jobs: -profile local,docker \ -resume \ -stub \ - -params-file conf/test_params.json + -c conf/test_stub.config confirm-pass: runs-on: ubuntu-latest diff --git a/CHANGELOG.md b/CHANGELOG.md index a25a3a7..a60ce7c 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -17,6 +17,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 8. Updated github workflow to use pre-commit instead of prettier and editorconfig check 9. Added central singularity cache dir for pfr config 10. Added `SORTMERNA_INDEX` before `SORTMERNA` +11. Fixed sample contamination bug introduced by `file.simpleName` +12. Now using empty files for stub testing in CI ### `Fixed` diff --git a/conf/test_params.json b/conf/test_params.json index 0dc25f8..0bb8196 100644 --- a/conf/test_params.json +++ b/conf/test_params.json @@ -1,28 +1,29 @@ { "target_assemblies": [ - ["red5_v2p1", "pangene-test/target/red5_v2p1_chr1_1200k.fasta.gz"], - ["donghong", "pangene-test/target/donghong_chr1_600k.fsa.gz"] + ["red5_v2p1", "pangene-test/target/red5_v2p1_chr1.fasta.gz"], + ["donghong", "pangene-test/target/donghong.chr1.fsa.gz"] ], - "te_libraries": [["donghong", "pangene-test/te_lib/donghong.TElib.fa.gz"]], + "te_libraries": [ + ["red5_v2p1", "pangene-test/te_lib/red5_v2p1.fa.gz"], + ["donghong", "pangene-test/te_lib/donghong.TElib.fa.gz"] + ], - "samplesheet": "pangene-test/samplesheet/samplesheet.csv", + "samplesheet": "pangene-test/fq/fastqsheet.csv", "remove_ribo_rna": true, "ribo_database_manifest": "assets/rrna-db-test.txt", "external_protein_fastas": [ - "pangene-test/ext_prot/RU01_20221115150135_chr1_600k.pep.fasta.gz", - "pangene-test/ext_prot/RU01_20221115150135_chr2_600k.pep.fasta.gz" + "pangene-test/ext_prot/RU01.20221115150135.chr1.pep.fasta.gz", + "pangene-test/ext_prot/RU01.20221115150135.chr2.pep.fasta.gz" ], - "braker_extra_args": "--testMode --species=arabidopsis --useexisting", - "liftoff_xref_annotations": [ - ["pangene-test/liftoff/Russell_V2a_chr1_600k.fsa.gz", "pangene-test/liftoff/Russell_V2a_chr1_600k.gff3.gz"], - ["pangene-test/liftoff/TAIR10_chr1_600k.fas.gz", "pangene-test/liftoff/TAIR10_chr1_600k.gff3.gz"] + ["pangene-test/liftoff/Russell_V2a.chr1.fsa.gz", "pangene-test/liftoff/RU01.20221115150135.chr1.gff3.gz"], + ["pangene-test/liftoff/Russell_V2a.chr2.fsa.gz", "pangene-test/liftoff/RU01.20221115150135.chr2.gff3.gz"] ], - "max_cpus": 2, - "max_memory": "3.GB" + "max_cpus": 8, + "max_memory": "32.GB" } diff --git a/conf/test_stub.config b/conf/test_stub.config new file mode 100644 index 0000000..d90f41e --- /dev/null +++ b/conf/test_stub.config @@ -0,0 +1,28 @@ +params { + target_assemblies = [ + ["red5_v2p1", "tests/stub/target/red5_v2p1_chr1.fasta.gz"], + ["donghong", "tests/stub/target/donghong.chr1.fsa.gz"] + ] + + te_libraries = [ + ["donghong", "tests/stub/te_lib/donghong.TElib.fa.gz"] + ] + + samplesheet = "tests/stub/fq/fastqsheet.csv" + + remove_ribo_rna = true + ribo_database_manifest = "assets/rrna-db-test.txt" + + external_protein_fastas = [ + "tests/stub/ext_prot/RU01.20221115150135.chr1.pep.fasta.gz", + "tests/stub/ext_prot/RU01.20221115150135.chr2.pep.fasta.gz" + ] + + liftoff_xref_annotations = [ + ["tests/stub/liftoff/Russell_V2a.chr1.fsa.gz", "tests/stub/liftoff/RU01.20221115150135.chr1.gff3.gz"], + ["tests/stub/liftoff/Russell_V2a.chr2.fsa.gz", "tests/stub/liftoff/RU01.20221115150135.chr2.gff3.gz"] + ] + + max_cpus = 2 + max_memory = 3.GB +} diff --git a/modules/local/validate_params.nf b/modules/local/validate_params.nf index 460ce80..94d200e 100644 --- a/modules/local/validate_params.nf +++ b/modules/local/validate_params.nf @@ -102,3 +102,21 @@ def validateLiftoffXrefs(params) { def isNotListOfLists(thisOne, subListSize) { return (!(thisOne instanceof List) || thisOne.isEmpty() || thisOne.any { !(it instanceof List) || it.size() != subListSize }) } + +def id_from_file_name(file_name) { + + def trial = ( file_name + ).replaceFirst( + /\.f(ast)?q$/, '' + ).replaceFirst( + /\.f(asta|sa|a|as|aa)?$/, '' + ).replaceFirst( + /\.gff(3)?$/, '' + ).replaceFirst( + /\.gz$/, '' + ) + + if ( trial == file_name ) { return file_name } + + return id_from_file_name ( trial ) +} diff --git a/subworkflows/local/fasta_liftoff.nf b/subworkflows/local/fasta_liftoff.nf index b69b7a3..a842944 100644 --- a/subworkflows/local/fasta_liftoff.nf +++ b/subworkflows/local/fasta_liftoff.nf @@ -47,13 +47,13 @@ workflow FASTA_LIFTOFF { // MODULE: GFFREAD ch_gffread_inputs = ch_xref_gunzip_gff | map { meta, gff -> - [ gff.getSimpleName(), meta, gff ] + [ gff.baseName, meta, gff ] } // For meta insertion later, remove when GFFREAD has meta GFFREAD ( ch_gffread_inputs.map { name, meta, gff -> gff } ) ch_gffread_gff = GFFREAD.out.gffread_gff - | map { gff -> [ gff.getSimpleName(), gff ] } + | map { gff -> [ gff.baseName - '.gffread', gff ] } | join(ch_gffread_inputs) | map { fid, gffread_gff, meta, gff -> [ meta, gffread_gff ] } // meta insertion diff --git a/tests/stub/ext_prot/RU01.20221115150135.chr1.pep.fasta.gz b/tests/stub/ext_prot/RU01.20221115150135.chr1.pep.fasta.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/ext_prot/RU01.20221115150135.chr2.pep.fasta.gz b/tests/stub/ext_prot/RU01.20221115150135.chr2.pep.fasta.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/fq/1505KHS-0090_Root1_162bp_C728RACXX_Lane1_R1.1k.fastq.gz b/tests/stub/fq/1505KHS-0090_Root1_162bp_C728RACXX_Lane1_R1.1k.fastq.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/fq/1505KHS-0090_Root1_162bp_C728RACXX_Lane1_R2.1k.fastq.gz b/tests/stub/fq/1505KHS-0090_Root1_162bp_C728RACXX_Lane1_R2.1k.fastq.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/fq/1505KHS-0090_Root2_156bp_C728RACXX_Lane1_R1.1k.fastq.gz b/tests/stub/fq/1505KHS-0090_Root2_156bp_C728RACXX_Lane1_R1.1k.fastq.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/fq/1505KHS-0090_Root2_156bp_C728RACXX_Lane1_R2.1k.fastq.gz b/tests/stub/fq/1505KHS-0090_Root2_156bp_C728RACXX_Lane1_R2.1k.fastq.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/fq/1505KHS-0090_cane3_165bp_C728RACXX_Lane1_R1.1k.fastq.gz b/tests/stub/fq/1505KHS-0090_cane3_165bp_C728RACXX_Lane1_R1.1k.fastq.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/fq/1505KHS-0090_cane3_165bp_C728RACXX_Lane1_R2.1k.fastq.gz b/tests/stub/fq/1505KHS-0090_cane3_165bp_C728RACXX_Lane1_R2.1k.fastq.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/fq/fastqsheet.csv b/tests/stub/fq/fastqsheet.csv new file mode 100644 index 0000000..7c35f1e --- /dev/null +++ b/tests/stub/fq/fastqsheet.csv @@ -0,0 +1,4 @@ +sample,fastq_1,fastq_2,target_assemblies +Root1,1505KHS-0090_Root1_162bp_C728RACXX_Lane1_R1.1k.fastq.gz,1505KHS-0090_Root1_162bp_C728RACXX_Lane1_R2.1k.fastq.gz,red5_v2p1 +Root1,1505KHS-0090_Root2_156bp_C728RACXX_Lane1_R1.1k.fastq.gz,1505KHS-0090_Root2_156bp_C728RACXX_Lane1_R2.1k.fastq.gz,red5_v2p1 +cane3,1505KHS-0090_cane3_165bp_C728RACXX_Lane1_R1.1k.fastq.gz,1505KHS-0090_cane3_165bp_C728RACXX_Lane1_R2.1k.fastq.gz,red5_v2p1;donghong diff --git a/tests/stub/liftoff/RU01.20221115150135.chr1.gff3.gz b/tests/stub/liftoff/RU01.20221115150135.chr1.gff3.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/liftoff/RU01.20221115150135.chr2.gff3.gz b/tests/stub/liftoff/RU01.20221115150135.chr2.gff3.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/liftoff/Russell_V2a.chr1.fsa.gz b/tests/stub/liftoff/Russell_V2a.chr1.fsa.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/liftoff/Russell_V2a.chr2.fsa.gz b/tests/stub/liftoff/Russell_V2a.chr2.fsa.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/target/donghong.chr1.fsa.gz b/tests/stub/target/donghong.chr1.fsa.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/target/red5_v2p1_chr1.fasta.gz b/tests/stub/target/red5_v2p1_chr1.fasta.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/te_lib/donghong.TElib.fa.gz b/tests/stub/te_lib/donghong.TElib.fa.gz new file mode 100644 index 0000000..e69de29 diff --git a/workflows/pangene.nf b/workflows/pangene.nf index 8512ff9..b37199c 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -1,4 +1,5 @@ include { validateParams } from '../modules/local/validate_params' +include { id_from_file_name } from '../modules/local/validate_params' include { PREPARE_ASSEMBLY } from '../subworkflows/local/prepare_assembly' include { PREPROCESS_RNASEQ } from '../subworkflows/local/preprocess_rnaseq' include { ALIGN_RNASEQ } from '../subworkflows/local/align_rnaseq' @@ -46,7 +47,7 @@ workflow PANGENE { ? Channel.fromList(params.external_protein_fastas) | map { filePath -> def fileHandle = file(filePath, checkIfExists: true) - [ [ id: fileHandle.getSimpleName() ], fileHandle] + [ [ id: id_from_file_name( fileHandle.baseName ) ], fileHandle] } : Channel.empty() @@ -55,8 +56,8 @@ workflow PANGENE { | multiMap { fasta, gff -> def fastaFile = file(fasta, checkIfExists:true) - fasta: [ [ id: fastaFile.getSimpleName() ], fastaFile ] - gff: [ [ id: fastaFile.getSimpleName() ], file(gff, checkIfExists:true) ] + fasta: [ [ id: id_from_file_name( fastaFile.baseName ) ], fastaFile ] + gff: [ [ id: id_from_file_name( fastaFile.baseName ) ], file(gff, checkIfExists:true) ] } : Channel.empty() From 458dedf55ce20f6b9e7b04c23bb9ef13cc474548 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 25 Mar 2024 10:53:11 +1300 Subject: [PATCH 06/40] Now using 1 round for augustus training for test data --- conf/test_params.json | 7 +------ 1 file changed, 1 insertion(+), 6 deletions(-) diff --git a/conf/test_params.json b/conf/test_params.json index 0bb8196..48e0ea1 100644 --- a/conf/test_params.json +++ b/conf/test_params.json @@ -3,27 +3,22 @@ ["red5_v2p1", "pangene-test/target/red5_v2p1_chr1.fasta.gz"], ["donghong", "pangene-test/target/donghong.chr1.fsa.gz"] ], - "te_libraries": [ ["red5_v2p1", "pangene-test/te_lib/red5_v2p1.fa.gz"], ["donghong", "pangene-test/te_lib/donghong.TElib.fa.gz"] ], - "samplesheet": "pangene-test/fq/fastqsheet.csv", - "remove_ribo_rna": true, "ribo_database_manifest": "assets/rrna-db-test.txt", - "external_protein_fastas": [ "pangene-test/ext_prot/RU01.20221115150135.chr1.pep.fasta.gz", "pangene-test/ext_prot/RU01.20221115150135.chr2.pep.fasta.gz" ], - + "braker_extra_args": "--rounds=1", "liftoff_xref_annotations": [ ["pangene-test/liftoff/Russell_V2a.chr1.fsa.gz", "pangene-test/liftoff/RU01.20221115150135.chr1.gff3.gz"], ["pangene-test/liftoff/Russell_V2a.chr2.fsa.gz", "pangene-test/liftoff/RU01.20221115150135.chr2.gff3.gz"] ], - "max_cpus": 8, "max_memory": "32.GB" } From 5dd99c6091214bb7930434e18f9f9bd97d42b5c2 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 25 Mar 2024 15:14:21 +1300 Subject: [PATCH 07/40] Now braker can be skipped by providing braker files --- conf/test_params.json | 14 +++- modules/local/validate_params.nf | 11 ++- subworkflows/local/align_rnaseq.nf | 2 +- subworkflows/local/extract_samples.nf | 27 ++++++-- subworkflows/local/fasta_braker3.nf | 65 ++++++++++++++++++ subworkflows/local/file_gunzip.nf | 20 ++++++ subworkflows/local/prepare_assembly.nf | 32 ++++++++- subworkflows/local/preprocess_rnaseq.nf | 4 +- workflows/pangene.nf | 89 +++++++++++++++---------- 9 files changed, 212 insertions(+), 52 deletions(-) create mode 100644 subworkflows/local/fasta_braker3.nf create mode 100644 subworkflows/local/file_gunzip.nf diff --git a/conf/test_params.json b/conf/test_params.json index 48e0ea1..8466dfa 100644 --- a/conf/test_params.json +++ b/conf/test_params.json @@ -1,7 +1,17 @@ { "target_assemblies": [ - ["red5_v2p1", "pangene-test/target/red5_v2p1_chr1.fasta.gz"], - ["donghong", "pangene-test/target/donghong.chr1.fsa.gz"] + [ + "red5_v2p1", + "pangene-test/target/red5_v2p1_chr1.fasta.gz", + "pangene-test/braker/red5_v2p1.gff3.gz", + "pangene-test/braker/red5_v2p1.hints.gff.gz" + ], + [ + "donghong", + "pangene-test/target/donghong.chr1.fsa.gz", + "pangene-test/braker/donghong.gff3.gz", + "pangene-test/braker/donghong.hints.gff.gz" + ] ], "te_libraries": [ ["red5_v2p1", "pangene-test/te_lib/red5_v2p1.fa.gz"], diff --git a/modules/local/validate_params.nf b/modules/local/validate_params.nf index 94d200e..59102ed 100644 --- a/modules/local/validate_params.nf +++ b/modules/local/validate_params.nf @@ -20,8 +20,8 @@ def validateParams(params) { def validateFastaTags(params) { def listOfFastaTuples = params["target_assemblies"] - if (isNotListOfLists(listOfFastaTuples, 2)) { - error 'Error: target_assemblies must be a list of sublists, with each sublist containing 2 elements' + if ( isNotListOfLists(listOfFastaTuples, [ 2, 4 ] ) ) { + error 'Error: target_assemblies must be a list of sublists, with each sublist containing 2 or 4 elements' } def fastaTags = listOfFastaTuples.collect { it[0] } @@ -100,7 +100,12 @@ def validateLiftoffXrefs(params) { } def isNotListOfLists(thisOne, subListSize) { - return (!(thisOne instanceof List) || thisOne.isEmpty() || thisOne.any { !(it instanceof List) || it.size() != subListSize }) + + if ( subListSize instanceof Integer ) { + return (!(thisOne instanceof List) || thisOne.isEmpty() || thisOne.any { !(it instanceof List) || it.size() != subListSize }) + } + + return (!(thisOne instanceof List) || thisOne.isEmpty() || thisOne.any { !( it instanceof List ) || !( subListSize.contains( it.size() ) ) }) } def id_from_file_name(file_name) { diff --git a/subworkflows/local/align_rnaseq.nf b/subworkflows/local/align_rnaseq.nf index 05cd2fa..94d8b8d 100644 --- a/subworkflows/local/align_rnaseq.nf +++ b/subworkflows/local/align_rnaseq.nf @@ -71,6 +71,6 @@ workflow ALIGN_RNASEQ { ch_versions = ch_versions.mix(SAMTOOLS_CAT.out.versions.first()) emit: - bam = ch_samtools_bam // channel: [ [ id, single_end, target_assembly ], [ bam ] ] + bam = ch_samtools_bam // channel: [ [ id: target_assembly, single_end ], [ bam ] ] versions = ch_versions // channel: [ versions.yml ] } diff --git a/subworkflows/local/extract_samples.nf b/subworkflows/local/extract_samples.nf index 947c0b7..8ddb7ae 100644 --- a/subworkflows/local/extract_samples.nf +++ b/subworkflows/local/extract_samples.nf @@ -17,6 +17,7 @@ workflow EXTRACT_SAMPLES { take: samplesheet // file: /path/to/samplesheet.csv permissible_target_assemblies // val: assembly_a,assembly_b + exclude_assemblies // channel: val(assembly_x,assembly_y) main: SAMPLESHEET_CHECK ( samplesheet, permissible_target_assemblies ) @@ -28,13 +29,27 @@ workflow EXTRACT_SAMPLES { } | set { ch_reads } - reads = ch_reads.map { meta, fastq -> [[id:meta.id, single_end:meta.single_end], fastq]} + reads = ch_reads + | combine( exclude_assemblies ) + | map { meta, fastq, ex_assemblies -> + def ex_list = ex_assemblies.split(",") - ch_reads - | flatMap { meta, fastq -> - meta.target_assemblies.collect { assembly -> [[id:meta.id, single_end:meta.single_end], assembly] } - } - | set { assemblies } + if ( !( meta.target_assemblies.every { ex_list.contains( it ) } ) ) { + [ [ id:meta.id, single_end:meta.single_end ], fastq ] + } + } + + + assemblies = ch_reads + | combine( exclude_assemblies ) + | flatMap { meta, fastq, ex_assemblies -> + def ex_list = ex_assemblies.split(",") + + meta + .target_assemblies + .collect { assembly -> [ [ id:meta.id, single_end:meta.single_end ], assembly ] } + .findAll { _meta, assembly -> !( ex_list.contains( assembly ) ) } + } emit: reads // channel: [ val(meta), [ reads ] ] diff --git a/subworkflows/local/fasta_braker3.nf b/subworkflows/local/fasta_braker3.nf new file mode 100644 index 0000000..0ae0628 --- /dev/null +++ b/subworkflows/local/fasta_braker3.nf @@ -0,0 +1,65 @@ +include { BRAKER3 } from '../../modules/kherronism/braker3' +include { FILE_GUNZIP as BRAKER_GFF3_GUNZIP } from '../../subworkflows/local/file_gunzip' +include { FILE_GUNZIP as BRAKER_HINTS_GUNZIP } from '../../subworkflows/local/file_gunzip' + +workflow FASTA_BRAKER3 { + take: + ch_masked_target_assembly // channel: [ meta, fasta ]; meta ~ [ id: traget_assembly ] + ch_braker_ex_asm_str // channel: val(assembly_x,assembly_y) + ch_rnaseq_bam // channel: [ meta, bam ] + ch_ext_prots_fasta // channel: [ meta2, fasta ]; meta2 ~ [ id: ext_protein_seqs ] + ch_braker_annotation // channel: [ meta, gff3, hints.gff ] + + main: + ch_versions = Channel.empty() + + + ch_braker_inputs = ch_masked_target_assembly + | combine( ch_braker_ex_asm_str ) + | filter { meta, fasta, ex_str -> !( ex_str.split(",").contains( meta.id ) ) } + | map { meta, fasta, ex_str -> + [ meta, fasta ] + } + | join(ch_rnaseq_bam, remainder: true) + | combine( + ch_ext_prots_fasta.map { meta, fasta -> fasta }.ifEmpty(null) + ) + | map { meta, fasta, bam, prots -> [ meta, fasta, bam ?: [], prots ?: [] ] } + + def rnaseq_sets_dirs = [] + def rnaseq_sets_ids = [] + def hintsfile = [] + + // MODULE: BRAKER3 + BRAKER3( + ch_braker_inputs.map { meta, fasta, bam, prots -> [meta, fasta] }, + ch_braker_inputs.map { meta, fasta, bam, prots -> bam }, + rnaseq_sets_dirs, + rnaseq_sets_ids, + ch_braker_inputs.map { meta, fasta, bam, prots -> prots }, + hintsfile + ) + + ch_braker_gff3 = BRAKER3.out.gff3 + | mix( ch_braker_annotation.map { meta, gff3, hints -> [ meta, gff3 ] } ) + ch_braker_hints = BRAKER3.out.hintsfile + | mix( ch_braker_annotation.map { meta, gff3, hints -> [ meta, hints ] } ) + ch_versions = ch_versions.mix(BRAKER3.out.versions.first()) + + // WORKFLOW: FILE_GUNZIP as BRAKER_GFF3_GUNZIP + BRAKER_GFF3_GUNZIP ( ch_braker_gff3 ) + + ch_braker_gff3 = BRAKER_GFF3_GUNZIP.out.gunzip + ch_versions = ch_versions.mix(BRAKER_GFF3_GUNZIP.out.versions) + + // WORKFLOW: FILE_GUNZIP as BRAKER_HINTS_GUNZIP + BRAKER_HINTS_GUNZIP ( ch_braker_hints ) + + ch_braker_hints = BRAKER_GFF3_GUNZIP.out.gunzip + ch_versions = ch_versions.mix(BRAKER_HINTS_GUNZIP.out.versions) + + emit: + braker_gff3 = ch_braker_gff3 // [ meta, gff3 ] + braker_hints = ch_braker_hints // [ meta, hints.gff ] + versions = ch_versions // [ versions.yml ] +} diff --git a/subworkflows/local/file_gunzip.nf b/subworkflows/local/file_gunzip.nf new file mode 100644 index 0000000..30f3368 --- /dev/null +++ b/subworkflows/local/file_gunzip.nf @@ -0,0 +1,20 @@ +include { GUNZIP } from '../../modules/nf-core/gunzip' + +workflow FILE_GUNZIP { + take: + ch_input // channel [ meta, archive ] + + main: + ch_input_branch = ch_input + | branch { meta, archive -> + gz: "$archive".endsWith('.gz') + rest: ! "$archive".endsWith('.gz') + } + + // MODULE: GUNZIP + GUNZIP ( ch_input_branch.gz ) + + emit: + versions = GUNZIP.out.versions.first() + gunzip = GUNZIP.out.gunzip.mix( ch_input_branch.rest ) +} diff --git a/subworkflows/local/prepare_assembly.nf b/subworkflows/local/prepare_assembly.nf index d18f5ce..f0c9d7f 100644 --- a/subworkflows/local/prepare_assembly.nf +++ b/subworkflows/local/prepare_assembly.nf @@ -13,6 +13,7 @@ workflow PREPARE_ASSEMBLY { target_assembly // channel: [ meta, fasta ] te_library // channel: [ meta, fasta ] repeat_annotator // val(String), 'repeatmodeler' or 'edta' + exclude_assemblies // channel: val(assembly_x,assembly_y) main: ch_versions = Channel.empty() @@ -74,6 +75,14 @@ workflow PREPARE_ASSEMBLY { ch_edta_inputs = repeat_annotator != 'edta' ? Channel.empty() : ch_annotator_inputs + | combine( exclude_assemblies ) + | map { meta, fasta, ex_assemblies -> + def ex_list = ex_assemblies.split(",") + + if ( !( ex_list.contains( meta.id ) ) ) { + [ meta, fasta ] + } + } FASTA_EDTA_LAI( ch_edta_inputs, @@ -87,6 +96,14 @@ workflow PREPARE_ASSEMBLY { ch_repeatmodeler_inputs = repeat_annotator != 'repeatmodeler' ? Channel.empty() : ch_annotator_inputs + | combine( exclude_assemblies ) + | map { meta, fasta, ex_assemblies -> + def ex_list = ex_assemblies.split(",") + + if ( !( ex_list.contains( meta.id ) ) ) { + [ meta, fasta ] + } + } REPEATMODELER_BUILDDATABASE ( ch_repeatmodeler_inputs ) @@ -113,9 +130,20 @@ workflow PREPARE_ASSEMBLY { ch_versions = ch_versions.mix(REPEATMASKER.out.versions.first()) // MODULE: STAR_GENOMEGENERATE + ch_genomegenerate_inputs = ch_validated_assembly + | combine( exclude_assemblies ) + | map { meta, fasta, ex_assemblies -> + def ex_list = ex_assemblies.split(",") + + if ( !( ex_list.contains( meta.id ) ) ) { + [ meta, fasta ] + } + } + + STAR_GENOMEGENERATE( - ch_validated_assembly, - ch_validated_assembly.map { meta, fasta -> [ [], [] ] } + ch_genomegenerate_inputs, + ch_genomegenerate_inputs.map { meta, fasta -> [ [], [] ] } ) ch_assembly_index = STAR_GENOMEGENERATE.out.index diff --git a/subworkflows/local/preprocess_rnaseq.nf b/subworkflows/local/preprocess_rnaseq.nf index 04231a0..8557c0d 100644 --- a/subworkflows/local/preprocess_rnaseq.nf +++ b/subworkflows/local/preprocess_rnaseq.nf @@ -8,6 +8,7 @@ workflow PREPROCESS_RNASEQ { take: samplesheet // path: csv permissible_assemblies // val: assembly_a,assembly_b + exclude_assemblies // channel: val(assembly_x,assembly_y) skip_fastqc // val: true|false skip_fastp // val: true|false save_trimmed // val: true|false @@ -21,7 +22,8 @@ workflow PREPROCESS_RNASEQ { // SUBWORKFLOW: EXTRACT_SAMPLES EXTRACT_SAMPLES( samplesheet, - permissible_assemblies + permissible_assemblies, + exclude_assemblies ) ch_fastq = EXTRACT_SAMPLES.out.reads diff --git a/workflows/pangene.nf b/workflows/pangene.nf index b37199c..98c6dc1 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -1,12 +1,12 @@ -include { validateParams } from '../modules/local/validate_params' -include { id_from_file_name } from '../modules/local/validate_params' -include { PREPARE_ASSEMBLY } from '../subworkflows/local/prepare_assembly' -include { PREPROCESS_RNASEQ } from '../subworkflows/local/preprocess_rnaseq' -include { ALIGN_RNASEQ } from '../subworkflows/local/align_rnaseq' -include { PREPARE_EXT_PROTS } from '../subworkflows/local/prepare_ext_prots' -include { BRAKER3 } from '../modules/kherronism/braker3' -include { FASTA_LIFTOFF } from '../subworkflows/local/fasta_liftoff' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions' +include { validateParams } from '../modules/local/validate_params' +include { id_from_file_name } from '../modules/local/validate_params' +include { PREPARE_ASSEMBLY } from '../subworkflows/local/prepare_assembly' +include { PREPROCESS_RNASEQ } from '../subworkflows/local/preprocess_rnaseq' +include { ALIGN_RNASEQ } from '../subworkflows/local/align_rnaseq' +include { PREPARE_EXT_PROTS } from '../subworkflows/local/prepare_ext_prots' +include { FASTA_BRAKER3 } from '../subworkflows/local/fasta_braker3' +include { FASTA_LIFTOFF } from '../subworkflows/local/fasta_liftoff' +include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions' validateParams(params) @@ -15,13 +15,37 @@ workflow PANGENE { ch_versions = Channel.empty() ch_target_assembly = Channel.fromList(params.target_assemblies) - | map { tag, filePath -> - [ [ id: tag ], file(filePath, checkIfExists: true) ] + | map { it -> + def tag = it[0] + def fasta = it[1] + + [ [ id: tag ], file(fasta, checkIfExists: true) ] + } + + ch_braker_annotation = Channel.fromList(params.target_assemblies) + | map { it -> + if ( it.size() == 4 ) { + def tag = it[0] + def braker_gff3 = it[2] + def hints_gff = it[3] + + [ + [ id: tag ], + file(braker_gff3, checkIfExists: true), + file(hints_gff, checkIfExists: true) + ] + } } + ch_braker_ex_asm_str = ch_braker_annotation + | map { meta, braker_gff3, hints_gff -> meta.id } + | collect + | map { it.join(",") } + | ifEmpty( "" ) + ch_te_library = Channel.fromList(params.te_libraries) - | map { tag, filePath -> - [ [ id:tag ], file(filePath, checkIfExists: true) ] + | map { tag, fasta -> + [ [ id:tag ], file(fasta, checkIfExists: true) ] } ch_samplesheet = params.samplesheet @@ -30,7 +54,7 @@ workflow PANGENE { ch_tar_assm_str = Channel.of( params.target_assemblies - .collect { tag, fastaPath -> tag.strip() }.join(",") + .collect { it -> it[0].strip() }.join(",") // it[0] = tag ) ch_ribo_db = params.remove_ribo_rna @@ -68,7 +92,8 @@ workflow PANGENE { PREPARE_ASSEMBLY( ch_target_assembly, ch_te_library, - params.repeat_annotator + params.repeat_annotator, + ch_braker_ex_asm_str ) ch_valid_target_assembly = PREPARE_ASSEMBLY.out.target_assemby @@ -80,6 +105,7 @@ workflow PANGENE { PREPROCESS_RNASEQ( ch_samplesheet, ch_tar_assm_str, + ch_braker_ex_asm_str, params.skip_fastqc, params.skip_fastp, params.save_trimmed, @@ -96,7 +122,7 @@ workflow PANGENE { ALIGN_RNASEQ( ch_reads_target, ch_trim_reads, - ch_target_assemby_index + ch_target_assemby_index, ) ch_rnaseq_bam = ALIGN_RNASEQ.out.bam @@ -110,29 +136,18 @@ workflow PANGENE { ch_ext_prots_fasta = PREPARE_EXT_PROTS.out.ext_prots_fasta ch_versions = ch_versions.mix(PREPARE_EXT_PROTS.out.versions) - // MODULE: BRAKER3 - ch_braker_inputs = ch_masked_target_assembly - | join(ch_rnaseq_bam, remainder: true) - | combine( - ch_ext_prots_fasta.map { meta, filePath -> filePath }.ifEmpty(null) - ) - | map { meta, fasta, bam, prots -> [ meta, fasta, bam ?: [], prots ?: [] ] } - - def rnaseq_sets_dirs = [] - def rnaseq_sets_ids = [] - def hintsfile = [] - - BRAKER3( - ch_braker_inputs.map { meta, fasta, bam, prots -> [meta, fasta] }, - ch_braker_inputs.map { meta, fasta, bam, prots -> bam }, - rnaseq_sets_dirs, - rnaseq_sets_ids, - ch_braker_inputs.map { meta, fasta, bam, prots -> prots }, - hintsfile + // SUBWORKFLOW: FASTA_BRAKER3 + FASTA_BRAKER3( + ch_masked_target_assembly, + ch_braker_ex_asm_str, + ch_rnaseq_bam, + ch_ext_prots_fasta, + ch_braker_annotation ) - ch_braker_gff3 = BRAKER3.out.gff3 - ch_versions = ch_versions.mix(BRAKER3.out.versions.first()) + ch_braker_gff3 = FASTA_BRAKER3.out.braker_gff3 + ch_braker_hints = FASTA_BRAKER3.out.braker_hints + ch_versions = ch_versions.mix(FASTA_BRAKER3.out.versions) // SUBWORKFLOW: FASTA_LIFTOFF FASTA_LIFTOFF( From 4f6feae059326f9bd9de5d0c30f47487931dd8bf Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 25 Mar 2024 16:58:53 +1300 Subject: [PATCH 08/40] Added gffcompare to merge liftoff annotations --- CHANGELOG.md | 4 +- conf/modules.config | 16 +++- modules.json | 5 ++ modules/nf-core/gffcompare/environment.yml | 7 ++ modules/nf-core/gffcompare/main.nf | 63 +++++++++++++++ modules/nf-core/gffcompare/meta.yml | 91 ++++++++++++++++++++++ subworkflows/local/fasta_liftoff.nf | 53 ++++++++++--- 7 files changed, 228 insertions(+), 11 deletions(-) create mode 100644 modules/nf-core/gffcompare/environment.yml create mode 100644 modules/nf-core/gffcompare/main.nf create mode 100644 modules/nf-core/gffcompare/meta.yml diff --git a/CHANGELOG.md b/CHANGELOG.md index a60ce7c..12fefcc 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 0.2.0+dev - [21-Mar-2024] +## 0.2.0+dev - [25-Mar-2024] ### `Added` @@ -19,6 +19,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 10. Added `SORTMERNA_INDEX` before `SORTMERNA` 11. Fixed sample contamination bug introduced by `file.simpleName` 12. Now using empty files for stub testing in CI +13. Now BRAKER can be skipped by including BRAKER outputs from previous runs in the `target_assemblies` param +14. Added `gffcompare` to merge liftoff annotations ### `Fixed` diff --git a/conf/modules.config b/conf/modules.config index 12a8ba2..b5dc000 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -172,9 +172,23 @@ if(params.liftoff_xref_annotations) { ] } - withName: GFFREAD { + withName: GFFREAD_BEFORE_LIFTOFF { ext.args = '--no-pseudo --keep-genes' } + + withName: COMBINE_LIFTOFF_ANNOTATIONS { + ext.args = '-T' + } + + withName: GFFREAD_AFTER_LIFTOFF { + ext.args = '--keep-genes --keep-comments --keep-exon-attrs -F' + + publishDir = [ + path: { "${params.outdir}/liftoff" }, + mode: "copy", + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + ] + } } } diff --git a/modules.json b/modules.json index adcf4de..49bab25 100644 --- a/modules.json +++ b/modules.json @@ -96,6 +96,11 @@ "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["fastq_fastqc_umitools_fastp", "modules"] }, + "gffcompare": { + "branch": "master", + "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "installed_by": ["modules"] + }, "gffread": { "branch": "master", "git_sha": "b1b959609bda44341120aed1766329909f54b8d0", diff --git a/modules/nf-core/gffcompare/environment.yml b/modules/nf-core/gffcompare/environment.yml new file mode 100644 index 0000000..bcd633e --- /dev/null +++ b/modules/nf-core/gffcompare/environment.yml @@ -0,0 +1,7 @@ +name: gffcompare +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::gffcompare=0.12.6 diff --git a/modules/nf-core/gffcompare/main.nf b/modules/nf-core/gffcompare/main.nf new file mode 100644 index 0000000..edca0f2 --- /dev/null +++ b/modules/nf-core/gffcompare/main.nf @@ -0,0 +1,63 @@ +process GFFCOMPARE { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/gffcompare:0.12.6--h9f5acd7_0' : + 'biocontainers/gffcompare:0.12.6--h9f5acd7_0' }" + + input: + tuple val(meta), path(gtfs) + tuple val(meta2), path(fasta), path(fai) + tuple val(meta3), path(reference_gtf) + + output: + tuple val(meta), path("*.annotated.gtf"), optional: true, emit: annotated_gtf + tuple val(meta), path("*.combined.gtf") , optional: true, emit: combined_gtf + tuple val(meta), path("*.tmap") , optional: true, emit: tmap + tuple val(meta), path("*.refmap") , optional: true, emit: refmap + tuple val(meta), path("*.loci") , emit: loci + tuple val(meta), path("*.stats") , emit: stats + tuple val(meta), path("*.tracking") , emit: tracking + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def ref_fasta = fasta ? "-s ${fasta}" : '' + def ref_gtf = reference_gtf ? "-r ${reference_gtf}" : '' + """ + gffcompare \\ + $args \\ + $ref_fasta \\ + $ref_gtf \\ + -o $prefix \\ + $gtfs + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gffcompare: \$(echo \$(gffcompare --version 2>&1) | sed 's/^gffcompare v//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.annotated.gtf + touch ${prefix}.combined.gtf + touch ${prefix}.tmap + touch ${prefix}.refmap + touch ${prefix}.loci + touch ${prefix}.stats + touch ${prefix}.tracking + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gffcompare: \$(echo \$(gffcompare --version 2>&1) | sed 's/^gffcompare v//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/gffcompare/meta.yml b/modules/nf-core/gffcompare/meta.yml new file mode 100644 index 0000000..674f08c --- /dev/null +++ b/modules/nf-core/gffcompare/meta.yml @@ -0,0 +1,91 @@ +name: "gffcompare" +description: Compare, merge, annotate and estimate accuracy of generated gtf files +keywords: + - transcripts + - gtf + - merge + - compare +tools: + - "gffcompare": + description: "GffCompare by Geo Pertea" + homepage: "http://ccb.jhu.edu/software/stringtie/gffcompare.shtml" + documentation: "http://ccb.jhu.edu/software/stringtie/gffcompare.shtml" + tool_dev_url: "https://github.com/gpertea/gffcompare" + doi: "10.12688/f1000research.23297.1" + licence: ["MIT"] +input: + - meta: + type: map + description: | + Groovy Map containing meta data + e.g. [ id:'test', single_end:false ] + - gtfs: + type: file + description: | + GTF/GFF files + e.g. [ 'file_1.gtf', 'file_2.gtf' ] + pattern: "*.{gtf,gff}" + - fasta: + type: file + description: Genome reference fasta file (optional) + pattern: "*.{fasta,fa}" + - fai: + type: file + description: Index for fasta file + pattern: "*.fai" + - reference_gtf: + type: file + description: Reference annotation in gtf/gff format (optional) + pattern: "*.{gtf,gff}" +output: + - meta: + type: map + description: | + Groovy Map containing meta data + e.g. [ id:'test', single_end:false ] + - annotated_gtf: + type: file + description: | + Annotated gtf file when reference gtf is provided (optional) + pattern: "*.annotated.gtf" + - combined_gtf: + type: file + description: | + Combined gtf file when multiple input files are + provided (optional) + pattern: "*.annotated.gtf" + - tmap: + type: file + description: | + File listing the most closely matching reference transcript + for each query transcript (optional) + pattern: "*.tmap" + - refmap: + type: file + description: | + File listing the reference transcripts with overlapping + query transcripts (optional) + pattern: "*.refmap" + - loci: + type: file + description: File with loci + pattern: "*.loci" + - stats: + type: file + description: | + File with stats for input transcripts as compared to + reference alternatively stats for the combined gtf + pattern: "*.stats" + - tracking: + type: file + description: | + This file matches transcripts up between samples + pattern: "*.tracking" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@jemten" +maintainers: + - "@jemten" diff --git a/subworkflows/local/fasta_liftoff.nf b/subworkflows/local/fasta_liftoff.nf index a842944..17dcf8b 100644 --- a/subworkflows/local/fasta_liftoff.nf +++ b/subworkflows/local/fasta_liftoff.nf @@ -1,7 +1,9 @@ -include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/gunzip' -include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/gunzip' -include { GFFREAD } from '../../modules/nf-core/gffread' -include { LIFTOFF } from '../../modules/nf-core/liftoff' +include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/gunzip/main' +include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/gunzip/main' +include { GFFREAD as GFFREAD_BEFORE_LIFTOFF } from '../../modules/nf-core/gffread/main' +include { LIFTOFF } from '../../modules/nf-core/liftoff/main' +include { GFFCOMPARE as COMBINE_LIFTOFF_ANNOTATIONS } from '../../modules/nf-core/gffcompare/main' +include { GFFREAD as GFFREAD_AFTER_LIFTOFF } from '../../modules/nf-core/gffread/main' workflow FASTA_LIFTOFF { take: @@ -44,21 +46,21 @@ workflow FASTA_LIFTOFF { ch_versions = ch_versions.mix(GUNZIP_GFF.out.versions.first()) - // MODULE: GFFREAD + // MODULE: GFFREAD as GFFREAD_BEFORE_LIFTOFF ch_gffread_inputs = ch_xref_gunzip_gff | map { meta, gff -> [ gff.baseName, meta, gff ] } // For meta insertion later, remove when GFFREAD has meta - GFFREAD ( ch_gffread_inputs.map { name, meta, gff -> gff } ) + GFFREAD_BEFORE_LIFTOFF ( ch_gffread_inputs.map { name, meta, gff -> gff } ) - ch_gffread_gff = GFFREAD.out.gffread_gff + ch_gffread_gff = GFFREAD_BEFORE_LIFTOFF.out.gffread_gff | map { gff -> [ gff.baseName - '.gffread', gff ] } | join(ch_gffread_inputs) | map { fid, gffread_gff, meta, gff -> [ meta, gffread_gff ] } // meta insertion - ch_versions = ch_versions.mix(GFFREAD.out.versions.first()) + ch_versions = ch_versions.mix(GFFREAD_BEFORE_LIFTOFF.out.versions.first()) // MODULE: LIFTOFF ch_liftoff_inputs = target_assemby @@ -93,7 +95,40 @@ workflow FASTA_LIFTOFF { ch_versions = ch_versions.mix(LIFTOFF.out.versions.first()) + // MODULE: GFFCOMPARE as COMBINE_LIFTOFF_ANNOTATIONS + ch_gffcompare_inputs = ch_liftoff_gff3 + | branch { meta, list_polished -> + one: list_polished.size() == 1 + many: list_polished.size() > 1 + } + + COMBINE_LIFTOFF_ANNOTATIONS( + ch_gffcompare_inputs.many, + [ [], [], [] ], + [ [], [] ], + ) + + ch_combined_gtf = COMBINE_LIFTOFF_ANNOTATIONS.out.combined_gtf + ch_versions = ch_versions.mix(COMBINE_LIFTOFF_ANNOTATIONS.out.versions.first()) + + // MODULE: GFFREAD as GFFREAD_AFTER_LIFTOFF + ch_post_gffread_inputs = ch_combined_gtf + | map { meta, gtf -> + [ gtf.baseName, meta, gtf ] + } // For meta insertion later, remove when GFFREAD has meta + + GFFREAD_AFTER_LIFTOFF ( ch_post_gffread_inputs.map { name, meta, gtf -> gtf } ) + + ch_combined_gff3 = GFFREAD_AFTER_LIFTOFF.out.gffread_gff + | map { gff -> [ gff.baseName - '.gffread', gff ] } + | join(ch_post_gffread_inputs) + | map { fid, gffread_gff, meta, gtf -> [ meta, gffread_gff ] } + // meta insertion + | mix(ch_gffcompare_inputs.one) + + ch_versions = ch_versions.mix(GFFREAD_AFTER_LIFTOFF.out.versions.first()) + emit: - gff3 = ch_liftoff_gff3 // [ meta, [ gff3 ] ] + gff3 = ch_combined_gff3 // [ meta, gff3 ] versions = ch_versions // [ versions.yml ] } From fb15149a4601073e5c9f9d925c8bf77fed55a9fa Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 25 Mar 2024 19:04:21 +1300 Subject: [PATCH 09/40] Renamed samplesheet param to fastq --- CHANGELOG.md | 1 + README.md | 14 +- bin/make-samplesheet.py | 285 ------------------ conf/test_params.json | 2 +- conf/test_stub.config | 2 +- .../main.nf | 12 +- .../resources/usr/bin/check_fastqsheet.py} | 0 nextflow.config | 5 +- subworkflows/local/extract_samples.nf | 16 +- subworkflows/local/preprocess_rnaseq.nf | 4 +- workflows/pangene.nf | 6 +- 11 files changed, 30 insertions(+), 317 deletions(-) delete mode 100755 bin/make-samplesheet.py rename modules/local/{samplesheet_check => fastqsheet_check}/main.nf (84%) rename modules/local/{samplesheet_check/resources/usr/bin/check_samplesheet.py => fastqsheet_check/resources/usr/bin/check_fastqsheet.py} (100%) diff --git a/CHANGELOG.md b/CHANGELOG.md index 12fefcc..222bcbf 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -21,6 +21,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 12. Now using empty files for stub testing in CI 13. Now BRAKER can be skipped by including BRAKER outputs from previous runs in the `target_assemblies` param 14. Added `gffcompare` to merge liftoff annotations +15. Renamed `samplesheet` param to `fastq` ### `Fixed` diff --git a/README.md b/README.md index 0717c20..2016044 100644 --- a/README.md +++ b/README.md @@ -30,8 +30,8 @@ flowchart TD TE_LIBRARIES --> REPEATMASKER te_lib_absent_node --> REPEATMASKER - subgraph Samplesheet [ ] - SAMPLESHEET + subgraph Fastq [ ] + FASTQ CAT_FASTQ FASTQC FASTP @@ -42,10 +42,10 @@ flowchart TD SAMTOOLS_CAT end - SAMPLESHEET([samplesheet]) - SAMPLESHEET --> |Tech. reps|CAT_FASTQ + FASTQ([fastq]) + FASTQ --> |Tech. reps|CAT_FASTQ CAT_FASTQ --> FASTQC - SAMPLESHEET --> FASTQC + FASTQ --> FASTQC FASTQC --> FASTP FASTP --> FASTP_FASTQC[FASTQC] FASTP_FASTQC --> SORTMERNA @@ -67,7 +67,7 @@ flowchart TD end PrepareAssembly --> |Fasta, Masked fasta|Annotation - Samplesheet --> |RNASeq bam|Annotation + Fastq --> |RNASeq bam|Annotation XREF_ANNOTATIONS --> |xref_gff|GFFREAD XREF_ANNOTATIONS --> |xref_fasta|LIFTOFF @@ -79,7 +79,7 @@ flowchart TD anno_bam --> |RNASeq bam|BRAKER3 CAT --> BRAKER3 - style Samplesheet fill:#00FFFF21,stroke:#00FFFF21 + style Fastq fill:#00FFFF21,stroke:#00FFFF21 style PrepareAssembly fill:#00FFFF21,stroke:#00FFFF21 style Annotation fill:#00FFFF21,stroke:#00FFFF21 ``` diff --git a/bin/make-samplesheet.py b/bin/make-samplesheet.py deleted file mode 100755 index b4ad0b7..0000000 --- a/bin/make-samplesheet.py +++ /dev/null @@ -1,285 +0,0 @@ -#!/usr/bin/env python3 - -import argparse -import pathlib -import os -import re -import pandas as pd -import subprocess -import hashlib - - -def remove_dna_symbols(string): - pattern = r"[ACGT]{5,}" - result = re.sub(pattern, "", string) - return result - - -def remove_lane_suffix(string): - pattern = r"_L0*\d*" - result = re.sub(pattern, "", string) - return result - - -def sort_list_of_files(files_list): - def natural_key(string): - return [int(s) if s.isdigit() else s for s in re.split(r"(\d+)", string)] - - return sorted(files_list, key=lambda x: natural_key(x)) - - -def validate_fastq_columns(fastq_1, fastq_2): - pattern = re.compile(r"(.*)([R]?[12])\.([fastq]+)\.gz$") - - for fq1, fq2 in zip(fastq_1, fastq_2): - match1 = pattern.match(fq1) - match2 = pattern.match(fq2) - - if match1 and match2: - base1 = match1.group(1) - base2 = match2.group(1) - if base1 != base2: - raise ValueError(f"Failed to match fq1 and fq2 files:\n{fq1}\n{fq2}") - else: - raise ValueError(f"Failed to match fq1 and fq2 files:\n{fq1}\n{fq2}") - - -def validate_samplesheet(pd_df): - if len(pd.unique(pd_df["sample"])) != len(pd_df["sample"]): - raise ValueError(f"Failed to create a sample-sheet with unique sample IDs") - - validate_fastq_columns(list(pd_df["fastq_1"]), list(pd_df["fastq_2"])) - - -def compute_md5(file_path): - with open(file_path, "rb") as file: - data = file.read(10_000_000) - md5_hash = hashlib.md5(data).hexdigest() - - print(f"Sample: {os.path.basename(file_path)}") - - return md5_hash - - -def remove_duplicate_samples(pd_df): - print("Checking for duplicate samples and removing them...") - - pd_df["md5sum"] = pd_df.apply( - lambda row: compute_md5(row["fastq_1"]) + compute_md5(row["fastq_2"]), axis=1 - ) - - duplicates = pd_df.duplicated(subset="md5sum") - - if duplicates.any(): - print("Following samples have duplicates:") - print(pd_df[duplicates].iloc[:, 0]) - else: - print("No duplicates detected...") - - df_unique = pd_df.drop_duplicates(subset="md5sum", keep="first") - return df_unique.iloc[:, :4] - - -def extract_r1_r2_files(list_of_files): - list_of_files_sorted = sort_list_of_files(list_of_files) - list_of_files_R1 = [ - f - for f in list_of_files_sorted - if len(re.findall(r"_[R]?1\.([fastq]+)\.gz", str(f))) > 0 - ] - list_of_files_R2 = [ - f - for f in list_of_files_sorted - if len(re.findall(r"_[R]?2\.([fastq]+)\.gz", str(f))) > 0 - ] - - if len(list_of_files_R1) != len(list_of_files_R2): - raise ValueError("Number of R1 and R2 files do not match") - - return list_of_files_R1, list_of_files_R2 - - -def get_common_literals(list_of_lists): - if len(list_of_lists) == 0: - return [] - - common_elements = set(list_of_lists[0]) - - for sublist in list_of_lists[1:]: - common_elements = common_elements.intersection(sublist) - - common_elements = list(common_elements) - - return sorted(common_elements) - - -def get_unique_elements(input_list): - unique_elements = [] - seen_elements = set() - for item in input_list: - if item not in seen_elements: - unique_elements.append(item) - seen_elements.add(item) - return unique_elements - - -def create_sample_ids_from_files_list(list_of_files_R1): - file_names_normalized = [ - f.replace(".fastq.gz", "") - .replace(".fq.gz", "") - .replace("-", "_") - .replace("/", "_") - .replace("R1", "") - for f in list_of_files_R1 - ] - - file_name_literals = [ - [l for l in f.split("_") if l != ""] for f in file_names_normalized - ] - - common_literals = get_common_literals(file_name_literals) - - cleaved_names = [] - for f in file_names_normalized: - cleaved_name = f - for l in common_literals: - cleaved_name = cleaved_name.replace(f"_{l}_", "__") - - cleaved_name = remove_dna_symbols(cleaved_name) - - cleaved_name_literals = [ - e for e in get_unique_elements(cleaved_name.split("_")) if e != "" - ] - - cleaved_name = "" - is_first = True - for em in cleaved_name_literals: - cleaved_name += em if is_first else "_" + em - is_first = False - - cleaved_names.append(cleaved_name) - - sample_ids = cleaved_names - - return sample_ids - - -def save_samplesheet(exp_name, list_of_files_R1, list_of_files_R2, sample_ids): - strandedness = ["auto" for _ in sample_ids] - file_data = pd.DataFrame( - { - "sample": sample_ids, - "fastq_1": list_of_files_R1, - "fastq_2": list_of_files_R2, - "strandedness": strandedness, - } - ) - - validate_samplesheet(file_data) - file_data.sort_values(by=["sample"], inplace=True) - - file_data_dedup = remove_duplicate_samples(file_data) - - file_data_dedup["sample"] = file_data_dedup["sample"].apply(remove_lane_suffix) - - file_data_dedup.to_csv(f"{exp_name}_samplesheet.csv", index=False) - - -def make_samplesheet_from_metadata_file(file_path, exp_name): - file_data = pd.read_excel(file_path, sheet_name="Samplesheet") - - sample_id_col = "isolate" - - file_data.loc[:, "sample"] = file_data[sample_id_col] - file_data.loc[:, "fastq_1"] = ( - file_data["directory"] + "/" + file_data["file_name_F"] - ) - file_data.loc[:, "fastq_2"] = ( - file_data["directory"] + "/" + file_data["file_name_R"] - ) - file_data.loc[:, "strandedness"] = "auto" - - file_data = file_data[["sample", "fastq_1", "fastq_2", "strandedness"]] - - validate_samplesheet(file_data) - file_data.sort_values(by=["sample"], inplace=True) - - file_data_dedup = remove_duplicate_samples(file_data) - file_data_dedup.to_csv(f"{exp_name}_samplesheet.csv", index=False) - - -def make_samplesheet_from_folder(file_path, exp_name): - if os.path.isfile(file_path): - raise ValueError( - "The provided path is for a file. Path to an input folder is required" - ) - - fastq_gz_list = [str(f) for f in file_path.glob("*.fastq.gz")] - fq_gz_list = [str(f) for f in file_path.glob("*.fq.gz")] - - list_of_files = fastq_gz_list + fq_gz_list - - if len(list_of_files) < 1: - raise ValueError( - "Could not find any fastq.gz or fq.gz files in the command output" - ) - - list_of_files_R1, list_of_files_R2 = extract_r1_r2_files(list_of_files) - sample_ids = create_sample_ids_from_files_list(list_of_files_R1) - save_samplesheet(exp_name, list_of_files_R1, list_of_files_R2, sample_ids) - - -def make_samplesheet_from_command(input_path_or_command, exp_name): - result = subprocess.run( - input_path_or_command, shell=True, capture_output=True, text=True - ) - - if result.returncode != 0: - raise ValueError(f"Failed to execute the provided command...\n{result.stderr}") - - list_of_files = [ - f - for f in result.stdout.split("\n") - if f != "" and (f.endswith(".fq.gz") or f.endswith(".fastq.gz")) - ] - - if len(list_of_files) < 1: - raise ValueError( - "Could not find any fastq.gz or fq.gz files in the command output" - ) - - list_of_files_R1, list_of_files_R2 = extract_r1_r2_files(list_of_files) - sample_ids = create_sample_ids_from_files_list(list_of_files_R1) - save_samplesheet(exp_name, list_of_files_R1, list_of_files_R2, sample_ids) - -def main(): - parser = argparse.ArgumentParser( - prog="make-sample-sheet", - description="Read an RNASeq input folder or metadata file and create a sample-sheet.csv", - ) - parser.add_argument( - "path", - help="RNASeq input folder path or metadata file path or a bash command which lists all the fastq samples", - ) - parser.add_argument( - "experiment-name", - help="RNASeq experiment name", - ) - parser.add_argument("-v", action="version", version="%(prog)s v0.3") - - args = vars(parser.parse_args()) - - input_path_or_command = args["path"] - exp_name = args["experiment-name"] - - print("Creating sample sheet...") - - if os.path.isfile(input_path_or_command): - make_samplesheet_from_metadata_file(input_path_or_command, exp_name) - elif os.path.isdir(input_path_or_command): - make_samplesheet_from_folder(pathlib.Path(input_path_or_command), exp_name) - else: - make_samplesheet_from_command(input_path_or_command, exp_name) - -if __name__ == "__main__": - main() diff --git a/conf/test_params.json b/conf/test_params.json index 8466dfa..8395de5 100644 --- a/conf/test_params.json +++ b/conf/test_params.json @@ -17,7 +17,7 @@ ["red5_v2p1", "pangene-test/te_lib/red5_v2p1.fa.gz"], ["donghong", "pangene-test/te_lib/donghong.TElib.fa.gz"] ], - "samplesheet": "pangene-test/fq/fastqsheet.csv", + "fastq": "pangene-test/fq/fastqsheet.csv", "remove_ribo_rna": true, "ribo_database_manifest": "assets/rrna-db-test.txt", "external_protein_fastas": [ diff --git a/conf/test_stub.config b/conf/test_stub.config index d90f41e..ce66577 100644 --- a/conf/test_stub.config +++ b/conf/test_stub.config @@ -8,7 +8,7 @@ params { ["donghong", "tests/stub/te_lib/donghong.TElib.fa.gz"] ] - samplesheet = "tests/stub/fq/fastqsheet.csv" + fastq = "tests/stub/fq/fastqsheet.csv" remove_ribo_rna = true ribo_database_manifest = "assets/rrna-db-test.txt" diff --git a/modules/local/samplesheet_check/main.nf b/modules/local/fastqsheet_check/main.nf similarity index 84% rename from modules/local/samplesheet_check/main.nf rename to modules/local/fastqsheet_check/main.nf index 4fb60f8..01acfce 100644 --- a/modules/local/samplesheet_check/main.nf +++ b/modules/local/fastqsheet_check/main.nf @@ -5,8 +5,8 @@ // Changes: // Added channel permissible_target_assemblies -process SAMPLESHEET_CHECK { - tag "$samplesheet" +process FASTQSHEET_CHECK { + tag "$fastqsheet" label 'process_single' conda "conda-forge::python=3.9.5" @@ -15,7 +15,7 @@ process SAMPLESHEET_CHECK { 'biocontainers/python:3.9--1' }" input: - path samplesheet + path fastqsheet val permissible_target_assemblies output: @@ -27,10 +27,10 @@ process SAMPLESHEET_CHECK { script: """ - check_samplesheet.py \\ - $samplesheet \\ + check_fastqsheet.py \\ + $fastqsheet \\ "$permissible_target_assemblies" \\ - samplesheet.valid.csv + fastqsheet.valid.csv cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/local/samplesheet_check/resources/usr/bin/check_samplesheet.py b/modules/local/fastqsheet_check/resources/usr/bin/check_fastqsheet.py similarity index 100% rename from modules/local/samplesheet_check/resources/usr/bin/check_samplesheet.py rename to modules/local/fastqsheet_check/resources/usr/bin/check_fastqsheet.py diff --git a/nextflow.config b/nextflow.config index 409da80..3e06ede 100644 --- a/nextflow.config +++ b/nextflow.config @@ -22,14 +22,11 @@ params { repeat_annotator = 'repeatmodeler' // 'repeatmodeler' or 'edta' - save_annotated_te_lib = true - edta_is_sensitive = false - repeatmasker_save_outputs = true - samplesheet = "/workspace/pangene/test_data/samplesheet.csv" + fastq = "/workspace/pangene/test_data/fastqsheet.csv" // Optional: Set to null if not available skip_fastqc = false diff --git a/subworkflows/local/extract_samples.nf b/subworkflows/local/extract_samples.nf index 8ddb7ae..aba7c82 100644 --- a/subworkflows/local/extract_samples.nf +++ b/subworkflows/local/extract_samples.nf @@ -2,7 +2,7 @@ // https://github.com/nf-core/rnaseq // MIT: https://github.com/nf-core/rnaseq/blob/master/LICENSE // -// Check input samplesheet and get read channels +// Check input fastqsheet and get read channels // // Changes: // Added channel permissible_target_assemblies @@ -11,19 +11,19 @@ // Nowing emitting an extra channel 'assemblies' which indicates the // assemblies targeted by each read -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' +include { FASTQSHEET_CHECK } from '../../modules/local/fastqsheet_check' workflow EXTRACT_SAMPLES { take: - samplesheet // file: /path/to/samplesheet.csv + fastqsheet // file: /path/to/fastqsheet.csv permissible_target_assemblies // val: assembly_a,assembly_b exclude_assemblies // channel: val(assembly_x,assembly_y) main: - SAMPLESHEET_CHECK ( samplesheet, permissible_target_assemblies ) + FASTQSHEET_CHECK ( fastqsheet, permissible_target_assemblies ) .csv | splitCsv ( header:true, sep:',' ) - | combine ( samplesheet ) + | combine ( fastqsheet ) | map { row, sheet -> create_fastq_channel(row, sheet.getParent()) } @@ -54,7 +54,7 @@ workflow EXTRACT_SAMPLES { emit: reads // channel: [ val(meta), [ reads ] ] assemblies // channel: [ val(meta), val(assembly) ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] + versions = FASTQSHEET_CHECK.out.versions // channel: [ versions.yml ] } // Function to get list of [ meta, [ fastq_1, fastq_2 ] ] @@ -71,13 +71,13 @@ def create_fastq_channel(LinkedHashMap row, sheetPath) { // add path(s) of the fastq file(s) to the meta map def fastq_meta = [] if (!file(fq1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${fq1}" + exit 1, "ERROR: Please check input fastqsheet -> Read 1 FastQ file does not exist!\n${fq1}" } if (meta.single_end) { fastq_meta = [ meta, [ file(fq1) ] ] } else { if (!file(fq2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${fq2}" + exit 1, "ERROR: Please check input fastqsheet -> Read 2 FastQ file does not exist!\n${fq2}" } fastq_meta = [ meta, [ file(fq1), file(fq2) ] ] } diff --git a/subworkflows/local/preprocess_rnaseq.nf b/subworkflows/local/preprocess_rnaseq.nf index 8557c0d..2847318 100644 --- a/subworkflows/local/preprocess_rnaseq.nf +++ b/subworkflows/local/preprocess_rnaseq.nf @@ -6,7 +6,7 @@ include { FASTQ_FASTQC_UMITOOLS_FASTP } from '../../subworkflows/nf-core/fastq workflow PREPROCESS_RNASEQ { take: - samplesheet // path: csv + fastqsheet // path: csv permissible_assemblies // val: assembly_a,assembly_b exclude_assemblies // channel: val(assembly_x,assembly_y) skip_fastqc // val: true|false @@ -21,7 +21,7 @@ workflow PREPROCESS_RNASEQ { // SUBWORKFLOW: EXTRACT_SAMPLES EXTRACT_SAMPLES( - samplesheet, + fastqsheet, permissible_assemblies, exclude_assemblies ) diff --git a/workflows/pangene.nf b/workflows/pangene.nf index 98c6dc1..b07ae62 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -48,8 +48,8 @@ workflow PANGENE { [ [ id:tag ], file(fasta, checkIfExists: true) ] } - ch_samplesheet = params.samplesheet - ? Channel.fromPath(params.samplesheet, checkIfExists: true) + ch_fastqsheet = params.fastq + ? Channel.fromPath(params.fastq, checkIfExists: true) : Channel.empty() ch_tar_assm_str = Channel.of( @@ -103,7 +103,7 @@ workflow PANGENE { // SUBWORKFLOW: PREPROCESS_RNASEQ PREPROCESS_RNASEQ( - ch_samplesheet, + ch_fastqsheet, ch_tar_assm_str, ch_braker_ex_asm_str, params.skip_fastqc, From 9765060213f2339d85b5628449f0c21e1cd44d0a Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 26 Mar 2024 17:20:03 +1300 Subject: [PATCH 10/40] Now using assemblysheet for inputs --- CHANGELOG.md | 2 + TODO.md | 2 - assets/schema_input.json | 69 ++++++++++++++++++++++++++++++++ conf/base.config | 5 +++ conf/test_params.json | 19 +-------- conf/test_stub.config | 8 +--- modules/local/validate_params.nf | 69 -------------------------------- nextflow.config | 23 +++-------- nextflow_schema.json | 40 ++++++++++++++++++ tests/stub/assemblysheet.csv | 3 ++ workflows/pangene.nf | 60 ++++++++++++++++++--------- 11 files changed, 168 insertions(+), 132 deletions(-) create mode 100644 assets/schema_input.json create mode 100644 nextflow_schema.json create mode 100644 tests/stub/assemblysheet.csv diff --git a/CHANGELOG.md b/CHANGELOG.md index 222bcbf..e9b549d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -22,6 +22,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 13. Now BRAKER can be skipped by including BRAKER outputs from previous runs in the `target_assemblies` param 14. Added `gffcompare` to merge liftoff annotations 15. Renamed `samplesheet` param to `fastq` +16. Now using assemblysheet in combination with nf-validation for assembly input +17. Added nextflow_schema.json ### `Fixed` diff --git a/TODO.md b/TODO.md index 3742442..59f5177 100644 --- a/TODO.md +++ b/TODO.md @@ -12,8 +12,6 @@ > https://www.biorxiv.org/content/10.1101/096529v2.full.pdf -- [ ] Sort out EDTA testing - - Mib finder, eggnog, blastp against TAIR and uniprot (Wait) - entap to merge (Wait) - trinity and PASA + StringTie2 -> Evigene (Do) diff --git a/assets/schema_input.json b/assets/schema_input.json new file mode 100644 index 0000000..da31bc3 --- /dev/null +++ b/assets/schema_input.json @@ -0,0 +1,69 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/plantandfoodresearch/pangene/master/assets/schema_input.json", + "title": "plantandfoodresearch/pangene pipeline - params.input schema", + "description": "Schema for the file provided with params.input", + "type": "array", + "items": { + "type": "object", + "properties": { + "tag": { + "type": "string", + "pattern": "^\\w+$", + "errorMessage": "Assembly tags must be provided and can only contain alphanumeric characters including '_'", + "unique": true + }, + "fasta": { + "type": "string", + "pattern": "^\\S+\\.f(a|asta|as|sa|na)(\\.gz)?$", + "errorMessage": "FASTA file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na)' or '.f(a|asta|as|sa|na).gz'" + }, + "is_masked": { + "type": "string", + "pattern": "^(yes|no)$", + "errorMessage": "Masking information must be provided as 'yes' or 'no'" + }, + "te_lib": { + "anyOf": [ + { + "type": "string", + "pattern": "^\\S+\\.f(a|asta|as|sa|na)(\\.gz)?$" + }, + { + "type": "string", + "maxLength": 0 + } + ], + "errorMessage": "TE library FASTA file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na)' or '.f(a|asta|as|sa|na).gz'" + }, + "annotation_gff3": { + "anyOf": [ + { + "type": "string", + "pattern": "^\\S+\\.gff(3)?(\\.gz)?$" + }, + { + "type": "string", + "maxLength": 0 + } + ], + "errorMessage": "Annotation GFF3 file path cannot contain spaces and must have extension '.gff.gz', '.gff3.gz', '.gff' or '.gff3'", + "dependentRequired": ["annotation_hints"] + }, + "annotation_hints": { + "anyOf": [ + { + "type": "string", + "pattern": "^\\S+\\.gff(3)?(\\.gz)?$" + }, + { + "type": "string", + "maxLength": 0 + } + ], + "errorMessage": "Annotation hints GFF/GFF3 file path cannot contain spaces and must have extension '.gff.gz', '.gff3.gz', '.gff' or '.gff3'" + } + }, + "required": ["tag", "fasta", "is_masked"] + } +} diff --git a/conf/base.config b/conf/base.config index 467a95b..24528ac 100644 --- a/conf/base.config +++ b/conf/base.config @@ -107,3 +107,8 @@ def check_max(obj, type) { } } } + +params { + validationSkipDuplicateCheck = true + validationS3PathCheck = true +} diff --git a/conf/test_params.json b/conf/test_params.json index 8395de5..3237ad7 100644 --- a/conf/test_params.json +++ b/conf/test_params.json @@ -1,22 +1,5 @@ { - "target_assemblies": [ - [ - "red5_v2p1", - "pangene-test/target/red5_v2p1_chr1.fasta.gz", - "pangene-test/braker/red5_v2p1.gff3.gz", - "pangene-test/braker/red5_v2p1.hints.gff.gz" - ], - [ - "donghong", - "pangene-test/target/donghong.chr1.fsa.gz", - "pangene-test/braker/donghong.gff3.gz", - "pangene-test/braker/donghong.hints.gff.gz" - ] - ], - "te_libraries": [ - ["red5_v2p1", "pangene-test/te_lib/red5_v2p1.fa.gz"], - ["donghong", "pangene-test/te_lib/donghong.TElib.fa.gz"] - ], + "input": "pangene-test/assemblysheet.csv", "fastq": "pangene-test/fq/fastqsheet.csv", "remove_ribo_rna": true, "ribo_database_manifest": "assets/rrna-db-test.txt", diff --git a/conf/test_stub.config b/conf/test_stub.config index ce66577..a3be497 100644 --- a/conf/test_stub.config +++ b/conf/test_stub.config @@ -1,12 +1,6 @@ params { - target_assemblies = [ - ["red5_v2p1", "tests/stub/target/red5_v2p1_chr1.fasta.gz"], - ["donghong", "tests/stub/target/donghong.chr1.fsa.gz"] - ] - te_libraries = [ - ["donghong", "tests/stub/te_lib/donghong.TElib.fa.gz"] - ] + input = "tests/stub/assemblysheet.csv" fastq = "tests/stub/fq/fastqsheet.csv" diff --git a/modules/local/validate_params.nf b/modules/local/validate_params.nf index 59102ed..bf52e4b 100644 --- a/modules/local/validate_params.nf +++ b/modules/local/validate_params.nf @@ -1,5 +1,4 @@ def validateParams(params) { - validateFastaTags(params) if (!params['repeat_annotator']) { error "Error: repeat_annotator must be either 'repeatmodeler' or 'edta'" @@ -9,78 +8,10 @@ def validateParams(params) { error "Error: repeat_annotator must be either 'repeatmodeler' or 'edta'" } - validateTETags(params) - validateTEFastaCorrespondence(params) - validateRiboDBManifest(params) - validateLiftoffXrefs(params) } -def validateFastaTags(params) { - def listOfFastaTuples = params["target_assemblies"] - - if ( isNotListOfLists(listOfFastaTuples, [ 2, 4 ] ) ) { - error 'Error: target_assemblies must be a list of sublists, with each sublist containing 2 or 4 elements' - } - - def fastaTags = listOfFastaTuples.collect { it[0] } - - fastaTags.each { - if (!(it =~ /^\w+$/)) { - error "Error: $it is not a valid tag in target_assemblies" - } - } - - if (fastaTags.size() != (fastaTags as Set).size()) { - error "All the tags in target_assemblies should be unique" - } -} - -def validateTETags(params) { - - if(!params["te_libraries"]) { - return - } - - def listOfTETuples = params["te_libraries"] - - if (listOfTETuples.isEmpty()) { - return - } - - if (isNotListOfLists(listOfTETuples, 2)) { - error 'Error: te_libraries must be a list of sublists, with each sublist containing 2 elements' - } - - def teTags = listOfTETuples.collect { it[0] } - - teTags.each { - if (!(it =~ /^\w+$/)) { - error "Error: $it is not a valid tag in te_libraries" - } - } -} - -def validateTEFastaCorrespondence(params) { - - if(!params["te_libraries"]) { - return - } - - def listOfTETuples = params["te_libraries"] - def listOfFastaTuples = params["target_assemblies"] - - def fastaTags = listOfFastaTuples.collect { it[0] } - def teTags = listOfTETuples.collect { it[0] } - - teTags.each { - if(!fastaTags.contains(it)) { - error "Error: $it in te_libraries does not have a corresponding tag in target_assemblies" - } - } -} - def validateRiboDBManifest(params) { if (params.remove_ribo_rna) { file_ribo_db = file(params.ribo_database_manifest, checkIfExists: true) diff --git a/nextflow.config b/nextflow.config index 3e06ede..c670a96 100644 --- a/nextflow.config +++ b/nextflow.config @@ -1,24 +1,7 @@ includeConfig './conf/base.config' params { - target_assemblies = [ - ["red5_v2p1", "/workspace/pangene/test_data/red5_v2p1_chr1.fasta"], - ["donghong", "/workspace/pangene/test_data/donghong.chr1.fsa.gz"] - ] - // Pattern: [ [tag, fasta(.gz) ] ] - // Permissible tags: tag, tag_1, tag_tag2_3, tag_tag2_tag3; - // Any name with alphanumeric characters including "_". - // "." is not allowed in the tag name - - te_libraries = [ - ["donghong", "/workspace/pangene/test_data/donghong.TElib.fa.gz"] - ] - // Pattern: [ [tag, fasta(.gz) ] ] - // Optional Set to null if libraries are not available. - // - // Each TE library should have an associated (by tag) assembly in target_assemblies. - // Not all target_assemblies need to have an associated (by tag) TE library. - // When the TE lib is not available for a traget assembly, EDTA is used to create one. + input = "/workspace/pangene/test_data/assemblysheet.csv" repeat_annotator = 'repeatmodeler' // 'repeatmodeler' or 'edta' @@ -82,3 +65,7 @@ params { includeConfig './conf/manifest.config' includeConfig './conf/modules.config' includeConfig './conf/reporting_defaults.config' + +plugins { + id 'nf-validation@1.1.3' +} diff --git a/nextflow_schema.json b/nextflow_schema.json new file mode 100644 index 0000000..3c8cda7 --- /dev/null +++ b/nextflow_schema.json @@ -0,0 +1,40 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/plantandfoodresearch/pangene/master/nextflow_schema.json", + "title": "plantandfoodresearch/pangene pipeline parameters", + "description": "A NextFlow pipeline for pan-genome annotation", + "type": "object", + "defs": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "", + "required": ["input", "outdir"], + "properties": { + "input": { + "type": "string", + "format": "file-path", + "mimetype": "text/csv", + "schema": "assets/schema_input.json", + "pattern": "^\\S+\\.csv$", + "description": "FASTA and other associated files for input assemblies provided as a formatted CSV file", + "help_text": "Input assembly sheet in CSV format", + "fa_icon": "fas fa-file-csv" + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open", + "default": "./results" + } + } + } + }, + "allOf": [ + { + "$ref": "#/defs/input_output_options" + } + ] +} diff --git a/tests/stub/assemblysheet.csv b/tests/stub/assemblysheet.csv new file mode 100644 index 0000000..d1d9be7 --- /dev/null +++ b/tests/stub/assemblysheet.csv @@ -0,0 +1,3 @@ +tag,fasta,is_masked,te_lib +red5_v2p1,tests/stub/target/red5_v2p1_chr1.fasta.gz,no, +donghong,tests/stub/target/donghong.chr1.fsa.gz,no,tests/stub/te_lib/donghong.TElib.fa.gz diff --git a/workflows/pangene.nf b/workflows/pangene.nf index b07ae62..ec576a5 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -1,3 +1,4 @@ +include { fromSamplesheet } from 'plugin/nf-validation' include { validateParams } from '../modules/local/validate_params' include { id_from_file_name } from '../modules/local/validate_params' include { PREPARE_ASSEMBLY } from '../subworkflows/local/prepare_assembly' @@ -12,23 +13,56 @@ validateParams(params) workflow PANGENE { + // Versions channel ch_versions = Channel.empty() - ch_target_assembly = Channel.fromList(params.target_assemblies) + // Input channels + ch_input = Channel.fromSamplesheet('input') + + ch_target_assembly = ch_input | map { it -> - def tag = it[0] - def fasta = it[1] + def tag = it[0] + def fasta = it[1] [ [ id: tag ], file(fasta, checkIfExists: true) ] } - ch_braker_annotation = Channel.fromList(params.target_assemblies) + ch_tar_assm_str = ch_input + | map { it -> + def tag = it[0].strip() + + tag + } + | collect + | map { it -> + it.join(",") + } + + ch_masked = ch_input + | map { it -> + def tag = it[0] + def is_masked = it[2] + + [ [ id: tag ], is_masked == "yes" ] + } + + ch_te_library = ch_input + | map { it -> + def tag = it[0] + def te_fasta = it[3] + + if ( te_fasta ) { + [ [ id:tag ], file(te_fasta, checkIfExists: true) ] + } + } + + ch_braker_annotation = ch_input | map { it -> - if ( it.size() == 4 ) { - def tag = it[0] - def braker_gff3 = it[2] - def hints_gff = it[3] + def tag = it[0] + def braker_gff3 = it[4] + def hints_gff = it[5] + if ( braker_gff3 ) { [ [ id: tag ], file(braker_gff3, checkIfExists: true), @@ -43,20 +77,10 @@ workflow PANGENE { | map { it.join(",") } | ifEmpty( "" ) - ch_te_library = Channel.fromList(params.te_libraries) - | map { tag, fasta -> - [ [ id:tag ], file(fasta, checkIfExists: true) ] - } - ch_fastqsheet = params.fastq ? Channel.fromPath(params.fastq, checkIfExists: true) : Channel.empty() - ch_tar_assm_str = Channel.of( - params.target_assemblies - .collect { it -> it[0].strip() }.join(",") // it[0] = tag - ) - ch_ribo_db = params.remove_ribo_rna ? file(params.ribo_database_manifest, checkIfExists: true) : null From 12a66ea6c1e463b098e4364a32dfd7538aac0c8e Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 27 Mar 2024 15:53:08 +1300 Subject: [PATCH 11/40] Now using nf-validation to validate fastqsheet provided by params.fastq --- CHANGELOG.md | 3 +- assets/schema_fastq.json | 44 ++++ conf/test_params.json | 2 +- conf/test_stub.config | 2 +- modules/local/fastqsheet_check/main.nf | 40 ---- .../resources/usr/bin/check_fastqsheet.py | 215 ------------------ modules/local/validate_params.nf | 33 ++- nextflow_schema.json | 10 + subworkflows/local/extract_samples.nf | 86 ------- subworkflows/local/preprocess_rnaseq.nf | 51 ++--- tests/stub/fastqsheet.csv | 4 + tests/stub/fq/fastqsheet.csv | 4 - workflows/pangene.nf | 31 ++- 13 files changed, 137 insertions(+), 388 deletions(-) create mode 100644 assets/schema_fastq.json delete mode 100644 modules/local/fastqsheet_check/main.nf delete mode 100755 modules/local/fastqsheet_check/resources/usr/bin/check_fastqsheet.py delete mode 100644 subworkflows/local/extract_samples.nf create mode 100644 tests/stub/fastqsheet.csv delete mode 100644 tests/stub/fq/fastqsheet.csv diff --git a/CHANGELOG.md b/CHANGELOG.md index e9b549d..fc7ad50 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 0.2.0+dev - [25-Mar-2024] +## 0.2.0+dev - [27-Mar-2024] ### `Added` @@ -24,6 +24,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 15. Renamed `samplesheet` param to `fastq` 16. Now using assemblysheet in combination with nf-validation for assembly input 17. Added nextflow_schema.json +18. Now using nf-validation to validate fastqsheet provided by params.fastq ### `Fixed` diff --git a/assets/schema_fastq.json b/assets/schema_fastq.json new file mode 100644 index 0000000..0890ce3 --- /dev/null +++ b/assets/schema_fastq.json @@ -0,0 +1,44 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/plantandfoodresearch/pangene/master/assets/schema_fastq.json", + "title": "plantandfoodresearch/pangene pipeline - params.fastq schema", + "description": "Schema for the file provided with params.fastq", + "type": "array", + "items": { + "type": "object", + "properties": { + "sample": { + "type": "string", + "pattern": "^\\S+$", + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] + }, + "fastq_1": { + "type": "string", + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" + }, + "fastq_2": { + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", + "anyOf": [ + { + "type": "string", + "pattern": "^\\S+\\.f(ast)?q\\.gz$" + }, + { + "type": "string", + "maxLength": 0 + } + ], + "dependentRequired": ["fastq_1"] + }, + "target_assemblies": { + "type": "string", + "pattern": "^(\\w+;)*\\w+$", + "errorMessage": "One or more target assemblies must be specified by their tags from params.input sheet. Multiple tags should be separated by ';'", + "meta": ["target_assemblies"] + } + }, + "required": ["sample", "fastq_1", "target_assemblies"] + } +} diff --git a/conf/test_params.json b/conf/test_params.json index 3237ad7..441a171 100644 --- a/conf/test_params.json +++ b/conf/test_params.json @@ -1,6 +1,6 @@ { "input": "pangene-test/assemblysheet.csv", - "fastq": "pangene-test/fq/fastqsheet.csv", + "fastq": "pangene-test/fastqsheet.csv", "remove_ribo_rna": true, "ribo_database_manifest": "assets/rrna-db-test.txt", "external_protein_fastas": [ diff --git a/conf/test_stub.config b/conf/test_stub.config index a3be497..922d328 100644 --- a/conf/test_stub.config +++ b/conf/test_stub.config @@ -2,7 +2,7 @@ params { input = "tests/stub/assemblysheet.csv" - fastq = "tests/stub/fq/fastqsheet.csv" + fastq = "tests/stub/fastqsheet.csv" remove_ribo_rna = true ribo_database_manifest = "assets/rrna-db-test.txt" diff --git a/modules/local/fastqsheet_check/main.nf b/modules/local/fastqsheet_check/main.nf deleted file mode 100644 index 01acfce..0000000 --- a/modules/local/fastqsheet_check/main.nf +++ /dev/null @@ -1,40 +0,0 @@ -// Source: -// https://github.com/nf-core/rnaseq -// MIT: https://github.com/nf-core/rnaseq/blob/master/LICENSE -// -// Changes: -// Added channel permissible_target_assemblies - -process FASTQSHEET_CHECK { - tag "$fastqsheet" - label 'process_single' - - conda "conda-forge::python=3.9.5" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.9--1' : - 'biocontainers/python:3.9--1' }" - - input: - path fastqsheet - val permissible_target_assemblies - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - """ - check_fastqsheet.py \\ - $fastqsheet \\ - "$permissible_target_assemblies" \\ - fastqsheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/local/fastqsheet_check/resources/usr/bin/check_fastqsheet.py b/modules/local/fastqsheet_check/resources/usr/bin/check_fastqsheet.py deleted file mode 100755 index ac75f2b..0000000 --- a/modules/local/fastqsheet_check/resources/usr/bin/check_fastqsheet.py +++ /dev/null @@ -1,215 +0,0 @@ -#!/usr/bin/env python3 - -import os -import sys -import errno -import argparse - -# https://github.com/nf-core/rnaseq -# MIT: https://github.com/nf-core/rnaseq/blob/master/LICENSE -# -# Changes: -# -# 1. Formatted with black -# 2. Added checks for the fifth column: target_assemblies -# 3. Removed strandedness - - -def parse_args(args=None): - Description = ( - "Reformat nf-core/rnaseq style samplesheet file and check its contents." - ) - Epilog = 'Example usage: python check_samplesheet.py "target_assembly_a,target_assembly_b" ' - - parser = argparse.ArgumentParser(description=Description, epilog=Epilog) - parser.add_argument("FILE_IN", help="Input samplesheet file.") - parser.add_argument("TARGET_ASSEMBLIES", help="Permissible target assemblies") - parser.add_argument("FILE_OUT", help="Output file.") - return parser.parse_args(args) - - -def make_dir(path): - if len(path) > 0: - try: - os.makedirs(path) - except OSError as exception: - if exception.errno != errno.EEXIST: - raise exception - - -def print_error(error, context="Line", context_str=""): - error_str = f"ERROR: Please check samplesheet -> {error}" - if context != "" and context_str != "": - error_str = f"ERROR: Please check samplesheet -> {error}\n{context.strip()}: '{context_str.strip()}'" - print(error_str) - sys.exit(1) - - -def check_samplesheet(file_in, file_out, permissible_target_assemblies): - """ - This function checks that the samplesheet follows the following structure: - - sample,fastq_1,fastq_2,target_assemblies - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz,red5;red3 - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz,red5;red3 - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz,,red5 - """ - - sample_mapping_dict = {} - with open(file_in, "r", encoding="utf-8-sig") as fin: - ## Check header - MIN_COLS = 4 - HEADER = ["sample", "fastq_1", "fastq_2", "target_assemblies"] - header = [x.strip('"') for x in fin.readline().strip().split(",")] - if header[: len(HEADER)] != HEADER: - print( - f"ERROR: Please check samplesheet header -> {','.join(header)} != {','.join(HEADER)}" - ) - sys.exit(1) - - ## Check sample entries - for line in fin: - if line.strip(): - lspl = [x.strip().strip('"') for x in line.strip().split(",")] - - ## Check valid number of columns per row - if len(lspl) < len(HEADER): - print_error( - f"Invalid number of columns (minimum = {len(HEADER)})!", - "Line", - line, - ) - - num_cols = len([x for x in lspl[: len(HEADER)] if x]) - if num_cols < MIN_COLS: - print_error( - f"Invalid number of populated columns (minimum = {MIN_COLS})!", - "Line", - line, - ) - - ## Check sample name entries - sample, fastq_1, fastq_2, target_assemblies = lspl[ - : len(HEADER) - ] - - if sample.find(" ") != -1: - print( - f"WARNING: Spaces have been replaced by underscores for sample: {sample}" - ) - sample = sample.replace(" ", "_") - if not sample: - print_error("Sample entry has not been specified!", "Line", line) - - ## Check FastQ file extension - for fastq in [fastq_1, fastq_2]: - if fastq: - if fastq.find(" ") != -1: - print_error("FastQ file contains spaces!", "Line", line) - if not fastq.endswith(".fastq.gz") and not fastq.endswith( - ".fq.gz" - ): - print_error( - "FastQ file does not have extension '.fastq.gz' or '.fq.gz'!", - "Line", - line, - ) - - ## Auto-detect paired-end/single-end - sample_info = [] ## [single_end, fastq_1, fastq_2] - if sample and fastq_1 and fastq_2: ## Paired-end short reads - sample_info = ["0", fastq_1, fastq_2] - elif sample and fastq_1 and not fastq_2: ## Single-end short reads - sample_info = ["1", fastq_1, fastq_2] - else: - print_error( - "Invalid combination of columns provided!", "Line", line - ) - - ## Check if the target assemblies are permissible - target_assemblies_list = sorted( - [x.strip() for x in target_assemblies.strip().split(";")] - ) - - for assembly in target_assemblies_list: - if assembly in permissible_target_assemblies: - continue - - print_error( - f"Target assembly '{assembly}' is not one of {permissible_target_assemblies}!", - "Line", - line, - ) - - ## Create sample mapping dictionary = {sample: [[ single_end, fastq_1, fastq_2, strandedness, [target_assemblies] ]]} - sample_target_assemblies = ";".join(target_assemblies_list) - sample_info = ( - sample_info + lspl[len(HEADER) :] + [sample_target_assemblies] - ) - if sample not in sample_mapping_dict: - sample_mapping_dict[sample] = [sample_info] - else: - if sample_info in sample_mapping_dict[sample]: - print_error( - "Samplesheet contains duplicate rows!", "Line", line - ) - else: - sample_mapping_dict[sample].append(sample_info) - - ## Write validated samplesheet with appropriate columns - if len(sample_mapping_dict) > 0: - out_dir = os.path.dirname(file_out) - make_dir(out_dir) - with open(file_out, "w") as fout: - fout.write( - ",".join( - [ - "sample", - "single_end", - "fastq_1", - "fastq_2", - "target_assemblies", - ] - + header[len(HEADER) :] - ) - + "\n" - ) - for sample in sorted(sample_mapping_dict.keys()): - ## Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end - if not all( - x[0] == sample_mapping_dict[sample][0][0] - for x in sample_mapping_dict[sample] - ): - print_error( - f"Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end!", - "Sample", - sample, - ) - - ## Check that multiple runs of the same sample have same target assemblies - if not all( - x[3] == sample_mapping_dict[sample][0][3] - for x in sample_mapping_dict[sample] - ): - print_error( - f"Multiple runs of a sample must have the same target assemblies!", - "Sample", - sample, - ) - - for idx, val in enumerate(sample_mapping_dict[sample]): - fout.write(",".join([f"{sample}_T{idx+1}"] + val) + "\n") - else: - print_error(f"No entries to process!", "Samplesheet: {file_in}") - - -def main(args=None): - args = parse_args(args) - permissible_target_assemblies = [ - x.strip() for x in args.TARGET_ASSEMBLIES.strip().split(",") - ] - check_samplesheet(args.FILE_IN, args.FILE_OUT, permissible_target_assemblies) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/modules/local/validate_params.nf b/modules/local/validate_params.nf index bf52e4b..fcc5540 100644 --- a/modules/local/validate_params.nf +++ b/modules/local/validate_params.nf @@ -16,7 +16,7 @@ def validateRiboDBManifest(params) { if (params.remove_ribo_rna) { file_ribo_db = file(params.ribo_database_manifest, checkIfExists: true) - if (file_ribo_db.isEmpty()) {exit 1, "File provided with --ribo_database_manifest is empty: ${file_ribo_db.getName()}!"} + if (file_ribo_db.isEmpty()) { exit 1, "File provided with --ribo_database_manifest is empty: ${file_ribo_db.getName()}!" } } } @@ -39,9 +39,9 @@ def isNotListOfLists(thisOne, subListSize) { return (!(thisOne instanceof List) || thisOne.isEmpty() || thisOne.any { !( it instanceof List ) || !( subListSize.contains( it.size() ) ) }) } -def id_from_file_name(file_name) { +def idFromFileName(fileName) { - def trial = ( file_name + def trial = ( fileName ).replaceFirst( /\.f(ast)?q$/, '' ).replaceFirst( @@ -52,7 +52,30 @@ def id_from_file_name(file_name) { /\.gz$/, '' ) - if ( trial == file_name ) { return file_name } + if ( trial == fileName ) { return fileName } - return id_from_file_name ( trial ) + return idFromFileName ( trial ) +} + +def validateFastqMetadata(metas, fqs, permAssString) { + def permAssList = permAssString.split(",") + + // Check if each listed assembly is permissible + metas.each { meta -> + if ( meta.target_assemblies.any { !permAssList.contains( it ) } ) { + exit 1, "Sample ${meta.id} targets ${meta.target_assemblies} which are not in $permAssList" + } + } + + // Check if multiple runs of a sample have the same target assemblies + if ( metas.collect { meta -> meta.target_assemblies }.unique().size() > 1 ) { + error "Multiple runs of sample ${metas.first().id} must target same assemblies" + } + + // Check if multiple runs of a sample have the same endedness + if ( metas.collect { meta -> meta.single_end }.unique().size() > 1 ) { + error "Multiple runs of sample ${metas.first().id} must have same endedness" + } + + [ metas.first(), fqs ] } diff --git a/nextflow_schema.json b/nextflow_schema.json index 3c8cda7..f6e986d 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -22,6 +22,16 @@ "help_text": "Input assembly sheet in CSV format", "fa_icon": "fas fa-file-csv" }, + "fastq": { + "type": "string", + "format": "file-path", + "mimetype": "text/csv", + "schema": "assets/schema_fastq.json", + "pattern": "^\\S+\\.csv$", + "description": "FASTQ files for RNASeq samples corresponding to each target assembly provided as a formatted CSV file", + "help_text": "FASTQ sample sheet in CSV format", + "fa_icon": "fas fa-file-csv" + }, "outdir": { "type": "string", "format": "directory-path", diff --git a/subworkflows/local/extract_samples.nf b/subworkflows/local/extract_samples.nf deleted file mode 100644 index aba7c82..0000000 --- a/subworkflows/local/extract_samples.nf +++ /dev/null @@ -1,86 +0,0 @@ -// Source: -// https://github.com/nf-core/rnaseq -// MIT: https://github.com/nf-core/rnaseq/blob/master/LICENSE -// -// Check input fastqsheet and get read channels -// -// Changes: -// Added channel permissible_target_assemblies -// Changed file name from input_check.nf to extract_samples.nf -// Removed strandedness -// Nowing emitting an extra channel 'assemblies' which indicates the -// assemblies targeted by each read - -include { FASTQSHEET_CHECK } from '../../modules/local/fastqsheet_check' - -workflow EXTRACT_SAMPLES { - take: - fastqsheet // file: /path/to/fastqsheet.csv - permissible_target_assemblies // val: assembly_a,assembly_b - exclude_assemblies // channel: val(assembly_x,assembly_y) - - main: - FASTQSHEET_CHECK ( fastqsheet, permissible_target_assemblies ) - .csv - | splitCsv ( header:true, sep:',' ) - | combine ( fastqsheet ) - | map { row, sheet -> - create_fastq_channel(row, sheet.getParent()) - } - | set { ch_reads } - - reads = ch_reads - | combine( exclude_assemblies ) - | map { meta, fastq, ex_assemblies -> - def ex_list = ex_assemblies.split(",") - - if ( !( meta.target_assemblies.every { ex_list.contains( it ) } ) ) { - [ [ id:meta.id, single_end:meta.single_end ], fastq ] - } - } - - - assemblies = ch_reads - | combine( exclude_assemblies ) - | flatMap { meta, fastq, ex_assemblies -> - def ex_list = ex_assemblies.split(",") - - meta - .target_assemblies - .collect { assembly -> [ [ id:meta.id, single_end:meta.single_end ], assembly ] } - .findAll { _meta, assembly -> !( ex_list.contains( assembly ) ) } - } - - emit: - reads // channel: [ val(meta), [ reads ] ] - assemblies // channel: [ val(meta), val(assembly) ] - versions = FASTQSHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row, sheetPath) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - meta.target_assemblies = row.target_assemblies.split(";").sort() - - def fq1 = row.fastq_1.startsWith("/") ? row.fastq_1 : "$sheetPath/${row.fastq_1}" - def fq2 = row.fastq_2.startsWith("/") ? row.fastq_2 : "$sheetPath/${row.fastq_2}" - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(fq1).exists()) { - exit 1, "ERROR: Please check input fastqsheet -> Read 1 FastQ file does not exist!\n${fq1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(fq1) ] ] - } else { - if (!file(fq2).exists()) { - exit 1, "ERROR: Please check input fastqsheet -> Read 2 FastQ file does not exist!\n${fq2}" - } - fastq_meta = [ meta, [ file(fq1), file(fq2) ] ] - } - - return fastq_meta -} diff --git a/subworkflows/local/preprocess_rnaseq.nf b/subworkflows/local/preprocess_rnaseq.nf index 2847318..72fa176 100644 --- a/subworkflows/local/preprocess_rnaseq.nf +++ b/subworkflows/local/preprocess_rnaseq.nf @@ -1,12 +1,11 @@ include { CAT_FASTQ } from '../../modules/nf-core/cat/fastq' include { SORTMERNA as SORTMERNA_INDEX } from '../../modules/nf-core/sortmerna' include { SORTMERNA as SORTMERNA_READS } from '../../modules/nf-core/sortmerna' -include { EXTRACT_SAMPLES } from '../../subworkflows/local/extract_samples' include { FASTQ_FASTQC_UMITOOLS_FASTP } from '../../subworkflows/nf-core/fastq_fastqc_umitools_fastp' workflow PREPROCESS_RNASEQ { take: - fastqsheet // path: csv + ch_reads // channel: [ [ id, single_end, target_assemblies ], [ [ fq ] ] ] permissible_assemblies // val: assembly_a,assembly_b exclude_assemblies // channel: val(assembly_x,assembly_y) skip_fastqc // val: true|false @@ -19,35 +18,35 @@ workflow PREPROCESS_RNASEQ { main: ch_versions = Channel.empty() - // SUBWORKFLOW: EXTRACT_SAMPLES - EXTRACT_SAMPLES( - fastqsheet, - permissible_assemblies, - exclude_assemblies - ) + ch_fastq = ch_reads + | combine( exclude_assemblies ) + | map { meta, fqs, ex_assemblies -> + def ex_list = ex_assemblies.split(",") - ch_fastq = EXTRACT_SAMPLES.out.reads - | map { meta, fastq -> - groupID = meta.id - ~/_T\d+/ - [ meta + [id: groupID], fastq ] + if ( !( meta.target_assemblies.every { ex_list.contains( it ) } ) ) { + [ [ id:meta.id, single_end:meta.single_end ], fqs ] + } } - | groupTuple() - | branch { meta, fastq -> - single : fastq.size() == 1 - return [ meta, fastq.flatten() ] - multiple: fastq.size() > 1 - return [ meta, fastq.flatten() ] + | branch { meta, fqs -> + single : fqs.size() == 1 + return [ meta, fqs.flatten() ] + multiple: fqs.size() > 1 + return [ meta, fqs.flatten() ] } - ch_reads_target = EXTRACT_SAMPLES.out.assemblies - | map { meta, assembly -> - groupID = meta.id - ~/_T\d+/ - [ meta + [id: groupID], assembly ] + + ch_reads_target = ch_reads + | combine( exclude_assemblies ) + | flatMap { meta, fqs, ex_assemblies -> + def ex_list = ex_assemblies.split(",") + + meta + .target_assemblies + .collect { assembly -> [ [ id:meta.id, single_end:meta.single_end ], assembly ] } + .findAll { _meta, assembly -> !( ex_list.contains( assembly ) ) } } | unique - ch_versions = ch_versions.mix(EXTRACT_SAMPLES.out.versions) - // MODULES: CAT_FASTQ CAT_FASTQ ( ch_fastq.multiple ) @@ -118,7 +117,7 @@ workflow PREPROCESS_RNASEQ { emit: - trim_reads = ch_emitted_reads // channel: [ meta, [ fq ] ] - reads_target = ch_reads_target // channel: [ meta, assembly_id ] + trim_reads = ch_emitted_reads // channel: [ [ id, single_end ], [ fq ] ] + reads_target = ch_reads_target // channel: [ [ id, single_end ], assembly_id ] versions = ch_versions // channel: [ versions.yml ] } diff --git a/tests/stub/fastqsheet.csv b/tests/stub/fastqsheet.csv new file mode 100644 index 0000000..0b7e223 --- /dev/null +++ b/tests/stub/fastqsheet.csv @@ -0,0 +1,4 @@ +sample,fastq_1,fastq_2,target_assemblies +Root1,tests/stub/fq/1505KHS-0090_Root1_162bp_C728RACXX_Lane1_R1.1k.fastq.gz,tests/stub/fq/1505KHS-0090_Root1_162bp_C728RACXX_Lane1_R2.1k.fastq.gz,red5_v2p1 +Root1,tests/stub/fq/1505KHS-0090_Root2_156bp_C728RACXX_Lane1_R1.1k.fastq.gz,tests/stub/fq/1505KHS-0090_Root2_156bp_C728RACXX_Lane1_R2.1k.fastq.gz,red5_v2p1 +cane3,tests/stub/fq/1505KHS-0090_cane3_165bp_C728RACXX_Lane1_R1.1k.fastq.gz,tests/stub/fq/1505KHS-0090_cane3_165bp_C728RACXX_Lane1_R2.1k.fastq.gz,red5_v2p1;donghong diff --git a/tests/stub/fq/fastqsheet.csv b/tests/stub/fq/fastqsheet.csv deleted file mode 100644 index 7c35f1e..0000000 --- a/tests/stub/fq/fastqsheet.csv +++ /dev/null @@ -1,4 +0,0 @@ -sample,fastq_1,fastq_2,target_assemblies -Root1,1505KHS-0090_Root1_162bp_C728RACXX_Lane1_R1.1k.fastq.gz,1505KHS-0090_Root1_162bp_C728RACXX_Lane1_R2.1k.fastq.gz,red5_v2p1 -Root1,1505KHS-0090_Root2_156bp_C728RACXX_Lane1_R1.1k.fastq.gz,1505KHS-0090_Root2_156bp_C728RACXX_Lane1_R2.1k.fastq.gz,red5_v2p1 -cane3,1505KHS-0090_cane3_165bp_C728RACXX_Lane1_R1.1k.fastq.gz,1505KHS-0090_cane3_165bp_C728RACXX_Lane1_R2.1k.fastq.gz,red5_v2p1;donghong diff --git a/workflows/pangene.nf b/workflows/pangene.nf index ec576a5..5205957 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -1,6 +1,6 @@ include { fromSamplesheet } from 'plugin/nf-validation' include { validateParams } from '../modules/local/validate_params' -include { id_from_file_name } from '../modules/local/validate_params' +include { idFromFileName; validateFastqMetadata } from '../modules/local/validate_params' include { PREPARE_ASSEMBLY } from '../subworkflows/local/prepare_assembly' include { PREPROCESS_RNASEQ } from '../subworkflows/local/preprocess_rnaseq' include { ALIGN_RNASEQ } from '../subworkflows/local/align_rnaseq' @@ -77,9 +77,22 @@ workflow PANGENE { | map { it.join(",") } | ifEmpty( "" ) - ch_fastqsheet = params.fastq - ? Channel.fromPath(params.fastq, checkIfExists: true) - : Channel.empty() + ch_reads = ! params.fastq + ? Channel.empty() + : Channel.fromSamplesheet('fastq') + | map { meta, fq1, fq2 -> + fq2 + ? [ meta + [ single_end: false ], [ file(fq1, checkIfExists:true), file(fq2, checkIfExists:true) ] ] + : [ meta + [ single_end: true ], [ file(fq1, checkIfExists:true) ] ] + } + | map { meta, fqs -> + [ meta.id, meta + [ target_assemblies: meta.target_assemblies.split(';').sort() ], fqs ] + } + | groupTuple + | combine(ch_tar_assm_str) + | map { id, metas, fqs, tar_assm_str -> + validateFastqMetadata(metas, fqs, tar_assm_str) + } ch_ribo_db = params.remove_ribo_rna ? file(params.ribo_database_manifest, checkIfExists: true) @@ -94,8 +107,8 @@ workflow PANGENE { ch_ext_prot_fastas = params.external_protein_fastas ? Channel.fromList(params.external_protein_fastas) | map { filePath -> - def fileHandle = file(filePath, checkIfExists: true) - [ [ id: id_from_file_name( fileHandle.baseName ) ], fileHandle] + def file_handle = file(filePath, checkIfExists: true) + [ [ id: idFromFileName( file_handle.baseName ) ], file_handle] } : Channel.empty() @@ -104,8 +117,8 @@ workflow PANGENE { | multiMap { fasta, gff -> def fastaFile = file(fasta, checkIfExists:true) - fasta: [ [ id: id_from_file_name( fastaFile.baseName ) ], fastaFile ] - gff: [ [ id: id_from_file_name( fastaFile.baseName ) ], file(gff, checkIfExists:true) ] + fasta: [ [ id: idFromFileName( fastaFile.baseName ) ], fastaFile ] + gff: [ [ id: idFromFileName( fastaFile.baseName ) ], file(gff, checkIfExists:true) ] } : Channel.empty() @@ -127,7 +140,7 @@ workflow PANGENE { // SUBWORKFLOW: PREPROCESS_RNASEQ PREPROCESS_RNASEQ( - ch_fastqsheet, + ch_reads, ch_tar_assm_str, ch_braker_ex_asm_str, params.skip_fastqc, From df008f462186991ac864bf5a4b10633e0afb2c21 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 27 Mar 2024 16:02:58 +1300 Subject: [PATCH 12/40] Moved manifest.config and reporting_defaults.config content to nextflow.config --- CHANGELOG.md | 1 + conf/base.config | 11 ----------- conf/manifest.config | 10 ---------- conf/reporting_defaults.config | 13 ------------- nextflow.config | 34 +++++++++++++++++++++++++++++++--- version_check.sh | 2 +- 6 files changed, 33 insertions(+), 38 deletions(-) delete mode 100644 conf/manifest.config delete mode 100644 conf/reporting_defaults.config diff --git a/CHANGELOG.md b/CHANGELOG.md index fc7ad50..35d4818 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -25,6 +25,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 16. Now using assemblysheet in combination with nf-validation for assembly input 17. Added nextflow_schema.json 18. Now using nf-validation to validate fastqsheet provided by params.fastq +19. Moved `manifest.config` and `reporting_defaults.config` content to `nextflow.config` ### `Fixed` diff --git a/conf/base.config b/conf/base.config index 24528ac..3d30f22 100644 --- a/conf/base.config +++ b/conf/base.config @@ -71,12 +71,6 @@ process { } } -nextflow { - enable { - moduleBinaries = true - } -} - def check_max(obj, type) { if (type == 'memory') { try { @@ -107,8 +101,3 @@ def check_max(obj, type) { } } } - -params { - validationSkipDuplicateCheck = true - validationS3PathCheck = true -} diff --git a/conf/manifest.config b/conf/manifest.config deleted file mode 100644 index 93780a1..0000000 --- a/conf/manifest.config +++ /dev/null @@ -1,10 +0,0 @@ -manifest { - name = 'pangene' - author = """Usman Rashid, Jason Shiller""" - homePage = 'https://github.com/PlantandFoodResearch/pan-gene' - description = """A NextFlow pipeline for pan-genome annotation""" - mainScript = 'main.nf' - nextflowVersion = '!>=23.04.4' - version = '0.2.0+dev' - doi = '' -} diff --git a/conf/reporting_defaults.config b/conf/reporting_defaults.config deleted file mode 100644 index 178522d..0000000 --- a/conf/reporting_defaults.config +++ /dev/null @@ -1,13 +0,0 @@ -def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') -timeline { - enabled = true - file = "${params.outdir}/pipeline_info/execution_timeline_${trace_timestamp}.html" -} -report { - enabled = true - file = "${params.outdir}/pipeline_info/execution_report_${trace_timestamp}.html" -} -trace { - enabled = true - file = "${params.outdir}/pipeline_info/execution_trace_${trace_timestamp}.txt" -} diff --git a/nextflow.config b/nextflow.config index c670a96..c0a3147 100644 --- a/nextflow.config +++ b/nextflow.config @@ -62,10 +62,38 @@ params { max_time = 1.days } -includeConfig './conf/manifest.config' -includeConfig './conf/modules.config' -includeConfig './conf/reporting_defaults.config' +params { + validationSkipDuplicateCheck= true + validationS3PathCheck = true +} + +manifest { + name = 'pangene' + author = """Usman Rashid, Jason Shiller""" + homePage = 'https://github.com/PlantandFoodResearch/pan-gene' + description = """A NextFlow pipeline for pan-genome annotation""" + mainScript = 'main.nf' + nextflowVersion = '!>=23.04.4' + version = '0.2.0+dev' + doi = '' +} + +def trace_timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') +timeline { + enabled = true + file = "${params.outdir}/pipeline_info/execution_timeline_${trace_timestamp}.html" +} +report { + enabled = true + file = "${params.outdir}/pipeline_info/execution_report_${trace_timestamp}.html" +} +trace { + enabled = true + file = "${params.outdir}/pipeline_info/execution_trace_${trace_timestamp}.txt" +} plugins { id 'nf-validation@1.1.3' } + +includeConfig './conf/modules.config' diff --git a/version_check.sh b/version_check.sh index 815c086..131d97c 100755 --- a/version_check.sh +++ b/version_check.sh @@ -1,6 +1,6 @@ #!/usr/bin/env bash -config_version=$(sed -n "s/.*version.*= '\(.*\)'.*/\1/p" conf/manifest.config) +config_version=$(sed -n "s/.*version.*= '\(.*\)'.*/\1/p" nextflow.config) # Check CHANGELOG version From 92b3d2fbb28560619c95efd09b42af9c36af31d4 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 27 Mar 2024 19:19:04 +1300 Subject: [PATCH 13/40] Now using a txt file for params.external_protein_fastas --- CHANGELOG.md | 1 + conf/test_params.json | 5 +---- conf/test_stub.config | 5 +---- nextflow.config | 6 +----- pfr/params.json | 6 ++++++ tests/stub/external-protein-fastas.txt | 2 ++ workflows/pangene.nf | 13 +++++++------ 7 files changed, 19 insertions(+), 19 deletions(-) create mode 100644 pfr/params.json create mode 100644 tests/stub/external-protein-fastas.txt diff --git a/CHANGELOG.md b/CHANGELOG.md index 35d4818..22b55b9 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -26,6 +26,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 17. Added nextflow_schema.json 18. Now using nf-validation to validate fastqsheet provided by params.fastq 19. Moved `manifest.config` and `reporting_defaults.config` content to `nextflow.config` +20. Now using a txt file for `params.external_protein_fastas` ### `Fixed` diff --git a/conf/test_params.json b/conf/test_params.json index 441a171..63bb2e3 100644 --- a/conf/test_params.json +++ b/conf/test_params.json @@ -3,10 +3,7 @@ "fastq": "pangene-test/fastqsheet.csv", "remove_ribo_rna": true, "ribo_database_manifest": "assets/rrna-db-test.txt", - "external_protein_fastas": [ - "pangene-test/ext_prot/RU01.20221115150135.chr1.pep.fasta.gz", - "pangene-test/ext_prot/RU01.20221115150135.chr2.pep.fasta.gz" - ], + "external_protein_fastas": "pangene-test/external-protein-fastas.txt", "braker_extra_args": "--rounds=1", "liftoff_xref_annotations": [ ["pangene-test/liftoff/Russell_V2a.chr1.fsa.gz", "pangene-test/liftoff/RU01.20221115150135.chr1.gff3.gz"], diff --git a/conf/test_stub.config b/conf/test_stub.config index 922d328..3048c52 100644 --- a/conf/test_stub.config +++ b/conf/test_stub.config @@ -7,10 +7,7 @@ params { remove_ribo_rna = true ribo_database_manifest = "assets/rrna-db-test.txt" - external_protein_fastas = [ - "tests/stub/ext_prot/RU01.20221115150135.chr1.pep.fasta.gz", - "tests/stub/ext_prot/RU01.20221115150135.chr2.pep.fasta.gz" - ] + external_protein_fastas = "pangene-test/external-protein-fastas.txt" liftoff_xref_annotations = [ ["tests/stub/liftoff/Russell_V2a.chr1.fsa.gz", "tests/stub/liftoff/RU01.20221115150135.chr1.gff3.gz"], diff --git a/nextflow.config b/nextflow.config index c0a3147..77ef6c0 100644 --- a/nextflow.config +++ b/nextflow.config @@ -31,11 +31,7 @@ params { // A single BAM is created for each assembly from all the RNAseq samples, if there // are more than one - external_protein_fastas = [ - "/workspace/ComparativeDataSources/OrthoDB11/Viridiplantae.fa.gz", - "/output/genomic/fairGenomes/Plant/Actinidia/chinensis/var_chinensis/male/2x/assembly_russell/v2.1/RU01.20221115150135.pep.fasta" - ] - // Optional: Set to null if not available + external_protein_fastas = null braker_extra_args = "" diff --git a/pfr/params.json b/pfr/params.json new file mode 100644 index 0000000..ac7ecbd --- /dev/null +++ b/pfr/params.json @@ -0,0 +1,6 @@ +{ + "external_protein_fastas": [ + "/workspace/ComparativeDataSources/OrthoDB11/Viridiplantae.fa.gz", + "/output/genomic/fairGenomes/Plant/Actinidia/chinensis/var_chinensis/male/2x/assembly_russell/v2.1/RU01.20221115150135.pep.fasta" + ] +} diff --git a/tests/stub/external-protein-fastas.txt b/tests/stub/external-protein-fastas.txt new file mode 100644 index 0000000..33b3484 --- /dev/null +++ b/tests/stub/external-protein-fastas.txt @@ -0,0 +1,2 @@ +pangene-test/ext_prot/RU01.20221115150135.chr1.pep.fasta.gz +pangene-test/ext_prot/RU01.20221115150135.chr2.pep.fasta.gz diff --git a/workflows/pangene.nf b/workflows/pangene.nf index 5205957..437ea6e 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -104,13 +104,14 @@ workflow PANGENE { | collect : Channel.empty() - ch_ext_prot_fastas = params.external_protein_fastas - ? Channel.fromList(params.external_protein_fastas) - | map { filePath -> - def file_handle = file(filePath, checkIfExists: true) - [ [ id: idFromFileName( file_handle.baseName ) ], file_handle] + ch_ext_prot_fastas = ! params.external_protein_fastas + ? Channel.empty() + : Channel.fromPath(params.external_protein_fastas) + | splitText + | map { file_path -> + def file_handle = file(file_path.strip(), checkIfExists: true) + [ [ id: idFromFileName( file_handle.baseName ) ], file_handle ] } - : Channel.empty() ch_xref_mm = params.liftoff_xref_annotations ? Channel.fromList(params.liftoff_xref_annotations) From 8ffcc32c8fc8a2c33129a18d5282faf49cc77d53 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 27 Mar 2024 20:06:15 +1300 Subject: [PATCH 14/40] Now using nf-validation for params.liftoff_annotations --- CHANGELOG.md | 1 + assets/schema_liftoff.json | 25 +++++++++++++++++++++++++ conf/modules.config | 2 +- conf/test_params.json | 5 +---- conf/test_stub.config | 5 +---- modules/local/validate_params.nf | 20 -------------------- nextflow.config | 13 +------------ nextflow_schema.json | 10 ++++++++++ pfr/params.json | 10 ++++++++++ tests/stub/liftoffannotations.csv | 3 +++ workflows/pangene.nf | 14 +++++++------- 11 files changed, 60 insertions(+), 48 deletions(-) create mode 100644 assets/schema_liftoff.json create mode 100644 tests/stub/liftoffannotations.csv diff --git a/CHANGELOG.md b/CHANGELOG.md index 22b55b9..310d882 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -27,6 +27,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 18. Now using nf-validation to validate fastqsheet provided by params.fastq 19. Moved `manifest.config` and `reporting_defaults.config` content to `nextflow.config` 20. Now using a txt file for `params.external_protein_fastas` +21. Now using nf-validation for `params.liftoff_annotations` ### `Fixed` diff --git a/assets/schema_liftoff.json b/assets/schema_liftoff.json new file mode 100644 index 0000000..f4bb651 --- /dev/null +++ b/assets/schema_liftoff.json @@ -0,0 +1,25 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/plantandfoodresearch/pangene/master/assets/schema_fastq.json", + "title": "plantandfoodresearch/pangene pipeline - params.fastq schema", + "description": "Schema for the file provided with params.fastq", + "type": "array", + "items": { + "type": "object", + "properties": { + "fasta": { + "type": "string", + "pattern": "^\\S+\\.f(a|asta|as|sa|na)(\\.gz)?$", + "errorMessage": "FASTA file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na)' or '.f(a|asta|as|sa|na).gz'", + "unique": true + }, + "gff3": { + "type": "string", + "pattern": "^\\S+\\.gff(3)?(\\.gz)?$", + "errorMessage": "Annotation GFF3 file path cannot contain spaces and must have extension '.gff.gz', '.gff3.gz', '.gff' or '.gff3'", + "unique": true + } + }, + "required": ["fasta", "gff3"] + } +} diff --git a/conf/modules.config b/conf/modules.config index b5dc000..557fc2a 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -154,7 +154,7 @@ process { } } -if(params.liftoff_xref_annotations) { +if(params.liftoff_annotations) { process { withName: LIFTOFF { ext.args = ' ' diff --git a/conf/test_params.json b/conf/test_params.json index 63bb2e3..e79596e 100644 --- a/conf/test_params.json +++ b/conf/test_params.json @@ -5,10 +5,7 @@ "ribo_database_manifest": "assets/rrna-db-test.txt", "external_protein_fastas": "pangene-test/external-protein-fastas.txt", "braker_extra_args": "--rounds=1", - "liftoff_xref_annotations": [ - ["pangene-test/liftoff/Russell_V2a.chr1.fsa.gz", "pangene-test/liftoff/RU01.20221115150135.chr1.gff3.gz"], - ["pangene-test/liftoff/Russell_V2a.chr2.fsa.gz", "pangene-test/liftoff/RU01.20221115150135.chr2.gff3.gz"] - ], + "liftoff_annotations": "pangene-test/liftoffannotations.csv", "max_cpus": 8, "max_memory": "32.GB" } diff --git a/conf/test_stub.config b/conf/test_stub.config index 3048c52..bd2006b 100644 --- a/conf/test_stub.config +++ b/conf/test_stub.config @@ -9,10 +9,7 @@ params { external_protein_fastas = "pangene-test/external-protein-fastas.txt" - liftoff_xref_annotations = [ - ["tests/stub/liftoff/Russell_V2a.chr1.fsa.gz", "tests/stub/liftoff/RU01.20221115150135.chr1.gff3.gz"], - ["tests/stub/liftoff/Russell_V2a.chr2.fsa.gz", "tests/stub/liftoff/RU01.20221115150135.chr2.gff3.gz"] - ] + liftoff_xref_annotations = "tests/stub/liftoffannotations.csv" max_cpus = 2 max_memory = 3.GB diff --git a/modules/local/validate_params.nf b/modules/local/validate_params.nf index fcc5540..6bdf0f7 100644 --- a/modules/local/validate_params.nf +++ b/modules/local/validate_params.nf @@ -9,7 +9,6 @@ def validateParams(params) { } validateRiboDBManifest(params) - validateLiftoffXrefs(params) } def validateRiboDBManifest(params) { @@ -20,25 +19,6 @@ def validateRiboDBManifest(params) { } } -def validateLiftoffXrefs(params) { - if(!params["liftoff_xref_annotations"]) { - return - } - - if(isNotListOfLists(params["liftoff_xref_annotations"]), 2) { - error "Error: liftoff_xref_annotations must be a list of sublists, with each sublist containing 2 elements" - } -} - -def isNotListOfLists(thisOne, subListSize) { - - if ( subListSize instanceof Integer ) { - return (!(thisOne instanceof List) || thisOne.isEmpty() || thisOne.any { !(it instanceof List) || it.size() != subListSize }) - } - - return (!(thisOne instanceof List) || thisOne.isEmpty() || thisOne.any { !( it instanceof List ) || !( subListSize.contains( it.size() ) ) }) -} - def idFromFileName(fileName) { def trial = ( fileName diff --git a/nextflow.config b/nextflow.config index 77ef6c0..5c61896 100644 --- a/nextflow.config +++ b/nextflow.config @@ -35,18 +35,7 @@ params { braker_extra_args = "" - liftoff_xref_annotations = [ - [ - "/output/genomic/fairGenomes/Plant/Actinidia/chinensis/var_chinensis/male/2x/assembly_russell/v2.1/Russell_V2a.chromosomes.and.unassiged.and.haplotigs.fsa", - "/output/genomic/fairGenomes/Plant/Actinidia/chinensis/var_chinensis/male/2x/assembly_russell/v2.1/RU01.20221115150135.gff3" - ], - [ - "/output/genomic/fairGenomes/Plant/Arabidopsis/thaliana/var_na/sex_na/2x/assembly_tair/v10/TAIR10_chr_all.fas", - "/output/genomic/fairGenomes/Plant/Arabidopsis/thaliana/var_na/sex_na/2x/assembly_tair/v10/TAIR10_GFF3_genes_transposons.fixed.gff3" - ] - ] - // Format: [ [ fasta(.gz), gff3(.gz) ] ] - // Optional: Set to null if not available + liftoff_annotations = null liftoff_coverage = 0.9 liftoff_identity = 0.9 diff --git a/nextflow_schema.json b/nextflow_schema.json index f6e986d..22a38de 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -32,6 +32,16 @@ "help_text": "FASTQ sample sheet in CSV format", "fa_icon": "fas fa-file-csv" }, + "liftoff_annotations": { + "type": "string", + "format": "file-path", + "mimetype": "text/csv", + "schema": "assets/schema_liftoff.json", + "pattern": "^\\S+\\.csv$", + "description": "FASTA and GFF3 files for annotations to use for liftoff", + "help_text": "Reference annotations to use for liftoff listed in CSV format", + "fa_icon": "fas fa-file-csv" + }, "outdir": { "type": "string", "format": "directory-path", diff --git a/pfr/params.json b/pfr/params.json index ac7ecbd..e8ca7e4 100644 --- a/pfr/params.json +++ b/pfr/params.json @@ -2,5 +2,15 @@ "external_protein_fastas": [ "/workspace/ComparativeDataSources/OrthoDB11/Viridiplantae.fa.gz", "/output/genomic/fairGenomes/Plant/Actinidia/chinensis/var_chinensis/male/2x/assembly_russell/v2.1/RU01.20221115150135.pep.fasta" + ], + "liftoff": [ + [ + "/output/genomic/fairGenomes/Plant/Actinidia/chinensis/var_chinensis/male/2x/assembly_russell/v2.1/Russell_V2a.chromosomes.and.unassiged.and.haplotigs.fsa", + "/output/genomic/fairGenomes/Plant/Actinidia/chinensis/var_chinensis/male/2x/assembly_russell/v2.1/RU01.20221115150135.gff3" + ], + [ + "/output/genomic/fairGenomes/Plant/Arabidopsis/thaliana/var_na/sex_na/2x/assembly_tair/v10/TAIR10_chr_all.fas", + "/output/genomic/fairGenomes/Plant/Arabidopsis/thaliana/var_na/sex_na/2x/assembly_tair/v10/TAIR10_GFF3_genes_transposons.fixed.gff3" + ] ] } diff --git a/tests/stub/liftoffannotations.csv b/tests/stub/liftoffannotations.csv new file mode 100644 index 0000000..8e1da9e --- /dev/null +++ b/tests/stub/liftoffannotations.csv @@ -0,0 +1,3 @@ +fasta,gff3 +pangene-test/liftoff/Russell_V2a.chr1.fsa.gz,pangene-test/liftoff/RU01.20221115150135.chr1.gff3.gz +pangene-test/liftoff/Russell_V2a.chr2.fsa.gz,pangene-test/liftoff/RU01.20221115150135.chr2.gff3.gz diff --git a/workflows/pangene.nf b/workflows/pangene.nf index 437ea6e..294dee3 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -113,18 +113,18 @@ workflow PANGENE { [ [ id: idFromFileName( file_handle.baseName ) ], file_handle ] } - ch_xref_mm = params.liftoff_xref_annotations - ? Channel.fromList(params.liftoff_xref_annotations) + ch_liftoff_mm = ! params.liftoff_annotations + ? Channel.empty() + : Channel.fromSamplesheet('liftoff_annotations') | multiMap { fasta, gff -> def fastaFile = file(fasta, checkIfExists:true) fasta: [ [ id: idFromFileName( fastaFile.baseName ) ], fastaFile ] gff: [ [ id: idFromFileName( fastaFile.baseName ) ], file(gff, checkIfExists:true) ] } - : Channel.empty() - ch_xref_fasta = ch_xref_mm.fasta - ch_xref_gff = ch_xref_mm.gff + ch_liftoff_fasta = ch_liftoff_mm.fasta + ch_liftoff_gff = ch_liftoff_mm.gff // SUBWORKFLOW: PREPARE_ASSEMBLY PREPARE_ASSEMBLY( @@ -190,8 +190,8 @@ workflow PANGENE { // SUBWORKFLOW: FASTA_LIFTOFF FASTA_LIFTOFF( ch_valid_target_assembly, - ch_xref_fasta, - ch_xref_gff + ch_liftoff_fasta, + ch_liftoff_gff ) ch_liftoff_gff3 = FASTA_LIFTOFF.out.gff3 From f028dcdfbbe6c4264f2f459ec400dbd16bb39bb9 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 28 Mar 2024 13:59:46 +1300 Subject: [PATCH 15/40] Now using nf-validation for all the parameters --- CHANGELOG.md | 1 + conf/test_params.json | 4 +- conf/test_stub.config | 8 +- main.nf | 6 +- .../local/{validate_params.nf => utils.nf} | 21 -- nextflow.config | 47 ++-- nextflow_schema.json | 226 +++++++++++++++++- workflows/pangene.nf | 7 +- 8 files changed, 246 insertions(+), 74 deletions(-) rename modules/local/{validate_params.nf => utils.nf} (66%) diff --git a/CHANGELOG.md b/CHANGELOG.md index 310d882..3af66af 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -28,6 +28,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 19. Moved `manifest.config` and `reporting_defaults.config` content to `nextflow.config` 20. Now using a txt file for `params.external_protein_fastas` 21. Now using nf-validation for `params.liftoff_annotations` +22. Now using nf-validation for all the parameters ### `Fixed` diff --git a/conf/test_params.json b/conf/test_params.json index e79596e..609fe6a 100644 --- a/conf/test_params.json +++ b/conf/test_params.json @@ -1,11 +1,11 @@ { "input": "pangene-test/assemblysheet.csv", "fastq": "pangene-test/fastqsheet.csv", + "external_protein_fastas": "pangene-test/external-protein-fastas.txt", + "liftoff_annotations": "pangene-test/liftoffannotations.csv", "remove_ribo_rna": true, "ribo_database_manifest": "assets/rrna-db-test.txt", - "external_protein_fastas": "pangene-test/external-protein-fastas.txt", "braker_extra_args": "--rounds=1", - "liftoff_annotations": "pangene-test/liftoffannotations.csv", "max_cpus": 8, "max_memory": "32.GB" } diff --git a/conf/test_stub.config b/conf/test_stub.config index bd2006b..2ebb70a 100644 --- a/conf/test_stub.config +++ b/conf/test_stub.config @@ -1,16 +1,10 @@ params { input = "tests/stub/assemblysheet.csv" - fastq = "tests/stub/fastqsheet.csv" - - remove_ribo_rna = true - ribo_database_manifest = "assets/rrna-db-test.txt" - external_protein_fastas = "pangene-test/external-protein-fastas.txt" - liftoff_xref_annotations = "tests/stub/liftoffannotations.csv" max_cpus = 2 - max_memory = 3.GB + max_memory = '3.GB' } diff --git a/main.nf b/main.nf index 9ed32f7..067425b 100755 --- a/main.nf +++ b/main.nf @@ -2,7 +2,11 @@ nextflow.enable.dsl=2 -include { PANGENE } from './workflows/pangene.nf' +include { validateParameters } from 'plugin/nf-validation' + +validateParameters() + +include { PANGENE } from './workflows/pangene.nf' workflow { PFR_PANGENE() diff --git a/modules/local/validate_params.nf b/modules/local/utils.nf similarity index 66% rename from modules/local/validate_params.nf rename to modules/local/utils.nf index 6bdf0f7..7e1c86a 100644 --- a/modules/local/validate_params.nf +++ b/modules/local/utils.nf @@ -1,24 +1,3 @@ -def validateParams(params) { - - if (!params['repeat_annotator']) { - error "Error: repeat_annotator must be either 'repeatmodeler' or 'edta'" - } - - if ( !(params['repeat_annotator'] in ['repeatmodeler', 'edta']) ) { - error "Error: repeat_annotator must be either 'repeatmodeler' or 'edta'" - } - - validateRiboDBManifest(params) -} - -def validateRiboDBManifest(params) { - if (params.remove_ribo_rna) { - file_ribo_db = file(params.ribo_database_manifest, checkIfExists: true) - - if (file_ribo_db.isEmpty()) { exit 1, "File provided with --ribo_database_manifest is empty: ${file_ribo_db.getName()}!" } - } -} - def idFromFileName(fileName) { def trial = ( fileName diff --git a/nextflow.config b/nextflow.config index 5c61896..5f388ff 100644 --- a/nextflow.config +++ b/nextflow.config @@ -1,53 +1,46 @@ includeConfig './conf/base.config' params { - input = "/workspace/pangene/test_data/assemblysheet.csv" + // Input/output options + input = null + fastq = null + external_protein_fastas = null + liftoff_annotations = null + outdir = "./results" + // Repeat annotation options repeat_annotator = 'repeatmodeler' - // 'repeatmodeler' or 'edta' - save_annotated_te_lib = true + save_annotated_te_lib = false edta_is_sensitive = false - repeatmasker_save_outputs = true - - fastq = "/workspace/pangene/test_data/fastqsheet.csv" - // Optional: Set to null if not available + repeatmasker_save_outputs = false + // RNASeq pre-processing options skip_fastqc = false skip_fastp = false min_trimmed_reads = 10000 extra_fastp_args = "" - - save_trimmed = true - // toggling this parameter results in rerun of FASTP and FASTQC_TRIM - + save_trimmed = false remove_ribo_rna = false - save_non_ribo_reads = true + save_non_ribo_reads = false ribo_database_manifest = "${projectDir}/assets/rrna-db-defaults.txt" + // RNAseq alignment options star_max_intron_length = 16000 star_align_extra_args = "" - star_save_outputs = true - save_cat_bam = true - // A single BAM is created for each assembly from all the RNAseq samples, if there - // are more than one - - external_protein_fastas = null + star_save_outputs = false + save_cat_bam = false + // Annotation options braker_extra_args = "" - - liftoff_annotations = null - liftoff_coverage = 0.9 liftoff_identity = 0.9 - outdir = "./results" - + // Max job request options max_cpus = 12 - max_memory = 200.GB - max_time = 1.days -} + max_memory = '200.GB' + max_time = '1.day' -params { + // Infrastructure options validationSkipDuplicateCheck= true validationS3PathCheck = true } diff --git a/nextflow_schema.json b/nextflow_schema.json index 22a38de..3b19410 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -4,7 +4,7 @@ "title": "plantandfoodresearch/pangene pipeline parameters", "description": "A NextFlow pipeline for pan-genome annotation", "type": "object", - "defs": { + "definitions": { "input_output_options": { "title": "Input/output options", "type": "object", @@ -18,9 +18,9 @@ "mimetype": "text/csv", "schema": "assets/schema_input.json", "pattern": "^\\S+\\.csv$", - "description": "FASTA and other associated files for input assemblies provided as a formatted CSV file", - "help_text": "Input assembly sheet in CSV format", - "fa_icon": "fas fa-file-csv" + "description": "Target assemblies listed in a CSV sheet", + "fa_icon": "fas fa-file-csv", + "help_text": "FASTA and other associated files for target assemblies provided as a CSV sheet" }, "fastq": { "type": "string", @@ -28,9 +28,17 @@ "mimetype": "text/csv", "schema": "assets/schema_fastq.json", "pattern": "^\\S+\\.csv$", - "description": "FASTQ files for RNASeq samples corresponding to each target assembly provided as a formatted CSV file", - "help_text": "FASTQ sample sheet in CSV format", - "fa_icon": "fas fa-file-csv" + "help_text": "FASTQ files for RNASeq samples corresponding to each target assembly provided in a CSV sheet", + "fa_icon": "fas fa-file-csv", + "description": "FASTQ samples listed in a CSV sheet" + }, + "external_protein_fastas": { + "type": "string", + "description": "External protein fastas listed in a text sheet", + "help_text": "A text file listing FASTA files to provide protein evidence for annotation", + "format": "file-path", + "mimetype": "text/txt", + "fa_icon": "far fa-file-alt" }, "liftoff_annotations": { "type": "string", @@ -38,23 +46,217 @@ "mimetype": "text/csv", "schema": "assets/schema_liftoff.json", "pattern": "^\\S+\\.csv$", - "description": "FASTA and GFF3 files for annotations to use for liftoff", - "help_text": "Reference annotations to use for liftoff listed in CSV format", + "description": "Reference annotations listed in a CSV sheet", + "help_text": "FASTA and GFF3 files for reference annotations for liftoff listed in a CSV sheet", "fa_icon": "fas fa-file-csv" }, "outdir": { "type": "string", "format": "directory-path", - "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "description": "The output directory where the results will be saved", "fa_icon": "fas fa-folder-open", - "default": "./results" + "default": "./results", + "help_text": " Use absolute paths to storage on Cloud infrastructure" + } + } + }, + "repeat_annotation_options": { + "title": "Repeat annotation options", + "type": "object", + "description": "", + "default": "", + "properties": { + "repeat_annotator": { + "type": "string", + "default": "repeatmodeler", + "enum": ["edta", "repeatmodeler"], + "description": "'edta' or 'repeatmodeler'" + }, + "save_annotated_te_lib": { + "type": "boolean", + "description": "Save annotated TE library or not?" + }, + "edta_is_sensitive": { + "type": "boolean", + "description": "Use '--sensitive 1' flag with EDTA or not?" + }, + "repeatmasker_save_outputs": { + "type": "boolean", + "description": "Save the repeat-masked genome or not?" + } + } + }, + "rnaseq_pre_processing_options": { + "title": "RNASeq pre-processing options", + "type": "object", + "description": "", + "default": "", + "properties": { + "skip_fastqc": { + "type": "boolean", + "description": "Skip FASTQC or not?" + }, + "skip_fastp": { + "type": "boolean", + "description": "Skip trimming by FASTQP or not?" + }, + "min_trimmed_reads": { + "type": "integer", + "default": 10000, + "description": "Exclude a sample if its reads after trimming are below this number", + "minimum": 0 + }, + "extra_fastp_args": { + "type": "string", + "description": "Extra FASTP arguments" + }, + "save_trimmed": { + "type": "boolean", + "description": "Save FASTQ files after trimming or not?" + }, + "remove_ribo_rna": { + "type": "boolean", + "description": "Remove Ribosomal RNA or not?" + }, + "save_non_ribo_reads": { + "type": "boolean", + "description": "Save FASTQ files after Ribosomal RNA removal or not?" + }, + "ribo_database_manifest": { + "type": "string", + "default": "${projectDir}/assets/rrna-db-defaults.txt", + "description": "Ribosomal RNA fastas listed in a text sheet", + "format": "file-path", + "mimetype": "text/txt" + } + } + }, + "rnaseq_alignment_options": { + "title": "RNAseq alignment options", + "type": "object", + "description": "", + "default": "", + "properties": { + "star_max_intron_length": { + "type": "integer", + "default": 16000, + "minimum": 0, + "description": "Maximum intron length for STAR alignment" + }, + "star_align_extra_args": { + "type": "string", + "description": "EXTRA arguments for STAR" + }, + "star_save_outputs": { + "type": "boolean", + "description": "Save BAM files from STAR or not?" + }, + "save_cat_bam": { + "type": "boolean", + "description": "SAVE a concatenated BAM file per assembly or not?" + } + } + }, + "annotation_options": { + "title": "Annotation options", + "type": "object", + "description": "", + "default": "", + "properties": { + "braker_extra_args": { + "type": "string", + "description": "Extra arguments for BRAKER" + }, + "liftoff_coverage": { + "type": "number", + "default": 0.9, + "minimum": 0, + "maximum": 1, + "description": "Liftoff coverage parameter" + }, + "liftoff_identity": { + "type": "number", + "default": 0.9, + "description": "Liftoff identity parameter" + } + } + }, + "max_job_request_options": { + "title": "Max job request options", + "type": "object", + "fa_icon": "fab fa-acquisitions-incorporated", + "description": "Set the top limit for requested resources for any single job.", + "help_text": "If you are running on a smaller system, a pipeline step requesting more resources than are available may cause the Nextflow to stop the run with an error. These options allow you to cap the maximum resources requested by any single job so that the pipeline will run on your system.\n\nNote that you can not _increase_ the resources requested by any job using these options. For that you will need your own configuration file. See [the nf-core website](https://nf-co.re/usage/configuration) for details.", + "properties": { + "max_cpus": { + "type": "integer", + "description": "Maximum number of CPUs that can be requested for any single job.", + "default": 12, + "fa_icon": "fas fa-microchip", + "hidden": true, + "help_text": "Use to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`" + }, + "max_memory": { + "type": "string", + "description": "Maximum amount of memory that can be requested for any single job.", + "default": "200.GB", + "fa_icon": "fas fa-memory", + "pattern": "^\\d+(\\.\\d+)?\\.?\\s*(K|M|G|T)?B$", + "hidden": true, + "help_text": "Use to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`" + }, + "max_time": { + "type": "string", + "description": "Maximum amount of time that can be requested for any single job.", + "default": "1.day", + "fa_icon": "far fa-clock", + "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", + "hidden": true, + "help_text": "Use to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`" + } + } + }, + "infrastructure_options": { + "title": "Infrastructure options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "", + "help_text": "", + "properties": { + "validationSkipDuplicateCheck": { + "type": "boolean", + "default": true, + "hidden": true + }, + "validationS3PathCheck": { + "type": "boolean", + "default": true, + "hidden": true } } } }, "allOf": [ { - "$ref": "#/defs/input_output_options" + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/repeat_annotation_options" + }, + { + "$ref": "#/definitions/rnaseq_pre_processing_options" + }, + { + "$ref": "#/definitions/rnaseq_alignment_options" + }, + { + "$ref": "#/definitions/annotation_options" + }, + { + "$ref": "#/definitions/max_job_request_options" + }, + { + "$ref": "#/definitions/infrastructure_options" } ] } diff --git a/workflows/pangene.nf b/workflows/pangene.nf index 294dee3..2b30890 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -1,6 +1,5 @@ -include { fromSamplesheet } from 'plugin/nf-validation' -include { validateParams } from '../modules/local/validate_params' -include { idFromFileName; validateFastqMetadata } from '../modules/local/validate_params' +include { fromSamplesheet; paramsSummaryLog } from 'plugin/nf-validation' +include { idFromFileName; validateFastqMetadata } from '../modules/local/utils' include { PREPARE_ASSEMBLY } from '../subworkflows/local/prepare_assembly' include { PREPROCESS_RNASEQ } from '../subworkflows/local/preprocess_rnaseq' include { ALIGN_RNASEQ } from '../subworkflows/local/align_rnaseq' @@ -9,7 +8,7 @@ include { FASTA_BRAKER3 } from '../subworkflows/local/fa include { FASTA_LIFTOFF } from '../subworkflows/local/fasta_liftoff' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions' -validateParams(params) +log.info paramsSummaryLog(workflow) workflow PANGENE { From e9317251c0599a54bf6cf47dbd6f18b333b6da44 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 2 Apr 2024 15:46:53 +1300 Subject: [PATCH 16/40] Added MERGE_ANNOTATIONS sub-workflow --- CHANGELOG.md | 3 +- conf/modules.config | 31 ++-- modules.json | 10 ++ modules/nf-core/gt/gff3/environment.yml | 9 ++ modules/nf-core/gt/gff3/main.nf | 51 +++++++ modules/nf-core/gt/gff3/meta.yml | 48 ++++++ modules/nf-core/gt/gff3/tests/main.nf.test | 61 ++++++++ .../nf-core/gt/gff3/tests/main.nf.test.snap | 72 +++++++++ modules/nf-core/gt/gff3/tests/nextflow.config | 3 + modules/nf-core/gt/gff3/tests/tags.yml | 2 + .../environment.yml | 9 ++ .../agat/spfilterfeaturefromkilllist/main.nf | 53 +++++++ .../agat/spfilterfeaturefromkilllist/meta.yml | 60 ++++++++ .../tests/main.nf.test | 74 +++++++++ .../tests/main.nf.test.snap | 68 +++++++++ .../tests/tags.yml | 2 + subworkflows/local/merge_annotations.nf | 140 ++++++++++++++++++ workflows/pangene.nf | 7 + 18 files changed, 691 insertions(+), 12 deletions(-) create mode 100644 modules/nf-core/gt/gff3/environment.yml create mode 100644 modules/nf-core/gt/gff3/main.nf create mode 100644 modules/nf-core/gt/gff3/meta.yml create mode 100644 modules/nf-core/gt/gff3/tests/main.nf.test create mode 100644 modules/nf-core/gt/gff3/tests/main.nf.test.snap create mode 100644 modules/nf-core/gt/gff3/tests/nextflow.config create mode 100644 modules/nf-core/gt/gff3/tests/tags.yml create mode 100644 modules/pfr/agat/spfilterfeaturefromkilllist/environment.yml create mode 100644 modules/pfr/agat/spfilterfeaturefromkilllist/main.nf create mode 100644 modules/pfr/agat/spfilterfeaturefromkilllist/meta.yml create mode 100644 modules/pfr/agat/spfilterfeaturefromkilllist/tests/main.nf.test create mode 100644 modules/pfr/agat/spfilterfeaturefromkilllist/tests/main.nf.test.snap create mode 100644 modules/pfr/agat/spfilterfeaturefromkilllist/tests/tags.yml create mode 100644 subworkflows/local/merge_annotations.nf diff --git a/CHANGELOG.md b/CHANGELOG.md index 3af66af..e683f5d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 0.2.0+dev - [27-Mar-2024] +## 0.2.0+dev - [02-April-2024] ### `Added` @@ -29,6 +29,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 20. Now using a txt file for `params.external_protein_fastas` 21. Now using nf-validation for `params.liftoff_annotations` 22. Now using nf-validation for all the parameters +23. Added `MERGE_ANNOTATIONS` sub-workflow ### `Fixed` diff --git a/conf/modules.config b/conf/modules.config index 557fc2a..40b811f 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -165,11 +165,6 @@ if(params.liftoff_annotations) { "-a $params.liftoff_coverage", "-s $params.liftoff_identity" ].join(' ').trim() - publishDir = [ - path: { "${params.outdir}/liftoff/${meta.id}" }, - mode: "copy", - saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, - ] } withName: GFFREAD_BEFORE_LIFTOFF { @@ -182,16 +177,30 @@ if(params.liftoff_annotations) { withName: GFFREAD_AFTER_LIFTOFF { ext.args = '--keep-genes --keep-comments --keep-exon-attrs -F' - - publishDir = [ - path: { "${params.outdir}/liftoff" }, - mode: "copy", - saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, - ] } } } +process { + withName: 'VALIDATE_PURGING_BY_AGAT' { + ext.prefix = { "${meta.id}_post_purge" } + } + + withName: 'MERGED_GTF2GFF' { + ext.args = '--keep-genes --keep-comments --keep-exon-attrs -F' + } + + withName: 'VALIDATE_GFF3' { + ext.args = '-tidy' + + publishDir = [ + path: { "${params.outdir}/braker_liftoff_merged" }, + mode: "copy", + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + ] + } +} + process { withName: 'CUSTOM_DUMPSOFTWAREVERSIONS' { publishDir = [ diff --git a/modules.json b/modules.json index 49bab25..d1d6b9c 100644 --- a/modules.json +++ b/modules.json @@ -5,6 +5,11 @@ "git@github.com:PlantandFoodResearch/nxf-modules.git": { "modules": { "pfr": { + "agat/spfilterfeaturefromkilllist": { + "branch": "main", + "git_sha": "7a9a63b7727b0d3b3943f217809059e8116cee2b", + "installed_by": ["modules"] + }, "custom/restoregffids": { "branch": "main", "git_sha": "e9f6bdd634bdbcd52c5568ba82f16176ec06631f", @@ -106,6 +111,11 @@ "git_sha": "b1b959609bda44341120aed1766329909f54b8d0", "installed_by": ["modules"] }, + "gt/gff3": { + "branch": "master", + "git_sha": "3c77ca9aac783e76c3614a06db3bfe4fef619bde", + "installed_by": ["modules"] + }, "gunzip": { "branch": "master", "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", diff --git a/modules/nf-core/gt/gff3/environment.yml b/modules/nf-core/gt/gff3/environment.yml new file mode 100644 index 0000000..8289fb3 --- /dev/null +++ b/modules/nf-core/gt/gff3/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "gt_gff3" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::genometools-genometools=1.6.5" diff --git a/modules/nf-core/gt/gff3/main.nf b/modules/nf-core/gt/gff3/main.nf new file mode 100644 index 0000000..6324a39 --- /dev/null +++ b/modules/nf-core/gt/gff3/main.nf @@ -0,0 +1,51 @@ +process GT_GFF3 { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/genometools-genometools:1.6.5--py310h3db02ab_0': + 'biocontainers/genometools-genometools:1.6.5--py310h3db02ab_0' }" + + input: + tuple val(meta), path(gff3) + + output: + tuple val(meta), path("*.gt.gff3") , emit: gt_gff3 , optional: true + tuple val(meta), path("*.error.log"), emit: error_log , optional: true + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + gt \\ + gff3 \\ + $args \\ + "$gff3" \\ + > "${prefix}.gt.gff3" \\ + 2> >(tee "${prefix}.error.log" >&2) \\ + || echo "Errors from gt-gff3 printed to ${prefix}.error.log" + + if grep -q "gt gff3: error:" "${prefix}.error.log"; then + echo "gt-gff3 failed to parse $gff3" + + rm \\ + "${prefix}.gt.gff3" + else + echo "gt-gff3 successfully parsed $gff3" + + mv \\ + "${prefix}.error.log" \\ + gt_gff3.stderr + fi + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + genometools: \$(gt --version | head -1 | sed 's/gt (GenomeTools) //') + END_VERSIONS + """ +} diff --git a/modules/nf-core/gt/gff3/meta.yml b/modules/nf-core/gt/gff3/meta.yml new file mode 100644 index 0000000..5cecd8d --- /dev/null +++ b/modules/nf-core/gt/gff3/meta.yml @@ -0,0 +1,48 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "gt_gff3" +description: "GenomeTools gt-gff3 utility to parse, possibly transform, and output GFF3 files" +keywords: + - genome + - gff3 + - annotation +tools: + - "gt": + description: "The GenomeTools genome analysis system" + homepage: "https://genometools.org/index.html" + documentation: "https://genometools.org/documentation.html" + tool_dev_url: "https://github.com/genometools/genometools" + doi: "10.1109/TCBB.2013.68" + licence: ["ISC"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gff3: + type: file + description: Input gff3 file + pattern: "*.{gff,gff3}" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test' ]` + - gt_gff3: + type: file + description: Parsed gff3 file produced only if there is no parsing error + pattern: "*.gt.gff3" + - error_log: + type: file + description: Error log if gt-gff3 failed to parse the input gff3 file + pattern: "*.error.log" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@gallvp" +maintainers: + - "@gallvp" diff --git a/modules/nf-core/gt/gff3/tests/main.nf.test b/modules/nf-core/gt/gff3/tests/main.nf.test new file mode 100644 index 0000000..cb44bc8 --- /dev/null +++ b/modules/nf-core/gt/gff3/tests/main.nf.test @@ -0,0 +1,61 @@ +nextflow_process { + + name "Test Process GT_GFF3" + script "../main.nf" + process "GT_GFF3" + config "./nextflow.config" + + tag "modules" + tag "modules_nfcore" + tag "gt" + tag "gt/gff3" + + test("sarscov2-gff3-valid") { + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.gt_gff3 != null }, + { assert process.out.error_log == [] } + ) + } + + } + + test("sarscov2-gff3-invalid") { + when { + process { + """ + input[0] = Channel.of( + '##gff-version 3', + 'chr22\tID=gene:ENSG00000233995;Name=AP000547.1' + ) + .collectFile(name: 'sample.gff3', newLine: true) + .map { file -> [ [ id:'test' ], file ] } + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.gt_gff3 == [] }, + { assert process.out.error_log != null }, + { assert path(process.out.error_log.get(0).get(1)).getText().contains("gt gff3: error:") } + ) + } + } + +} diff --git a/modules/nf-core/gt/gff3/tests/main.nf.test.snap b/modules/nf-core/gt/gff3/tests/main.nf.test.snap new file mode 100644 index 0000000..6e454f1 --- /dev/null +++ b/modules/nf-core/gt/gff3/tests/main.nf.test.snap @@ -0,0 +1,72 @@ +{ + "sarscov2-gff3-invalid": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.error.log:md5,31e6117c516f936ec403f792c732bc76" + ] + ], + "2": [ + "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" + ], + "error_log": [ + [ + { + "id": "test" + }, + "test.error.log:md5,31e6117c516f936ec403f792c732bc76" + ] + ], + "gt_gff3": [ + + ], + "versions": [ + "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" + ] + } + ], + "timestamp": "2023-11-29T10:42:11.408352" + }, + "sarscov2-gff3-valid": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" + ] + ], + "1": [ + + ], + "2": [ + "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" + ], + "error_log": [ + + ], + "gt_gff3": [ + [ + { + "id": "test" + }, + "test.gt.gff3:md5,2ae900237ace415557b8735fac088b85" + ] + ], + "versions": [ + "versions.yml:md5,9753770dd19a2a306dcf16d4aaf049eb" + ] + } + ], + "timestamp": "2023-11-29T10:42:07.817894" + } +} \ No newline at end of file diff --git a/modules/nf-core/gt/gff3/tests/nextflow.config b/modules/nf-core/gt/gff3/tests/nextflow.config new file mode 100644 index 0000000..af56226 --- /dev/null +++ b/modules/nf-core/gt/gff3/tests/nextflow.config @@ -0,0 +1,3 @@ +process { + ext.args = '-tidy -retainids' +} diff --git a/modules/nf-core/gt/gff3/tests/tags.yml b/modules/nf-core/gt/gff3/tests/tags.yml new file mode 100644 index 0000000..0ce15a9 --- /dev/null +++ b/modules/nf-core/gt/gff3/tests/tags.yml @@ -0,0 +1,2 @@ +gt/gff3: + - "modules/nf-core/gt/gff3/**" diff --git a/modules/pfr/agat/spfilterfeaturefromkilllist/environment.yml b/modules/pfr/agat/spfilterfeaturefromkilllist/environment.yml new file mode 100644 index 0000000..b0811b4 --- /dev/null +++ b/modules/pfr/agat/spfilterfeaturefromkilllist/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "agat_spfilterfeaturefromkilllist" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::agat=1.3.3" diff --git a/modules/pfr/agat/spfilterfeaturefromkilllist/main.nf b/modules/pfr/agat/spfilterfeaturefromkilllist/main.nf new file mode 100644 index 0000000..ffb91d1 --- /dev/null +++ b/modules/pfr/agat/spfilterfeaturefromkilllist/main.nf @@ -0,0 +1,53 @@ +process AGAT_SPFILTERFEATUREFROMKILLLIST { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/agat:1.3.3--pl5321hdfd78af_0': + 'biocontainers/agat:1.3.3--pl5321hdfd78af_0' }" + + input: + tuple val(meta), path(gff) + path kill_list + path config + + output: + tuple val(meta), path("*.gff"), emit: gff + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def config_param = config ? "--config $config" : '' + if( "$gff" == "${prefix}.gff" ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + agat_sp_filter_feature_from_kill_list.pl \\ + --gff $gff \\ + --kill_list $kill_list \\ + $config_param \\ + $args \\ + --output "${prefix}.gff" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + agat: \$(agat_sp_filter_feature_from_kill_list.pl -h | sed -n 's/.*(AGAT) - Version: \\(.*\\) .*/\\1/p') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + if( "$gff" == "${prefix}.gff" ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + touch "${prefix}.gff" + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + agat: \$(agat_sp_filter_feature_from_kill_list.pl -h | sed -n 's/.*(AGAT) - Version: \\(.*\\) .*/\\1/p') + END_VERSIONS + """ +} diff --git a/modules/pfr/agat/spfilterfeaturefromkilllist/meta.yml b/modules/pfr/agat/spfilterfeaturefromkilllist/meta.yml new file mode 100644 index 0000000..d408fe7 --- /dev/null +++ b/modules/pfr/agat/spfilterfeaturefromkilllist/meta.yml @@ -0,0 +1,60 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "agat_spfilterfeaturefromkilllist" +description: | + The script aims to remove features based on a kill list. The default behaviour is to look at the features's ID. + If the feature has an ID (case insensitive) listed among the kill list it will be removed. /!\ Removing a level1 + or level2 feature will automatically remove all linked subfeatures, and removing all children of a feature will + automatically remove this feature too. +keywords: + - genomics + - gff + - remove + - feature +tools: + - "agat": + description: "Another Gff Analysis Toolkit (AGAT). Suite of tools to handle gene annotations in any GTF/GFF format." + homepage: "https://agat.readthedocs.io/en/latest/" + documentation: "https://agat.readthedocs.io/en/latest/tools/agat_sp_filter_feature_from_kill_list.html" + tool_dev_url: "https://github.com/NBISweden/AGAT" + doi: "10.5281/zenodo.3552717" + licence: ["GPL v3"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ]` + - gff: + type: file + description: Input GFF3 file that will be read + pattern: "*.{gff,gff3}" + - kill_list: + type: file + description: Kill list. One value per line. + pattern: "*.txt" + - config: + type: file + description: | + Input agat config file. By default AGAT takes as input agat_config.yaml file from the working directory if any, otherwise it takes the orignal agat_config.yaml shipped with AGAT. To get the agat_config.yaml locally type: "agat config --expose". The --config option gives you the possibility to use your own AGAT config file (located elsewhere or named differently). + pattern: "*.yaml" + +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1', single_end:false ] + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - gff: + type: file + description: Output GFF file. + pattern: "*.gff" + +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/agat/spfilterfeaturefromkilllist/tests/main.nf.test b/modules/pfr/agat/spfilterfeaturefromkilllist/tests/main.nf.test new file mode 100644 index 0000000..8f04e58 --- /dev/null +++ b/modules/pfr/agat/spfilterfeaturefromkilllist/tests/main.nf.test @@ -0,0 +1,74 @@ +nextflow_process { + + name "Test Process AGAT_SPFILTERFEATUREFROMKILLLIST" + script "../main.nf" + process "AGAT_SPFILTERFEATUREFROMKILLLIST" + + tag "modules" + tag "modules_nfcore" + tag "agat" + tag "agat/spfilterfeaturefromkilllist" + + test("sarscov2-genome_gff3") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + + def kill_list = "unknown_transcript_1" + def kill_list_file = new File('kill.list.txt') + kill_list_file.text = kill_list + + input[1] = kill_list_file.toPath() + + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("sarscov2-genome_gff3-stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + + def kill_list = "unknown_transcript_1" + def kill_list_file = new File('kill.list.txt') + kill_list_file.text = kill_list + + input[1] = kill_list_file.toPath() + + input[2] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/pfr/agat/spfilterfeaturefromkilllist/tests/main.nf.test.snap b/modules/pfr/agat/spfilterfeaturefromkilllist/tests/main.nf.test.snap new file mode 100644 index 0000000..bbc8cea --- /dev/null +++ b/modules/pfr/agat/spfilterfeaturefromkilllist/tests/main.nf.test.snap @@ -0,0 +1,68 @@ +{ + "sarscov2-genome_gff3-stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gff:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,ace24108f514da465e068372b18d4651" + ], + "gff": [ + [ + { + "id": "test" + }, + "test.gff:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,ace24108f514da465e068372b18d4651" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-02T13:29:56.638311" + }, + "sarscov2-genome_gff3": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gff:md5,df19e1b84ba6f691d20c72b397c88abf" + ] + ], + "1": [ + "versions.yml:md5,ace24108f514da465e068372b18d4651" + ], + "gff": [ + [ + { + "id": "test" + }, + "test.gff:md5,df19e1b84ba6f691d20c72b397c88abf" + ] + ], + "versions": [ + "versions.yml:md5,ace24108f514da465e068372b18d4651" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-02T13:11:42.236263" + } +} \ No newline at end of file diff --git a/modules/pfr/agat/spfilterfeaturefromkilllist/tests/tags.yml b/modules/pfr/agat/spfilterfeaturefromkilllist/tests/tags.yml new file mode 100644 index 0000000..2ab17b0 --- /dev/null +++ b/modules/pfr/agat/spfilterfeaturefromkilllist/tests/tags.yml @@ -0,0 +1,2 @@ +agat/spfilterfeaturefromkilllist: + - "modules/pfr/agat/spfilterfeaturefromkilllist/**" diff --git a/subworkflows/local/merge_annotations.nf b/subworkflows/local/merge_annotations.nf new file mode 100644 index 0000000..a24eded --- /dev/null +++ b/subworkflows/local/merge_annotations.nf @@ -0,0 +1,140 @@ +include { GFFCOMPARE as COMPARE_BRAKER_TO_LIFTOFF } from '../../modules/nf-core/gffcompare/main' +include { AGAT_SPFILTERFEATUREFROMKILLLIST } from '../../modules/pfr/agat/spfilterfeaturefromkilllist/main' +include { GFFCOMPARE as VALIDATE_PURGING_BY_AGAT } from '../../modules/nf-core/gffcompare/main' +include { GFFCOMPARE as MERGE_BRAKER_LIFTOFF } from '../../modules/nf-core/gffcompare/main' +include { GFFREAD as MERGED_GTF2GFF } from '../../modules/nf-core/gffread/main' +include { GT_GFF3 as VALIDATE_GFF3 } from '../../modules/nf-core/gt/gff3/main' + +workflow MERGE_ANNOTATIONS { + take: + braker_gff3 // [ meta, gff3 ] + liftoff_gff3 // [ meta, gff3 ] + + main: + ch_versions = Channel.empty() + + // MODULE: GFFCOMPARE as COMPARE_BRAKER_TO_LIFTOFF + ch_comparison_inputs = braker_gff3 + | join(liftoff_gff3) + + + COMPARE_BRAKER_TO_LIFTOFF ( + ch_comparison_inputs.map { meta, braker, liftoff -> [ meta, braker ] }, + [ [], [], [] ], + ch_comparison_inputs.map { meta, braker, liftoff -> [ meta, liftoff ] }, + ) + + ch_tracking = COMPARE_BRAKER_TO_LIFTOFF.out.tracking + ch_versions = ch_versions.mix(COMPARE_BRAKER_TO_LIFTOFF.out.versions.first()) + + // COLLECTFILE: Transcript level kill list + ch_kill_list = ch_tracking + | map { meta, tracking -> + + def kept_lines = tracking.readLines() + .findAll { line -> + def cols = line.split('\t') + + ( cols[3] != 'u' ) && ( cols[3] != 'p' ) + } + + def tx_kill_list = kept_lines + .collect { line -> + def cols = line.split('\t') + + def matched = cols[4] =~ /q1:([^\|]+)\|([^\|]+)/ + + matched[0][2].trim() + } + + [ "${meta.id}.kill.list.txt" ] + tx_kill_list + } + | collectFile(newLine: true) + | map { file -> + [ [ id: file.baseName.replace(".kill.list", "") ], file ] + } + + // MODULE: AGAT_SPFILTERFEATUREFROMKILLLIST + ch_agat_kill_inputs = braker_gff3 + | join(ch_kill_list) + + + AGAT_SPFILTERFEATUREFROMKILLLIST( + ch_agat_kill_inputs.map { meta, gff, kill -> [ meta, gff ] }, + ch_agat_kill_inputs.map { meta, gff, kill -> kill }, + [] // default config + ) + + ch_braker_purged = AGAT_SPFILTERFEATUREFROMKILLLIST.out.gff + ch_versions = ch_versions.mix(AGAT_SPFILTERFEATUREFROMKILLLIST.out.versions.first()) + + // MODULE: GFFCOMPARE as VALIDATE_PURGING_BY_AGAT + ch_validation_inputs = ch_braker_purged + | join(liftoff_gff3) + + VALIDATE_PURGING_BY_AGAT( + ch_validation_inputs.map { meta, braker, liftoff -> [ meta, braker ] }, + [ [], [], [] ], + ch_validation_inputs.map { meta, braker, liftoff -> [ meta, liftoff ] } + ) + + ch_validation_tracking = VALIDATE_PURGING_BY_AGAT.out.tracking + ch_versions = ch_versions.mix(VALIDATE_PURGING_BY_AGAT.out.versions.first()) + + // VALIDATE: All gffcompare codes in [ 'u', 'p' ] + ch_validation_output = ch_validation_tracking + | map { meta, tracking -> + + def tx_list = tracking.readLines() + .collect { line -> + def cols = line.split('\t') + def matched = cols[4] =~ /q1:([^\|]+)\|([^\|]+)/ + def tx_id = matched[0][2].trim() + def code = cols[3] + + if ( code != 'u' && code != 'p' ) { + log.warn "In ${meta.id}, transcript $tx_id has code ${code}." + + " This issue can break the downstream logic in the pipeline." + + " Please contact the devs!" + } + + [ tx_id, code ] + } + + [ meta, tx_list ] + } + + // MODULE: GFFCOMPARE as MERGE_BRAKER_LIFTOFF + ch_merge_inputs = ch_braker_purged + | join(liftoff_gff3) + MERGE_BRAKER_LIFTOFF( + ch_merge_inputs.map { meta, braker, liftoff -> [ meta, [ braker, liftoff ] ] }, + [ [], [], [] ], + [ [], [] ] + ) + + ch_versions = ch_versions.mix(MERGE_BRAKER_LIFTOFF.out.versions.first()) + + // MODULE: GFFREAD as MERGED_GTF2GFF + ch_merged_gtf = MERGE_BRAKER_LIFTOFF.out.combined_gtf + | map { meta, gtf -> + [ gtf.baseName, meta, gtf ] + } // For meta insertion later, remove when GFFREAD has meta + MERGED_GTF2GFF ( ch_merged_gtf.map { name, meta, gtf -> gtf } ) + + ch_merged_gff = MERGED_GTF2GFF.out.gffread_gff + | map { gff -> [ gff.baseName - '.gffread', gff ] } + | join(ch_merged_gtf) + | map { fid, gffread_gff, meta, gtf -> [ meta, gffread_gff ] } + + ch_versions = ch_versions.mix(MERGED_GTF2GFF.out.versions.first()) + + // MODULE: GT_GFF3 as VALIDATE_GFF3 + VALIDATE_GFF3 ( ch_merged_gff ) + + ch_versions = ch_versions.mix(VALIDATE_GFF3.out.versions.first()) + + emit: + merged_gff = VALIDATE_GFF3.out.gt_gff3 + versions = ch_versions +} diff --git a/workflows/pangene.nf b/workflows/pangene.nf index 2b30890..bffaf3a 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -6,6 +6,7 @@ include { ALIGN_RNASEQ } from '../subworkflows/local/al include { PREPARE_EXT_PROTS } from '../subworkflows/local/prepare_ext_prots' include { FASTA_BRAKER3 } from '../subworkflows/local/fasta_braker3' include { FASTA_LIFTOFF } from '../subworkflows/local/fasta_liftoff' +include { MERGE_ANNOTATIONS } from '../subworkflows/local/merge_annotations' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions' log.info paramsSummaryLog(workflow) @@ -196,6 +197,12 @@ workflow PANGENE { ch_liftoff_gff3 = FASTA_LIFTOFF.out.gff3 ch_versions = ch_versions.mix(FASTA_LIFTOFF.out.versions) + // SUBWORKFLOW: MERGE_ANNOTATIONS + MERGE_ANNOTATIONS ( ch_braker_gff3, ch_liftoff_gff3 ) + + ch_merged_gff = MERGE_ANNOTATIONS.out.merged_gff + ch_versions = ch_versions.mix(MERGE_ANNOTATIONS.out.versions) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From 40f5cc5dce36698dd3bc210ac9e77784274172d9 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 2 Apr 2024 17:34:57 +1300 Subject: [PATCH 17/40] Now merging with agat/spmergeannotations --- conf/modules.config | 17 ++--- modules.json | 5 ++ .../agat/spmergeannotations/environment.yml | 9 +++ modules/pfr/agat/spmergeannotations/main.nf | 53 ++++++++++++++ modules/pfr/agat/spmergeannotations/meta.yml | 54 ++++++++++++++ .../spmergeannotations/tests/main.nf.test | 71 +++++++++++++++++++ .../tests/main.nf.test.snap | 47 ++++++++++++ .../agat/spmergeannotations/tests/tags.yml | 2 + subworkflows/local/fasta_liftoff.nf | 47 ++++-------- subworkflows/local/merge_annotations.nf | 37 +++------- 10 files changed, 268 insertions(+), 74 deletions(-) create mode 100644 modules/pfr/agat/spmergeannotations/environment.yml create mode 100644 modules/pfr/agat/spmergeannotations/main.nf create mode 100644 modules/pfr/agat/spmergeannotations/meta.yml create mode 100644 modules/pfr/agat/spmergeannotations/tests/main.nf.test create mode 100644 modules/pfr/agat/spmergeannotations/tests/main.nf.test.snap create mode 100644 modules/pfr/agat/spmergeannotations/tests/tags.yml diff --git a/conf/modules.config b/conf/modules.config index 40b811f..344285d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -171,12 +171,8 @@ if(params.liftoff_annotations) { ext.args = '--no-pseudo --keep-genes' } - withName: COMBINE_LIFTOFF_ANNOTATIONS { - ext.args = '-T' - } - - withName: GFFREAD_AFTER_LIFTOFF { - ext.args = '--keep-genes --keep-comments --keep-exon-attrs -F' + withName: MERGE_LIFTOFF_ANNOTATIONS { + ext.prefix = { "${meta.id}.merged.liftoffs" } } } } @@ -186,15 +182,12 @@ process { ext.prefix = { "${meta.id}_post_purge" } } - withName: 'MERGED_GTF2GFF' { - ext.args = '--keep-genes --keep-comments --keep-exon-attrs -F' - } + withName: 'MERGE_BRAKER_LIFTOFF' { - withName: 'VALIDATE_GFF3' { - ext.args = '-tidy' + ext.prefix = { "${meta.id}.braker.liftoff.merged" } publishDir = [ - path: { "${params.outdir}/braker_liftoff_merged" }, + path: { "${params.outdir}/gff" }, mode: "copy", saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, ] diff --git a/modules.json b/modules.json index d1d6b9c..d02a9ec 100644 --- a/modules.json +++ b/modules.json @@ -10,6 +10,11 @@ "git_sha": "7a9a63b7727b0d3b3943f217809059e8116cee2b", "installed_by": ["modules"] }, + "agat/spmergeannotations": { + "branch": "main", + "git_sha": "e2ee3129519098451ef60f8a8ca3b9efcecf2d69", + "installed_by": ["modules"] + }, "custom/restoregffids": { "branch": "main", "git_sha": "e9f6bdd634bdbcd52c5568ba82f16176ec06631f", diff --git a/modules/pfr/agat/spmergeannotations/environment.yml b/modules/pfr/agat/spmergeannotations/environment.yml new file mode 100644 index 0000000..6df7aea --- /dev/null +++ b/modules/pfr/agat/spmergeannotations/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "agat_spmergeannotations" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::agat=1.3.3" diff --git a/modules/pfr/agat/spmergeannotations/main.nf b/modules/pfr/agat/spmergeannotations/main.nf new file mode 100644 index 0000000..7738ac5 --- /dev/null +++ b/modules/pfr/agat/spmergeannotations/main.nf @@ -0,0 +1,53 @@ +process AGAT_SPMERGEANNOTATIONS { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/agat:1.3.3--pl5321hdfd78af_0': + 'biocontainers/agat:1.3.3--pl5321hdfd78af_0' }" + + input: + tuple val(meta), path(gffs) + path config + + output: + tuple val(meta), path("*.gff") , emit: gff + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def config_param = config ? "--config $config" : '' + def gff_param = "$gffs".split(' ').collect { "--gff $it" }.join(' ') + def file_names = gffs.collect { "$it" } + if ( file_names.contains ( "${prefix}.gff" ) ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + agat_sp_merge_annotations.pl \\ + $gff_param \\ + $config_param \\ + $args \\ + --output ${prefix}.gff + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + agat: \$(agat_sp_merge_annotations.pl -h | sed -n 's/.*(AGAT) - Version: \\(.*\\) .*/\\1/p') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + def file_names = gffs.collect { "$it" } + if ( file_names.contains ( "${prefix}.gff" ) ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + touch ${prefix}.gff + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + agat: \$(agat_sp_merge_annotations.pl -h | sed -n 's/.*(AGAT) - Version: \\(.*\\) .*/\\1/p') + END_VERSIONS + """ +} diff --git a/modules/pfr/agat/spmergeannotations/meta.yml b/modules/pfr/agat/spmergeannotations/meta.yml new file mode 100644 index 0000000..afa9ddd --- /dev/null +++ b/modules/pfr/agat/spmergeannotations/meta.yml @@ -0,0 +1,54 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "agat_spmergeannotations" +description: | + This script merge different gff annotation files in one. It uses the AGAT parser that takes care of duplicated names and fixes other oddities met in those files. +keywords: + - genomics + - gff + - merge + - combine +tools: + - "agat": + description: "Another Gff Analysis Toolkit (AGAT). Suite of tools to handle gene annotations in any GTF/GFF format." + homepage: "https://agat.readthedocs.io/en/latest/" + documentation: "https://agat.readthedocs.io/en/latest/tools/agat_sp_merge_annotations.html" + tool_dev_url: "https://github.com/NBISweden/AGAT" + doi: "10.5281/zenodo.3552717" + licence: ["GPL v3"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - gffs: + type: list + description: A list of GFFs to merge + pattern: "[ *.{gff,gff3} ]" + - config: + type: file + description: | + Input agat config file. By default AGAT takes as input agat_config.yaml file from the working directory if any, + otherwise it takes the orignal agat_config.yaml shipped with AGAT. To get the agat_config.yaml + locally type: "agat config --expose". The --config option gives you the possibility to use your + own AGAT config file (located elsewhere or named differently). + pattern: "*.yaml" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - gff: + type: file + description: Output GFF file. + pattern: "*.gff" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/agat/spmergeannotations/tests/main.nf.test b/modules/pfr/agat/spmergeannotations/tests/main.nf.test new file mode 100644 index 0000000..78f2eba --- /dev/null +++ b/modules/pfr/agat/spmergeannotations/tests/main.nf.test @@ -0,0 +1,71 @@ +nextflow_process { + + name "Test Process AGAT_SPMERGEANNOTATIONS" + script "../main.nf" + process "AGAT_SPMERGEANNOTATIONS" + + tag "modules" + tag "modules_nfcore" + tag "agat" + tag "agat/spmergeannotations" + + test("candidatus_portiera_aleyrodidarum-multi_gffs") { + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + [ + file(params.test_data['candidatus_portiera_aleyrodidarum']['genome']['test1_gff'], checkIfExists: true), + file(params.test_data['candidatus_portiera_aleyrodidarum']['genome']['test2_gff'], checkIfExists: true), + file(params.test_data['candidatus_portiera_aleyrodidarum']['genome']['test3_gff'], checkIfExists: true), + ] + ] + + input[1] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.gff[0][1]).text.contains('AGAT gene') }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + + test("candidatus_portiera_aleyrodidarum-multi_gffs-stub") { + + options '-stub' + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + [ + file(params.test_data['candidatus_portiera_aleyrodidarum']['genome']['test1_gff'], checkIfExists: true), + file(params.test_data['candidatus_portiera_aleyrodidarum']['genome']['test2_gff'], checkIfExists: true), + file(params.test_data['candidatus_portiera_aleyrodidarum']['genome']['test3_gff'], checkIfExists: true), + ] + ] + + input[1] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/pfr/agat/spmergeannotations/tests/main.nf.test.snap b/modules/pfr/agat/spmergeannotations/tests/main.nf.test.snap new file mode 100644 index 0000000..c7e2154 --- /dev/null +++ b/modules/pfr/agat/spmergeannotations/tests/main.nf.test.snap @@ -0,0 +1,47 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,0ae449590befbaac9269ad8a7a84b66d" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-02T17:08:15.459625" + }, + "candidatus_portiera_aleyrodidarum-multi_gffs-stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gff:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + "versions.yml:md5,0ae449590befbaac9269ad8a7a84b66d" + ], + "gff": [ + [ + { + "id": "test" + }, + "test.gff:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,0ae449590befbaac9269ad8a7a84b66d" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-02T17:08:20.581403" + } +} \ No newline at end of file diff --git a/modules/pfr/agat/spmergeannotations/tests/tags.yml b/modules/pfr/agat/spmergeannotations/tests/tags.yml new file mode 100644 index 0000000..7d9b839 --- /dev/null +++ b/modules/pfr/agat/spmergeannotations/tests/tags.yml @@ -0,0 +1,2 @@ +agat/spmergeannotations: + - "modules/pfr/agat/spmergeannotations/**" diff --git a/subworkflows/local/fasta_liftoff.nf b/subworkflows/local/fasta_liftoff.nf index 17dcf8b..8722a37 100644 --- a/subworkflows/local/fasta_liftoff.nf +++ b/subworkflows/local/fasta_liftoff.nf @@ -1,9 +1,8 @@ -include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/gunzip/main' -include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/gunzip/main' -include { GFFREAD as GFFREAD_BEFORE_LIFTOFF } from '../../modules/nf-core/gffread/main' -include { LIFTOFF } from '../../modules/nf-core/liftoff/main' -include { GFFCOMPARE as COMBINE_LIFTOFF_ANNOTATIONS } from '../../modules/nf-core/gffcompare/main' -include { GFFREAD as GFFREAD_AFTER_LIFTOFF } from '../../modules/nf-core/gffread/main' +include { GUNZIP as GUNZIP_FASTA } from '../../modules/nf-core/gunzip/main' +include { GUNZIP as GUNZIP_GFF } from '../../modules/nf-core/gunzip/main' +include { GFFREAD as GFFREAD_BEFORE_LIFTOFF } from '../../modules/nf-core/gffread/main' +include { LIFTOFF } from '../../modules/nf-core/liftoff/main' +include { AGAT_SPMERGEANNOTATIONS as MERGE_LIFTOFF_ANNOTATIONS } from '../../modules/pfr/agat/spmergeannotations/main' workflow FASTA_LIFTOFF { take: @@ -95,40 +94,22 @@ workflow FASTA_LIFTOFF { ch_versions = ch_versions.mix(LIFTOFF.out.versions.first()) - // MODULE: GFFCOMPARE as COMBINE_LIFTOFF_ANNOTATIONS - ch_gffcompare_inputs = ch_liftoff_gff3 + // MODULE: AGAT_SPMERGEANNOTATIONS as MERGE_LIFTOFF_ANNOTATIONS + ch_merge_inputs = ch_liftoff_gff3 | branch { meta, list_polished -> one: list_polished.size() == 1 many: list_polished.size() > 1 } - COMBINE_LIFTOFF_ANNOTATIONS( - ch_gffcompare_inputs.many, - [ [], [], [] ], - [ [], [] ], + MERGE_LIFTOFF_ANNOTATIONS( + ch_merge_inputs.many, + [] ) - ch_combined_gtf = COMBINE_LIFTOFF_ANNOTATIONS.out.combined_gtf - ch_versions = ch_versions.mix(COMBINE_LIFTOFF_ANNOTATIONS.out.versions.first()) - - // MODULE: GFFREAD as GFFREAD_AFTER_LIFTOFF - ch_post_gffread_inputs = ch_combined_gtf - | map { meta, gtf -> - [ gtf.baseName, meta, gtf ] - } // For meta insertion later, remove when GFFREAD has meta - - GFFREAD_AFTER_LIFTOFF ( ch_post_gffread_inputs.map { name, meta, gtf -> gtf } ) - - ch_combined_gff3 = GFFREAD_AFTER_LIFTOFF.out.gffread_gff - | map { gff -> [ gff.baseName - '.gffread', gff ] } - | join(ch_post_gffread_inputs) - | map { fid, gffread_gff, meta, gtf -> [ meta, gffread_gff ] } - // meta insertion - | mix(ch_gffcompare_inputs.one) - - ch_versions = ch_versions.mix(GFFREAD_AFTER_LIFTOFF.out.versions.first()) + ch_merged_gff = MERGE_LIFTOFF_ANNOTATIONS.out.gff.mix(ch_merge_inputs.one) + ch_versions = ch_versions.mix(MERGE_LIFTOFF_ANNOTATIONS.out.versions.first()) emit: - gff3 = ch_combined_gff3 // [ meta, gff3 ] - versions = ch_versions // [ versions.yml ] + gff3 = ch_merged_gff // [ meta, gff3 ] + versions = ch_versions // [ versions.yml ] } diff --git a/subworkflows/local/merge_annotations.nf b/subworkflows/local/merge_annotations.nf index a24eded..55c6175 100644 --- a/subworkflows/local/merge_annotations.nf +++ b/subworkflows/local/merge_annotations.nf @@ -1,9 +1,7 @@ -include { GFFCOMPARE as COMPARE_BRAKER_TO_LIFTOFF } from '../../modules/nf-core/gffcompare/main' -include { AGAT_SPFILTERFEATUREFROMKILLLIST } from '../../modules/pfr/agat/spfilterfeaturefromkilllist/main' -include { GFFCOMPARE as VALIDATE_PURGING_BY_AGAT } from '../../modules/nf-core/gffcompare/main' -include { GFFCOMPARE as MERGE_BRAKER_LIFTOFF } from '../../modules/nf-core/gffcompare/main' -include { GFFREAD as MERGED_GTF2GFF } from '../../modules/nf-core/gffread/main' -include { GT_GFF3 as VALIDATE_GFF3 } from '../../modules/nf-core/gt/gff3/main' +include { GFFCOMPARE as COMPARE_BRAKER_TO_LIFTOFF } from '../../modules/nf-core/gffcompare/main' +include { AGAT_SPFILTERFEATUREFROMKILLLIST } from '../../modules/pfr/agat/spfilterfeaturefromkilllist/main' +include { GFFCOMPARE as VALIDATE_PURGING_BY_AGAT } from '../../modules/nf-core/gffcompare/main' +include { AGAT_SPMERGEANNOTATIONS as MERGE_BRAKER_LIFTOFF } from '../../modules/pfr/agat/spmergeannotations/main' workflow MERGE_ANNOTATIONS { take: @@ -104,37 +102,18 @@ workflow MERGE_ANNOTATIONS { [ meta, tx_list ] } - // MODULE: GFFCOMPARE as MERGE_BRAKER_LIFTOFF + // MODULE: AGAT_SPMERGEANNOTATIONS as MERGE_BRAKER_LIFTOFF ch_merge_inputs = ch_braker_purged | join(liftoff_gff3) MERGE_BRAKER_LIFTOFF( ch_merge_inputs.map { meta, braker, liftoff -> [ meta, [ braker, liftoff ] ] }, - [ [], [], [] ], - [ [], [] ] + [] ) + ch_merged_gff = MERGE_BRAKER_LIFTOFF.out.gff ch_versions = ch_versions.mix(MERGE_BRAKER_LIFTOFF.out.versions.first()) - // MODULE: GFFREAD as MERGED_GTF2GFF - ch_merged_gtf = MERGE_BRAKER_LIFTOFF.out.combined_gtf - | map { meta, gtf -> - [ gtf.baseName, meta, gtf ] - } // For meta insertion later, remove when GFFREAD has meta - MERGED_GTF2GFF ( ch_merged_gtf.map { name, meta, gtf -> gtf } ) - - ch_merged_gff = MERGED_GTF2GFF.out.gffread_gff - | map { gff -> [ gff.baseName - '.gffread', gff ] } - | join(ch_merged_gtf) - | map { fid, gffread_gff, meta, gtf -> [ meta, gffread_gff ] } - - ch_versions = ch_versions.mix(MERGED_GTF2GFF.out.versions.first()) - - // MODULE: GT_GFF3 as VALIDATE_GFF3 - VALIDATE_GFF3 ( ch_merged_gff ) - - ch_versions = ch_versions.mix(VALIDATE_GFF3.out.versions.first()) - emit: - merged_gff = VALIDATE_GFF3.out.gt_gff3 + merged_gff = ch_merged_gff versions = ch_versions } From 47bb3ed1152346713958ffaabe729f047a415d7a Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 10 Apr 2024 10:30:45 +1200 Subject: [PATCH 18/40] Added GFF_EGGNOGMAPPER sub-workflow --- CHANGELOG.md | 4 +- TODO.md | 16 - conf/modules.config | 20 +- local_pangene | 4 +- modules.json | 5 + modules/nf-core/eggnogmapper/environment.yml | 7 + modules/nf-core/eggnogmapper/main.nf | 68 +++++ modules/nf-core/eggnogmapper/meta.yml | 62 ++++ .../nf-core/eggnogmapper/tests/main.nf.test | 56 ++++ .../eggnogmapper/tests/main.nf.test.snap | 15 + modules/nf-core/eggnogmapper/tests/tags.yml | 2 + modules/nf-core/gffread/environment.yml | 2 +- modules/nf-core/gffread/main.nf | 51 +++- modules/nf-core/gffread/meta.yml | 21 +- modules/nf-core/gffread/tests/main.nf.test | 178 ++++++++++- .../nf-core/gffread/tests/main.nf.test.snap | 276 +++++++++++++++++- .../gffread/tests/nextflow-fasta.config | 5 + nextflow.config | 3 + nextflow_schema.json | 10 + subworkflows/local/fasta_liftoff.nf | 12 +- subworkflows/local/gff_eggnogmapper.nf | 42 +++ subworkflows/local/merge_annotations.nf | 5 +- workflows/pangene.nf | 11 + 23 files changed, 804 insertions(+), 71 deletions(-) create mode 100644 modules/nf-core/eggnogmapper/environment.yml create mode 100644 modules/nf-core/eggnogmapper/main.nf create mode 100644 modules/nf-core/eggnogmapper/meta.yml create mode 100644 modules/nf-core/eggnogmapper/tests/main.nf.test create mode 100644 modules/nf-core/eggnogmapper/tests/main.nf.test.snap create mode 100644 modules/nf-core/eggnogmapper/tests/tags.yml create mode 100644 modules/nf-core/gffread/tests/nextflow-fasta.config create mode 100644 subworkflows/local/gff_eggnogmapper.nf diff --git a/CHANGELOG.md b/CHANGELOG.md index e683f5d..0670fac 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 0.2.0+dev - [02-April-2024] +## 0.2.0+dev - [09-April-2024] ### `Added` @@ -30,6 +30,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 21. Now using nf-validation for `params.liftoff_annotations` 22. Now using nf-validation for all the parameters 23. Added `MERGE_ANNOTATIONS` sub-workflow +24. Added `GFF_EGGNOGMAPPER` sub-workflow +25. Now using a custom version of `GFFREAD` which supports `meta` and `fasta` ### `Fixed` diff --git a/TODO.md b/TODO.md index 59f5177..8e4fe71 100644 --- a/TODO.md +++ b/TODO.md @@ -1,19 +1,3 @@ -- [ ] Add --eval=reference.gtf -- [ ] From Ross regarding post-processing: - -> [9:49 am] Ross Crowhurst -> Here is an easy one: BLATSp vs swissprot & Arabidpsis and check query is with set thresholds of reference - if so accept; If not move to BLASTp vs Uniref90 or Refeq (or some other predetermined model species) - same deal accept if within threshold limits. Else BLASTn of cds vs NCBI nt (really scrapping the bottom of the barrel here). If not a hit to anything then chances are its garbage and should be removed. Some ppl might try to claim its a unique protein to the genotype but in 20 years I have never seen one of those be supported - mostly this category is garbage. The screen agains NCBI nt also assists to classify "bits" as well retroposonss etc. Idea being you want to remove garbage predictions - as this does take time you can see why some papers just filter out by size. - - [ ] From Cecilia: > https://github.com/zhaotao1987/SynNet-Pipeline - -- [ ] From Ross: - -> https://www.biorxiv.org/content/10.1101/096529v2.full.pdf - -- Mib finder, eggnog, blastp against TAIR and uniprot (Wait) -- entap to merge (Wait) -- trinity and PASA + StringTie2 -> Evigene (Do) -- othrofinder paper -- gffcompre on braker and liftoff diff --git a/conf/modules.config b/conf/modules.config index 344285d..fe3d2f8 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -157,7 +157,6 @@ process { if(params.liftoff_annotations) { process { withName: LIFTOFF { - ext.args = ' ' ext.args = [ '-exclude_partial', '-copies', @@ -194,6 +193,25 @@ process { } } +process { + withName: GFF2FASTA_FOR_EGGNOGMAPPER { + ext.args = '-y' + } + + withName: EGGNOGMAPPER { + ext.args = [ + '--evalue 0.00001', + '--pident 35', + '--mp_start_method fork', + "--itype proteins", + params.eggnogmapper_tax_scope ? "--tax_scope ${params.eggnogmapper_tax_scope}" : '', + '--go_evidence all', + '--report_orthologs' + + ].join(' ').trim() + } +} + process { withName: 'CUSTOM_DUMPSOFTWAREVERSIONS' { publishDir = [ diff --git a/local_pangene b/local_pangene index 255edb9..d242a68 100755 --- a/local_pangene +++ b/local_pangene @@ -17,4 +17,6 @@ nextflow \ -profile local,docker \ -resume \ $stub \ - -params-file conf/test_params.json + -params-file conf/test_params.json \ + --eggnogmapper_db_dir ../../dbs/emapperdb/5.0.2 \ + --eggnogmapper_tax_scope 33090 diff --git a/modules.json b/modules.json index d02a9ec..57cbcfc 100644 --- a/modules.json +++ b/modules.json @@ -91,6 +91,11 @@ "git_sha": "de45447d060b8c8b98575bc637a4a575fd0638e1", "installed_by": ["modules"] }, + "eggnogmapper": { + "branch": "master", + "git_sha": "9be0d5f9aeb31bded6780b5b589de7158ccf2c7b", + "installed_by": ["modules"] + }, "fastavalidator": { "branch": "master", "git_sha": "89ff95427f695086369d7927a3c17cea2a37a382", diff --git a/modules/nf-core/eggnogmapper/environment.yml b/modules/nf-core/eggnogmapper/environment.yml new file mode 100644 index 0000000..f4fb6fd --- /dev/null +++ b/modules/nf-core/eggnogmapper/environment.yml @@ -0,0 +1,7 @@ +name: eggnogmapper +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::eggnog-mapper=2.1.12 diff --git a/modules/nf-core/eggnogmapper/main.nf b/modules/nf-core/eggnogmapper/main.nf new file mode 100644 index 0000000..134451d --- /dev/null +++ b/modules/nf-core/eggnogmapper/main.nf @@ -0,0 +1,68 @@ +process EGGNOGMAPPER { + tag "$meta.id" + label 'process_high' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/eggnog-mapper:2.1.12--pyhdfd78af_0': + 'biocontainers/eggnog-mapper:2.1.12--pyhdfd78af_0' }" + + input: + tuple val(meta), path(fasta) + path(eggnog_db) + path(eggnog_data_dir) + tuple val(meta2), path(eggnog_diamond_db) + + output: + tuple val(meta), path("*.emapper.annotations") , emit: annotations + tuple val(meta), path("*.emapper.seed_orthologs"), emit: orthologs + tuple val(meta), path("*.emapper.hits") , emit: hits + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def is_compressed = fasta.name.endsWith(".gz") + def fasta_name = fasta.name.replace(".gz", "") + def dbmem = task.memory.toMega() > 40000 ? '--dbmem' : '' + def database_arg = eggnog_db ? "--database $eggnog_db" : '' + def dmnd_db_arg = eggnog_diamond_db ? "--dmnd_db $eggnog_diamond_db" : '' + """ + if [ "$is_compressed" == "true" ]; then + gzip -c -d $fasta > $fasta_name + fi + + emapper.py \\ + --cpu ${task.cpus} \\ + -i ${fasta_name} \\ + --data_dir ${eggnog_data_dir} \\ + -m diamond \\ + $dmnd_db_arg \\ + $database_arg \\ + --output ${prefix} \\ + ${dbmem} \\ + $args + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + eggnog-mapper: \$(echo \$(emapper.py --version) | grep -o "emapper-[0-9]\\+\\.[0-9]\\+\\.[0-9]\\+" | sed "s/emapper-//") + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.emapper.annotations + touch ${prefix}.emapper.seed_orthologs + touch ${prefix}.emapper.hits + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + eggnog-mapper: \$(echo \$(emapper.py --version) | grep -o "emapper-[0-9]\\+\\.[0-9]\\+\\.[0-9]\\+" | sed "s/emapper-//") + END_VERSIONS + """ +} diff --git a/modules/nf-core/eggnogmapper/meta.yml b/modules/nf-core/eggnogmapper/meta.yml new file mode 100644 index 0000000..76d8aa2 --- /dev/null +++ b/modules/nf-core/eggnogmapper/meta.yml @@ -0,0 +1,62 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/yaml-schema.json +name: "eggnogmapper" +description: Fast genome-wide functional annotation through orthology assignment. +keywords: + - annotation + - orthology + - genomics +tools: + - "eggnogmapper": + description: "Fast genome-wide functional annotation through orthology assignment." + homepage: "https://github.com/eggnogdb/eggnog-mapper" + documentation: "https://github.com/eggnogdb/eggnog-mapper/wiki" + tool_dev_url: "https://github.com/eggnogdb/eggnog-mapper" + doi: "10.1093/molbev/msab293" + licence: ["AGPL v3"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - fasta: + type: file + description: Database of sequences in FASTA format + pattern: "*.{fasta,fa,fasta.gz,fa.gz}" + - eggnog_db: + type: file + description: The eggnog database file (e.g. eggnog-mapper/data/eggnog.db) + pattern: "*.db" + - eggnog_data_dir: + type: directory + description: Directory containing eggnog database files (e.g. eggnog-mapper/data) + pattern: "*" + - eggnog_diamond_db: + type: file + description: The eggnog Diamond protein database file (e.g. eggnog-mapper/data/eggnog_proteins.dmnd) + pattern: "*.dmnd" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'test', single_end:false ]` + - annotations: + type: file + description: TSV with the results from the annotation phase + pattern: "*.emapper.annotations" + - orthologs: + type: file + description: TSV with the results from parsing the hits, linking queries with seed orthologs (with commented metadata) + pattern: "*.emapper.seed_orthologs" + - hits: + type: file + description: TSV with the results from the Diamond search phase + pattern: "*.emapper.hits" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@vagkaratzas" diff --git a/modules/nf-core/eggnogmapper/tests/main.nf.test b/modules/nf-core/eggnogmapper/tests/main.nf.test new file mode 100644 index 0000000..fb707ea --- /dev/null +++ b/modules/nf-core/eggnogmapper/tests/main.nf.test @@ -0,0 +1,56 @@ +nextflow_process { + + name "Test Process EGGNOGMAPPER" + script "../main.nf" + process "EGGNOGMAPPER" + tag "modules" + tag "modules_nfcore" + tag "eggnogmapper" + tag "diamond/makedb" + + test("Should search for protein annotations against the eggnogmapper db") { + + setup { + run("DIAMOND_MAKEDB") { + script "../../diamond/makedb/main.nf" + process { + """ + input[0] = [ [id:'test2'], file(params.test_data['sarscov2']['genome']['proteome_fasta'], checkIfExists: true) ] + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + } + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ [id:'test'], file(params.test_data['sarscov2']['genome']['proteome_fasta'], checkIfExists: true) ] + eggnog_db = file("https://github.com/nf-core/test-datasets/raw/eddf5b0e3336e0f93c81d4b4843b07257f9efaec/data/delete_me/eggnogmapper/eggnog.db", checkIfExists: true) + eggnog_db.copyTo("${workDir}/tmp/eggnog.db") + eggnog_data_dir = "${workDir}/tmp/" + input[1] = eggnog_db + input[2] = eggnog_data_dir + input[3] = DIAMOND_MAKEDB.out.db + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert path(process.out.annotations.get(0).get(1)).readLines().contains("ENSSASP00005000002.1\tENSSASP00005000002.1\t0.0\t14179.0\tCOG0498@1|root,COG0498@2|Bacteria,1MUWQ@1224|Proteobacteria,2VHR6@28216|Betaproteobacteria,2KUMA@206389|Rhodocyclales\t1224|Proteobacteria\tE\tthreonine synthase\t-\t-\t-\t-\t-\t-\t-\t-\t-\t-\t-\t-\t-") }, + { assert path(process.out.orthologs.get(0).get(1)).readLines().contains("ENSSASP00005000002.1\tENSSASP00005000002.1\t0.0\t14179.0\t1\t7096\t1\t7096\t100.0\t100.0\t100.0") }, + { assert snapshot(process.out.hits).match("hits") }, + { assert process.out.versions } + ) + } + + } + +} diff --git a/modules/nf-core/eggnogmapper/tests/main.nf.test.snap b/modules/nf-core/eggnogmapper/tests/main.nf.test.snap new file mode 100644 index 0000000..4e1c837 --- /dev/null +++ b/modules/nf-core/eggnogmapper/tests/main.nf.test.snap @@ -0,0 +1,15 @@ +{ + "hits": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.emapper.hits:md5,864b7a1f902893d8aee6621baeab7be8" + ] + ] + ], + "timestamp": "2023-11-08T20:43:50.173213923" + } +} \ No newline at end of file diff --git a/modules/nf-core/eggnogmapper/tests/tags.yml b/modules/nf-core/eggnogmapper/tests/tags.yml new file mode 100644 index 0000000..284ba2e --- /dev/null +++ b/modules/nf-core/eggnogmapper/tests/tags.yml @@ -0,0 +1,2 @@ +eggnogmapper: + - modules/nf-core/eggnogmapper/** diff --git a/modules/nf-core/gffread/environment.yml b/modules/nf-core/gffread/environment.yml index 5398f71..c6df58a 100644 --- a/modules/nf-core/gffread/environment.yml +++ b/modules/nf-core/gffread/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::gffread=0.12.1 + - bioconda::gffread=0.12.7 diff --git a/modules/nf-core/gffread/main.nf b/modules/nf-core/gffread/main.nf index d8a473e..cfd3e2f 100644 --- a/modules/nf-core/gffread/main.nf +++ b/modules/nf-core/gffread/main.nf @@ -1,32 +1,59 @@ process GFFREAD { - tag "$gff" + tag "$meta.id" label 'process_low' conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/gffread:0.12.1--h8b12597_0' : - 'biocontainers/gffread:0.12.1--h8b12597_0' }" + 'https://depot.galaxyproject.org/singularity/gffread:0.12.7--hdcf5f25_4' : + 'biocontainers/gffread:0.12.7--hdcf5f25_4' }" input: - path gff + tuple val(meta), path(gff) + path fasta output: - path "*.gtf" , emit: gtf , optional: true - path "*.gff3" , emit: gffread_gff , optional: true - path "versions.yml" , emit: versions + tuple val(meta), path("*.gtf") , emit: gtf , optional: true + tuple val(meta), path("*.gff3") , emit: gffread_gff , optional: true + tuple val(meta), path("*.fasta"), emit: gffread_fasta , optional: true + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: - def args = task.ext.args ?: '' - def prefix = task.ext.prefix ?: "${gff.baseName}" - def extension = args.contains("-T") ? 'gtf' : 'gffread.gff3' + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def extension = args.contains("-T") ? 'gtf' : ( ( ['-w', '-x', '-y' ].any { args.contains(it) } ) ? 'fasta' : 'gff3' ) + def fasta_arg = fasta ? "-g $fasta" : '' + def output_name = "${prefix}.${extension}" + def output = extension == "fasta" ? "$output_name" : "-o $output_name" + def args_sorted = args.replaceAll(/(.*)(-[wxy])(.*)/) { all, pre, param, post -> "$pre $post $param" }.trim() + if ( "$output_name" in [ "$gff", "$fasta" ] ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" """ gffread \\ $gff \\ - $args \\ - -o ${prefix}.${extension} + $fasta_arg \\ + $args_sorted \\ + $output + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gffread: \$(gffread --version 2>&1) + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def extension = args.contains("-T") ? 'gtf' : ( ( ['-w', '-x', '-y' ].any { args.contains(it) } ) ? 'fasta' : 'gff3' ) + def fasta_arg = fasta ? "-g $fasta" : '' + def output_name = "${prefix}.${extension}" + def output = extension == "fasta" ? "$output_name" : "-o $output_name" + def args_sorted = args.replaceAll(/(.*)(-[wxy])(.*)/) { all, pre, param, post -> "$pre $post $param" }.trim() + if ( "$output_name" in [ "$gff", "$fasta" ] ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + touch $output_name + cat <<-END_VERSIONS > versions.yml "${task.process}": gffread: \$(gffread --version 2>&1) diff --git a/modules/nf-core/gffread/meta.yml b/modules/nf-core/gffread/meta.yml index d38cbcd..c060282 100644 --- a/modules/nf-core/gffread/meta.yml +++ b/modules/nf-core/gffread/meta.yml @@ -13,11 +13,25 @@ tools: doi: 10.12688/f1000research.23297.1 licence: ["MIT"] input: + - meta: + type: map + description: | + Groovy Map containing meta data + e.g. [ id:'test' ] - gff: type: file description: A reference file in either the GFF3, GFF2 or GTF format. pattern: "*.{gff, gtf}" + - fasta: + type: file + description: A multi-fasta file with the genomic sequences + pattern: "*.{fasta,fa,faa,fas,fsa}" output: + - meta: + type: map + description: | + Groovy Map containing meta data + e.g. [ id:'test' ] - gtf: type: file description: GTF file resulting from the conversion of the GFF input file if '-T' argument is present @@ -25,7 +39,11 @@ output: - gffread_gff: type: file description: GFF3 file resulting from the conversion of the GFF input file if '-T' argument is absent - pattern: "*.{gff3}" + pattern: "*.gff3" + - gffread_fasta: + type: file + description: Fasta file produced when either of '-w', '-x', '-y' parameters is present + pattern: "*.fasta" - versions: type: file description: File containing software versions @@ -34,3 +52,4 @@ authors: - "@edmundmiller" maintainers: - "@edmundmiller" + - "@gallvp" diff --git a/modules/nf-core/gffread/tests/main.nf.test b/modules/nf-core/gffread/tests/main.nf.test index 452aba1..4cd13dc 100644 --- a/modules/nf-core/gffread/tests/main.nf.test +++ b/modules/nf-core/gffread/tests/main.nf.test @@ -18,7 +18,11 @@ nextflow_process { } process { """ - input[0] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) + input[0] = [ + [id: 'test'], + file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) + ] + input[1] = [] """ } } @@ -26,11 +30,40 @@ nextflow_process { then { assertAll ( { assert process.success }, - { assert snapshot( - process.out.gtf, - process.out.versions - ).match() }, - { assert process.out.gffread_gff == [] } + { assert snapshot(process.out).match() }, + { assert process.out.gffread_gff == [] }, + { assert process.out.gffread_fasta == [] } + ) + } + + } + + test("sarscov2-gff3-gtf-stub") { + + options '-stub' + config "./nextflow.config" + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [id: 'test'], + file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) + ] + input[1] = [] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.gffread_gff == [] }, + { assert process.out.gffread_fasta == [] } ) } @@ -46,7 +79,42 @@ nextflow_process { } process { """ - input[0] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) + input[0] = [ + [id: 'test'], + file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) + ] + input[1] = [] + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.gtf == [] }, + { assert process.out.gffread_fasta == [] } + ) + } + + } + + test("sarscov2-gff3-gff3-stub") { + + options '-stub' + config "./nextflow-gff3.config" + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [id: 'test'], + file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) + ] + input[1] = [] """ } } @@ -54,11 +122,99 @@ nextflow_process { then { assertAll ( { assert process.success }, - { assert snapshot( - process.out.gffread_gff, - process.out.versions - ).match() }, + { assert snapshot(process.out).match() }, { assert process.out.gtf == [] }, + { assert process.out.gffread_fasta == [] } + ) + } + + } + + test("sarscov2-gff3-fasta") { + + config "./nextflow-fasta.config" + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [id: 'test'], + file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) + ] + input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.gtf == [] }, + { assert process.out.gffread_gff == [] } + ) + } + + } + + test("sarscov2-gff3-fasta-stub") { + + options '-stub' + config "./nextflow-fasta.config" + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [id: 'test'], + file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) + ] + input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out).match() }, + { assert process.out.gtf == [] }, + { assert process.out.gffread_gff == [] } + ) + } + + } + + test("sarscov2-gff3-fasta-fail-catch") { + + options '-stub' + config "./nextflow-fasta.config" + + when { + params { + outdir = "$outputDir" + } + process { + """ + input[0] = [ + [id: 'genome'], + file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) + ] + input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) + """ + } + } + + then { + assertAll ( + { assert ! process.success }, + { assert process.stdout.toString().contains("Input and output names are the same") } ) } diff --git a/modules/nf-core/gffread/tests/main.nf.test.snap b/modules/nf-core/gffread/tests/main.nf.test.snap index 00a11a4..1526232 100644 --- a/modules/nf-core/gffread/tests/main.nf.test.snap +++ b/modules/nf-core/gffread/tests/main.nf.test.snap @@ -1,24 +1,272 @@ { "sarscov2-gff3-gtf": { "content": [ - [ - "genome.gtf:md5,2394072d7d31530dfd590c4a117bf6e3" - ], - [ - "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" - ] + { + "0": [ + [ + { + "id": "test" + }, + "test.gtf:md5,1ea0ae98d3388e0576407dc4a24ef428" + ] + ], + "1": [ + + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" + ], + "gffread_fasta": [ + + ], + "gffread_gff": [ + + ], + "gtf": [ + [ + { + "id": "test" + }, + "test.gtf:md5,1ea0ae98d3388e0576407dc4a24ef428" + ] + ], + "versions": [ + "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" + ] + } ], - "timestamp": "2024-01-23T20:00:32.688779117" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-09T10:48:56.496187" }, "sarscov2-gff3-gff3": { "content": [ - [ - "genome.gffread.gff3:md5,a7d40d99dcddac23ac673c473279ea2d" - ], - [ - "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" - ] + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.gff3:md5,c4e5da6267c6bee5899a2c204ae1ad91" + ] + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" + ], + "gffread_fasta": [ + + ], + "gffread_gff": [ + [ + { + "id": "test" + }, + "test.gff3:md5,c4e5da6267c6bee5899a2c204ae1ad91" + ] + ], + "gtf": [ + + ], + "versions": [ + "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" + ] + } ], - "timestamp": "2024-01-23T20:07:11.457356625" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-09T10:49:00.892782" + }, + "sarscov2-gff3-gtf-stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" + ], + "gffread_fasta": [ + + ], + "gffread_gff": [ + + ], + "gtf": [ + [ + { + "id": "test" + }, + "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-09T11:11:26.975666" + }, + "sarscov2-gff3-fasta-stub": { + "content": [ + { + "0": [ + + ], + "1": [ + + ], + "2": [ + [ + { + "id": "test" + }, + "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "3": [ + "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" + ], + "gffread_fasta": [ + [ + { + "id": "test" + }, + "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "gffread_gff": [ + + ], + "gtf": [ + + ], + "versions": [ + "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-09T11:11:44.34792" + }, + "sarscov2-gff3-gff3-stub": { + "content": [ + { + "0": [ + + ], + "1": [ + [ + { + "id": "test" + }, + "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + + ], + "3": [ + "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" + ], + "gffread_fasta": [ + + ], + "gffread_gff": [ + [ + { + "id": "test" + }, + "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "gtf": [ + + ], + "versions": [ + "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-09T11:11:35.221671" + }, + "sarscov2-gff3-fasta": { + "content": [ + { + "0": [ + + ], + "1": [ + + ], + "2": [ + [ + { + "id": "test" + }, + "test.fasta:md5,5f8108fb51739a0588ccf0a251de919a" + ] + ], + "3": [ + "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" + ], + "gffread_fasta": [ + [ + { + "id": "test" + }, + "test.fasta:md5,5f8108fb51739a0588ccf0a251de919a" + ] + ], + "gffread_gff": [ + + ], + "gtf": [ + + ], + "versions": [ + "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-09T10:54:02.88143" } } \ No newline at end of file diff --git a/modules/nf-core/gffread/tests/nextflow-fasta.config b/modules/nf-core/gffread/tests/nextflow-fasta.config new file mode 100644 index 0000000..ac6cb14 --- /dev/null +++ b/modules/nf-core/gffread/tests/nextflow-fasta.config @@ -0,0 +1,5 @@ +process { + withName: GFFREAD { + ext.args = '-w -S' + } +} diff --git a/nextflow.config b/nextflow.config index 5f388ff..a5d710a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -34,6 +34,9 @@ params { braker_extra_args = "" liftoff_coverage = 0.9 liftoff_identity = 0.9 + eggnogmapper_db_dir = null + eggnogmapper_tax_scope = null + // Max job request options max_cpus = 12 diff --git a/nextflow_schema.json b/nextflow_schema.json index 3b19410..002acd7 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -178,6 +178,16 @@ "type": "number", "default": 0.9, "description": "Liftoff identity parameter" + }, + "eggnogmapper_db_dir": { + "type": "string", + "description": "Eggnogmapper database directory", + "format": "directory-path" + }, + "eggnogmapper_tax_scope": { + "type": "integer", + "description": "Eggnogmapper taxonomy scopre", + "minimum": 0 } } }, diff --git a/subworkflows/local/fasta_liftoff.nf b/subworkflows/local/fasta_liftoff.nf index 8722a37..e1933e8 100644 --- a/subworkflows/local/fasta_liftoff.nf +++ b/subworkflows/local/fasta_liftoff.nf @@ -46,19 +46,9 @@ workflow FASTA_LIFTOFF { ch_versions = ch_versions.mix(GUNZIP_GFF.out.versions.first()) // MODULE: GFFREAD as GFFREAD_BEFORE_LIFTOFF - ch_gffread_inputs = ch_xref_gunzip_gff - | map { meta, gff -> - [ gff.baseName, meta, gff ] - } // For meta insertion later, remove when GFFREAD has meta - - GFFREAD_BEFORE_LIFTOFF ( ch_gffread_inputs.map { name, meta, gff -> gff } ) + GFFREAD_BEFORE_LIFTOFF ( ch_xref_gunzip_gff, [] ) ch_gffread_gff = GFFREAD_BEFORE_LIFTOFF.out.gffread_gff - | map { gff -> [ gff.baseName - '.gffread', gff ] } - | join(ch_gffread_inputs) - | map { fid, gffread_gff, meta, gff -> [ meta, gffread_gff ] } - // meta insertion - ch_versions = ch_versions.mix(GFFREAD_BEFORE_LIFTOFF.out.versions.first()) // MODULE: LIFTOFF diff --git a/subworkflows/local/gff_eggnogmapper.nf b/subworkflows/local/gff_eggnogmapper.nf new file mode 100644 index 0000000..59c5e3e --- /dev/null +++ b/subworkflows/local/gff_eggnogmapper.nf @@ -0,0 +1,42 @@ +include { GFFREAD as GFF2FASTA_FOR_EGGNOGMAPPER } from '../../modules/nf-core/gffread/main' +include { EGGNOGMAPPER } from '../../modules/nf-core/eggnogmapper/main' + +workflow GFF_EGGNOGMAPPER { + take: + ch_gff // Channel: [ meta, gff ] + ch_fasta // Channel: [ meta, fasta ] + db_folder // val(db_folder) + + main: + + // Versions + ch_versions = Channel.empty() + + // MODULE: GFFREAD as GFF2FASTA_FOR_EGGNOGMAPPER + ch_gffread_inputs = ch_gff + | join(ch_fasta) + + GFF2FASTA_FOR_EGGNOGMAPPER( + ch_gffread_inputs.map { meta, gff, fasta -> [ meta, gff ] }, + ch_gffread_inputs.map { meta, gff, fasta -> fasta } + ) + + ch_gffread_fasta = GFF2FASTA_FOR_EGGNOGMAPPER.out.gffread_fasta + ch_versions = ch_versions.mix(GFF2FASTA_FOR_EGGNOGMAPPER.out.versions.first()) + + + ch_eggnogmapper_inputs = ch_gffread_fasta + | combine(db_folder ? Channel.fromPath(db_folder) : []) + + EGGNOGMAPPER( + ch_eggnogmapper_inputs.map { meta, fasta, db -> [ meta, fasta ] }, + [], + ch_eggnogmapper_inputs.map { meta, fasta, db -> db }, + [ [], [] ] + ) + + ch_versions = ch_versions.mix(EGGNOGMAPPER.out.versions.first()) + + emit: + versions = ch_versions +} diff --git a/subworkflows/local/merge_annotations.nf b/subworkflows/local/merge_annotations.nf index 55c6175..5ba35e3 100644 --- a/subworkflows/local/merge_annotations.nf +++ b/subworkflows/local/merge_annotations.nf @@ -114,6 +114,7 @@ workflow MERGE_ANNOTATIONS { ch_versions = ch_versions.mix(MERGE_BRAKER_LIFTOFF.out.versions.first()) emit: - merged_gff = ch_merged_gff - versions = ch_versions + braker_purged = ch_braker_purged // [ meta, gff3 ] + merged_gff = ch_merged_gff // [ meta, gff3 ] + versions = ch_versions // [ versions.yml ] } diff --git a/workflows/pangene.nf b/workflows/pangene.nf index bffaf3a..65d52d8 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -7,6 +7,7 @@ include { PREPARE_EXT_PROTS } from '../subworkflows/local/pr include { FASTA_BRAKER3 } from '../subworkflows/local/fasta_braker3' include { FASTA_LIFTOFF } from '../subworkflows/local/fasta_liftoff' include { MERGE_ANNOTATIONS } from '../subworkflows/local/merge_annotations' +include { GFF_EGGNOGMAPPER } from '../subworkflows/local/gff_eggnogmapper' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions' log.info paramsSummaryLog(workflow) @@ -201,8 +202,18 @@ workflow PANGENE { MERGE_ANNOTATIONS ( ch_braker_gff3, ch_liftoff_gff3 ) ch_merged_gff = MERGE_ANNOTATIONS.out.merged_gff + ch_braker_purged = MERGE_ANNOTATIONS.out.braker_purged ch_versions = ch_versions.mix(MERGE_ANNOTATIONS.out.versions) + // SUBWORKFLOW: GFF_EGGNOGMAPPER + GFF_EGGNOGMAPPER( + ch_braker_purged, + ch_valid_target_assembly, + params.eggnogmapper_db_dir, + ) + + ch_versions = ch_versions.mix(GFF_EGGNOGMAPPER.out.versions) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From 662c4b1a6fab81a137db892d0554ef5a4db1d857 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 12 Apr 2024 13:06:26 +1200 Subject: [PATCH 19/40] Now using TSEBRA to purge models which do not have full intron support from BRAKER hints --- CHANGELOG.md | 5 +- assets/schema_input.json | 10 +- assets/tsebra-1form.cfg | 16 ++ assets/tsebra-default.cfg | 16 ++ conf/modules.config | 16 +- modules.json | 15 ++ .../agat/convertspgff2gtf/environment.yml | 7 + modules/nf-core/agat/convertspgff2gtf/main.nf | 48 +++++ .../nf-core/agat/convertspgff2gtf/meta.yml | 43 ++++ .../agat/convertspgff2gtf/tests/main.nf.test | 62 ++++++ .../convertspgff2gtf/tests/main.nf.test.snap | 28 +++ .../agat/convertspgff2gtf/tests/tags.yml | 2 + .../agat/convertspgxf2gxf/environment.yml | 7 + modules/nf-core/agat/convertspgxf2gxf/main.nf | 48 +++++ .../nf-core/agat/convertspgxf2gxf/meta.yml | 43 ++++ .../agat/convertspgxf2gxf/tests/main.nf.test | 60 ++++++ .../convertspgxf2gxf/tests/main.nf.test.snap | 71 +++++++ .../agat/convertspgxf2gxf/tests/tags.yml | 2 + modules/pfr/tsebra/environment.yml | 9 + modules/pfr/tsebra/main.nf | 61 ++++++ modules/pfr/tsebra/meta.yml | 66 +++++++ modules/pfr/tsebra/tests/main.nf.test | 91 +++++++++ modules/pfr/tsebra/tests/main.nf.test.snap | 100 ++++++++++ modules/pfr/tsebra/tests/tags.yml | 2 + nextflow.config | 1 + nextflow_schema.json | 5 + subworkflows/local/fasta_braker3.nf | 2 +- subworkflows/local/merge_annotations.nf | 120 ------------ subworkflows/local/purge_breaker_models.nf | 184 ++++++++++++++++++ workflows/pangene.nf | 38 ++-- 30 files changed, 1024 insertions(+), 154 deletions(-) create mode 100644 assets/tsebra-1form.cfg create mode 100644 assets/tsebra-default.cfg create mode 100644 modules/nf-core/agat/convertspgff2gtf/environment.yml create mode 100644 modules/nf-core/agat/convertspgff2gtf/main.nf create mode 100644 modules/nf-core/agat/convertspgff2gtf/meta.yml create mode 100644 modules/nf-core/agat/convertspgff2gtf/tests/main.nf.test create mode 100644 modules/nf-core/agat/convertspgff2gtf/tests/main.nf.test.snap create mode 100644 modules/nf-core/agat/convertspgff2gtf/tests/tags.yml create mode 100644 modules/nf-core/agat/convertspgxf2gxf/environment.yml create mode 100644 modules/nf-core/agat/convertspgxf2gxf/main.nf create mode 100644 modules/nf-core/agat/convertspgxf2gxf/meta.yml create mode 100644 modules/nf-core/agat/convertspgxf2gxf/tests/main.nf.test create mode 100644 modules/nf-core/agat/convertspgxf2gxf/tests/main.nf.test.snap create mode 100644 modules/nf-core/agat/convertspgxf2gxf/tests/tags.yml create mode 100644 modules/pfr/tsebra/environment.yml create mode 100644 modules/pfr/tsebra/main.nf create mode 100644 modules/pfr/tsebra/meta.yml create mode 100644 modules/pfr/tsebra/tests/main.nf.test create mode 100644 modules/pfr/tsebra/tests/main.nf.test.snap create mode 100644 modules/pfr/tsebra/tests/tags.yml delete mode 100644 subworkflows/local/merge_annotations.nf create mode 100644 subworkflows/local/purge_breaker_models.nf diff --git a/CHANGELOG.md b/CHANGELOG.md index 0670fac..24e46c4 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 0.2.0+dev - [09-April-2024] +## 0.2.0+dev - [12-April-2024] ### `Added` @@ -29,9 +29,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 20. Now using a txt file for `params.external_protein_fastas` 21. Now using nf-validation for `params.liftoff_annotations` 22. Now using nf-validation for all the parameters -23. Added `MERGE_ANNOTATIONS` sub-workflow +23. Added `PURGE_BREAKER_MODELS` sub-workflow 24. Added `GFF_EGGNOGMAPPER` sub-workflow 25. Now using a custom version of `GFFREAD` which supports `meta` and `fasta` +26. Now using TSEBRA to purge models which do not have full intron support from BRAKER hints ### `Fixed` diff --git a/assets/schema_input.json b/assets/schema_input.json index da31bc3..d8e0fdd 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -36,7 +36,7 @@ ], "errorMessage": "TE library FASTA file path cannot contain spaces and must have extension '.f(a|asta|as|sa|na)' or '.f(a|asta|as|sa|na).gz'" }, - "annotation_gff3": { + "braker_gff3": { "anyOf": [ { "type": "string", @@ -47,10 +47,10 @@ "maxLength": 0 } ], - "errorMessage": "Annotation GFF3 file path cannot contain spaces and must have extension '.gff.gz', '.gff3.gz', '.gff' or '.gff3'", - "dependentRequired": ["annotation_hints"] + "errorMessage": "BRAKER GFF3 file path cannot contain spaces and must have extension '.gff.gz', '.gff3.gz', '.gff' or '.gff3'", + "dependentRequired": ["braker_hints"] }, - "annotation_hints": { + "braker_hints": { "anyOf": [ { "type": "string", @@ -61,7 +61,7 @@ "maxLength": 0 } ], - "errorMessage": "Annotation hints GFF/GFF3 file path cannot contain spaces and must have extension '.gff.gz', '.gff3.gz', '.gff' or '.gff3'" + "errorMessage": "BRAKER hints GFF/GFF3 file path cannot contain spaces and must have extension '.gff.gz', '.gff3.gz', '.gff' or '.gff3'" } }, "required": ["tag", "fasta", "is_masked"] diff --git a/assets/tsebra-1form.cfg b/assets/tsebra-1form.cfg new file mode 100644 index 0000000..2b076fa --- /dev/null +++ b/assets/tsebra-1form.cfg @@ -0,0 +1,16 @@ +# Weight for each hint source +# Values have to be >= 0 +P 1 +E 20 +C 1 +M 1 +# Required fraction of supported introns or supported start/stop-codons for a transcript +# Values have to be in [0,1] +intron_support 1.0 +stasto_support 2 +# Allowed difference for each feature +# Values have to be in [0,1] +e_1 0 +e_2 0 +e_3 0 +e_4 0 diff --git a/assets/tsebra-default.cfg b/assets/tsebra-default.cfg new file mode 100644 index 0000000..8ec1a98 --- /dev/null +++ b/assets/tsebra-default.cfg @@ -0,0 +1,16 @@ +# Weight for each hint source +# Values have to be >= 0 +P 1 +E 20 +C 1 +M 1 +# Required fraction of supported introns or supported start/stop-codons for a transcript +# Values have to be in [0,1] +intron_support 1.0 +stasto_support 2 +# Allowed difference for each feature +# Values have to be in [0,1] +e_1 0.1 +e_2 0.5 +e_3 0.05 +e_4 0.2 diff --git a/conf/modules.config b/conf/modules.config index fe3d2f8..b00c35a 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -177,19 +177,13 @@ if(params.liftoff_annotations) { } process { - withName: 'VALIDATE_PURGING_BY_AGAT' { - ext.prefix = { "${meta.id}_post_purge" } - } - - withName: 'MERGE_BRAKER_LIFTOFF' { - ext.prefix = { "${meta.id}.braker.liftoff.merged" } + withName: 'AGAT_CONVERTSPGFF2GTF' { + ext.args = '--gtf_version relax' + } - publishDir = [ - path: { "${params.outdir}/gff" }, - mode: "copy", - saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, - ] + withName: 'AGAT_SPFILTERFEATUREFROMKILLLIST' { + ext.prefix = { "${meta.id}.purged" } } } diff --git a/modules.json b/modules.json index 57cbcfc..da86263 100644 --- a/modules.json +++ b/modules.json @@ -44,6 +44,11 @@ "branch": "main", "git_sha": "9da0567f685b2772f65290f2bd6d6347671c8310", "installed_by": ["modules"] + }, + "tsebra": { + "branch": "main", + "git_sha": "a3b86c357980e5244cb313027c1d980d89c19ef4", + "installed_by": ["modules"] } } }, @@ -76,6 +81,16 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { + "agat/convertspgff2gtf": { + "branch": "master", + "git_sha": "15f1cf0a1a12da63839c336eb1ecd96d03320e94", + "installed_by": ["modules"] + }, + "agat/convertspgxf2gxf": { + "branch": "master", + "git_sha": "71ccbccbd498af48c33939e1123517340bab3d6f", + "installed_by": ["modules"] + }, "cat/cat": { "branch": "master", "git_sha": "9437e6053dccf4aafa022bfd6e7e9de67e625af8", diff --git a/modules/nf-core/agat/convertspgff2gtf/environment.yml b/modules/nf-core/agat/convertspgff2gtf/environment.yml new file mode 100644 index 0000000..381154f --- /dev/null +++ b/modules/nf-core/agat/convertspgff2gtf/environment.yml @@ -0,0 +1,7 @@ +name: agat_convertspgff2gtf +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::agat=1.0.0 diff --git a/modules/nf-core/agat/convertspgff2gtf/main.nf b/modules/nf-core/agat/convertspgff2gtf/main.nf new file mode 100644 index 0000000..8f1f8b4 --- /dev/null +++ b/modules/nf-core/agat/convertspgff2gtf/main.nf @@ -0,0 +1,48 @@ +process AGAT_CONVERTSPGFF2GTF { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/agat:1.0.0--pl5321hdfd78af_0' : + 'biocontainers/agat:1.0.0--pl5321hdfd78af_0' }" + + input: + tuple val(meta), path(gff) + + output: + tuple val(meta), path("*.agat.gtf"), emit: output_gtf + tuple val(meta), path("*.log"), emit: log + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + agat_convert_sp_gff2gtf.pl \\ + --gff $gff \\ + --output ${prefix}.agat.gtf \\ + $args + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + agat: \$(agat_convert_sp_gff2gtf.pl --help | sed '4!d; s/.*v//') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.agat.gtf + touch ${gff}.agat.log + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + agat: \$(agat_convert_sp_gff2gtf.pl --help | sed '4!d; s/.*v//') + END_VERSIONS + """ +} diff --git a/modules/nf-core/agat/convertspgff2gtf/meta.yml b/modules/nf-core/agat/convertspgff2gtf/meta.yml new file mode 100644 index 0000000..dcdc8d9 --- /dev/null +++ b/modules/nf-core/agat/convertspgff2gtf/meta.yml @@ -0,0 +1,43 @@ +name: agat_convertspgff2gtf +description: | + Converts a GFF/GTF file into a proper GTF file +keywords: + - genome + - gff + - gtf + - conversion +tools: + - agat: + description: "AGAT is a toolkit for manipulation and getting information from GFF/GTF files" + homepage: "https://github.com/NBISweden/AGAT" + documentation: "https://agat.readthedocs.io/" + tool_dev_url: "https://github.com/NBISweden/AGAT" + doi: "10.5281/zenodo.3552717" + licence: ["GPL v3"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - gff: + type: file + description: Annotation file in GFF3/GTF format + pattern: "*.{gff, gtf}" +output: + - output_gtf: + type: file + description: Annotation file in GTF format + pattern: "*.{gtf}" + - log: + type: file + description: Log file of the conversion process + pattern: "*.{log}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@toniher" +maintainers: + - "@toniher" diff --git a/modules/nf-core/agat/convertspgff2gtf/tests/main.nf.test b/modules/nf-core/agat/convertspgff2gtf/tests/main.nf.test new file mode 100644 index 0000000..9accfec --- /dev/null +++ b/modules/nf-core/agat/convertspgff2gtf/tests/main.nf.test @@ -0,0 +1,62 @@ +nextflow_process { + + name "Test Process AGAT_CONVERTSPGFF2GTF" + script "../main.nf" + process "AGAT_CONVERTSPGFF2GTF" + + tag "modules" + tag "modules_nfcore" + tag "agat" + tag "agat/convertspgff2gtf" + + test("sarscov2 - genome [gff3]") { + + when { + process { + """ + input[0] = [ + [ id: 'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.output_gtf, + process.out.versions).match() }, + { assert path(process.out.log[0][1]).exists() } + ) + } + + } + + test("sarscov2 - genome [gff3] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id: 'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gff3'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.output_gtf.collect { file(it[1]).getName() } + + process.out.log.collect { file(it[1]).getName() } + + process.out.versions ).match() } + ) + } + + } + +} diff --git a/modules/nf-core/agat/convertspgff2gtf/tests/main.nf.test.snap b/modules/nf-core/agat/convertspgff2gtf/tests/main.nf.test.snap new file mode 100644 index 0000000..6193be8 --- /dev/null +++ b/modules/nf-core/agat/convertspgff2gtf/tests/main.nf.test.snap @@ -0,0 +1,28 @@ +{ + "sarscov2 - genome [gff3] - stub": { + "content": [ + [ + "test.agat.gtf", + "genome.gff3.agat.log", + "versions.yml:md5,dcbde1b24eb36571645f2d4bd4b4e551" + ] + ], + "timestamp": "2023-12-24T23:36:49.538312808" + }, + "sarscov2 - genome [gff3]": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.agat.gtf:md5,bbe333239767d048eb8392bba6856616" + ] + ], + [ + "versions.yml:md5,dcbde1b24eb36571645f2d4bd4b4e551" + ] + ], + "timestamp": "2023-12-24T23:36:39.319717066" + } +} \ No newline at end of file diff --git a/modules/nf-core/agat/convertspgff2gtf/tests/tags.yml b/modules/nf-core/agat/convertspgff2gtf/tests/tags.yml new file mode 100644 index 0000000..7a59648 --- /dev/null +++ b/modules/nf-core/agat/convertspgff2gtf/tests/tags.yml @@ -0,0 +1,2 @@ +agat/convertspgff2gtf: + - "modules/nf-core/agat/convertspgff2gtf/**" diff --git a/modules/nf-core/agat/convertspgxf2gxf/environment.yml b/modules/nf-core/agat/convertspgxf2gxf/environment.yml new file mode 100644 index 0000000..6ed34fa --- /dev/null +++ b/modules/nf-core/agat/convertspgxf2gxf/environment.yml @@ -0,0 +1,7 @@ +name: agat_convertspgxf2gxf +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - bioconda::agat=1.4.0 diff --git a/modules/nf-core/agat/convertspgxf2gxf/main.nf b/modules/nf-core/agat/convertspgxf2gxf/main.nf new file mode 100644 index 0000000..b9a7668 --- /dev/null +++ b/modules/nf-core/agat/convertspgxf2gxf/main.nf @@ -0,0 +1,48 @@ +process AGAT_CONVERTSPGXF2GXF { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/agat:1.4.0--pl5321hdfd78af_0' : + 'biocontainers/agat:1.4.0--pl5321hdfd78af_0' }" + + input: + tuple val(meta), path(gxf) + + output: + tuple val(meta), path("*.agat.gff") , emit: output_gff + tuple val(meta), path("*.log") , emit: log + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + agat_convert_sp_gxf2gxf.pl \\ + --gxf $gxf \\ + --output ${prefix}.agat.gff \\ + $args + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + agat: \$(agat_convert_sp_gxf2gxf.pl --help | sed -n 's/.*(AGAT) - Version: \\(.*\\) .*/\\1/p') + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + """ + touch ${prefix}.agat.gff + touch ${gxf}.agat.log + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + agat: \$(agat_convert_sp_gxf2gxf.pl --help | sed -n 's/.*(AGAT) - Version: \\(.*\\) .*/\\1/p') + END_VERSIONS + """ +} diff --git a/modules/nf-core/agat/convertspgxf2gxf/meta.yml b/modules/nf-core/agat/convertspgxf2gxf/meta.yml new file mode 100644 index 0000000..0ef9881 --- /dev/null +++ b/modules/nf-core/agat/convertspgxf2gxf/meta.yml @@ -0,0 +1,43 @@ +name: agat_convertspgxf2gxf +description: | + Fixes and standardizes GFF/GTF files and outputs a cleaned GFF/GTF file +keywords: + - genome + - gff + - gtf + - conversion +tools: + - agat: + description: "AGAT is a toolkit for manipulation and getting information from GFF/GTF files" + homepage: "https://github.com/NBISweden/AGAT" + documentation: "https://agat.readthedocs.io/" + tool_dev_url: "https://github.com/NBISweden/AGAT" + doi: "10.5281/zenodo.3552717" + licence: ["GPL v3"] +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. [ id:'test', single_end:false ] + - gxf: + type: file + description: Annotation file in GFF3/GTF format + pattern: "*.{gff, gtf}" +output: + - output_gff: + type: file + description: Cleaned annotation file in GFF3 format + pattern: "*.{gff}" + - log: + type: file + description: Log file of the conversion process + pattern: "*.{log}" + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" +authors: + - "@toniher" +maintainers: + - "@toniher" diff --git a/modules/nf-core/agat/convertspgxf2gxf/tests/main.nf.test b/modules/nf-core/agat/convertspgxf2gxf/tests/main.nf.test new file mode 100644 index 0000000..db85991 --- /dev/null +++ b/modules/nf-core/agat/convertspgxf2gxf/tests/main.nf.test @@ -0,0 +1,60 @@ +nextflow_process { + + name "Test Process AGAT_CONVERTSPGXF2GXF" + script "../main.nf" + process "AGAT_CONVERTSPGXF2GXF" + + tag "modules" + tag "modules_nfcore" + tag "agat" + tag "agat/convertspgxf2gxf" + + test("sarscov2 genome [gtf]") { + + when { + process { + """ + input[0] = [ + [ id: 'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gtf'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.output_gff, + process.out.versions).match() }, + { assert path(process.out.log[0][1]).exists() } + ) + } + + } + + test("sarscov2 genome [gtf] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id: 'test' ], // meta map + file(params.test_data['sarscov2']['genome']['genome_gtf'], checkIfExists: true) + ] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/agat/convertspgxf2gxf/tests/main.nf.test.snap b/modules/nf-core/agat/convertspgxf2gxf/tests/main.nf.test.snap new file mode 100644 index 0000000..e89073f --- /dev/null +++ b/modules/nf-core/agat/convertspgxf2gxf/tests/main.nf.test.snap @@ -0,0 +1,71 @@ +{ + "sarscov2 genome [gtf] - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.agat.gff:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "genome.gtf.agat.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,5ec6166c5c080ec4bc08a8fe55ada486" + ], + "log": [ + [ + { + "id": "test" + }, + "genome.gtf.agat.log:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "output_gff": [ + [ + { + "id": "test" + }, + "test.agat.gff:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,5ec6166c5c080ec4bc08a8fe55ada486" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-12T12:25:34.583294" + }, + "sarscov2 genome [gtf]": { + "content": [ + [ + [ + { + "id": "test" + }, + "test.agat.gff:md5,7d7e9bcd82a2f0bb7d8a38f85e82f0bc" + ] + ], + [ + "versions.yml:md5,5ec6166c5c080ec4bc08a8fe55ada486" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-12T12:21:21.310464" + } +} \ No newline at end of file diff --git a/modules/nf-core/agat/convertspgxf2gxf/tests/tags.yml b/modules/nf-core/agat/convertspgxf2gxf/tests/tags.yml new file mode 100644 index 0000000..85c7000 --- /dev/null +++ b/modules/nf-core/agat/convertspgxf2gxf/tests/tags.yml @@ -0,0 +1,2 @@ +agat/convertspgxf2gxf: + - "modules/nf-core/agat/convertspgxf2gxf/**" diff --git a/modules/pfr/tsebra/environment.yml b/modules/pfr/tsebra/environment.yml new file mode 100644 index 0000000..3505512 --- /dev/null +++ b/modules/pfr/tsebra/environment.yml @@ -0,0 +1,9 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/environment-schema.json +name: "tsebra" +channels: + - conda-forge + - bioconda + - defaults +dependencies: + - "bioconda::tsebra=1.1.2.4" diff --git a/modules/pfr/tsebra/main.nf b/modules/pfr/tsebra/main.nf new file mode 100644 index 0000000..c92ade7 --- /dev/null +++ b/modules/pfr/tsebra/main.nf @@ -0,0 +1,61 @@ +process TSEBRA { + tag "$meta.id" + label 'process_single' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/tsebra:1.1.2.4--pyhca03a8a_0': + 'biocontainers/tsebra:1.1.2.4--pyhca03a8a_0' }" + + input: + tuple val(meta), path(gtfs) + path hints_files + path keep_gtfs + path config + + output: + tuple val(meta), path("*.gtf"), emit: tsebra_gtf + tuple val(meta), path("*.tsv"), emit: tsebra_scores + path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def gtf_arg = '-g ' + gtfs.collect { "$it" }.join(',') + def hints_arg = '-e ' + hints_files.collect { "$it" }.join(',') + def keep_arg = keep_gtfs ? ( '-k ' + keep_gtfs.collect { "$it" }.join(',') ) : '' + def config_arg = config ? "-c $config" : '' + def VERSION = '1.1.2.4' + """ + tsebra.py \\ + $gtf_arg \\ + $hints_arg \\ + $keep_arg \\ + $config_arg \\ + $args \\ + -o ${prefix}.gtf \\ + -s ${prefix}.tsv + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tsebra: $VERSION + END_VERSIONS + """ + + stub: + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${meta.id}" + def VERSION = '1.1.2.4' + """ + touch ${prefix}.gtf + touch ${prefix}.tsv + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + tsebra: $VERSION + END_VERSIONS + """ +} diff --git a/modules/pfr/tsebra/meta.yml b/modules/pfr/tsebra/meta.yml new file mode 100644 index 0000000..18660d4 --- /dev/null +++ b/modules/pfr/tsebra/meta.yml @@ -0,0 +1,66 @@ +--- +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json +name: "tsebra" +description: Transcript Selector for BRAKER TSEBRA combines gene predictions by selecing transcripts based on their extrisic evidence support +keywords: + - genomics + - transcript + - selector + - gene + - prediction + - evidence +tools: + - "tsebra": + description: TSEBRA is a combiner tool that selects transcripts from gene predictions based on the support by extrisic evidence in form of introns and start/stop codons + homepage: "https://github.com/Gaius-Augustus/TSEBRA" + documentation: "https://github.com/Gaius-Augustus/TSEBRA" + tool_dev_url: "https://github.com/Gaius-Augustus/TSEBRA" + doi: "10.1186/s12859-021-04482-0" + licence: ["Artistic-2.0"] + +input: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - gtfs: + type: list + description: List of gene prediction files in gtf + pattern: "*.gtf" + - hints_files: + type: list + description: List of files containing extrinsic evidence in gff + pattern: "*.gff" + - keep_gtfs: + type: list + description: | + List of gene prediction files in gtf. These gene sets are used the same way as other inputs, but TSEBRA ensures that all + transcripts from these gene sets are included in the output + pattern: "*.gtf" + - config: + type: file + description: Configuration file that sets the parameter for TSEBRA + pattern: "*.cfg" +output: + - meta: + type: map + description: | + Groovy Map containing sample information + e.g. `[ id:'sample1' ]` + - versions: + type: file + description: File containing software versions + pattern: "versions.yml" + - tsebra_gtf: + type: file + description: Output file for the combined gene predictions in gtf + pattern: "*.gtf" + - tsebra_scores: + type: file + description: Transcript scores as a table + pattern: "*.tsv" +authors: + - "@GallVp" +maintainers: + - "@GallVp" diff --git a/modules/pfr/tsebra/tests/main.nf.test b/modules/pfr/tsebra/tests/main.nf.test new file mode 100644 index 0000000..ddf7a6c --- /dev/null +++ b/modules/pfr/tsebra/tests/main.nf.test @@ -0,0 +1,91 @@ +nextflow_process { + + name "Test Process TSEBRA" + script "../main.nf" + process "TSEBRA" + + tag "modules" + tag "modules_nfcore" + tag "tsebra" + tag "nf-core/gunzip/main" + + test("actinidia_chinensis-genome") { + + setup { + run('GUNZIP', alias: 'GUNZIP_GTF') { + script "../../../nf-core/gunzip/main" + + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['actinidia_chinensis']['genome']['genome_1_gtf_gz'], checkIfExists: true) + ] + """ + } + } + + run('GUNZIP', alias: 'GUNZIP_HINTS') { + script "../../../nf-core/gunzip/main" + + process { + """ + input[0] = [ + [ id:'test' ], // meta map + file(params.test_data['actinidia_chinensis']['genome']['genome_1_hints_gff_gz'], checkIfExists: true) + ] + """ + } + } + } + + when { + process { + """ + input[0] = GUNZIP_GTF.out.gunzip.map { meta, gtf -> [ meta, [ gtf ] ] } + input[1] = GUNZIP_HINTS.out.gunzip.map { meta, gff -> [ gff ] } + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("actinidia_chinensis-genome-stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test' ], // meta map + [ file(params.test_data['actinidia_chinensis']['genome']['genome_1_gtf_gz'], checkIfExists: true) ] + ] + input[1] = [ + file(params.test_data['actinidia_chinensis']['genome']['genome_1_hints_gff_gz'], checkIfExists: true) + ] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/pfr/tsebra/tests/main.nf.test.snap b/modules/pfr/tsebra/tests/main.nf.test.snap new file mode 100644 index 0000000..4d9e15f --- /dev/null +++ b/modules/pfr/tsebra/tests/main.nf.test.snap @@ -0,0 +1,100 @@ +{ + "actinidia_chinensis-genome-stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "2": [ + "versions.yml:md5,6d60045f4f9b66baa508c174ae6a6408" + ], + "tsebra_gtf": [ + [ + { + "id": "test" + }, + "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "tsebra_scores": [ + [ + { + "id": "test" + }, + "test.tsv:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ], + "versions": [ + "versions.yml:md5,6d60045f4f9b66baa508c174ae6a6408" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-11T12:20:49.618044" + }, + "actinidia_chinensis-genome": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + "test.gtf:md5,7c781c919e6aa20561f72dea09474f74" + ] + ], + "1": [ + [ + { + "id": "test" + }, + "test.tsv:md5,ad7bd858c1838b40286609a311f3a891" + ] + ], + "2": [ + "versions.yml:md5,6d60045f4f9b66baa508c174ae6a6408" + ], + "tsebra_gtf": [ + [ + { + "id": "test" + }, + "test.gtf:md5,7c781c919e6aa20561f72dea09474f74" + ] + ], + "tsebra_scores": [ + [ + { + "id": "test" + }, + "test.tsv:md5,ad7bd858c1838b40286609a311f3a891" + ] + ], + "versions": [ + "versions.yml:md5,6d60045f4f9b66baa508c174ae6a6408" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-04-11T12:20:45.666076" + } +} \ No newline at end of file diff --git a/modules/pfr/tsebra/tests/tags.yml b/modules/pfr/tsebra/tests/tags.yml new file mode 100644 index 0000000..7594182 --- /dev/null +++ b/modules/pfr/tsebra/tests/tags.yml @@ -0,0 +1,2 @@ +tsebra: + - "modules/pfr/tsebra/**" diff --git a/nextflow.config b/nextflow.config index a5d710a..c095d2d 100644 --- a/nextflow.config +++ b/nextflow.config @@ -32,6 +32,7 @@ params { // Annotation options braker_extra_args = "" + braker_allow_isoforms = true liftoff_coverage = 0.9 liftoff_identity = 0.9 eggnogmapper_db_dir = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 002acd7..2192cb1 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -167,6 +167,11 @@ "type": "string", "description": "Extra arguments for BRAKER" }, + "braker_allow_isoforms": { + "type": "boolean", + "default": true, + "description": "Allow multiple isoforms for gene models" + }, "liftoff_coverage": { "type": "number", "default": 0.9, diff --git a/subworkflows/local/fasta_braker3.nf b/subworkflows/local/fasta_braker3.nf index 0ae0628..b76bdf7 100644 --- a/subworkflows/local/fasta_braker3.nf +++ b/subworkflows/local/fasta_braker3.nf @@ -55,7 +55,7 @@ workflow FASTA_BRAKER3 { // WORKFLOW: FILE_GUNZIP as BRAKER_HINTS_GUNZIP BRAKER_HINTS_GUNZIP ( ch_braker_hints ) - ch_braker_hints = BRAKER_GFF3_GUNZIP.out.gunzip + ch_braker_hints = BRAKER_HINTS_GUNZIP.out.gunzip ch_versions = ch_versions.mix(BRAKER_HINTS_GUNZIP.out.versions) emit: diff --git a/subworkflows/local/merge_annotations.nf b/subworkflows/local/merge_annotations.nf deleted file mode 100644 index 5ba35e3..0000000 --- a/subworkflows/local/merge_annotations.nf +++ /dev/null @@ -1,120 +0,0 @@ -include { GFFCOMPARE as COMPARE_BRAKER_TO_LIFTOFF } from '../../modules/nf-core/gffcompare/main' -include { AGAT_SPFILTERFEATUREFROMKILLLIST } from '../../modules/pfr/agat/spfilterfeaturefromkilllist/main' -include { GFFCOMPARE as VALIDATE_PURGING_BY_AGAT } from '../../modules/nf-core/gffcompare/main' -include { AGAT_SPMERGEANNOTATIONS as MERGE_BRAKER_LIFTOFF } from '../../modules/pfr/agat/spmergeannotations/main' - -workflow MERGE_ANNOTATIONS { - take: - braker_gff3 // [ meta, gff3 ] - liftoff_gff3 // [ meta, gff3 ] - - main: - ch_versions = Channel.empty() - - // MODULE: GFFCOMPARE as COMPARE_BRAKER_TO_LIFTOFF - ch_comparison_inputs = braker_gff3 - | join(liftoff_gff3) - - - COMPARE_BRAKER_TO_LIFTOFF ( - ch_comparison_inputs.map { meta, braker, liftoff -> [ meta, braker ] }, - [ [], [], [] ], - ch_comparison_inputs.map { meta, braker, liftoff -> [ meta, liftoff ] }, - ) - - ch_tracking = COMPARE_BRAKER_TO_LIFTOFF.out.tracking - ch_versions = ch_versions.mix(COMPARE_BRAKER_TO_LIFTOFF.out.versions.first()) - - // COLLECTFILE: Transcript level kill list - ch_kill_list = ch_tracking - | map { meta, tracking -> - - def kept_lines = tracking.readLines() - .findAll { line -> - def cols = line.split('\t') - - ( cols[3] != 'u' ) && ( cols[3] != 'p' ) - } - - def tx_kill_list = kept_lines - .collect { line -> - def cols = line.split('\t') - - def matched = cols[4] =~ /q1:([^\|]+)\|([^\|]+)/ - - matched[0][2].trim() - } - - [ "${meta.id}.kill.list.txt" ] + tx_kill_list - } - | collectFile(newLine: true) - | map { file -> - [ [ id: file.baseName.replace(".kill.list", "") ], file ] - } - - // MODULE: AGAT_SPFILTERFEATUREFROMKILLLIST - ch_agat_kill_inputs = braker_gff3 - | join(ch_kill_list) - - - AGAT_SPFILTERFEATUREFROMKILLLIST( - ch_agat_kill_inputs.map { meta, gff, kill -> [ meta, gff ] }, - ch_agat_kill_inputs.map { meta, gff, kill -> kill }, - [] // default config - ) - - ch_braker_purged = AGAT_SPFILTERFEATUREFROMKILLLIST.out.gff - ch_versions = ch_versions.mix(AGAT_SPFILTERFEATUREFROMKILLLIST.out.versions.first()) - - // MODULE: GFFCOMPARE as VALIDATE_PURGING_BY_AGAT - ch_validation_inputs = ch_braker_purged - | join(liftoff_gff3) - - VALIDATE_PURGING_BY_AGAT( - ch_validation_inputs.map { meta, braker, liftoff -> [ meta, braker ] }, - [ [], [], [] ], - ch_validation_inputs.map { meta, braker, liftoff -> [ meta, liftoff ] } - ) - - ch_validation_tracking = VALIDATE_PURGING_BY_AGAT.out.tracking - ch_versions = ch_versions.mix(VALIDATE_PURGING_BY_AGAT.out.versions.first()) - - // VALIDATE: All gffcompare codes in [ 'u', 'p' ] - ch_validation_output = ch_validation_tracking - | map { meta, tracking -> - - def tx_list = tracking.readLines() - .collect { line -> - def cols = line.split('\t') - def matched = cols[4] =~ /q1:([^\|]+)\|([^\|]+)/ - def tx_id = matched[0][2].trim() - def code = cols[3] - - if ( code != 'u' && code != 'p' ) { - log.warn "In ${meta.id}, transcript $tx_id has code ${code}." + - " This issue can break the downstream logic in the pipeline." + - " Please contact the devs!" - } - - [ tx_id, code ] - } - - [ meta, tx_list ] - } - - // MODULE: AGAT_SPMERGEANNOTATIONS as MERGE_BRAKER_LIFTOFF - ch_merge_inputs = ch_braker_purged - | join(liftoff_gff3) - MERGE_BRAKER_LIFTOFF( - ch_merge_inputs.map { meta, braker, liftoff -> [ meta, [ braker, liftoff ] ] }, - [] - ) - - ch_merged_gff = MERGE_BRAKER_LIFTOFF.out.gff - ch_versions = ch_versions.mix(MERGE_BRAKER_LIFTOFF.out.versions.first()) - - emit: - braker_purged = ch_braker_purged // [ meta, gff3 ] - merged_gff = ch_merged_gff // [ meta, gff3 ] - versions = ch_versions // [ versions.yml ] -} diff --git a/subworkflows/local/purge_breaker_models.nf b/subworkflows/local/purge_breaker_models.nf new file mode 100644 index 0000000..2f00cf5 --- /dev/null +++ b/subworkflows/local/purge_breaker_models.nf @@ -0,0 +1,184 @@ +include { AGAT_CONVERTSPGFF2GTF } from '../../modules/nf-core/agat/convertspgff2gtf/main' +include { TSEBRA } from '../../modules/pfr/tsebra/main' +include { AGAT_CONVERTSPGXF2GXF } from '../../modules/nf-core/agat/convertspgxf2gxf/main' +include { GFFCOMPARE as COMPARE_BRAKER_TO_LIFTOFF } from '../../modules/nf-core/gffcompare/main' +include { AGAT_SPFILTERFEATUREFROMKILLLIST } from '../../modules/pfr/agat/spfilterfeaturefromkilllist/main' +include { GFFCOMPARE as VALIDATE_PURGING_BY_AGAT } from '../../modules/nf-core/gffcompare/main' +include { AGAT_SPMERGEANNOTATIONS as MERGE_BRAKER_LIFTOFF } from '../../modules/pfr/agat/spmergeannotations/main' + +workflow PURGE_BREAKER_MODELS { + take: + braker_gff3 // [ meta, gff3 ] + braker_hints // [ meta, gff ] + liftoff_gff3 // [ meta, gff3 ] + tsebra_config // val(tsebra_config) + + main: + ch_versions = Channel.empty() + + // MODULE: AGAT_CONVERTSPGFF2GTF + AGAT_CONVERTSPGFF2GTF ( braker_gff3 ) + + ch_braker_gtf = AGAT_CONVERTSPGFF2GTF.out.output_gtf + ch_versions = ch_versions.mix(AGAT_CONVERTSPGFF2GTF.out.versions.first()) + + // COLLECTFILE: Prepare for TSEBRA + ch_tsebra_input_gtf = ch_braker_gtf + | map { meta, gtf -> + + def lines = gtf.readLines() + .collect { line -> + if ( line.startsWith('#') ) { return line } + + def cols = line.split('\t') + def feat = cols[2] + + if ( ! ( feat in [ 'gene', 'transcript', 'mRNA' ] ) ) { return line } + + def atts = cols[8] + def matches = atts =~ /ID ([^;]*)/ + def id = matches[0][1] + + def feat_format = ( feat == 'mRNA' ) ? 'transcript' : feat + + return ( cols[0..1] + [ feat_format ] + cols[3..7] + [ id ] ).join('\t') + }.join('\n') + + [ "${meta.id}.clean.gtf" ] + [ lines ] + } + | collectFile(newLine: true) + | map { file -> + [ [ id: file.baseName.replace(".clean", "") ], file ] + } + + // MODULE: TSEBRA + ch_tsebra_inputs = ch_tsebra_input_gtf + | join(braker_hints) + | combine(Channel.fromPath(tsebra_config)) + TSEBRA( + ch_tsebra_inputs.map { meta, gtf, gff, cfg -> [ meta, [ gtf ] ] }, + ch_tsebra_inputs.map { meta, gtf, gff, cfg -> [ gff ] }, + [], + ch_tsebra_inputs.map { meta, gtf, gff, cfg -> cfg } + ) + + ch_tsebra_gtf = TSEBRA.out.tsebra_gtf + ch_versions = ch_versions.mix(TSEBRA.out.versions.first()) + + // COLLECTFILE: Format TSEBRA output + ch_tsebra_formatted_gtf = ch_tsebra_gtf + | map { meta, gtf -> + + def lines = gtf.readLines() + .collect { line -> + if ( line.startsWith('#') ) { return line } + + def cols = line.split('\t') + def atts_r = '' + // Remove attributes and use AGAT_CONVERTSPGXF2GXF + // to create attributes based on sequential layout + + return ( cols[0..7] + [ atts_r ] ).join('\t') + }.join('\n') + + [ "${meta.id}.gtf" ] + [ lines ] + } + | collectFile(newLine: true) + | map { file -> + [ [ id: file.baseName ], file ] + } + + // MODULE: AGAT_CONVERTSPGXF2GXF + AGAT_CONVERTSPGXF2GXF ( ch_tsebra_formatted_gtf ) + + ch_tsebra_formatted_gff = AGAT_CONVERTSPGXF2GXF.out.output_gff + ch_versions = ch_versions.mix(AGAT_CONVERTSPGXF2GXF.out.versions.first()) + + // COLLECTFILE: Format AGAT_CONVERTSPGXF2GXF output + ch_tsebra_gff = ch_tsebra_formatted_gff + | map { meta, gtf -> + + def lines = gtf.readLines() + .collect { line -> + if ( line.startsWith('#') ) { return line } + + def cols = line.split('\t') + def atts_r = cols[8].replaceAll('-', '').replaceAll('agat', '') + + return ( cols[0..7] + [ atts_r ] ).join('\t') + }.join('\n') + + [ "${meta.id}.gff3" ] + [ lines ] + } + | collectFile(newLine: true) + | map { file -> + [ [ id: file.baseName ], file ] + } + + // MODULE: GFFCOMPARE as COMPARE_BRAKER_TO_LIFTOFF + ch_comparison_inputs = ch_tsebra_gff + | join(liftoff_gff3) + + + COMPARE_BRAKER_TO_LIFTOFF ( + ch_comparison_inputs.map { meta, braker, liftoff -> [ meta, braker ] }, + [ [], [], [] ], + ch_comparison_inputs.map { meta, braker, liftoff -> [ meta, liftoff ] }, + ) + + ch_tracking = COMPARE_BRAKER_TO_LIFTOFF.out.tracking + ch_versions = ch_versions.mix(COMPARE_BRAKER_TO_LIFTOFF.out.versions.first()) + + // COLLECTFILE: Transcript level kill list + ch_kill_list = ch_tracking + | map { meta, tracking -> + + def kept_lines = tracking.readLines() + .findAll { line -> + def cols = line.split('\t') + + ( cols[3] != 'u' ) && ( cols[3] != 'p' ) + } + + def tx_kill_list = kept_lines + .collect { line -> + def cols = line.split('\t') + + def matched = cols[4] =~ /q1:([^\|]+)\|([^\|]+)/ + + matched[0][2].trim() + }.join('\n') + + [ "${meta.id}.kill.list.txt" ] + tx_kill_list + } + | collectFile(newLine: true) + | map { file -> + [ [ id: file.baseName.replace('.kill.list', '') ], file ] + } + + // MODULE: AGAT_SPFILTERFEATUREFROMKILLLIST + ch_agat_kill_inputs = ch_tsebra_gff + | join(ch_kill_list) + + + AGAT_SPFILTERFEATUREFROMKILLLIST( + ch_agat_kill_inputs.map { meta, gff, kill -> [ meta, gff ] }, + ch_agat_kill_inputs.map { meta, gff, kill -> kill }, + [] // default config + ) + + ch_braker_purged_gff = AGAT_SPFILTERFEATUREFROMKILLLIST.out.gff + ch_versions = ch_versions.mix(AGAT_SPFILTERFEATUREFROMKILLLIST.out.versions.first()) + + // Handle case where liftoff is not present + ch_all_braker_gff = ch_tsebra_gff + | join(ch_braker_purged_gff, remainder:true) + | map { meta, tsebra_gff, purged_gff -> + if ( purged_gff ) { return [ meta, purged_gff ] } + if ( tsebra_gff ) { return [ meta, tsebra_gff ] } + } + + emit: + braker_purged_gff = ch_all_braker_gff // [ meta, gff3 ] + versions = ch_versions // [ versions.yml ] +} diff --git a/workflows/pangene.nf b/workflows/pangene.nf index 65d52d8..3d5e50d 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -6,7 +6,7 @@ include { ALIGN_RNASEQ } from '../subworkflows/local/al include { PREPARE_EXT_PROTS } from '../subworkflows/local/prepare_ext_prots' include { FASTA_BRAKER3 } from '../subworkflows/local/fasta_braker3' include { FASTA_LIFTOFF } from '../subworkflows/local/fasta_liftoff' -include { MERGE_ANNOTATIONS } from '../subworkflows/local/merge_annotations' +include { PURGE_BREAKER_MODELS } from '../subworkflows/local/purge_breaker_models' include { GFF_EGGNOGMAPPER } from '../subworkflows/local/gff_eggnogmapper' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions' @@ -124,8 +124,12 @@ workflow PANGENE { gff: [ [ id: idFromFileName( fastaFile.baseName ) ], file(gff, checkIfExists:true) ] } - ch_liftoff_fasta = ch_liftoff_mm.fasta - ch_liftoff_gff = ch_liftoff_mm.gff + ch_liftoff_fasta = ch_liftoff_mm.fasta + ch_liftoff_gff = ch_liftoff_mm.gff + + val_tsebra_config = params.braker_allow_isoforms + ? "${projectDir}/assets/tsebra-default.cfg" + : "${projectDir}/assets/tsebra-1form.cfg" // SUBWORKFLOW: PREPARE_ASSEMBLY PREPARE_ASSEMBLY( @@ -198,21 +202,25 @@ workflow PANGENE { ch_liftoff_gff3 = FASTA_LIFTOFF.out.gff3 ch_versions = ch_versions.mix(FASTA_LIFTOFF.out.versions) - // SUBWORKFLOW: MERGE_ANNOTATIONS - MERGE_ANNOTATIONS ( ch_braker_gff3, ch_liftoff_gff3 ) + // SUBWORKFLOW: PURGE_BREAKER_MODELS + PURGE_BREAKER_MODELS( + ch_braker_gff3, + ch_braker_hints, + ch_liftoff_gff3, + val_tsebra_config + ) - ch_merged_gff = MERGE_ANNOTATIONS.out.merged_gff - ch_braker_purged = MERGE_ANNOTATIONS.out.braker_purged - ch_versions = ch_versions.mix(MERGE_ANNOTATIONS.out.versions) + ch_braker_purged_gff = PURGE_BREAKER_MODELS.out.braker_purged_gff | view + ch_versions = ch_versions.mix(PURGE_BREAKER_MODELS.out.versions) - // SUBWORKFLOW: GFF_EGGNOGMAPPER - GFF_EGGNOGMAPPER( - ch_braker_purged, - ch_valid_target_assembly, - params.eggnogmapper_db_dir, - ) + // // SUBWORKFLOW: GFF_EGGNOGMAPPER + // GFF_EGGNOGMAPPER( + // ch_braker_purged, + // ch_valid_target_assembly, + // params.eggnogmapper_db_dir, + // ) - ch_versions = ch_versions.mix(GFF_EGGNOGMAPPER.out.versions) + // ch_versions = ch_versions.mix(GFF_EGGNOGMAPPER.out.versions) // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( From 22e609ccacc019507d56e28f78d492d2c2199b72 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 12 Apr 2024 13:12:29 +1200 Subject: [PATCH 20/40] Updated modules and subworkflows --- .editorconfig | 17 ++++++++++++++++ modules.json | 20 +++++++++---------- modules/nf-core/fastqc/main.nf | 6 ++++++ .../tests/main.nf.test | 2 +- .../spmergeannotations/tests/main.nf.test | 2 +- .../custom/restoregffids/tests/main.nf.test | 2 +- .../custom/shortenfastaids/tests/main.nf.test | 2 +- modules/pfr/edta/edta/tests/main.nf.test | 2 +- .../pfr/ltrretriever/lai/tests/main.nf.test | 2 +- .../pfr/repeatmodeler/builddatabase/main.nf | 4 ++-- .../builddatabase/tests/main.nf.test | 2 +- .../pfr/repeatmodeler/repeatmodeler/main.nf | 4 ++-- .../repeatmodeler/tests/main.nf.test | 2 +- .../pfr/fasta_edta_lai/tests/main.nf.test | 2 +- 14 files changed, 46 insertions(+), 23 deletions(-) diff --git a/.editorconfig b/.editorconfig index 24ab7a6..82dc73a 100644 --- a/.editorconfig +++ b/.editorconfig @@ -28,6 +28,23 @@ trim_trailing_whitespace = unset indent_style = unset indent_size = unset +# These files are edited and tested upstream in pfr/modules +[/modules/pfr/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset +indent_size = unset + +[/subworkflows/pfr/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset +indent_size = unset + [/assets/email*] indent_size = unset diff --git a/modules.json b/modules.json index da86263..a4300b1 100644 --- a/modules.json +++ b/modules.json @@ -7,42 +7,42 @@ "pfr": { "agat/spfilterfeaturefromkilllist": { "branch": "main", - "git_sha": "7a9a63b7727b0d3b3943f217809059e8116cee2b", + "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e", "installed_by": ["modules"] }, "agat/spmergeannotations": { "branch": "main", - "git_sha": "e2ee3129519098451ef60f8a8ca3b9efcecf2d69", + "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e", "installed_by": ["modules"] }, "custom/restoregffids": { "branch": "main", - "git_sha": "e9f6bdd634bdbcd52c5568ba82f16176ec06631f", + "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e", "installed_by": ["fasta_edta_lai"] }, "custom/shortenfastaids": { "branch": "main", - "git_sha": "5e0e41b51d7fc7f68ae43692b6fe19b95d7f3a8c", + "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e", "installed_by": ["fasta_edta_lai"] }, "edta/edta": { "branch": "main", - "git_sha": "35468dbb1f35eb17a43d7e05544601c7c3f8cd90", + "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e", "installed_by": ["fasta_edta_lai"] }, "ltrretriever/lai": { "branch": "main", - "git_sha": "154661d7c1769532ff7b5f11259644ec200dd47d", + "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e", "installed_by": ["fasta_edta_lai"] }, "repeatmodeler/builddatabase": { "branch": "main", - "git_sha": "9da0567f685b2772f65290f2bd6d6347671c8310", + "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e", "installed_by": ["modules"] }, "repeatmodeler/repeatmodeler": { "branch": "main", - "git_sha": "9da0567f685b2772f65290f2bd6d6347671c8310", + "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e", "installed_by": ["modules"] }, "tsebra": { @@ -56,7 +56,7 @@ "pfr": { "fasta_edta_lai": { "branch": "main", - "git_sha": "ba9c14555adf50c193f326dc91f48b770fe9b1ae", + "git_sha": "10b046eaac396f279c08e7e2bb067482ccd9c74e", "installed_by": ["subworkflows"] } } @@ -123,7 +123,7 @@ }, "fastqc": { "branch": "master", - "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", + "git_sha": "285a50500f9e02578d90b3ce6382ea3c30216acd", "installed_by": ["fastq_fastqc_umitools_fastp", "modules"] }, "gffcompare": { diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index 9e19a74..d79f1c8 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -25,6 +25,11 @@ process FASTQC { def old_new_pairs = reads instanceof Path || reads.size() == 1 ? [[ reads, "${prefix}.${reads.extension}" ]] : reads.withIndex().collect { entry, index -> [ entry, "${prefix}_${index + 1}.${entry.extension}" ] } def rename_to = old_new_pairs*.join(' ').join(' ') def renamed_files = old_new_pairs.collect{ old_name, new_name -> new_name }.join(' ') + + def memory_in_mb = MemoryUnit.of("${task.memory}").toUnit('MB') + // FastQC memory value allowed range (100 - 10000) + def fastqc_memory = memory_in_mb > 10000 ? 10000 : (memory_in_mb < 100 ? 100 : memory_in_mb) + """ printf "%s %s\\n" $rename_to | while read old_name new_name; do [ -f "\${new_name}" ] || ln -s \$old_name \$new_name @@ -33,6 +38,7 @@ process FASTQC { fastqc \\ $args \\ --threads $task.cpus \\ + --memory $fastqc_memory \\ $renamed_files cat <<-END_VERSIONS > versions.yml diff --git a/modules/pfr/agat/spfilterfeaturefromkilllist/tests/main.nf.test b/modules/pfr/agat/spfilterfeaturefromkilllist/tests/main.nf.test index 8f04e58..891e0a7 100644 --- a/modules/pfr/agat/spfilterfeaturefromkilllist/tests/main.nf.test +++ b/modules/pfr/agat/spfilterfeaturefromkilllist/tests/main.nf.test @@ -71,4 +71,4 @@ nextflow_process { } -} +} \ No newline at end of file diff --git a/modules/pfr/agat/spmergeannotations/tests/main.nf.test b/modules/pfr/agat/spmergeannotations/tests/main.nf.test index 78f2eba..5e25599 100644 --- a/modules/pfr/agat/spmergeannotations/tests/main.nf.test +++ b/modules/pfr/agat/spmergeannotations/tests/main.nf.test @@ -68,4 +68,4 @@ nextflow_process { } -} +} \ No newline at end of file diff --git a/modules/pfr/custom/restoregffids/tests/main.nf.test b/modules/pfr/custom/restoregffids/tests/main.nf.test index 521b924..cc374b7 100644 --- a/modules/pfr/custom/restoregffids/tests/main.nf.test +++ b/modules/pfr/custom/restoregffids/tests/main.nf.test @@ -60,4 +60,4 @@ nextflow_process { } -} +} \ No newline at end of file diff --git a/modules/pfr/custom/shortenfastaids/tests/main.nf.test b/modules/pfr/custom/shortenfastaids/tests/main.nf.test index dc46bae..efff639 100644 --- a/modules/pfr/custom/shortenfastaids/tests/main.nf.test +++ b/modules/pfr/custom/shortenfastaids/tests/main.nf.test @@ -128,4 +128,4 @@ nextflow_process { } -} +} \ No newline at end of file diff --git a/modules/pfr/edta/edta/tests/main.nf.test b/modules/pfr/edta/edta/tests/main.nf.test index 3aed0a2..7601876 100644 --- a/modules/pfr/edta/edta/tests/main.nf.test +++ b/modules/pfr/edta/edta/tests/main.nf.test @@ -74,4 +74,4 @@ nextflow_process { } -} +} \ No newline at end of file diff --git a/modules/pfr/ltrretriever/lai/tests/main.nf.test b/modules/pfr/ltrretriever/lai/tests/main.nf.test index df7db2c..a617811 100644 --- a/modules/pfr/ltrretriever/lai/tests/main.nf.test +++ b/modules/pfr/ltrretriever/lai/tests/main.nf.test @@ -163,4 +163,4 @@ nextflow_process { } -} +} \ No newline at end of file diff --git a/modules/pfr/repeatmodeler/builddatabase/main.nf b/modules/pfr/repeatmodeler/builddatabase/main.nf index 486e25d..6fe244b 100644 --- a/modules/pfr/repeatmodeler/builddatabase/main.nf +++ b/modules/pfr/repeatmodeler/builddatabase/main.nf @@ -26,7 +26,7 @@ process REPEATMODELER_BUILDDATABASE { cat <<-END_VERSIONS > versions.yml "${task.process}": - repeatmodeler: \$(RepeatModeler | grep '/usr/local/bin/RepeatModeler - ' | sed 's|/usr/local/bin/RepeatModeler - ||') + repeatmodeler: \$(RepeatModeler --version | sed 's/RepeatModeler version //') END_VERSIONS """ @@ -44,7 +44,7 @@ process REPEATMODELER_BUILDDATABASE { cat <<-END_VERSIONS > versions.yml "${task.process}": - repeatmodeler: \$(RepeatModeler | grep '/usr/local/bin/RepeatModeler - ' | sed 's|/usr/local/bin/RepeatModeler - ||') + repeatmodeler: \$(RepeatModeler --version | sed 's/RepeatModeler version //') END_VERSIONS """ } diff --git a/modules/pfr/repeatmodeler/builddatabase/tests/main.nf.test b/modules/pfr/repeatmodeler/builddatabase/tests/main.nf.test index 616f88c..fdeda4a 100644 --- a/modules/pfr/repeatmodeler/builddatabase/tests/main.nf.test +++ b/modules/pfr/repeatmodeler/builddatabase/tests/main.nf.test @@ -57,4 +57,4 @@ nextflow_process { } -} +} \ No newline at end of file diff --git a/modules/pfr/repeatmodeler/repeatmodeler/main.nf b/modules/pfr/repeatmodeler/repeatmodeler/main.nf index 34df322..c7df9ca 100644 --- a/modules/pfr/repeatmodeler/repeatmodeler/main.nf +++ b/modules/pfr/repeatmodeler/repeatmodeler/main.nf @@ -35,7 +35,7 @@ process REPEATMODELER_REPEATMODELER { cat <<-END_VERSIONS > versions.yml "${task.process}": - repeatmodeler: \$(RepeatModeler | grep '/usr/local/bin/RepeatModeler - ' | sed 's|/usr/local/bin/RepeatModeler - ||') + repeatmodeler: \$(RepeatModeler --version | sed 's/RepeatModeler version //') END_VERSIONS """ @@ -48,7 +48,7 @@ process REPEATMODELER_REPEATMODELER { cat <<-END_VERSIONS > versions.yml "${task.process}": - repeatmodeler: \$(RepeatModeler | grep '/usr/local/bin/RepeatModeler - ' | sed 's|/usr/local/bin/RepeatModeler - ||') + repeatmodeler: \$(RepeatModeler --version | sed 's/RepeatModeler version //') END_VERSIONS """ } diff --git a/modules/pfr/repeatmodeler/repeatmodeler/tests/main.nf.test b/modules/pfr/repeatmodeler/repeatmodeler/tests/main.nf.test index 78b7957..dd7185f 100644 --- a/modules/pfr/repeatmodeler/repeatmodeler/tests/main.nf.test +++ b/modules/pfr/repeatmodeler/repeatmodeler/tests/main.nf.test @@ -89,4 +89,4 @@ nextflow_process { } -} +} \ No newline at end of file diff --git a/subworkflows/pfr/fasta_edta_lai/tests/main.nf.test b/subworkflows/pfr/fasta_edta_lai/tests/main.nf.test index d968790..2c6850d 100644 --- a/subworkflows/pfr/fasta_edta_lai/tests/main.nf.test +++ b/subworkflows/pfr/fasta_edta_lai/tests/main.nf.test @@ -90,4 +90,4 @@ nextflow_workflow { ) } } -} +} \ No newline at end of file From f2d333c8e38115809281ce8582b29fd0ccd8b4a1 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 12 Apr 2024 13:30:32 +1200 Subject: [PATCH 21/40] Updated eggnogmapper --- conf/modules.config | 3 +-- modules/nf-core/eggnogmapper/meta.yml | 3 +++ workflows/pangene.nf | 16 ++++++++-------- 3 files changed, 12 insertions(+), 10 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index b00c35a..bb303ba 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -199,8 +199,7 @@ process { '--mp_start_method fork', "--itype proteins", params.eggnogmapper_tax_scope ? "--tax_scope ${params.eggnogmapper_tax_scope}" : '', - '--go_evidence all', - '--report_orthologs' + '--go_evidence all' ].join(' ').trim() } diff --git a/modules/nf-core/eggnogmapper/meta.yml b/modules/nf-core/eggnogmapper/meta.yml index 76d8aa2..b07c27e 100644 --- a/modules/nf-core/eggnogmapper/meta.yml +++ b/modules/nf-core/eggnogmapper/meta.yml @@ -60,3 +60,6 @@ output: pattern: "versions.yml" authors: - "@vagkaratzas" +maintainers: + - "@vagkaratzas" + - "@gallvp" diff --git a/workflows/pangene.nf b/workflows/pangene.nf index 3d5e50d..d5bce92 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -213,14 +213,14 @@ workflow PANGENE { ch_braker_purged_gff = PURGE_BREAKER_MODELS.out.braker_purged_gff | view ch_versions = ch_versions.mix(PURGE_BREAKER_MODELS.out.versions) - // // SUBWORKFLOW: GFF_EGGNOGMAPPER - // GFF_EGGNOGMAPPER( - // ch_braker_purged, - // ch_valid_target_assembly, - // params.eggnogmapper_db_dir, - // ) - - // ch_versions = ch_versions.mix(GFF_EGGNOGMAPPER.out.versions) + // SUBWORKFLOW: GFF_EGGNOGMAPPER + GFF_EGGNOGMAPPER( + ch_braker_purged, + ch_valid_target_assembly, + params.eggnogmapper_db_dir, + ) + + ch_versions = ch_versions.mix(GFF_EGGNOGMAPPER.out.versions) // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( From 3acaaac3c271dd9beb0f663819b270aaa4229d42 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 12 Apr 2024 13:32:44 +1200 Subject: [PATCH 22/40] Patched eggnogmapper and gffread --- modules.json | 6 +- .../nf-core/eggnogmapper/eggnogmapper.diff | 53 ++ modules/nf-core/gffread/gffread.diff | 675 ++++++++++++++++++ 3 files changed, 732 insertions(+), 2 deletions(-) create mode 100644 modules/nf-core/eggnogmapper/eggnogmapper.diff create mode 100644 modules/nf-core/gffread/gffread.diff diff --git a/modules.json b/modules.json index a4300b1..27dc611 100644 --- a/modules.json +++ b/modules.json @@ -109,7 +109,8 @@ "eggnogmapper": { "branch": "master", "git_sha": "9be0d5f9aeb31bded6780b5b589de7158ccf2c7b", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/eggnogmapper/eggnogmapper.diff" }, "fastavalidator": { "branch": "master", @@ -134,7 +135,8 @@ "gffread": { "branch": "master", "git_sha": "b1b959609bda44341120aed1766329909f54b8d0", - "installed_by": ["modules"] + "installed_by": ["modules"], + "patch": "modules/nf-core/gffread/gffread.diff" }, "gt/gff3": { "branch": "master", diff --git a/modules/nf-core/eggnogmapper/eggnogmapper.diff b/modules/nf-core/eggnogmapper/eggnogmapper.diff new file mode 100644 index 0000000..b38223d --- /dev/null +++ b/modules/nf-core/eggnogmapper/eggnogmapper.diff @@ -0,0 +1,53 @@ +Changes in module 'nf-core/eggnogmapper' +--- modules/nf-core/eggnogmapper/meta.yml ++++ modules/nf-core/eggnogmapper/meta.yml +@@ -60,3 +60,6 @@ + pattern: "versions.yml" + authors: + - "@vagkaratzas" ++maintainers: ++ - "@vagkaratzas" ++ - "@gallvp" + +--- modules/nf-core/eggnogmapper/main.nf ++++ modules/nf-core/eggnogmapper/main.nf +@@ -1,6 +1,6 @@ + process EGGNOGMAPPER { + tag "$meta.id" +- label 'process_long' ++ label 'process_high' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? +@@ -23,11 +23,13 @@ + task.ext.when == null || task.ext.when + + script: +- def args = task.ext.args ?: '' +- def prefix = task.ext.prefix ?: "${meta.id}" +- def is_compressed = fasta.name.endsWith(".gz") +- def fasta_name = fasta.name.replace(".gz", "") +- def dbmem = task.memory.toMega() > 40000 ? '--dbmem' : '' ++ def args = task.ext.args ?: '' ++ def prefix = task.ext.prefix ?: "${meta.id}" ++ def is_compressed = fasta.name.endsWith(".gz") ++ def fasta_name = fasta.name.replace(".gz", "") ++ def dbmem = task.memory.toMega() > 40000 ? '--dbmem' : '' ++ def database_arg = eggnog_db ? "--database $eggnog_db" : '' ++ def dmnd_db_arg = eggnog_diamond_db ? "--dmnd_db $eggnog_diamond_db" : '' + """ + if [ "$is_compressed" == "true" ]; then + gzip -c -d $fasta > $fasta_name +@@ -38,8 +40,8 @@ + -i ${fasta_name} \\ + --data_dir ${eggnog_data_dir} \\ + -m diamond \\ +- --dmnd_db ${eggnog_diamond_db} \\ +- --database ${eggnog_db} \\ ++ $dmnd_db_arg \\ ++ $database_arg \\ + --output ${prefix} \\ + ${dbmem} \\ + $args + +************************************************************ diff --git a/modules/nf-core/gffread/gffread.diff b/modules/nf-core/gffread/gffread.diff new file mode 100644 index 0000000..fa3668c --- /dev/null +++ b/modules/nf-core/gffread/gffread.diff @@ -0,0 +1,675 @@ +Changes in module 'nf-core/gffread' +--- modules/nf-core/gffread/environment.yml ++++ modules/nf-core/gffread/environment.yml +@@ -4,4 +4,4 @@ + - bioconda + - defaults + dependencies: +- - bioconda::gffread=0.12.1 ++ - bioconda::gffread=0.12.7 + +--- modules/nf-core/gffread/meta.yml ++++ modules/nf-core/gffread/meta.yml +@@ -13,11 +13,25 @@ + doi: 10.12688/f1000research.23297.1 + licence: ["MIT"] + input: ++ - meta: ++ type: map ++ description: | ++ Groovy Map containing meta data ++ e.g. [ id:'test' ] + - gff: + type: file + description: A reference file in either the GFF3, GFF2 or GTF format. + pattern: "*.{gff, gtf}" ++ - fasta: ++ type: file ++ description: A multi-fasta file with the genomic sequences ++ pattern: "*.{fasta,fa,faa,fas,fsa}" + output: ++ - meta: ++ type: map ++ description: | ++ Groovy Map containing meta data ++ e.g. [ id:'test' ] + - gtf: + type: file + description: GTF file resulting from the conversion of the GFF input file if '-T' argument is present +@@ -25,7 +39,11 @@ + - gffread_gff: + type: file + description: GFF3 file resulting from the conversion of the GFF input file if '-T' argument is absent +- pattern: "*.{gff3}" ++ pattern: "*.gff3" ++ - gffread_fasta: ++ type: file ++ description: Fasta file produced when either of '-w', '-x', '-y' parameters is present ++ pattern: "*.fasta" + - versions: + type: file + description: File containing software versions +@@ -34,3 +52,4 @@ + - "@edmundmiller" + maintainers: + - "@edmundmiller" ++ - "@gallvp" + +--- modules/nf-core/gffread/main.nf ++++ modules/nf-core/gffread/main.nf +@@ -1,32 +1,59 @@ + process GFFREAD { +- tag "$gff" ++ tag "$meta.id" + label 'process_low' + + conda "${moduleDir}/environment.yml" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? +- 'https://depot.galaxyproject.org/singularity/gffread:0.12.1--h8b12597_0' : +- 'biocontainers/gffread:0.12.1--h8b12597_0' }" ++ 'https://depot.galaxyproject.org/singularity/gffread:0.12.7--hdcf5f25_4' : ++ 'biocontainers/gffread:0.12.7--hdcf5f25_4' }" + + input: +- path gff ++ tuple val(meta), path(gff) ++ path fasta + + output: +- path "*.gtf" , emit: gtf , optional: true +- path "*.gff3" , emit: gffread_gff , optional: true +- path "versions.yml" , emit: versions ++ tuple val(meta), path("*.gtf") , emit: gtf , optional: true ++ tuple val(meta), path("*.gff3") , emit: gffread_gff , optional: true ++ tuple val(meta), path("*.fasta"), emit: gffread_fasta , optional: true ++ path "versions.yml" , emit: versions + + when: + task.ext.when == null || task.ext.when + + script: +- def args = task.ext.args ?: '' +- def prefix = task.ext.prefix ?: "${gff.baseName}" +- def extension = args.contains("-T") ? 'gtf' : 'gffread.gff3' ++ def args = task.ext.args ?: '' ++ def prefix = task.ext.prefix ?: "${meta.id}" ++ def extension = args.contains("-T") ? 'gtf' : ( ( ['-w', '-x', '-y' ].any { args.contains(it) } ) ? 'fasta' : 'gff3' ) ++ def fasta_arg = fasta ? "-g $fasta" : '' ++ def output_name = "${prefix}.${extension}" ++ def output = extension == "fasta" ? "$output_name" : "-o $output_name" ++ def args_sorted = args.replaceAll(/(.*)(-[wxy])(.*)/) { all, pre, param, post -> "$pre $post $param" }.trim() ++ if ( "$output_name" in [ "$gff", "$fasta" ] ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" + """ + gffread \\ + $gff \\ +- $args \\ +- -o ${prefix}.${extension} ++ $fasta_arg \\ ++ $args_sorted \\ ++ $output ++ ++ cat <<-END_VERSIONS > versions.yml ++ "${task.process}": ++ gffread: \$(gffread --version 2>&1) ++ END_VERSIONS ++ """ ++ ++ stub: ++ def args = task.ext.args ?: '' ++ def prefix = task.ext.prefix ?: "${meta.id}" ++ def extension = args.contains("-T") ? 'gtf' : ( ( ['-w', '-x', '-y' ].any { args.contains(it) } ) ? 'fasta' : 'gff3' ) ++ def fasta_arg = fasta ? "-g $fasta" : '' ++ def output_name = "${prefix}.${extension}" ++ def output = extension == "fasta" ? "$output_name" : "-o $output_name" ++ def args_sorted = args.replaceAll(/(.*)(-[wxy])(.*)/) { all, pre, param, post -> "$pre $post $param" }.trim() ++ if ( "$output_name" in [ "$gff", "$fasta" ] ) error "Input and output names are the same, use \"task.ext.prefix\" to disambiguate!" ++ """ ++ touch $output_name ++ + cat <<-END_VERSIONS > versions.yml + "${task.process}": + gffread: \$(gffread --version 2>&1) + +--- modules/nf-core/gffread/tests/main.nf.test.snap ++++ modules/nf-core/gffread/tests/main.nf.test.snap +@@ -1,24 +1,272 @@ + { + "sarscov2-gff3-gtf": { + "content": [ +- [ +- "genome.gtf:md5,2394072d7d31530dfd590c4a117bf6e3" +- ], +- [ +- "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" +- ] +- ], +- "timestamp": "2024-01-23T20:00:32.688779117" ++ { ++ "0": [ ++ [ ++ { ++ "id": "test" ++ }, ++ "test.gtf:md5,1ea0ae98d3388e0576407dc4a24ef428" ++ ] ++ ], ++ "1": [ ++ ++ ], ++ "2": [ ++ ++ ], ++ "3": [ ++ "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" ++ ], ++ "gffread_fasta": [ ++ ++ ], ++ "gffread_gff": [ ++ ++ ], ++ "gtf": [ ++ [ ++ { ++ "id": "test" ++ }, ++ "test.gtf:md5,1ea0ae98d3388e0576407dc4a24ef428" ++ ] ++ ], ++ "versions": [ ++ "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" ++ ] ++ } ++ ], ++ "meta": { ++ "nf-test": "0.8.4", ++ "nextflow": "23.10.1" ++ }, ++ "timestamp": "2024-04-09T10:48:56.496187" + }, + "sarscov2-gff3-gff3": { + "content": [ +- [ +- "genome.gffread.gff3:md5,a7d40d99dcddac23ac673c473279ea2d" +- ], +- [ +- "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" +- ] +- ], +- "timestamp": "2024-01-23T20:07:11.457356625" ++ { ++ "0": [ ++ ++ ], ++ "1": [ ++ [ ++ { ++ "id": "test" ++ }, ++ "test.gff3:md5,c4e5da6267c6bee5899a2c204ae1ad91" ++ ] ++ ], ++ "2": [ ++ ++ ], ++ "3": [ ++ "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" ++ ], ++ "gffread_fasta": [ ++ ++ ], ++ "gffread_gff": [ ++ [ ++ { ++ "id": "test" ++ }, ++ "test.gff3:md5,c4e5da6267c6bee5899a2c204ae1ad91" ++ ] ++ ], ++ "gtf": [ ++ ++ ], ++ "versions": [ ++ "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" ++ ] ++ } ++ ], ++ "meta": { ++ "nf-test": "0.8.4", ++ "nextflow": "23.10.1" ++ }, ++ "timestamp": "2024-04-09T10:49:00.892782" ++ }, ++ "sarscov2-gff3-gtf-stub": { ++ "content": [ ++ { ++ "0": [ ++ [ ++ { ++ "id": "test" ++ }, ++ "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e" ++ ] ++ ], ++ "1": [ ++ ++ ], ++ "2": [ ++ ++ ], ++ "3": [ ++ "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" ++ ], ++ "gffread_fasta": [ ++ ++ ], ++ "gffread_gff": [ ++ ++ ], ++ "gtf": [ ++ [ ++ { ++ "id": "test" ++ }, ++ "test.gtf:md5,d41d8cd98f00b204e9800998ecf8427e" ++ ] ++ ], ++ "versions": [ ++ "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" ++ ] ++ } ++ ], ++ "meta": { ++ "nf-test": "0.8.4", ++ "nextflow": "23.10.1" ++ }, ++ "timestamp": "2024-04-09T11:11:26.975666" ++ }, ++ "sarscov2-gff3-fasta-stub": { ++ "content": [ ++ { ++ "0": [ ++ ++ ], ++ "1": [ ++ ++ ], ++ "2": [ ++ [ ++ { ++ "id": "test" ++ }, ++ "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" ++ ] ++ ], ++ "3": [ ++ "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" ++ ], ++ "gffread_fasta": [ ++ [ ++ { ++ "id": "test" ++ }, ++ "test.fasta:md5,d41d8cd98f00b204e9800998ecf8427e" ++ ] ++ ], ++ "gffread_gff": [ ++ ++ ], ++ "gtf": [ ++ ++ ], ++ "versions": [ ++ "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" ++ ] ++ } ++ ], ++ "meta": { ++ "nf-test": "0.8.4", ++ "nextflow": "23.10.1" ++ }, ++ "timestamp": "2024-04-09T11:11:44.34792" ++ }, ++ "sarscov2-gff3-gff3-stub": { ++ "content": [ ++ { ++ "0": [ ++ ++ ], ++ "1": [ ++ [ ++ { ++ "id": "test" ++ }, ++ "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" ++ ] ++ ], ++ "2": [ ++ ++ ], ++ "3": [ ++ "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" ++ ], ++ "gffread_fasta": [ ++ ++ ], ++ "gffread_gff": [ ++ [ ++ { ++ "id": "test" ++ }, ++ "test.gff3:md5,d41d8cd98f00b204e9800998ecf8427e" ++ ] ++ ], ++ "gtf": [ ++ ++ ], ++ "versions": [ ++ "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" ++ ] ++ } ++ ], ++ "meta": { ++ "nf-test": "0.8.4", ++ "nextflow": "23.10.1" ++ }, ++ "timestamp": "2024-04-09T11:11:35.221671" ++ }, ++ "sarscov2-gff3-fasta": { ++ "content": [ ++ { ++ "0": [ ++ ++ ], ++ "1": [ ++ ++ ], ++ "2": [ ++ [ ++ { ++ "id": "test" ++ }, ++ "test.fasta:md5,5f8108fb51739a0588ccf0a251de919a" ++ ] ++ ], ++ "3": [ ++ "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" ++ ], ++ "gffread_fasta": [ ++ [ ++ { ++ "id": "test" ++ }, ++ "test.fasta:md5,5f8108fb51739a0588ccf0a251de919a" ++ ] ++ ], ++ "gffread_gff": [ ++ ++ ], ++ "gtf": [ ++ ++ ], ++ "versions": [ ++ "versions.yml:md5,05f671c6c6e530acedad0af0a5948dbd" ++ ] ++ } ++ ], ++ "meta": { ++ "nf-test": "0.8.4", ++ "nextflow": "23.10.1" ++ }, ++ "timestamp": "2024-04-09T10:54:02.88143" + } + } +--- modules/nf-core/gffread/tests/main.nf.test ++++ modules/nf-core/gffread/tests/main.nf.test +@@ -18,47 +18,203 @@ + } + process { + """ +- input[0] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) +- """ +- } +- } +- +- then { +- assertAll ( +- { assert process.success }, +- { assert snapshot( +- process.out.gtf, +- process.out.versions +- ).match() }, ++ input[0] = [ ++ [id: 'test'], ++ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) ++ ] ++ input[1] = [] ++ """ ++ } ++ } ++ ++ then { ++ assertAll ( ++ { assert process.success }, ++ { assert snapshot(process.out).match() }, ++ { assert process.out.gffread_gff == [] }, ++ { assert process.out.gffread_fasta == [] } ++ ) ++ } ++ ++ } ++ ++ test("sarscov2-gff3-gtf-stub") { ++ ++ options '-stub' ++ config "./nextflow.config" ++ ++ when { ++ params { ++ outdir = "$outputDir" ++ } ++ process { ++ """ ++ input[0] = [ ++ [id: 'test'], ++ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) ++ ] ++ input[1] = [] ++ """ ++ } ++ } ++ ++ then { ++ assertAll ( ++ { assert process.success }, ++ { assert snapshot(process.out).match() }, ++ { assert process.out.gffread_gff == [] }, ++ { assert process.out.gffread_fasta == [] } ++ ) ++ } ++ ++ } ++ ++ test("sarscov2-gff3-gff3") { ++ ++ config "./nextflow-gff3.config" ++ ++ when { ++ params { ++ outdir = "$outputDir" ++ } ++ process { ++ """ ++ input[0] = [ ++ [id: 'test'], ++ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) ++ ] ++ input[1] = [] ++ """ ++ } ++ } ++ ++ then { ++ assertAll ( ++ { assert process.success }, ++ { assert snapshot(process.out).match() }, ++ { assert process.out.gtf == [] }, ++ { assert process.out.gffread_fasta == [] } ++ ) ++ } ++ ++ } ++ ++ test("sarscov2-gff3-gff3-stub") { ++ ++ options '-stub' ++ config "./nextflow-gff3.config" ++ ++ when { ++ params { ++ outdir = "$outputDir" ++ } ++ process { ++ """ ++ input[0] = [ ++ [id: 'test'], ++ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) ++ ] ++ input[1] = [] ++ """ ++ } ++ } ++ ++ then { ++ assertAll ( ++ { assert process.success }, ++ { assert snapshot(process.out).match() }, ++ { assert process.out.gtf == [] }, ++ { assert process.out.gffread_fasta == [] } ++ ) ++ } ++ ++ } ++ ++ test("sarscov2-gff3-fasta") { ++ ++ config "./nextflow-fasta.config" ++ ++ when { ++ params { ++ outdir = "$outputDir" ++ } ++ process { ++ """ ++ input[0] = [ ++ [id: 'test'], ++ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) ++ ] ++ input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ++ """ ++ } ++ } ++ ++ then { ++ assertAll ( ++ { assert process.success }, ++ { assert snapshot(process.out).match() }, ++ { assert process.out.gtf == [] }, + { assert process.out.gffread_gff == [] } + ) + } + + } + +- test("sarscov2-gff3-gff3") { +- +- config "./nextflow-gff3.config" +- +- when { +- params { +- outdir = "$outputDir" +- } +- process { +- """ +- input[0] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) +- """ +- } +- } +- +- then { +- assertAll ( +- { assert process.success }, +- { assert snapshot( +- process.out.gffread_gff, +- process.out.versions +- ).match() }, +- { assert process.out.gtf == [] }, ++ test("sarscov2-gff3-fasta-stub") { ++ ++ options '-stub' ++ config "./nextflow-fasta.config" ++ ++ when { ++ params { ++ outdir = "$outputDir" ++ } ++ process { ++ """ ++ input[0] = [ ++ [id: 'test'], ++ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) ++ ] ++ input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ++ """ ++ } ++ } ++ ++ then { ++ assertAll ( ++ { assert process.success }, ++ { assert snapshot(process.out).match() }, ++ { assert process.out.gtf == [] }, ++ { assert process.out.gffread_gff == [] } ++ ) ++ } ++ ++ } ++ ++ test("sarscov2-gff3-fasta-fail-catch") { ++ ++ options '-stub' ++ config "./nextflow-fasta.config" ++ ++ when { ++ params { ++ outdir = "$outputDir" ++ } ++ process { ++ """ ++ input[0] = [ ++ [id: 'genome'], ++ file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.gff3", checkIfExists: true) ++ ] ++ input[1] = file(params.modules_testdata_base_path + "genomics/sarscov2/genome/genome.fasta", checkIfExists: true) ++ """ ++ } ++ } ++ ++ then { ++ assertAll ( ++ { assert ! process.success }, ++ { assert process.stdout.toString().contains("Input and output names are the same") } + ) + } + + +--- /dev/null ++++ modules/nf-core/gffread/tests/nextflow-fasta.config +@@ -0,0 +1,5 @@ ++process { ++ withName: GFFREAD { ++ ext.args = '-w -S' ++ } ++} + +************************************************************ From 9915ee81444638df2270a141013de560486cb443 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Fri, 12 Apr 2024 13:39:41 +1200 Subject: [PATCH 23/40] Added params eggnogmapper_evalue and eggnogmapper_pident --- CHANGELOG.md | 1 + conf/modules.config | 7 +++---- nextflow.config | 3 ++- nextflow_schema.json | 12 ++++++++++++ 4 files changed, 18 insertions(+), 5 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 24e46c4..5ec8324 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -33,6 +33,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 24. Added `GFF_EGGNOGMAPPER` sub-workflow 25. Now using a custom version of `GFFREAD` which supports `meta` and `fasta` 26. Now using TSEBRA to purge models which do not have full intron support from BRAKER hints +27. Added params `eggnogmapper_evalue` and `eggnogmapper_pident` ### `Fixed` diff --git a/conf/modules.config b/conf/modules.config index bb303ba..f1debae 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -194,13 +194,12 @@ process { withName: EGGNOGMAPPER { ext.args = [ - '--evalue 0.00001', - '--pident 35', + "--evalue $params.eggnogmapper_evalue", + "--pident $params.eggnogmapper_pident", + params.eggnogmapper_tax_scope ? "--tax_scope $params.eggnogmapper_tax_scope" : '', '--mp_start_method fork', "--itype proteins", - params.eggnogmapper_tax_scope ? "--tax_scope ${params.eggnogmapper_tax_scope}" : '', '--go_evidence all' - ].join(' ').trim() } } diff --git a/nextflow.config b/nextflow.config index c095d2d..7378ee5 100644 --- a/nextflow.config +++ b/nextflow.config @@ -37,7 +37,8 @@ params { liftoff_identity = 0.9 eggnogmapper_db_dir = null eggnogmapper_tax_scope = null - + eggnogmapper_evalue = 0.00001 + eggnogmapper_pident = 35 // Max job request options max_cpus = 12 diff --git a/nextflow_schema.json b/nextflow_schema.json index 2192cb1..0e0c559 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -193,6 +193,18 @@ "type": "integer", "description": "Eggnogmapper taxonomy scopre", "minimum": 0 + }, + "eggnogmapper_evalue": { + "type": "number", + "default": 1e-5, + "description": "Report only alignments below or equal the e-value threshold" + }, + "eggnogmapper_pident": { + "type": "integer", + "default": 35, + "description": "Report only alignments above or equal to the given percentage of identity (0-100)", + "minimum": 0, + "maximum": 100 } } }, From 0e5a43d06718cdcb393b5d10d23a73e6a8b46026 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 18 Apr 2024 09:30:10 +1200 Subject: [PATCH 24/40] Added PURGE_NOHIT_BRAKER_MODELS sub-workflow --- CHANGELOG.md | 3 +- TODO.md | 3 - conf/modules.config | 6 ++ nextflow.config | 1 + nextflow_schema.json | 9 +- subworkflows/local/gff_eggnogmapper.nf | 7 +- .../local/purge_nohit_braker_models.nf | 98 +++++++++++++++++++ workflows/pangene.nf | 16 ++- 8 files changed, 134 insertions(+), 9 deletions(-) create mode 100644 subworkflows/local/purge_nohit_braker_models.nf diff --git a/CHANGELOG.md b/CHANGELOG.md index 5ec8324..9589dc1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 0.2.0+dev - [12-April-2024] +## 0.2.0+dev - [18-April-2024] ### `Added` @@ -34,6 +34,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 25. Now using a custom version of `GFFREAD` which supports `meta` and `fasta` 26. Now using TSEBRA to purge models which do not have full intron support from BRAKER hints 27. Added params `eggnogmapper_evalue` and `eggnogmapper_pident` +28. Added `PURGE_NOHIT_BRAKER_MODELS` sub-workflow ### `Fixed` diff --git a/TODO.md b/TODO.md index 8e4fe71..e69de29 100644 --- a/TODO.md +++ b/TODO.md @@ -1,3 +0,0 @@ -- [ ] From Cecilia: - -> https://github.com/zhaotao1987/SynNet-Pipeline diff --git a/conf/modules.config b/conf/modules.config index f1debae..7ecd624 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -204,6 +204,12 @@ process { } } +process { + withName: '.*:PURGE_NOHIT_BRAKER_MODELS:AGAT_SPFILTERFEATUREFROMKILLLIST' { + ext.prefix = { "${meta.id}.nohits.purged" } + } +} + process { withName: 'CUSTOM_DUMPSOFTWAREVERSIONS' { publishDir = [ diff --git a/nextflow.config b/nextflow.config index 7378ee5..60b2dc2 100644 --- a/nextflow.config +++ b/nextflow.config @@ -39,6 +39,7 @@ params { eggnogmapper_tax_scope = null eggnogmapper_evalue = 0.00001 eggnogmapper_pident = 35 + eggnogmapper_purge_nohits = true // Max job request options max_cpus = 12 diff --git a/nextflow_schema.json b/nextflow_schema.json index 0e0c559..b1c5fd9 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -197,14 +197,19 @@ "eggnogmapper_evalue": { "type": "number", "default": 1e-5, - "description": "Report only alignments below or equal the e-value threshold" + "description": "Only report alignments below or equal the e-value threshold" }, "eggnogmapper_pident": { "type": "integer", "default": 35, - "description": "Report only alignments above or equal to the given percentage of identity (0-100)", + "description": "Only report alignments above or equal to the given percentage of identity (0-100)", "minimum": 0, "maximum": 100 + }, + "eggnogmapper_purge_nohits": { + "type": "boolean", + "default": true, + "description": "Purge predicted transcripts which do not have a hit against eggnog" } } }, diff --git a/subworkflows/local/gff_eggnogmapper.nf b/subworkflows/local/gff_eggnogmapper.nf index 59c5e3e..38d9673 100644 --- a/subworkflows/local/gff_eggnogmapper.nf +++ b/subworkflows/local/gff_eggnogmapper.nf @@ -8,7 +8,6 @@ workflow GFF_EGGNOGMAPPER { db_folder // val(db_folder) main: - // Versions ch_versions = Channel.empty() @@ -35,8 +34,14 @@ workflow GFF_EGGNOGMAPPER { [ [], [] ] ) + ch_eggnogmapper_annotations = EGGNOGMAPPER.out.annotations + ch_eggnogmapper_orthologs = EGGNOGMAPPER.out.orthologs + ch_eggnogmapper_hits = EGGNOGMAPPER.out.hits ch_versions = ch_versions.mix(EGGNOGMAPPER.out.versions.first()) emit: + eggnogmapper_annotations = ch_eggnogmapper_annotations + eggnogmapper_orthologs = ch_eggnogmapper_orthologs + eggnogmapper_hits = ch_eggnogmapper_hits versions = ch_versions } diff --git a/subworkflows/local/purge_nohit_braker_models.nf b/subworkflows/local/purge_nohit_braker_models.nf new file mode 100644 index 0000000..038380d --- /dev/null +++ b/subworkflows/local/purge_nohit_braker_models.nf @@ -0,0 +1,98 @@ +include { AGAT_SPFILTERFEATUREFROMKILLLIST } from '../../modules/pfr/agat/spfilterfeaturefromkilllist/main' + +workflow PURGE_NOHIT_BRAKER_MODELS { + take: + ch_braker_gff // [ meta, gff ] + ch_eggnogmapper_hits // [ meta, hits ] + val_purge_nohits // val(true|false) + + main: + ch_versions = Channel.empty() + + // COLLECTFILE: Transcript level kill list + ch_kill_list = ch_braker_gff + | join(ch_eggnogmapper_hits) + | map { meta, gff, hits -> + + def tx_with_hits = hits.readLines() + .collect { it.split('\t')[0] } + .sort(false) + .unique() + + def tx_in_gff = gff.readLines() + .findAll { it -> + if ( it.startsWith('#') ) { return false } + def feat = it.split('\t')[2] + ( feat == 'transcript' || feat == 'mRNA' ) + } + .collect { it -> + def attrs = it.split('\t')[8] + + ( attrs =~ /ID=([^;]*)/ )[0][1] + } + .sort(false) + .unique() + + def tx_without_hits = tx_in_gff - tx_with_hits + + [ "${meta.id}.kill.list.txt" ] + tx_without_hits.join('\n') + } + | collectFile(newLine: true) + | map { file -> + [ [ id: file.baseName.replace('.kill.list', '') ], file ] + } + + // MODULE: AGAT_SPFILTERFEATUREFROMKILLLIST + ch_agat_kill_inputs = ! val_purge_nohits + ? Channel.empty() + : ch_braker_gff + | join(ch_kill_list) + + + AGAT_SPFILTERFEATUREFROMKILLLIST( + ch_agat_kill_inputs.map { meta, gff, kill -> [ meta, gff ] }, + ch_agat_kill_inputs.map { meta, gff, kill -> kill }, + [] // default config + ) + + ch_braker_purged_gff = AGAT_SPFILTERFEATUREFROMKILLLIST.out.gff + ch_versions = ch_versions.mix(AGAT_SPFILTERFEATUREFROMKILLLIST.out.versions.first()) + + // COLLECTFILE: Mark transcripts with note=No%20eggnog%20hit + ch_marked_gff = val_purge_nohits + ? Channel.empty() + : ch_braker_gff + | join(ch_kill_list) + | map { meta, gff, lst -> + def tx_without_hits = lst.readLines().collect { "$it".trim() } + + def marked_gff_lines = gff.readLines() + .collect { line -> + + if ( line.startsWith('#') ) { return line } + + def cols = line.split('\t') + def feat = cols[2] + + if ( ! ( feat == 'transcript' || feat == 'mRNA' ) ) { return line } + + def atts = cols[8] + def matches = atts =~ /ID=([^;]*)/ + def tx_id = matches[0][1] + + if ( ! ( tx_id in tx_without_hits ) ) { return line } + + return ( line + ';note=No%20eggnog%20hit' ) + } + + [ "${meta.id}.marked.gff3" ] + marked_gff_lines.join('\n') + } + | collectFile(newLine: true) + | map { file -> + [ [ id: file.baseName.replace('.marked', '') ], file ] + } + + emit: + purged_or_marked_gff = ch_braker_purged_gff.mix(ch_marked_gff) + versions = ch_versions // [ versions.yml ] +} diff --git a/workflows/pangene.nf b/workflows/pangene.nf index d5bce92..6c0a747 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -8,6 +8,7 @@ include { FASTA_BRAKER3 } from '../subworkflows/local/fa include { FASTA_LIFTOFF } from '../subworkflows/local/fasta_liftoff' include { PURGE_BREAKER_MODELS } from '../subworkflows/local/purge_breaker_models' include { GFF_EGGNOGMAPPER } from '../subworkflows/local/gff_eggnogmapper' +include { PURGE_NOHIT_BRAKER_MODELS } from '../subworkflows/local/purge_nohit_braker_models' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions' log.info paramsSummaryLog(workflow) @@ -210,18 +211,29 @@ workflow PANGENE { val_tsebra_config ) - ch_braker_purged_gff = PURGE_BREAKER_MODELS.out.braker_purged_gff | view + ch_braker_purged_gff = PURGE_BREAKER_MODELS.out.braker_purged_gff ch_versions = ch_versions.mix(PURGE_BREAKER_MODELS.out.versions) // SUBWORKFLOW: GFF_EGGNOGMAPPER GFF_EGGNOGMAPPER( - ch_braker_purged, + ch_braker_purged_gff, ch_valid_target_assembly, params.eggnogmapper_db_dir, ) + ch_eggnogmapper_hits = GFF_EGGNOGMAPPER.out.eggnogmapper_hits ch_versions = ch_versions.mix(GFF_EGGNOGMAPPER.out.versions) + // SUBWORKFLOW: PURGE_NOHIT_BRAKER_MODELS + PURGE_NOHIT_BRAKER_MODELS( + ch_braker_purged_gff, + ch_eggnogmapper_hits, + params.eggnogmapper_purge_nohits + ) + + ch_braker_purged_marked_gff = PURGE_NOHIT_BRAKER_MODELS.out.purged_or_marked_gff + ch_versions = ch_versions.mix(PURGE_NOHIT_BRAKER_MODELS.out.versions) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From f1334538b8da2d389fdb9a3ab01b2ea7049799bc Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 18 Apr 2024 12:03:25 +1200 Subject: [PATCH 25/40] Removed liftoff models with valid_ORF=False --- CHANGELOG.md | 1 + conf/modules.config | 11 +++++- subworkflows/local/fasta_liftoff.nf | 57 ++++++++++++++++++++++++++++- workflows/pangene.nf | 38 +++++++++---------- 4 files changed, 85 insertions(+), 22 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 9589dc1..b2c020f 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -35,6 +35,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 26. Now using TSEBRA to purge models which do not have full intron support from BRAKER hints 27. Added params `eggnogmapper_evalue` and `eggnogmapper_pident` 28. Added `PURGE_NOHIT_BRAKER_MODELS` sub-workflow +29. Removed liftoff models with `valid_ORF=False` ### `Fixed` diff --git a/conf/modules.config b/conf/modules.config index 7ecd624..b9993a4 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -166,13 +166,22 @@ if(params.liftoff_annotations) { ].join(' ').trim() } - withName: GFFREAD_BEFORE_LIFTOFF { + withName: '.*:FASTA_LIFTOFF:GFFREAD_BEFORE_LIFTOFF' { ext.args = '--no-pseudo --keep-genes' } withName: MERGE_LIFTOFF_ANNOTATIONS { ext.prefix = { "${meta.id}.merged.liftoffs" } } + + withName: '.*:FASTA_LIFTOFF:AGAT_SPFILTERFEATUREFROMKILLLIST' { + ext.prefix = { "${meta.id}.invalid.orf.purged" } + } + + withName: '.*:FASTA_LIFTOFF:GFFREAD_AFTER_LIFTOFF' { + ext.prefix = { "${meta.id}.liftoff" } + ext.args = '--keep-genes' + } } } diff --git a/subworkflows/local/fasta_liftoff.nf b/subworkflows/local/fasta_liftoff.nf index e1933e8..01cd776 100644 --- a/subworkflows/local/fasta_liftoff.nf +++ b/subworkflows/local/fasta_liftoff.nf @@ -3,6 +3,8 @@ include { GUNZIP as GUNZIP_GFF } from '../../mo include { GFFREAD as GFFREAD_BEFORE_LIFTOFF } from '../../modules/nf-core/gffread/main' include { LIFTOFF } from '../../modules/nf-core/liftoff/main' include { AGAT_SPMERGEANNOTATIONS as MERGE_LIFTOFF_ANNOTATIONS } from '../../modules/pfr/agat/spmergeannotations/main' +include { AGAT_SPFILTERFEATUREFROMKILLLIST } from '../../modules/pfr/agat/spfilterfeaturefromkilllist/main' +include { GFFREAD as GFFREAD_AFTER_LIFTOFF } from '../../modules/nf-core/gffread/main' workflow FASTA_LIFTOFF { take: @@ -99,7 +101,58 @@ workflow FASTA_LIFTOFF { ch_merged_gff = MERGE_LIFTOFF_ANNOTATIONS.out.gff.mix(ch_merge_inputs.one) ch_versions = ch_versions.mix(MERGE_LIFTOFF_ANNOTATIONS.out.versions.first()) + // COLLECTFILE: Transcript level kill list + ch_kill_list = ch_merged_gff + | map { meta, gff -> + + def tx_from_gff = gff.readLines() + .findAll { it -> + if ( it.startsWith('#') ) { return false } + + def cols = it.split('\t') + def feat = cols[2] + if ( ! ( feat == 'transcript' || feat == 'mRNA' ) ) { return false } + + def attrs = cols[8] + attrs.contains('valid_ORF=False') + } + .collect { + def cols = it.split('\t') + def attrs = cols[8] + + def matches = attrs =~ /ID=([^;]*)/ + + return matches[0][1] + } + + [ "${meta.id}.kill.list.txt" ] + tx_from_gff.join('\n') + } + | collectFile(newLine: true) + | map { file -> + [ [ id: file.baseName.replace('.kill.list', '') ], file ] + } + + // MODULE: AGAT_SPFILTERFEATUREFROMKILLLIST + ch_agat_kill_inputs = ch_merged_gff + | join(ch_kill_list) + + + AGAT_SPFILTERFEATUREFROMKILLLIST( + ch_agat_kill_inputs.map { meta, gff, kill -> [ meta, gff ] }, + ch_agat_kill_inputs.map { meta, gff, kill -> kill }, + [] // default config + ) + + ch_liftoff_purged_gff = AGAT_SPFILTERFEATUREFROMKILLLIST.out.gff + ch_versions = ch_versions.mix(AGAT_SPFILTERFEATUREFROMKILLLIST.out.versions.first()) + + // MODULE: GFFREAD as GFFREAD_AFTER_LIFTOFF + GFFREAD_AFTER_LIFTOFF ( ch_liftoff_purged_gff, [] ) + + ch_attr_trimmed_gff = GFFREAD_AFTER_LIFTOFF.out.gffread_gff + ch_versions = ch_versions.mix(GFFREAD_AFTER_LIFTOFF.out.versions.first()) + emit: - gff3 = ch_merged_gff // [ meta, gff3 ] - versions = ch_versions // [ versions.yml ] + gff3 = ch_attr_trimmed_gff // [ meta, gff3 ] + versions = ch_versions // [ versions.yml ] } diff --git a/workflows/pangene.nf b/workflows/pangene.nf index 6c0a747..b2f8a7a 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -214,25 +214,25 @@ workflow PANGENE { ch_braker_purged_gff = PURGE_BREAKER_MODELS.out.braker_purged_gff ch_versions = ch_versions.mix(PURGE_BREAKER_MODELS.out.versions) - // SUBWORKFLOW: GFF_EGGNOGMAPPER - GFF_EGGNOGMAPPER( - ch_braker_purged_gff, - ch_valid_target_assembly, - params.eggnogmapper_db_dir, - ) - - ch_eggnogmapper_hits = GFF_EGGNOGMAPPER.out.eggnogmapper_hits - ch_versions = ch_versions.mix(GFF_EGGNOGMAPPER.out.versions) - - // SUBWORKFLOW: PURGE_NOHIT_BRAKER_MODELS - PURGE_NOHIT_BRAKER_MODELS( - ch_braker_purged_gff, - ch_eggnogmapper_hits, - params.eggnogmapper_purge_nohits - ) - - ch_braker_purged_marked_gff = PURGE_NOHIT_BRAKER_MODELS.out.purged_or_marked_gff - ch_versions = ch_versions.mix(PURGE_NOHIT_BRAKER_MODELS.out.versions) + // // SUBWORKFLOW: GFF_EGGNOGMAPPER + // GFF_EGGNOGMAPPER( + // ch_braker_purged_gff, + // ch_valid_target_assembly, + // params.eggnogmapper_db_dir, + // ) + + // ch_eggnogmapper_hits = GFF_EGGNOGMAPPER.out.eggnogmapper_hits + // ch_versions = ch_versions.mix(GFF_EGGNOGMAPPER.out.versions) + + // // SUBWORKFLOW: PURGE_NOHIT_BRAKER_MODELS + // PURGE_NOHIT_BRAKER_MODELS( + // ch_braker_purged_gff, + // ch_eggnogmapper_hits, + // params.eggnogmapper_purge_nohits + // ) + + // ch_braker_purged_marked_gff = PURGE_NOHIT_BRAKER_MODELS.out.purged_or_marked_gff + // ch_versions = ch_versions.mix(PURGE_NOHIT_BRAKER_MODELS.out.versions) // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( From 999236d391734c83737a377d311558c0089daf17 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 18 Apr 2024 12:35:09 +1200 Subject: [PATCH 26/40] Now merging BRAKER and liftoff models before running eggnogmapper --- CHANGELOG.md | 1 + conf/modules.config | 8 ++- nextflow.config | 2 +- nextflow_schema.json | 2 +- ...braker_models.nf => purge_nohit_models.nf} | 20 +++---- workflows/pangene.nf | 60 ++++++++++++------- 6 files changed, 60 insertions(+), 33 deletions(-) rename subworkflows/local/{purge_nohit_braker_models.nf => purge_nohit_models.nf} (88%) diff --git a/CHANGELOG.md b/CHANGELOG.md index b2c020f..90e24b2 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -36,6 +36,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 27. Added params `eggnogmapper_evalue` and `eggnogmapper_pident` 28. Added `PURGE_NOHIT_BRAKER_MODELS` sub-workflow 29. Removed liftoff models with `valid_ORF=False` +30. Now merging BRAKER and liftoff models before running eggnogmapper ### `Fixed` diff --git a/conf/modules.config b/conf/modules.config index b9993a4..9824dda 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -196,6 +196,12 @@ process { } } +process { + withName: '.*:PANGENE:AGAT_SPMERGEANNOTATIONS' { + ext.prefix = { "${meta.id}.liftoff.braker" } + } +} + process { withName: GFF2FASTA_FOR_EGGNOGMAPPER { ext.args = '-y' @@ -214,7 +220,7 @@ process { } process { - withName: '.*:PURGE_NOHIT_BRAKER_MODELS:AGAT_SPFILTERFEATUREFROMKILLLIST' { + withName: '.*:PURGE_NOHIT_MODELS:AGAT_SPFILTERFEATUREFROMKILLLIST' { ext.prefix = { "${meta.id}.nohits.purged" } } } diff --git a/nextflow.config b/nextflow.config index 60b2dc2..5e4e182 100644 --- a/nextflow.config +++ b/nextflow.config @@ -39,7 +39,7 @@ params { eggnogmapper_tax_scope = null eggnogmapper_evalue = 0.00001 eggnogmapper_pident = 35 - eggnogmapper_purge_nohits = true + eggnogmapper_purge_nohits = false // Max job request options max_cpus = 12 diff --git a/nextflow_schema.json b/nextflow_schema.json index b1c5fd9..747ff39 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -209,7 +209,7 @@ "eggnogmapper_purge_nohits": { "type": "boolean", "default": true, - "description": "Purge predicted transcripts which do not have a hit against eggnog" + "description": "Purge transcripts which do not have a hit against eggnog" } } }, diff --git a/subworkflows/local/purge_nohit_braker_models.nf b/subworkflows/local/purge_nohit_models.nf similarity index 88% rename from subworkflows/local/purge_nohit_braker_models.nf rename to subworkflows/local/purge_nohit_models.nf index 038380d..11230cf 100644 --- a/subworkflows/local/purge_nohit_braker_models.nf +++ b/subworkflows/local/purge_nohit_models.nf @@ -1,16 +1,16 @@ include { AGAT_SPFILTERFEATUREFROMKILLLIST } from '../../modules/pfr/agat/spfilterfeaturefromkilllist/main' -workflow PURGE_NOHIT_BRAKER_MODELS { +workflow PURGE_NOHIT_MODELS { take: - ch_braker_gff // [ meta, gff ] - ch_eggnogmapper_hits // [ meta, hits ] - val_purge_nohits // val(true|false) + ch_target_gff // [ meta, gff ] + ch_eggnogmapper_hits // [ meta, hits ] + val_purge_nohits // val(true|false) main: - ch_versions = Channel.empty() + ch_versions = Channel.empty() // COLLECTFILE: Transcript level kill list - ch_kill_list = ch_braker_gff + ch_kill_list = ch_target_gff | join(ch_eggnogmapper_hits) | map { meta, gff, hits -> @@ -45,7 +45,7 @@ workflow PURGE_NOHIT_BRAKER_MODELS { // MODULE: AGAT_SPFILTERFEATUREFROMKILLLIST ch_agat_kill_inputs = ! val_purge_nohits ? Channel.empty() - : ch_braker_gff + : ch_target_gff | join(ch_kill_list) @@ -55,13 +55,13 @@ workflow PURGE_NOHIT_BRAKER_MODELS { [] // default config ) - ch_braker_purged_gff = AGAT_SPFILTERFEATUREFROMKILLLIST.out.gff + ch_target_purged_gff = AGAT_SPFILTERFEATUREFROMKILLLIST.out.gff ch_versions = ch_versions.mix(AGAT_SPFILTERFEATUREFROMKILLLIST.out.versions.first()) // COLLECTFILE: Mark transcripts with note=No%20eggnog%20hit ch_marked_gff = val_purge_nohits ? Channel.empty() - : ch_braker_gff + : ch_target_gff | join(ch_kill_list) | map { meta, gff, lst -> def tx_without_hits = lst.readLines().collect { "$it".trim() } @@ -93,6 +93,6 @@ workflow PURGE_NOHIT_BRAKER_MODELS { } emit: - purged_or_marked_gff = ch_braker_purged_gff.mix(ch_marked_gff) + purged_or_marked_gff = ch_target_purged_gff.mix(ch_marked_gff) versions = ch_versions // [ versions.yml ] } diff --git a/workflows/pangene.nf b/workflows/pangene.nf index b2f8a7a..839a94b 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -7,8 +7,9 @@ include { PREPARE_EXT_PROTS } from '../subworkflows/local/pr include { FASTA_BRAKER3 } from '../subworkflows/local/fasta_braker3' include { FASTA_LIFTOFF } from '../subworkflows/local/fasta_liftoff' include { PURGE_BREAKER_MODELS } from '../subworkflows/local/purge_breaker_models' +include { AGAT_SPMERGEANNOTATIONS } from '../modules/pfr/agat/spmergeannotations/main' include { GFF_EGGNOGMAPPER } from '../subworkflows/local/gff_eggnogmapper' -include { PURGE_NOHIT_BRAKER_MODELS } from '../subworkflows/local/purge_nohit_braker_models' +include { PURGE_NOHIT_MODELS } from '../subworkflows/local/purge_nohit_models' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions' log.info paramsSummaryLog(workflow) @@ -214,25 +215,44 @@ workflow PANGENE { ch_braker_purged_gff = PURGE_BREAKER_MODELS.out.braker_purged_gff ch_versions = ch_versions.mix(PURGE_BREAKER_MODELS.out.versions) - // // SUBWORKFLOW: GFF_EGGNOGMAPPER - // GFF_EGGNOGMAPPER( - // ch_braker_purged_gff, - // ch_valid_target_assembly, - // params.eggnogmapper_db_dir, - // ) - - // ch_eggnogmapper_hits = GFF_EGGNOGMAPPER.out.eggnogmapper_hits - // ch_versions = ch_versions.mix(GFF_EGGNOGMAPPER.out.versions) - - // // SUBWORKFLOW: PURGE_NOHIT_BRAKER_MODELS - // PURGE_NOHIT_BRAKER_MODELS( - // ch_braker_purged_gff, - // ch_eggnogmapper_hits, - // params.eggnogmapper_purge_nohits - // ) - - // ch_braker_purged_marked_gff = PURGE_NOHIT_BRAKER_MODELS.out.purged_or_marked_gff - // ch_versions = ch_versions.mix(PURGE_NOHIT_BRAKER_MODELS.out.versions) + // MODULE: AGAT_SPMERGEANNOTATIONS + ch_gff_branch = ch_liftoff_gff3 + | join(ch_braker_purged_gff, remainder:true) + | branch { meta, liftoff_gff, braker_gff -> + both : ( liftoff_gff && braker_gff ) + liftoff_only: ( liftoff_gff && ( ! braker_gff ) ) + braker_only : ( ( ! liftoff_gff ) && braker_gff ) + } + + AGAT_SPMERGEANNOTATIONS( + ch_gff_branch.both.map { meta, lg, bg -> [ meta, [ lg, bg ] ] }, + [] + ) + + ch_merged_gff = AGAT_SPMERGEANNOTATIONS.out.gff + | mix(ch_gff_branch.liftoff_only) + | mix(ch_gff_branch.braker_only) + ch_versions = ch_versions.mix(AGAT_SPMERGEANNOTATIONS.out.versions.first()) + + // SUBWORKFLOW: GFF_EGGNOGMAPPER + GFF_EGGNOGMAPPER( + ch_merged_gff, + ch_valid_target_assembly, + params.eggnogmapper_db_dir, + ) + + ch_eggnogmapper_hits = GFF_EGGNOGMAPPER.out.eggnogmapper_hits + ch_versions = ch_versions.mix(GFF_EGGNOGMAPPER.out.versions) + + // SUBWORKFLOW: PURGE_NOHIT_MODELS + PURGE_NOHIT_MODELS( + ch_merged_gff, + ch_eggnogmapper_hits, + params.eggnogmapper_purge_nohits + ) + + ch_purged_marked_gff = PURGE_NOHIT_MODELS.out.purged_or_marked_gff + ch_versions = ch_versions.mix(PURGE_NOHIT_MODELS.out.versions) // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( From 70898ca146d1a3fcad044c066f2259386792287b Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Thu, 18 Apr 2024 13:22:42 +1200 Subject: [PATCH 27/40] Added GFF_MERGE_CLEANUP sub-workflow --- CHANGELOG.md | 1 + conf/modules.config | 6 +- subworkflows/local/gff_merge_cleanup.nf | 115 +++++++++++++++++++++ subworkflows/local/purge_breaker_models.nf | 4 +- subworkflows/local/purge_nohit_models.nf | 2 +- workflows/pangene.nf | 24 ++--- 6 files changed, 131 insertions(+), 21 deletions(-) create mode 100644 subworkflows/local/gff_merge_cleanup.nf diff --git a/CHANGELOG.md b/CHANGELOG.md index 90e24b2..35ca6fe 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -37,6 +37,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 28. Added `PURGE_NOHIT_BRAKER_MODELS` sub-workflow 29. Removed liftoff models with `valid_ORF=False` 30. Now merging BRAKER and liftoff models before running eggnogmapper +31. Added `GFF_MERGE_CLEANUP` sub-workflow ### `Fixed` diff --git a/conf/modules.config b/conf/modules.config index 9824dda..215847d 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -197,9 +197,13 @@ process { } process { - withName: '.*:PANGENE:AGAT_SPMERGEANNOTATIONS' { + withName: '.*:GFF_MERGE_CLEANUP:AGAT_SPMERGEANNOTATIONS' { ext.prefix = { "${meta.id}.liftoff.braker" } } + + withName: '.*:GFF_MERGE_CLEANUP:GT_GFF3' { + ext.args = '-tidy -retainids' + } } process { diff --git a/subworkflows/local/gff_merge_cleanup.nf b/subworkflows/local/gff_merge_cleanup.nf new file mode 100644 index 0000000..be02209 --- /dev/null +++ b/subworkflows/local/gff_merge_cleanup.nf @@ -0,0 +1,115 @@ +include { AGAT_SPMERGEANNOTATIONS } from '../../modules/pfr/agat/spmergeannotations/main' +include { GT_GFF3 } from '../../modules/nf-core/gt/gff3/main' +include { AGAT_CONVERTSPGXF2GXF } from '../../modules/nf-core/agat/convertspgxf2gxf/main' + +workflow GFF_MERGE_CLEANUP { + take: + ch_braker_gff // Channel: [ meta, gff ] + ch_liftoff_gff // Channel: [ meta, gff ] + + main: + ch_versions = Channel.empty() + + ch_gff_branch = ch_braker_gff + | join(ch_liftoff_gff, remainder:true) + | branch { meta, braker_gff, liftoff_gff -> + both : ( braker_gff && liftoff_gff ) + braker_only : ( braker_gff && ( ! liftoff_gff ) ) + liftoff_only: ( ( ! braker_gff ) && liftoff_gff ) + } + + // MODULE: AGAT_SPMERGEANNOTATIONS + AGAT_SPMERGEANNOTATIONS( + ch_gff_branch.both.map { meta, bg, lg -> [ meta, [ bg, lg ] ] }, + [] + ) + + ch_merged_gff = AGAT_SPMERGEANNOTATIONS.out.gff + | mix(ch_gff_branch.liftoff_only) + | mix(ch_gff_branch.braker_only) + ch_versions = ch_versions.mix(AGAT_SPMERGEANNOTATIONS.out.versions.first()) + + // MODULE: GT_GFF3 + GT_GFF3 ( ch_merged_gff ) + + ch_gt_gff = GT_GFF3.out.gt_gff3 + ch_versions = ch_versions.mix(GT_GFF3.out.versions.first()) + + // COLLECTFILE: Format GT_GFF3 output + ch_gt_formatted_gff = ch_gt_gff + | map { meta, gff -> + + def lines = gff.readLines() + .collect { line -> + if ( line.startsWith('##') ) { return line } + if ( line.startsWith('#') ) { return '' } + + def cols = line.split('\t') + def program = cols[1] + def feat = cols[2] + def atts = cols[8] + + def atts_r = '' + // Remove attributes and use AGAT_CONVERTSPGXF2GXF + // to create attributes based on sequential layout + + if ( feat != 'gene' || program != 'Liftoff' ) { + return ( cols[0..7] + [ atts_r ] ).join('\t') + } + + def gene_id = ( atts =~ /ID=([^;]*)/ )[0][1] + def atts_g = "liftoffID=$gene_id" + + return ( cols[0..7] + [ atts_g ] ).join('\t') + + }.join('\n') + + [ "${meta.id}.bare.gff" ] + [ lines ] + } + | collectFile(newLine: true) + | map { file -> + [ [ id: file.baseName.replace('.bare', '') ], file ] + } + + // MODULE: AGAT_CONVERTSPGXF2GXF + AGAT_CONVERTSPGXF2GXF ( ch_gt_formatted_gff ) + + ch_agat_gff = AGAT_CONVERTSPGXF2GXF.out.output_gff + ch_versions = ch_versions.mix(AGAT_CONVERTSPGXF2GXF.out.versions.first()) + + // COLLECTFILE: Format AGAT_CONVERTSPGXF2GXF output + ch_final_gff = ch_agat_gff + | map { meta, gff -> + + def lines = gff.readLines() + .collect { line -> + if ( line.startsWith('#') ) { return line } + + def cols = line.split('\t') + def program = cols[1] + def feat = cols[2] + def atts = cols[8] + def atts_r = atts.replace('-', '').replace('agat', '') + + if ( feat != 'gene' || program != 'Liftoff' ) { + return ( cols[0..7] + [ atts_r ] ).join('\t') + } + + def oldID = ( atts =~ /liftoffID=([^;]*)/ )[0][1] + def newID = ( atts =~ /ID=([^;]*)/ )[0][1].replace('-', '').replace('agat', '') + def atts_g = "ID=${newID};liftoffID=${oldID}" + + return ( cols[0..7] + [ atts_g ] ).join('\t') + }.join('\n') + + [ "${meta.id}.agat.cleanup.gff" ] + [ lines ] + } + | collectFile(newLine: true) + | map { file -> + [ [ id: file.baseName.replace('.agat.cleanup', '') ], file ] + } + + emit: + gff = ch_final_gff // [ meta, gff ] + versions = ch_versions // [ versions.yml ] +} diff --git a/subworkflows/local/purge_breaker_models.nf b/subworkflows/local/purge_breaker_models.nf index 2f00cf5..6633246 100644 --- a/subworkflows/local/purge_breaker_models.nf +++ b/subworkflows/local/purge_breaker_models.nf @@ -96,9 +96,9 @@ workflow PURGE_BREAKER_MODELS { // COLLECTFILE: Format AGAT_CONVERTSPGXF2GXF output ch_tsebra_gff = ch_tsebra_formatted_gff - | map { meta, gtf -> + | map { meta, gff -> - def lines = gtf.readLines() + def lines = gff.readLines() .collect { line -> if ( line.startsWith('#') ) { return line } diff --git a/subworkflows/local/purge_nohit_models.nf b/subworkflows/local/purge_nohit_models.nf index 11230cf..b83f764 100644 --- a/subworkflows/local/purge_nohit_models.nf +++ b/subworkflows/local/purge_nohit_models.nf @@ -85,7 +85,7 @@ workflow PURGE_NOHIT_MODELS { return ( line + ';note=No%20eggnog%20hit' ) } - [ "${meta.id}.marked.gff3" ] + marked_gff_lines.join('\n') + [ "${meta.id}.marked.gff" ] + marked_gff_lines.join('\n') } | collectFile(newLine: true) | map { file -> diff --git a/workflows/pangene.nf b/workflows/pangene.nf index 839a94b..3712a34 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -7,7 +7,7 @@ include { PREPARE_EXT_PROTS } from '../subworkflows/local/pr include { FASTA_BRAKER3 } from '../subworkflows/local/fasta_braker3' include { FASTA_LIFTOFF } from '../subworkflows/local/fasta_liftoff' include { PURGE_BREAKER_MODELS } from '../subworkflows/local/purge_breaker_models' -include { AGAT_SPMERGEANNOTATIONS } from '../modules/pfr/agat/spmergeannotations/main' +include { GFF_MERGE_CLEANUP } from '../subworkflows/local/gff_merge_cleanup' include { GFF_EGGNOGMAPPER } from '../subworkflows/local/gff_eggnogmapper' include { PURGE_NOHIT_MODELS } from '../subworkflows/local/purge_nohit_models' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions' @@ -215,24 +215,14 @@ workflow PANGENE { ch_braker_purged_gff = PURGE_BREAKER_MODELS.out.braker_purged_gff ch_versions = ch_versions.mix(PURGE_BREAKER_MODELS.out.versions) - // MODULE: AGAT_SPMERGEANNOTATIONS - ch_gff_branch = ch_liftoff_gff3 - | join(ch_braker_purged_gff, remainder:true) - | branch { meta, liftoff_gff, braker_gff -> - both : ( liftoff_gff && braker_gff ) - liftoff_only: ( liftoff_gff && ( ! braker_gff ) ) - braker_only : ( ( ! liftoff_gff ) && braker_gff ) - } - - AGAT_SPMERGEANNOTATIONS( - ch_gff_branch.both.map { meta, lg, bg -> [ meta, [ lg, bg ] ] }, - [] + // SUBWORKFLOW: GFF_MERGE_CLEANUP + GFF_MERGE_CLEANUP( + ch_braker_purged_gff, + ch_liftoff_gff3 ) - ch_merged_gff = AGAT_SPMERGEANNOTATIONS.out.gff - | mix(ch_gff_branch.liftoff_only) - | mix(ch_gff_branch.braker_only) - ch_versions = ch_versions.mix(AGAT_SPMERGEANNOTATIONS.out.versions.first()) + ch_merged_gff = GFF_MERGE_CLEANUP.out.gff + ch_versions = ch_versions.mix(GFF_MERGE_CLEANUP.out.versions) // SUBWORKFLOW: GFF_EGGNOGMAPPER GFF_EGGNOGMAPPER( From 8fc09585cc49bdca368c710271b1c95eda1a0957 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 22 Apr 2024 14:10:50 +1200 Subject: [PATCH 28/40] Now using description field to store notes and textual annotations in the gff files --- CHANGELOG.md | 6 +++++- nextflow_schema.json | 2 +- subworkflows/local/gff_merge_cleanup.nf | 5 ++++- subworkflows/local/purge_nohit_models.nf | 4 ++-- 4 files changed, 12 insertions(+), 5 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 35ca6fe..cfcb217 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 0.2.0+dev - [18-April-2024] +## 0.2.0+dev - [22-April-2024] ### `Added` @@ -38,11 +38,15 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 29. Removed liftoff models with `valid_ORF=False` 30. Now merging BRAKER and liftoff models before running eggnogmapper 31. Added `GFF_MERGE_CLEANUP` sub-workflow +32. Now using `description` field to store notes and textual annotations in the gff files +33. Now using `mRNA` in place of `transcript` in gff files +34. Now `eggnogmapper_purge_nohits` is set to `false` by default ### `Fixed` ### `Dependencies` 1. NextFlow!>=23.04.4 +2. nf-validation=1.1.3 ### `Deprecated` diff --git a/nextflow_schema.json b/nextflow_schema.json index 747ff39..f1081eb 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -208,7 +208,7 @@ }, "eggnogmapper_purge_nohits": { "type": "boolean", - "default": true, + "default": false, "description": "Purge transcripts which do not have a hit against eggnog" } } diff --git a/subworkflows/local/gff_merge_cleanup.nf b/subworkflows/local/gff_merge_cleanup.nf index be02209..286ea45 100644 --- a/subworkflows/local/gff_merge_cleanup.nf +++ b/subworkflows/local/gff_merge_cleanup.nf @@ -53,8 +53,11 @@ workflow GFF_MERGE_CLEANUP { // Remove attributes and use AGAT_CONVERTSPGXF2GXF // to create attributes based on sequential layout + def feat_r = feat == 'transcript' ? 'mRNA' : feat + // Use mRNA inplace of transcript + if ( feat != 'gene' || program != 'Liftoff' ) { - return ( cols[0..7] + [ atts_r ] ).join('\t') + return ( cols[0..1] + [ feat_r ] + cols[3..7] + [ atts_r ] ).join('\t') } def gene_id = ( atts =~ /ID=([^;]*)/ )[0][1] diff --git a/subworkflows/local/purge_nohit_models.nf b/subworkflows/local/purge_nohit_models.nf index b83f764..b75cb07 100644 --- a/subworkflows/local/purge_nohit_models.nf +++ b/subworkflows/local/purge_nohit_models.nf @@ -58,7 +58,7 @@ workflow PURGE_NOHIT_MODELS { ch_target_purged_gff = AGAT_SPFILTERFEATUREFROMKILLLIST.out.gff ch_versions = ch_versions.mix(AGAT_SPFILTERFEATUREFROMKILLLIST.out.versions.first()) - // COLLECTFILE: Mark transcripts with note=No%20eggnog%20hit + // COLLECTFILE: Mark transcripts with description=hypothetical%20protein%20%7C%20no%20eggnog%20hit ch_marked_gff = val_purge_nohits ? Channel.empty() : ch_target_gff @@ -82,7 +82,7 @@ workflow PURGE_NOHIT_MODELS { if ( ! ( tx_id in tx_without_hits ) ) { return line } - return ( line + ';note=No%20eggnog%20hit' ) + return ( line + ';description=hypothetical%20protein%20%7C%20no%20eggnog%20hit' ) } [ "${meta.id}.marked.gff" ] + marked_gff_lines.join('\n') From 57138fdbcb6e40ed28ad785e8cd2f4dbd673c06a Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 24 Apr 2024 09:59:22 +1200 Subject: [PATCH 29/40] Added GFF_STORE sub workflow --- CHANGELOG.md | 1 + conf/modules.config | 20 +++- subworkflows/local/gff_store.nf | 114 +++++++++++++++++++++++ subworkflows/local/purge_nohit_models.nf | 36 +------ workflows/pangene.nf | 10 +- 5 files changed, 144 insertions(+), 37 deletions(-) create mode 100644 subworkflows/local/gff_store.nf diff --git a/CHANGELOG.md b/CHANGELOG.md index cfcb217..952ea21 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -41,6 +41,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 32. Now using `description` field to store notes and textual annotations in the gff files 33. Now using `mRNA` in place of `transcript` in gff files 34. Now `eggnogmapper_purge_nohits` is set to `false` by default +35. Added `GFF_STORE` sub workflow ### `Fixed` diff --git a/conf/modules.config b/conf/modules.config index 215847d..66163de 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -202,7 +202,7 @@ process { } withName: '.*:GFF_MERGE_CLEANUP:GT_GFF3' { - ext.args = '-tidy -retainids' + ext.args = '-tidy -retainids -sort' } } @@ -220,6 +220,12 @@ process { "--itype proteins", '--go_evidence all' ].join(' ').trim() + + publishDir = [ + path: { "${params.outdir}/final/$meta.id" }, + mode: "copy", + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + ] } } @@ -229,6 +235,18 @@ process { } } +process { + withName: 'FINAL_GFF_CHECK' { + ext.args = '-tidy -retainids -sort' + + publishDir = [ + path: { "${params.outdir}/final/$meta.id" }, + mode: "copy", + saveAs: { filename -> filename.equals('versions.yml') ? null : filename }, + ] + } +} + process { withName: 'CUSTOM_DUMPSOFTWAREVERSIONS' { publishDir = [ diff --git a/subworkflows/local/gff_store.nf b/subworkflows/local/gff_store.nf new file mode 100644 index 0000000..f487a50 --- /dev/null +++ b/subworkflows/local/gff_store.nf @@ -0,0 +1,114 @@ +import java.net.URLEncoder + +include { GT_GFF3 as FINAL_GFF_CHECK } from '../../modules/nf-core/gt/gff3/main' + +workflow GFF_STORE { + take: + ch_target_gff // [ meta, gff ] + ch_eggnogmapper_annotations // [ meta, annotations ] + + main: + ch_versions = Channel.empty() + + // COLLECTFILE: Add eggnogmapper hits to gff + ch_described_gff = ch_target_gff + | join(ch_eggnogmapper_annotations) + | map { meta, gff, annotations -> + def tx_annotations = annotations.readLines() + .findAll { ! it.startsWith('#') } + .collect { line -> + def cols = line.split('\t') + def id = cols[0] + def txt = cols[7] + + [ id, txt ] + } + .findAll { id, txt -> + txt != '-' + }.collectEntries { id, txt -> + [ id, txt ] + } + + def gene_tx_annotations = [:] + gff.readLines() + .findAll { line -> + if ( line.startsWith('#') ) { return false } + + def cols = line.split('\t') + def feat = cols[2] + + if ( ! ( feat == 'transcript' || feat == 'mRNA' ) ) { return false } + + return true + } + .each { line -> + def cols = line.split('\t') + def atts = cols[8] + + def matches = atts =~ /ID=([^;]*)/ + def tx_id = matches[0][1] + + def matches_p= atts =~ /Parent=([^;]*)/ + def gene_id = matches_p[0][1] + + if ( ! gene_tx_annotations.containsKey(gene_id) ) { + gene_tx_annotations[gene_id] = [:] + } + + def anno = tx_annotations.containsKey(tx_id) + ? URLEncoder.encode(tx_annotations[tx_id], "UTF-8").replace('+', '%20') + : URLEncoder.encode('hypothetical protein | no eggnog hit', "UTF-8").replace('+', '%20') + + gene_tx_annotations[gene_id] += [ ( tx_id ): anno ] + } + + gene_tx_annotations = gene_tx_annotations + .collectEntries { gene_id, tx_annos -> + def default_anno = tx_annos.values().first() + + if ( tx_annos.values().findAll { it != default_anno }.size() > 0 ) { + return [ gene_id, ( tx_annos + [ 'default': 'differing%20isoform%20descriptions' ] ) ] + } + + [ gene_id, ( tx_annos + [ 'default': default_anno ] ) ] + } + + def gff_lines = gff.readLines() + .collect { line -> + + if ( line.startsWith('#') ) { return line } + + def cols = line.split('\t') + def feat = cols[2] + def atts = cols[8] + + if ( ! ( feat == 'gene' || feat == 'transcript' || feat == 'mRNA' ) ) { return line } + + def id = feat == 'gene' ? ( atts =~ /ID=([^;]*)/ )[0][1] : ( atts =~ /Parent=([^;]*)/ )[0][1] + + if ( ! gene_tx_annotations.containsKey(id) ) { return line } + + def tx_id = feat == 'gene' ? null : ( atts =~ /ID=([^;]*)/ )[0][1] + def desc = feat == 'gene' ? gene_tx_annotations[id]['default'] : gene_tx_annotations[id][tx_id] + + return ( line + ";description=$desc" ) + } + + [ "${meta.id}.described.gff" ] + gff_lines.join('\n') + } + | collectFile(newLine: true) + | map { file -> + [ [ id: file.baseName.replace('.described', '') ], file ] + } + + // MODULE: GT_GFF3 as FINAL_GFF_CHECK + FINAL_GFF_CHECK ( ch_described_gff ) + + ch_final_gff = FINAL_GFF_CHECK.out.gt_gff3 + ch_versions = ch_versions.mix(FINAL_GFF_CHECK.out.versions.first()) + + + emit: + final_gff = ch_final_gff // [ meta, gff ] + versions = ch_versions // [ versions.yml ] +} diff --git a/subworkflows/local/purge_nohit_models.nf b/subworkflows/local/purge_nohit_models.nf index b75cb07..5c22f5f 100644 --- a/subworkflows/local/purge_nohit_models.nf +++ b/subworkflows/local/purge_nohit_models.nf @@ -58,41 +58,7 @@ workflow PURGE_NOHIT_MODELS { ch_target_purged_gff = AGAT_SPFILTERFEATUREFROMKILLLIST.out.gff ch_versions = ch_versions.mix(AGAT_SPFILTERFEATUREFROMKILLLIST.out.versions.first()) - // COLLECTFILE: Mark transcripts with description=hypothetical%20protein%20%7C%20no%20eggnog%20hit - ch_marked_gff = val_purge_nohits - ? Channel.empty() - : ch_target_gff - | join(ch_kill_list) - | map { meta, gff, lst -> - def tx_without_hits = lst.readLines().collect { "$it".trim() } - - def marked_gff_lines = gff.readLines() - .collect { line -> - - if ( line.startsWith('#') ) { return line } - - def cols = line.split('\t') - def feat = cols[2] - - if ( ! ( feat == 'transcript' || feat == 'mRNA' ) ) { return line } - - def atts = cols[8] - def matches = atts =~ /ID=([^;]*)/ - def tx_id = matches[0][1] - - if ( ! ( tx_id in tx_without_hits ) ) { return line } - - return ( line + ';description=hypothetical%20protein%20%7C%20no%20eggnog%20hit' ) - } - - [ "${meta.id}.marked.gff" ] + marked_gff_lines.join('\n') - } - | collectFile(newLine: true) - | map { file -> - [ [ id: file.baseName.replace('.marked', '') ], file ] - } - emit: - purged_or_marked_gff = ch_target_purged_gff.mix(ch_marked_gff) + purged_gff = ch_target_purged_gff.mix(val_purge_nohits ? Channel.empty() : ch_target_gff) versions = ch_versions // [ versions.yml ] } diff --git a/workflows/pangene.nf b/workflows/pangene.nf index 3712a34..66a0459 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -10,6 +10,7 @@ include { PURGE_BREAKER_MODELS } from '../subworkflows/local/pu include { GFF_MERGE_CLEANUP } from '../subworkflows/local/gff_merge_cleanup' include { GFF_EGGNOGMAPPER } from '../subworkflows/local/gff_eggnogmapper' include { PURGE_NOHIT_MODELS } from '../subworkflows/local/purge_nohit_models' +include { GFF_STORE } from '../subworkflows/local/gff_store' include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions' log.info paramsSummaryLog(workflow) @@ -232,6 +233,7 @@ workflow PANGENE { ) ch_eggnogmapper_hits = GFF_EGGNOGMAPPER.out.eggnogmapper_hits + ch_eggnogmapper_annotations = GFF_EGGNOGMAPPER.out.eggnogmapper_annotations ch_versions = ch_versions.mix(GFF_EGGNOGMAPPER.out.versions) // SUBWORKFLOW: PURGE_NOHIT_MODELS @@ -241,9 +243,15 @@ workflow PANGENE { params.eggnogmapper_purge_nohits ) - ch_purged_marked_gff = PURGE_NOHIT_MODELS.out.purged_or_marked_gff + ch_purged_gff = PURGE_NOHIT_MODELS.out.purged_gff ch_versions = ch_versions.mix(PURGE_NOHIT_MODELS.out.versions) + // SUBWORKFLOW: GFF_STORE + GFF_STORE( + ch_purged_gff, + ch_eggnogmapper_annotations + ) + // MODULE: CUSTOM_DUMPSOFTWAREVERSIONS CUSTOM_DUMPSOFTWAREVERSIONS ( ch_versions.unique().collectFile(name: 'collated_versions.yml') From fe6578f901a8d95b9127fc84d639c83c8138e8a7 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 24 Apr 2024 12:44:53 +1200 Subject: [PATCH 30/40] Now using local tests/stub files for GitHub CI --- .github/workflows/test.yml | 11 +-- CHANGELOG.md | 23 ++++--- LICENSE | 2 +- README.md | 58 ++++++++++++---- conf/test_params.json | 11 --- conf/test_stub.config | 10 --- docs/contributors.sh | 5 ++ docs/parameters.md | 68 +++++++++++++++++++ local_pangene | 4 +- modules/kherronism/braker3/main.nf | 3 + nextflow.config | 10 +-- nextflow_schema.json | 41 ++++++----- pfr/profile.config | 17 +++++ pfr_pangene | 37 ++++++++-- subworkflows/local/gff_eggnogmapper.nf | 2 +- subworkflows/local/gff_merge_cleanup.nf | 4 +- subworkflows/local/gff_store.nf | 4 +- subworkflows/local/purge_nohit_models.nf | 7 +- .../stub/emapperdb/5.0.2/eggnog.db | 0 .../stub/emapperdb/5.0.2/eggnog_proteins.dmnd | 0 tests/stub/external-protein-fastas.txt | 4 +- tests/stub/liftoffannotations.csv | 4 +- tests/stub/test_stub.json | 9 +++ workflows/pangene.nf | 9 ++- 24 files changed, 245 insertions(+), 98 deletions(-) delete mode 100644 conf/test_params.json delete mode 100644 conf/test_stub.config create mode 100755 docs/contributors.sh create mode 100644 docs/parameters.md create mode 100644 pfr/profile.config rename TODO.md => tests/stub/emapperdb/5.0.2/eggnog.db (100%) create mode 100644 tests/stub/emapperdb/5.0.2/eggnog_proteins.dmnd create mode 100644 tests/stub/test_stub.json diff --git a/.github/workflows/test.yml b/.github/workflows/test.yml index 65c2fa1..da21d2f 100644 --- a/.github/workflows/test.yml +++ b/.github/workflows/test.yml @@ -39,12 +39,6 @@ jobs: steps: - uses: actions/checkout@v4 - - uses: actions/checkout@v4 - with: - repository: PlantandFoodResearch/pangene-test - ssh-key: ${{ secrets.PANGENE_TEST_DEPLOY_KEY }} - path: pangene-test - - name: Install Nextflow uses: nf-core/setup-nextflow@v1 with: @@ -52,12 +46,11 @@ jobs: - name: Run stub-test run: | - nextflow \ + nextflow run \ main.nf \ -profile local,docker \ - -resume \ -stub \ - -c conf/test_stub.config + -params-file tests/stub/test_stub.json confirm-pass: runs-on: ubuntu-latest diff --git a/CHANGELOG.md b/CHANGELOG.md index 952ea21..7234e70 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 0.2.0+dev - [22-April-2024] +## 0.2.0 - [24-April-2024] ### `Added` @@ -35,16 +35,23 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 26. Now using TSEBRA to purge models which do not have full intron support from BRAKER hints 27. Added params `eggnogmapper_evalue` and `eggnogmapper_pident` 28. Added `PURGE_NOHIT_BRAKER_MODELS` sub-workflow -29. Removed liftoff models with `valid_ORF=False` -30. Now merging BRAKER and liftoff models before running eggnogmapper -31. Added `GFF_MERGE_CLEANUP` sub-workflow -32. Now using `description` field to store notes and textual annotations in the gff files -33. Now using `mRNA` in place of `transcript` in gff files -34. Now `eggnogmapper_purge_nohits` is set to `false` by default -35. Added `GFF_STORE` sub workflow +29. Now merging BRAKER and liftoff models before running eggnogmapper +30. Added `GFF_MERGE_CLEANUP` sub-workflow +31. Now using `description` field to store notes and textual annotations in the gff files +32. Now using `mRNA` in place of `transcript` in gff files +33. Now `eggnogmapper_purge_nohits` is set to `false` by default +34. Added `GFF_STORE` sub workflow +35. `external_protein_fastas` and `eggnogmapper_db_dir` are not mandatory parameters +36. Added contributors +37. Add a document for the pipeline parameters +38. Updated `pfr_pangene` and `pfr/profile.config` +39. Now using local tests/stub files for GitHub CI ### `Fixed` +1. Removed liftoff models with `valid_ORF=False` +2. Updated license text to include 'Copyright (c) 2024 The New Zealand Institute for Plant and Food Research Limited' + ### `Dependencies` 1. NextFlow!>=23.04.4 diff --git a/LICENSE b/LICENSE index 9106c5a..2ef2204 100644 --- a/LICENSE +++ b/LICENSE @@ -1,6 +1,6 @@ MIT License -Copyright (c) Usman Rashid, Jason Shiller +Copyright (c) 2024 The New Zealand Institute for Plant and Food Research Limited Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal diff --git a/README.md b/README.md index 2016044..65d08cf 100644 --- a/README.md +++ b/README.md @@ -2,7 +2,7 @@ [![Lint and -stub on Linux/Docker](https://github.com/PlantandFoodResearch/pangene/actions/workflows/test.yml/badge.svg)](https://github.com/PlantandFoodResearch/pangene/actions/workflows/test.yml) -A NextFlow pipeline for pan-genome annotation. +A NextFlow pipeline for pan-genome annotation. It can also be used for annotation of a single genome. ## Pipeline Flowchart @@ -84,26 +84,60 @@ flowchart TD style Annotation fill:#00FFFF21,stroke:#00FFFF21 ``` +## Alpha Release + +This release is not fully documented and under alpha testing by the Bioinformatics Team. There are several [outstanding issues](https://github.com/PlantandFoodResearch/pangene/issues) which will be addressed before a general release. + ## Plant&Food Users -Configure the pipeline by modifying `nextflow.config` and submit to SLURM for execution. +Download the pipeline to your `/workspace/$USER` folder. Change the parameters defined in the [pfr/params.json](./pfr/params.json) file. Submit the pipeline to SLURM for execution. For a description of the parameters, see [parameters](./docs/parameters.md). ```bash -sbatch ./pangene_pfr +sbatch ./pfr_pangene ``` -## Third-party Sources - -Some software components of this pipeline have been adopted from following third-party sources: - -1. nf-core [MIT](https://github.com/nf-core/modules/blob/master/LICENSE): https://github.com/nf-core/modules +## Credits + +plantandfoodresearch/pangene scripts were originally written by Jason Shiller. Usman Rashid wrote the NextFLow pipeline. + +We thank the following people for their extensive assistance in the development of this pipeline. + +- Cecilia Deng [@CeciliaDeng](https://github.com/CeciliaDeng) +- Charles David [@charlesdavid](https://github.com/charlesdavid) +- Chen Wu [@christinawu2008](https://github.com/christinawu2008) +- Leonardo Salgado [@leorippel](https://github.com/leorippel) +- Ross Crowhurst [@rosscrowhurst](https://github.com/rosscrowhurst) +- Susan Thomson [@cflsjt](https://github.com/cflsjt) +- Ting-Hsuan Chen [@ting-hsuan-chen](https://github.com/ting-hsuan-chen) + +The pipeline uses nf-core modules contributed by following authors. + + + + + + + + + + + + + + + + + + + + + +## Citations + +This pipeline uses code and infrastructure developed and maintained by the [nf-core](https://nf-co.re) community, reused here under the [MIT license](https://github.com/nf-core/tools/blob/master/LICENSE). > **The nf-core framework for community-curated bioinformatics pipelines.** > > Philip Ewels, Alexander Peltzer, Sven Fillinger, Harshil Patel, Johannes Alneberg, Andreas Wilm, Maxime Ulysse Garcia, Paolo Di Tommaso & Sven Nahnsen. > > _Nat Biotechnol._ 2020 Feb 13. doi: [10.1038/s41587-020-0439-x](https://dx.doi.org/10.1038/s41587-020-0439-x). - -2. nf-core/rnaseq [MIT](https://github.com/nf-core/rnaseq/blob/master/LICENSE): https://github.com/nf-core/rnaseq -3. rewarewaannotation [MIT](https://github.com/kherronism/rewarewaannotation/blob/master/LICENSE): https://github.com/kherronism/rewarewaannotation -4. assembly_qc [GPL-3.0](https://github.com/Plant-Food-Research-Open/assembly_qc/blob/main/LICENSE): https://github.com/Plant-Food-Research-Open/assembly_qc diff --git a/conf/test_params.json b/conf/test_params.json deleted file mode 100644 index 609fe6a..0000000 --- a/conf/test_params.json +++ /dev/null @@ -1,11 +0,0 @@ -{ - "input": "pangene-test/assemblysheet.csv", - "fastq": "pangene-test/fastqsheet.csv", - "external_protein_fastas": "pangene-test/external-protein-fastas.txt", - "liftoff_annotations": "pangene-test/liftoffannotations.csv", - "remove_ribo_rna": true, - "ribo_database_manifest": "assets/rrna-db-test.txt", - "braker_extra_args": "--rounds=1", - "max_cpus": 8, - "max_memory": "32.GB" -} diff --git a/conf/test_stub.config b/conf/test_stub.config deleted file mode 100644 index 2ebb70a..0000000 --- a/conf/test_stub.config +++ /dev/null @@ -1,10 +0,0 @@ -params { - - input = "tests/stub/assemblysheet.csv" - fastq = "tests/stub/fastqsheet.csv" - external_protein_fastas = "pangene-test/external-protein-fastas.txt" - liftoff_xref_annotations = "tests/stub/liftoffannotations.csv" - - max_cpus = 2 - max_memory = '3.GB' -} diff --git a/docs/contributors.sh b/docs/contributors.sh new file mode 100755 index 0000000..8dbfc36 --- /dev/null +++ b/docs/contributors.sh @@ -0,0 +1,5 @@ +#!/usr/bin/env bash + +module_authors=$(find ./modules -name meta.yml | xargs -I {} grep -A20 'authors:' {} | grep '\- ' | tr -d '[-" ]' | tr '[:upper:]' '[:lower:]') +workflow_authors=$(find ./subworkflows -name meta.yml | xargs -I {} grep -A20 'authors:' {} | grep '\- ' | tr -d '[-" ]' | tr '[:upper:]' '[:lower:]') +echo -e "${module_authors}\n${workflow_authors}" | sort -V | uniq | sed -n 's|@\(.*\)||p' diff --git a/docs/parameters.md b/docs/parameters.md new file mode 100644 index 0000000..8403d49 --- /dev/null +++ b/docs/parameters.md @@ -0,0 +1,68 @@ +# plantandfoodresearch/pangene pipeline parameters + +A NextFlow pipeline for pan-genome annotation + +## Input/output options + +| Parameter | Description | Type | Default | Required | Hidden | +| ------------------------- | ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | --------- | -------- | ------ | +| `input` | Target assemblies listed in a CSV sheet
HelpFASTA and other associated files for target assemblies provided as a CSV sheet
| `string` | | True | | +| `external_protein_fastas` | External protein fastas listed in a text sheet
HelpA text file listing FASTA files to provide protein evidence for annotation
| `string` | | True | | +| `eggnogmapper_db_dir` | Eggnogmapper database directory | `string` | | True | | +| `eggnogmapper_tax_scope` | Eggnogmapper taxonomy scopre | `integer` | | True | | +| `fastq` | FASTQ samples listed in a CSV sheet
HelpFASTQ files for RNASeq samples corresponding to each target assembly provided in a CSV sheet
| `string` | | | | +| `liftoff_annotations` | Reference annotations listed in a CSV sheet
HelpFASTA and GFF3 files for reference annotations for liftoff listed in a CSV sheet
| `string` | | | | +| `outdir` | The output directory where the results will be saved
Help Use absolute paths to storage on Cloud infrastructure
| `string` | ./results | True | | + +## Repeat annotation options + +| Parameter | Description | Type | Default | Required | Hidden | +| --------------------------- | ------------------------------------------ | --------- | ------------- | -------- | ------ | +| `repeat_annotator` | 'edta' or 'repeatmodeler' | `string` | repeatmodeler | | | +| `save_annotated_te_lib` | Save annotated TE library or not? | `boolean` | | | | +| `edta_is_sensitive` | Use '--sensitive 1' flag with EDTA or not? | `boolean` | | | | +| `repeatmasker_save_outputs` | Save the repeat-masked genome or not? | `boolean` | | | | + +## RNASeq pre-processing options + +| Parameter | Description | Type | Default | Required | Hidden | +| ------------------------ | ------------------------------------------------------------------ | --------- | ----------------------------------------- | -------- | ------ | +| `skip_fastqc` | Skip FASTQC or not? | `boolean` | | | | +| `skip_fastp` | Skip trimming by FASTQP or not? | `boolean` | | | | +| `min_trimmed_reads` | Exclude a sample if its reads after trimming are below this number | `integer` | 10000 | | | +| `extra_fastp_args` | Extra FASTP arguments | `string` | | | | +| `save_trimmed` | Save FASTQ files after trimming or not? | `boolean` | | | | +| `remove_ribo_rna` | Remove Ribosomal RNA or not? | `boolean` | | | | +| `save_non_ribo_reads` | Save FASTQ files after Ribosomal RNA removal or not? | `boolean` | | | | +| `ribo_database_manifest` | Ribosomal RNA fastas listed in a text sheet | `string` | ${projectDir}/assets/rrna-db-defaults.txt | | | + +## RNAseq alignment options + +| Parameter | Description | Type | Default | Required | Hidden | +| ------------------------ | ------------------------------------------------- | --------- | ------- | -------- | ------ | +| `star_max_intron_length` | Maximum intron length for STAR alignment | `integer` | 16000 | | | +| `star_align_extra_args` | EXTRA arguments for STAR | `string` | | | | +| `star_save_outputs` | Save BAM files from STAR or not? | `boolean` | | | | +| `save_cat_bam` | SAVE a concatenated BAM file per assembly or not? | `boolean` | | | | + +## Annotation options + +| Parameter | Description | Type | Default | Required | Hidden | +| --------------------------- | --------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ | +| `braker_extra_args` | Extra arguments for BRAKER | `string` | | | | +| `braker_allow_isoforms` | Allow multiple isoforms for gene models | `boolean` | True | | | +| `liftoff_coverage` | Liftoff coverage parameter | `number` | 0.9 | | | +| `liftoff_identity` | Liftoff identity parameter | `number` | 0.9 | | | +| `eggnogmapper_evalue` | Only report alignments below or equal the e-value threshold | `number` | 1e-05 | | | +| `eggnogmapper_pident` | Only report alignments above or equal to the given percentage of identity (0-100) | `integer` | 35 | | | +| `eggnogmapper_purge_nohits` | Purge transcripts which do not have a hit against eggnog | `boolean` | | | | + +## Max job request options + +Set the top limit for requested resources for any single job. + +| Parameter | Description | Type | Default | Required | Hidden | +| ------------ | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | --------- | ------- | -------- | ------ | +| `max_cpus` | Maximum number of CPUs that can be requested for any single job.
HelpUse to set an upper-limit for the CPU requirement for each process. Should be an integer e.g. `--max_cpus 1`
| `integer` | 12 | | True | +| `max_memory` | Maximum amount of memory that can be requested for any single job.
HelpUse to set an upper-limit for the memory requirement for each process. Should be a string in the format integer-unit e.g. `--max_memory '8.GB'`
| `string` | 200.GB | | True | +| `max_time` | Maximum amount of time that can be requested for any single job.
HelpUse to set an upper-limit for the time requirement for each process. Should be a string in the format integer-unit e.g. `--max_time '2.h'`
| `string` | 7.day | | True | diff --git a/local_pangene b/local_pangene index d242a68..9befef7 100755 --- a/local_pangene +++ b/local_pangene @@ -12,11 +12,11 @@ F_BOLD="\033[1m" && echo 'Executing with -stub' \ || echo -e "${C_RED}${F_BOLD}Executing without -stub${NO_FORMAT}" -nextflow \ +nextflow run \ main.nf \ -profile local,docker \ -resume \ $stub \ - -params-file conf/test_params.json \ + -params-file pangene-test/test_params.json \ --eggnogmapper_db_dir ../../dbs/emapperdb/5.0.2 \ --eggnogmapper_tax_scope 33090 diff --git a/modules/kherronism/braker3/main.nf b/modules/kherronism/braker3/main.nf index ae0ec81..82de764 100644 --- a/modules/kherronism/braker3/main.nf +++ b/modules/kherronism/braker3/main.nf @@ -62,9 +62,11 @@ process BRAKER3 { """ stub: + def args = task.ext.args ?: '' prefix = task.ext.prefix ?: "${meta.id}" def rna_ids = rnaseq_sets_ids ? "--rnaseq_sets_ids=${rnaseq_sets_ids}" : '' def touch_hints = (rna_ids || bam || proteins || hints) ? "touch ${prefix}/hintsfile.gff" : '' + def touch_gff = args.contains('--gff3') ? "touch ${prefix}/braker.gff3" : '' """ mkdir "$prefix" @@ -74,6 +76,7 @@ process BRAKER3 { $touch_hints touch "${prefix}/braker.log" touch "${prefix}/what-to-cite.txt" + $touch_gff cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/nextflow.config b/nextflow.config index 5e4e182..f8e24a0 100644 --- a/nextflow.config +++ b/nextflow.config @@ -3,8 +3,10 @@ includeConfig './conf/base.config' params { // Input/output options input = null - fastq = null external_protein_fastas = null + eggnogmapper_db_dir = null + eggnogmapper_tax_scope = null + fastq = null liftoff_annotations = null outdir = "./results" @@ -35,8 +37,6 @@ params { braker_allow_isoforms = true liftoff_coverage = 0.9 liftoff_identity = 0.9 - eggnogmapper_db_dir = null - eggnogmapper_tax_scope = null eggnogmapper_evalue = 0.00001 eggnogmapper_pident = 35 eggnogmapper_purge_nohits = false @@ -44,7 +44,7 @@ params { // Max job request options max_cpus = 12 max_memory = '200.GB' - max_time = '1.day' + max_time = '7.day' // Infrastructure options validationSkipDuplicateCheck= true @@ -58,7 +58,7 @@ manifest { description = """A NextFlow pipeline for pan-genome annotation""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.4' - version = '0.2.0+dev' + version = '0.2.0' doi = '' } diff --git a/nextflow_schema.json b/nextflow_schema.json index f1081eb..988f87a 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "", - "required": ["input", "outdir"], + "required": ["input", "outdir", "external_protein_fastas", "eggnogmapper_db_dir", "eggnogmapper_tax_scope"], "properties": { "input": { "type": "string", @@ -22,6 +22,24 @@ "fa_icon": "fas fa-file-csv", "help_text": "FASTA and other associated files for target assemblies provided as a CSV sheet" }, + "external_protein_fastas": { + "type": "string", + "description": "External protein fastas listed in a text sheet", + "help_text": "A text file listing FASTA files to provide protein evidence for annotation", + "format": "file-path", + "mimetype": "text/txt", + "fa_icon": "far fa-file-alt" + }, + "eggnogmapper_db_dir": { + "type": "string", + "description": "Eggnogmapper database directory", + "format": "directory-path" + }, + "eggnogmapper_tax_scope": { + "type": "integer", + "description": "Eggnogmapper taxonomy scopre", + "minimum": 0 + }, "fastq": { "type": "string", "format": "file-path", @@ -32,14 +50,6 @@ "fa_icon": "fas fa-file-csv", "description": "FASTQ samples listed in a CSV sheet" }, - "external_protein_fastas": { - "type": "string", - "description": "External protein fastas listed in a text sheet", - "help_text": "A text file listing FASTA files to provide protein evidence for annotation", - "format": "file-path", - "mimetype": "text/txt", - "fa_icon": "far fa-file-alt" - }, "liftoff_annotations": { "type": "string", "format": "file-path", @@ -184,16 +194,6 @@ "default": 0.9, "description": "Liftoff identity parameter" }, - "eggnogmapper_db_dir": { - "type": "string", - "description": "Eggnogmapper database directory", - "format": "directory-path" - }, - "eggnogmapper_tax_scope": { - "type": "integer", - "description": "Eggnogmapper taxonomy scopre", - "minimum": 0 - }, "eggnogmapper_evalue": { "type": "number", "default": 1e-5, @@ -208,7 +208,6 @@ }, "eggnogmapper_purge_nohits": { "type": "boolean", - "default": false, "description": "Purge transcripts which do not have a hit against eggnog" } } @@ -240,7 +239,7 @@ "max_time": { "type": "string", "description": "Maximum amount of time that can be requested for any single job.", - "default": "1.day", + "default": "7.day", "fa_icon": "far fa-clock", "pattern": "^(\\d+\\.?\\s*(s|m|h|d|day)\\s*)+$", "hidden": true, diff --git a/pfr/profile.config b/pfr/profile.config new file mode 100644 index 0000000..cec9a5c --- /dev/null +++ b/pfr/profile.config @@ -0,0 +1,17 @@ +profiles { + pfr { + process { + executor = 'slurm' + } + + apptainer { + envWhitelist = "APPTAINER_BINDPATH,APPTAINER_BIND" + cacheDir = "/workspace/pangene/singularity" + } + } +} + +params { + config_profile_name = 'Plant&Food profile' + config_profile_description = 'Plant&Food profile using SLURM in combination with Apptainer' +} diff --git a/pfr_pangene b/pfr_pangene index cda85f0..4f9bf4c 100644 --- a/pfr_pangene +++ b/pfr_pangene @@ -2,7 +2,7 @@ #SBATCH --job-name PANGENE -#SBATCH --time=1-00:00:00 +#SBATCH --time=7-00:00:00 #SBATCH --nodes=1 #SBATCH --ntasks=1 #SBATCH --cpus-per-task=1 @@ -10,13 +10,40 @@ #SBATCH --error pfr_pangene.stderr #SBATCH --mem=4G +full_test_flag=0 + +# Parse command line options +while getopts "t" opt; do + case ${opt} in + t ) + full_test_flag=1 + ;; + \? ) + echo "Invalid option: $OPTARG" 1>&2 + exit 1 + ;; + esac +done +shift $((OPTIND -1)) + +ml unload perl ml apptainer/1.1 ml nextflow/23.04.4 export TMPDIR="/workspace/$USER/tmp" export APPTAINER_BINDPATH="$APPTAINER_BINDPATH,$TMPDIR:$TMPDIR,$TMPDIR:/tmp" -nextflow \ - main.nf \ - -profile pfr,apptainer \ - -resume +if [ $full_test_flag -eq 1 ]; then + nextflow \ + main.nf \ + -c pfr/profile.config \ + -profile pfr,apptainer,test_full \ + -resume +else + nextflow \ + main.nf \ + -c pfr/profile.config \ + -profile pfr,apptainer \ + -params-file pfr/params.json \ + -resume +fi diff --git a/subworkflows/local/gff_eggnogmapper.nf b/subworkflows/local/gff_eggnogmapper.nf index 38d9673..7ea0d19 100644 --- a/subworkflows/local/gff_eggnogmapper.nf +++ b/subworkflows/local/gff_eggnogmapper.nf @@ -25,7 +25,7 @@ workflow GFF_EGGNOGMAPPER { ch_eggnogmapper_inputs = ch_gffread_fasta - | combine(db_folder ? Channel.fromPath(db_folder) : []) + | combine(Channel.fromPath(db_folder)) EGGNOGMAPPER( ch_eggnogmapper_inputs.map { meta, fasta, db -> [ meta, fasta ] }, diff --git a/subworkflows/local/gff_merge_cleanup.nf b/subworkflows/local/gff_merge_cleanup.nf index 286ea45..834af94 100644 --- a/subworkflows/local/gff_merge_cleanup.nf +++ b/subworkflows/local/gff_merge_cleanup.nf @@ -25,8 +25,8 @@ workflow GFF_MERGE_CLEANUP { ) ch_merged_gff = AGAT_SPMERGEANNOTATIONS.out.gff - | mix(ch_gff_branch.liftoff_only) - | mix(ch_gff_branch.braker_only) + | mix ( ch_gff_branch.liftoff_only.map { meta, braker_gff, liftoff_gff -> [ meta, liftoff_gff ] } ) + | mix ( ch_gff_branch.braker_only.map { meta, braker_gff, liftoff_gff -> [ meta, braker_gff ] } ) ch_versions = ch_versions.mix(AGAT_SPMERGEANNOTATIONS.out.versions.first()) // MODULE: GT_GFF3 diff --git a/subworkflows/local/gff_store.nf b/subworkflows/local/gff_store.nf index f487a50..7485300 100644 --- a/subworkflows/local/gff_store.nf +++ b/subworkflows/local/gff_store.nf @@ -32,7 +32,7 @@ workflow GFF_STORE { def gene_tx_annotations = [:] gff.readLines() .findAll { line -> - if ( line.startsWith('#') ) { return false } + if ( line.startsWith('#') || line == '' ) { return false } def cols = line.split('\t') def feat = cols[2] @@ -76,7 +76,7 @@ workflow GFF_STORE { def gff_lines = gff.readLines() .collect { line -> - if ( line.startsWith('#') ) { return line } + if ( line.startsWith('#') || line == '' ) { return line } def cols = line.split('\t') def feat = cols[2] diff --git a/subworkflows/local/purge_nohit_models.nf b/subworkflows/local/purge_nohit_models.nf index 5c22f5f..d213dc2 100644 --- a/subworkflows/local/purge_nohit_models.nf +++ b/subworkflows/local/purge_nohit_models.nf @@ -20,9 +20,10 @@ workflow PURGE_NOHIT_MODELS { .unique() def tx_in_gff = gff.readLines() - .findAll { it -> - if ( it.startsWith('#') ) { return false } - def feat = it.split('\t')[2] + .findAll { line -> + if ( line.startsWith('#') || line == '' ) { return false } + + def feat = line.split('\t')[2] ( feat == 'transcript' || feat == 'mRNA' ) } .collect { it -> diff --git a/TODO.md b/tests/stub/emapperdb/5.0.2/eggnog.db similarity index 100% rename from TODO.md rename to tests/stub/emapperdb/5.0.2/eggnog.db diff --git a/tests/stub/emapperdb/5.0.2/eggnog_proteins.dmnd b/tests/stub/emapperdb/5.0.2/eggnog_proteins.dmnd new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/external-protein-fastas.txt b/tests/stub/external-protein-fastas.txt index 33b3484..d510c4c 100644 --- a/tests/stub/external-protein-fastas.txt +++ b/tests/stub/external-protein-fastas.txt @@ -1,2 +1,2 @@ -pangene-test/ext_prot/RU01.20221115150135.chr1.pep.fasta.gz -pangene-test/ext_prot/RU01.20221115150135.chr2.pep.fasta.gz +tests/stub/ext_prot/RU01.20221115150135.chr1.pep.fasta.gz +tests/stub/ext_prot/RU01.20221115150135.chr2.pep.fasta.gz diff --git a/tests/stub/liftoffannotations.csv b/tests/stub/liftoffannotations.csv index 8e1da9e..5215d2c 100644 --- a/tests/stub/liftoffannotations.csv +++ b/tests/stub/liftoffannotations.csv @@ -1,3 +1,3 @@ fasta,gff3 -pangene-test/liftoff/Russell_V2a.chr1.fsa.gz,pangene-test/liftoff/RU01.20221115150135.chr1.gff3.gz -pangene-test/liftoff/Russell_V2a.chr2.fsa.gz,pangene-test/liftoff/RU01.20221115150135.chr2.gff3.gz +tests/stub/liftoff/Russell_V2a.chr1.fsa.gz,tests/stub/liftoff/RU01.20221115150135.chr1.gff3.gz +tests/stub/liftoff/Russell_V2a.chr2.fsa.gz,tests/stub/liftoff/RU01.20221115150135.chr2.gff3.gz diff --git a/tests/stub/test_stub.json b/tests/stub/test_stub.json new file mode 100644 index 0000000..c49c3e9 --- /dev/null +++ b/tests/stub/test_stub.json @@ -0,0 +1,9 @@ +{ + "input": "tests/stub/assemblysheet.csv", + "eggnogmapper_db_dir": "tests/stub/emapperdb/5.0.2", + "external_protein_fastas": "tests/stub/external-protein-fastas.txt", + "fastq": "tests/stub/fastqsheet.csv", + "liftoff_annotations": "tests/stub/liftoffannotations.csv", + "max_cpus": 2, + "max_memory": "3.GB" +} diff --git a/workflows/pangene.nf b/workflows/pangene.nf index 66a0459..b753b67 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -127,8 +127,13 @@ workflow PANGENE { gff: [ [ id: idFromFileName( fastaFile.baseName ) ], file(gff, checkIfExists:true) ] } - ch_liftoff_fasta = ch_liftoff_mm.fasta - ch_liftoff_gff = ch_liftoff_mm.gff + ch_liftoff_fasta = params.liftoff_annotations + ? ch_liftoff_mm.fasta + : Channel.empty() + + ch_liftoff_gff = params.liftoff_annotations + ? ch_liftoff_mm.gff + : Channel.empty() val_tsebra_config = params.braker_allow_isoforms ? "${projectDir}/assets/tsebra-default.cfg" From cc6f3915b80167b6459d70326310dd17874cdb24 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Wed, 24 Apr 2024 18:03:36 +1200 Subject: [PATCH 31/40] Updated flowchart --- .editorconfig | 4 + README.md | 78 +------- docs/img/pangene.drawio | 432 ++++++++++++++++++++++++++++++++++++++++ docs/img/pangene.png | Bin 0 -> 808977 bytes 4 files changed, 437 insertions(+), 77 deletions(-) create mode 100644 docs/img/pangene.drawio create mode 100644 docs/img/pangene.png diff --git a/.editorconfig b/.editorconfig index 82dc73a..2813def 100644 --- a/.editorconfig +++ b/.editorconfig @@ -60,6 +60,10 @@ indent_style = unset [*.{pl,pm}] indent_size = unset +# ignore drawio +[*.drawio] +indent_size = unset + # ignore LICENSE [LICENSE] indent_size = unset diff --git a/README.md b/README.md index 65d08cf..e8bb274 100644 --- a/README.md +++ b/README.md @@ -6,83 +6,7 @@ A NextFlow pipeline for pan-genome annotation. It can also be used for annotatio ## Pipeline Flowchart -```mermaid -flowchart TD - subgraph PrepareAssembly [ ] - TARGET_ASSEMBLIES - TE_LIBRARIES - FASTA_VALIDATE - fasta_file_from_fasta_validate - EDTA - REPEATMODELER - te_lib_absent_node - REPEATMASKER - end - - TARGET_ASSEMBLIES(["[target_assemblies]"]) - TE_LIBRARIES(["[te_libs]"]) - TARGET_ASSEMBLIES --> FASTA_VALIDATE - FASTA_VALIDATE --- |Fasta|fasta_file_from_fasta_validate(( )) - fasta_file_from_fasta_validate --> |or|EDTA - fasta_file_from_fasta_validate --> |default|REPEATMODELER - REPEATMODELER --- te_lib_absent_node(( )) - EDTA --- te_lib_absent_node - TE_LIBRARIES --> REPEATMASKER - te_lib_absent_node --> REPEATMASKER - - subgraph Fastq [ ] - FASTQ - CAT_FASTQ - FASTQC - FASTP - FASTP_FASTQC - SORTMERNA - fasta_file_for_star - STAR - SAMTOOLS_CAT - end - - FASTQ([fastq]) - FASTQ --> |Tech. reps|CAT_FASTQ - CAT_FASTQ --> FASTQC - FASTQ --> FASTQC - FASTQC --> FASTP - FASTP --> FASTP_FASTQC[FASTQC] - FASTP_FASTQC --> SORTMERNA - fasta_file_for_star(( )) - fasta_file_for_star --> |Fasta|STAR - SORTMERNA --> STAR - STAR --> SAMTOOLS_CAT - - subgraph Annotation [ ] - anno_fasta(( )) - anno_masked_fasta(( )) - anno_bam(( )) - EXTERNAL_PROTEIN_SEQS(["[ext_prots]"]) - XREF_ANNOTATIONS(["[xref_annotations]"]) - CAT - BRAKER3 - GFFREAD - LIFTOFF - end - - PrepareAssembly --> |Fasta, Masked fasta|Annotation - Fastq --> |RNASeq bam|Annotation - - XREF_ANNOTATIONS --> |xref_gff|GFFREAD - XREF_ANNOTATIONS --> |xref_fasta|LIFTOFF - GFFREAD --> LIFTOFF - anno_fasta --> |Fasta|LIFTOFF - - EXTERNAL_PROTEIN_SEQS --> CAT - anno_masked_fasta --> |Masked fasta|BRAKER3 - anno_bam --> |RNASeq bam|BRAKER3 - CAT --> BRAKER3 - - style Fastq fill:#00FFFF21,stroke:#00FFFF21 - style PrepareAssembly fill:#00FFFF21,stroke:#00FFFF21 - style Annotation fill:#00FFFF21,stroke:#00FFFF21 -``` +
## Alpha Release diff --git a/docs/img/pangene.drawio b/docs/img/pangene.drawio new file mode 100644 index 0000000..4f717f2 --- /dev/null +++ b/docs/img/pangene.drawio @@ -0,0 +1,432 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + 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100644 --- a/README.md +++ b/README.md @@ -6,7 +6,7 @@ A NextFlow pipeline for pan-genome annotation. It can also be used for annotatio ## Pipeline Flowchart -

    +

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Apr 2024 19:11:12 +1200 Subject: [PATCH 34/40] Updated flowchart --- docs/img/pangene.drawio | 120 ++++++++++++++++++++-------------------- docs/img/pangene.png | Bin 844984 -> 863296 bytes 2 files changed, 60 insertions(+), 60 deletions(-) diff --git a/docs/img/pangene.drawio b/docs/img/pangene.drawio index d274b04..0c61685 100644 --- a/docs/img/pangene.drawio +++ b/docs/img/pangene.drawio @@ -1,6 +1,6 @@ - + - + @@ -28,49 +28,49 @@ - + - - + + - + - + - + - + - + - - + + - + - + - + - + - + @@ -94,16 +94,16 @@ - + - + - + @@ -122,7 +122,7 @@ - + @@ -131,7 +131,7 @@ - + @@ -141,34 +141,34 @@ - + - + - + - + - + - + - + @@ -204,10 +204,10 @@ - + - + @@ -222,10 +222,10 @@ - - + + - + @@ -274,10 +274,10 @@ - - + + - + @@ -322,17 +322,17 @@ - + - + - - + + @@ -340,37 +340,37 @@ - - + + - + - + - + - + - - + + - + - - + + - + @@ -389,8 +389,8 @@ - - + + @@ -401,10 +401,10 @@ - - + + - + @@ -423,8 +423,8 @@ - - + + diff --git a/docs/img/pangene.png b/docs/img/pangene.png index 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2024 10:53:55 +1200 Subject: [PATCH 35/40] Updated README --- README.md | 8 ++++---- 1 file changed, 4 insertions(+), 4 deletions(-) diff --git a/README.md b/README.md index 8d95136..d255a36 100644 --- a/README.md +++ b/README.md @@ -1,10 +1,10 @@ # PANGENE -[![Lint and -stub on Linux/Docker](https://github.com/PlantandFoodResearch/pangene/actions/workflows/test.yml/badge.svg)](https://github.com/PlantandFoodResearch/pangene/actions/workflows/test.yml) +[![Lint/stub on Linux/Docker](https://github.com/PlantandFoodResearch/pangene/actions/workflows/test.yml/badge.svg)](https://github.com/PlantandFoodResearch/pangene/actions/workflows/test.yml) A NextFlow pipeline for pan-genome annotation. It can also be used for annotation of a single genome. -## Pipeline Flowchart +## Flowchart

      @@ -14,7 +14,7 @@ This release is not fully documented and under alpha testing by the Bioinformati ## Plant&Food Users -Download the pipeline to your `/workspace/$USER` folder. Change the parameters defined in the [pfr/params.json](./pfr/params.json) file. Submit the pipeline to SLURM for execution. For a description of the parameters, see [parameters](./docs/parameters.md). +Download the pipeline to your `/workspace/$USER` folder. Change the parameters defined in the [pfr/params.json](./pfr/params.json) file. Submit the pipeline to SLURM for execution. For a description of the parameters, see [parameters.md](./docs/parameters.md). ```bash sbatch ./pfr_pangene @@ -22,7 +22,7 @@ sbatch ./pfr_pangene ## Credits -plantandfoodresearch/pangene scripts were originally written by Jason Shiller. Usman Rashid wrote the NextFLow pipeline. +plantandfoodresearch/pangene workflows were originally scripted by Jason Shiller. Usman Rashid wrote the NextFLow pipeline. We thank the following people for their extensive assistance in the development of this pipeline. From bffdb63da52352c7934eda0173cfaac16311ec33 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 29 Apr 2024 11:40:14 +1200 Subject: [PATCH 36/40] Now removing iso-forms left by TSEBRA using AGAT_SPFILTERFEATUREFROMKILLLIST --- CHANGELOG.md | 1 + conf/modules.config | 4 ++ subworkflows/local/purge_breaker_models.nf | 77 +++++++++++++++++++--- workflows/pangene.nf | 3 +- 4 files changed, 74 insertions(+), 11 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 7234e70..135fb02 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -46,6 +46,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 37. Add a document for the pipeline parameters 38. Updated `pfr_pangene` and `pfr/profile.config` 39. Now using local tests/stub files for GitHub CI +40. Now removing iso-forms left by TSEBRA using `AGAT_SPFILTERFEATUREFROMKILLLIST` ### `Fixed` diff --git a/conf/modules.config b/conf/modules.config index 66163de..f35a165 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -191,6 +191,10 @@ process { ext.args = '--gtf_version relax' } + withName: 'KILL_TSEBRA_ISOFORMS' { + ext.prefix = { "${meta.id}.1form" } + } + withName: 'AGAT_SPFILTERFEATUREFROMKILLLIST' { ext.prefix = { "${meta.id}.purged" } } diff --git a/subworkflows/local/purge_breaker_models.nf b/subworkflows/local/purge_breaker_models.nf index 6633246..db828ab 100644 --- a/subworkflows/local/purge_breaker_models.nf +++ b/subworkflows/local/purge_breaker_models.nf @@ -1,10 +1,11 @@ -include { AGAT_CONVERTSPGFF2GTF } from '../../modules/nf-core/agat/convertspgff2gtf/main' -include { TSEBRA } from '../../modules/pfr/tsebra/main' -include { AGAT_CONVERTSPGXF2GXF } from '../../modules/nf-core/agat/convertspgxf2gxf/main' -include { GFFCOMPARE as COMPARE_BRAKER_TO_LIFTOFF } from '../../modules/nf-core/gffcompare/main' -include { AGAT_SPFILTERFEATUREFROMKILLLIST } from '../../modules/pfr/agat/spfilterfeaturefromkilllist/main' -include { GFFCOMPARE as VALIDATE_PURGING_BY_AGAT } from '../../modules/nf-core/gffcompare/main' -include { AGAT_SPMERGEANNOTATIONS as MERGE_BRAKER_LIFTOFF } from '../../modules/pfr/agat/spmergeannotations/main' +include { AGAT_CONVERTSPGFF2GTF } from '../../modules/nf-core/agat/convertspgff2gtf/main' +include { TSEBRA } from '../../modules/pfr/tsebra/main' +include { AGAT_CONVERTSPGXF2GXF } from '../../modules/nf-core/agat/convertspgxf2gxf/main' +include { AGAT_SPFILTERFEATUREFROMKILLLIST as KILL_TSEBRA_ISOFORMS } from '../../modules/pfr/agat/spfilterfeaturefromkilllist/main' +include { GFFCOMPARE as COMPARE_BRAKER_TO_LIFTOFF } from '../../modules/nf-core/gffcompare/main' +include { AGAT_SPFILTERFEATUREFROMKILLLIST } from '../../modules/pfr/agat/spfilterfeaturefromkilllist/main' +include { GFFCOMPARE as VALIDATE_PURGING_BY_AGAT } from '../../modules/nf-core/gffcompare/main' +include { AGAT_SPMERGEANNOTATIONS as MERGE_BRAKER_LIFTOFF } from '../../modules/pfr/agat/spmergeannotations/main' workflow PURGE_BREAKER_MODELS { take: @@ -12,6 +13,7 @@ workflow PURGE_BREAKER_MODELS { braker_hints // [ meta, gff ] liftoff_gff3 // [ meta, gff3 ] tsebra_config // val(tsebra_config) + braker_allow_isoforms // val(true|false) main: ch_versions = Channel.empty() @@ -115,8 +117,63 @@ workflow PURGE_BREAKER_MODELS { [ [ id: file.baseName ], file ] } + // COLLECTFILE: Iso-form kill list if braker_allow_isoforms=true + ch_post_tsebra_kill_list = braker_allow_isoforms + ? Channel.empty() + : ch_tsebra_gff + | map { meta, gff -> + def kill_list = gff.readLines() + .findAll { line -> + if ( line.startsWith('#') ) { return false } + + def cols = line.split('\t') + def feat = cols[2] + + ( feat == 'mRNA' || feat == 'transcript' ) + } + .collect { line -> + def cols = line.split('\t') + def atts = cols[8] + def tx_id = ( atts =~ /ID=([^;]*)/ )[0][1] + def g_id = ( atts =~ /Parent=([^;]*)/ )[0][1] + + [ g_id, tx_id ] + } + .groupBy { g_id, tx_id -> g_id } + .findAll { key, value -> value.size() > 1 } + .collect { key, value -> + value.collect { it[1] }[1..-1] + } + .flatten() + .join('\n') + + [ "${meta.id}.kill.list.txt" ] + [ kill_list ] + } + | collectFile(newLine: true) + | map { file -> + [ [ id: file.baseName.replace('.kill.list', '') ], file ] + } + + // MODULE: AGAT_SPFILTERFEATUREFROMKILLLIST as KILL_TSEBRA_ISOFORMS + ch_tsebra_kill_inputs = ch_tsebra_gff + | join(ch_post_tsebra_kill_list) + + + KILL_TSEBRA_ISOFORMS( + ch_tsebra_kill_inputs.map { meta, gff, kill -> [ meta, gff ] }, + ch_tsebra_kill_inputs.map { meta, gff, kill -> kill }, + [] // default config + ) + + ch_tsebra_killed_gff = ch_tsebra_gff + | join(KILL_TSEBRA_ISOFORMS.out.gff, remainder: true) + | map { meta, tsebra, killed -> + if ( tsebra ) { [ meta, killed ?: tsebra ] } + } + ch_versions = ch_versions.mix(KILL_TSEBRA_ISOFORMS.out.versions.first()) + // MODULE: GFFCOMPARE as COMPARE_BRAKER_TO_LIFTOFF - ch_comparison_inputs = ch_tsebra_gff + ch_comparison_inputs = ch_tsebra_killed_gff | join(liftoff_gff3) @@ -157,7 +214,7 @@ workflow PURGE_BREAKER_MODELS { } // MODULE: AGAT_SPFILTERFEATUREFROMKILLLIST - ch_agat_kill_inputs = ch_tsebra_gff + ch_agat_kill_inputs = ch_tsebra_killed_gff | join(ch_kill_list) @@ -171,7 +228,7 @@ workflow PURGE_BREAKER_MODELS { ch_versions = ch_versions.mix(AGAT_SPFILTERFEATUREFROMKILLLIST.out.versions.first()) // Handle case where liftoff is not present - ch_all_braker_gff = ch_tsebra_gff + ch_all_braker_gff = ch_tsebra_killed_gff | join(ch_braker_purged_gff, remainder:true) | map { meta, tsebra_gff, purged_gff -> if ( purged_gff ) { return [ meta, purged_gff ] } diff --git a/workflows/pangene.nf b/workflows/pangene.nf index b753b67..c0f3c32 100644 --- a/workflows/pangene.nf +++ b/workflows/pangene.nf @@ -215,7 +215,8 @@ workflow PANGENE { ch_braker_gff3, ch_braker_hints, ch_liftoff_gff3, - val_tsebra_config + val_tsebra_config, + params.braker_allow_isoforms ) ch_braker_purged_gff = PURGE_BREAKER_MODELS.out.braker_purged_gff From 099b76d782cbb396e3cbfa9fb6f9b07a035b4caf Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 29 Apr 2024 11:55:40 +1200 Subject: [PATCH 37/40] Added pyproject.toml --- .github/workflows/test.yml | 2 +- CHANGELOG.md | 1 + local_pangene | 5 ++--- pyproject.toml | 15 +++++++++++++++ tests/stub/{test_stub.json => params.json} | 3 ++- 5 files changed, 21 insertions(+), 5 deletions(-) create mode 100644 pyproject.toml rename tests/stub/{test_stub.json => params.json} (89%) diff --git a/.github/workflows/test.yml b/.github/workflows/test.yml index da21d2f..04497a0 100644 --- a/.github/workflows/test.yml +++ b/.github/workflows/test.yml @@ -50,7 +50,7 @@ jobs: main.nf \ -profile local,docker \ -stub \ - -params-file tests/stub/test_stub.json + -params-file tests/stub/params.json confirm-pass: runs-on: ubuntu-latest diff --git a/CHANGELOG.md b/CHANGELOG.md index 135fb02..7936734 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -47,6 +47,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 38. Updated `pfr_pangene` and `pfr/profile.config` 39. Now using local tests/stub files for GitHub CI 40. Now removing iso-forms left by TSEBRA using `AGAT_SPFILTERFEATUREFROMKILLLIST` +41. Added `pyproject.toml` ### `Fixed` diff --git a/local_pangene b/local_pangene index 9befef7..8a1aa8a 100755 --- a/local_pangene +++ b/local_pangene @@ -17,6 +17,5 @@ nextflow run \ -profile local,docker \ -resume \ $stub \ - -params-file pangene-test/test_params.json \ - --eggnogmapper_db_dir ../../dbs/emapperdb/5.0.2 \ - --eggnogmapper_tax_scope 33090 + -params-file pangene-test/params.json \ + --eggnogmapper_db_dir ../dbs/emapperdb/5.0.2 diff --git a/pyproject.toml b/pyproject.toml new file mode 100644 index 0000000..5611062 --- /dev/null +++ b/pyproject.toml @@ -0,0 +1,15 @@ +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. +# Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. +[tool.ruff] +line-length = 120 +target-version = "py38" +cache-dir = "~/.cache/ruff" + +[tool.ruff.lint] +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] + +[tool.ruff.lint.isort] +known-first-party = ["nf_core"] + +[tool.ruff.lint.per-file-ignores] +"__init__.py" = ["E402", "F401"] diff --git a/tests/stub/test_stub.json b/tests/stub/params.json similarity index 89% rename from tests/stub/test_stub.json rename to tests/stub/params.json index c49c3e9..db97d3b 100644 --- a/tests/stub/test_stub.json +++ b/tests/stub/params.json @@ -1,7 +1,8 @@ { "input": "tests/stub/assemblysheet.csv", - "eggnogmapper_db_dir": "tests/stub/emapperdb/5.0.2", "external_protein_fastas": "tests/stub/external-protein-fastas.txt", + "eggnogmapper_db_dir": "tests/stub/emapperdb/5.0.2", + "eggnogmapper_tax_scope": 33090, "fastq": "tests/stub/fastqsheet.csv", "liftoff_annotations": "tests/stub/liftoffannotations.csv", "max_cpus": 2, From 7e338d6f695f0d0456440d66bde1b5d2e37bbdac Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 29 Apr 2024 12:06:39 +1200 Subject: [PATCH 38/40] Version bumped to 0.3.0 --- CHANGELOG.md | 2 +- nextflow.config | 2 +- tests/stub/assemblysheet.csv | 6 +++--- tests/stub/braker/donghong.gff3.gz | 0 tests/stub/braker/donghong.hints.gff.gz | 0 tests/stub/braker/red5_v2p1.gff3.gz | 0 tests/stub/braker/red5_v2p1.hints.gff.gz | 0 7 files changed, 5 insertions(+), 5 deletions(-) create mode 100644 tests/stub/braker/donghong.gff3.gz create mode 100644 tests/stub/braker/donghong.hints.gff.gz create mode 100644 tests/stub/braker/red5_v2p1.gff3.gz create mode 100644 tests/stub/braker/red5_v2p1.hints.gff.gz diff --git a/CHANGELOG.md b/CHANGELOG.md index 7936734..9477ab8 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 0.2.0 - [24-April-2024] +## 0.3.0 - [29-April-2024] ### `Added` diff --git a/nextflow.config b/nextflow.config index f8e24a0..a9ae34a 100644 --- a/nextflow.config +++ b/nextflow.config @@ -58,7 +58,7 @@ manifest { description = """A NextFlow pipeline for pan-genome annotation""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.4' - version = '0.2.0' + version = '0.3.0' doi = '' } diff --git a/tests/stub/assemblysheet.csv b/tests/stub/assemblysheet.csv index d1d9be7..cfa0cdb 100644 --- a/tests/stub/assemblysheet.csv +++ b/tests/stub/assemblysheet.csv @@ -1,3 +1,3 @@ -tag,fasta,is_masked,te_lib -red5_v2p1,tests/stub/target/red5_v2p1_chr1.fasta.gz,no, -donghong,tests/stub/target/donghong.chr1.fsa.gz,no,tests/stub/te_lib/donghong.TElib.fa.gz +tag,fasta,is_masked,te_lib,braker_gff3,braker_hints +red5_v2p1,tests/stub/target/red5_v2p1_chr1.fasta.gz,no,,tests/stub/braker/red5_v2p1.gff3.gz,tests/stub/braker/red5_v2p1.hints.gff.gz +donghong,tests/stub/target/donghong.chr1.fsa.gz,no,tests/stub/te_lib/donghong.TElib.fa.gz,tests/stub/braker/red5_v2p1.gff3.gz,tests/stub/braker/red5_v2p1.hints.gff.gz diff --git a/tests/stub/braker/donghong.gff3.gz b/tests/stub/braker/donghong.gff3.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/braker/donghong.hints.gff.gz b/tests/stub/braker/donghong.hints.gff.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/braker/red5_v2p1.gff3.gz b/tests/stub/braker/red5_v2p1.gff3.gz new file mode 100644 index 0000000..e69de29 diff --git a/tests/stub/braker/red5_v2p1.hints.gff.gz b/tests/stub/braker/red5_v2p1.hints.gff.gz new file mode 100644 index 0000000..e69de29 From 53088d7c142767025e9c5740e1c3ddeeb001389a Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Mon, 29 Apr 2024 15:49:51 +1200 Subject: [PATCH 39/40] Updated PFR params --- conf/modules.config | 2 +- modules/nf-core/fastqc/main.nf | 1 - pfr/params.json | 20 ++++++-------------- pfr/profile.config | 8 ++++---- 4 files changed, 11 insertions(+), 20 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index f35a165..f5e0cbf 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -43,7 +43,7 @@ process { if(!params.skip_fastqc) { process { withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:FASTQC_RAW' { - ext.args = '--quiet' + ext.args = '--quiet' } withName: '.*:FASTQ_FASTQC_UMITOOLS_FASTP:FASTQC_TRIM' { diff --git a/modules/nf-core/fastqc/main.nf b/modules/nf-core/fastqc/main.nf index d79f1c8..1fd7ac4 100644 --- a/modules/nf-core/fastqc/main.nf +++ b/modules/nf-core/fastqc/main.nf @@ -38,7 +38,6 @@ process FASTQC { fastqc \\ $args \\ --threads $task.cpus \\ - --memory $fastqc_memory \\ $renamed_files cat <<-END_VERSIONS > versions.yml diff --git a/pfr/params.json b/pfr/params.json index e8ca7e4..cb7d801 100644 --- a/pfr/params.json +++ b/pfr/params.json @@ -1,16 +1,8 @@ { - "external_protein_fastas": [ - "/workspace/ComparativeDataSources/OrthoDB11/Viridiplantae.fa.gz", - "/output/genomic/fairGenomes/Plant/Actinidia/chinensis/var_chinensis/male/2x/assembly_russell/v2.1/RU01.20221115150135.pep.fasta" - ], - "liftoff": [ - [ - "/output/genomic/fairGenomes/Plant/Actinidia/chinensis/var_chinensis/male/2x/assembly_russell/v2.1/Russell_V2a.chromosomes.and.unassiged.and.haplotigs.fsa", - "/output/genomic/fairGenomes/Plant/Actinidia/chinensis/var_chinensis/male/2x/assembly_russell/v2.1/RU01.20221115150135.gff3" - ], - [ - "/output/genomic/fairGenomes/Plant/Arabidopsis/thaliana/var_na/sex_na/2x/assembly_tair/v10/TAIR10_chr_all.fas", - "/output/genomic/fairGenomes/Plant/Arabidopsis/thaliana/var_na/sex_na/2x/assembly_tair/v10/TAIR10_GFF3_genes_transposons.fixed.gff3" - ] - ] + "input": "/workspace/pangene/test_data/assemblysheet.csv", + "external_protein_fastas": "/workspace/pangene/test_data/external-protein-fastas.txt", + "eggnogmapper_db_dir": "/workspace/ComparativeDataSources/emapperdb/5.0.2", + "eggnogmapper_tax_scope": 33090, + "fastq": "/workspace/pangene/test_data/fastqsheet.csv", + "liftoff_annotations": "/workspace/pangene/test_data/liftoffannotations.csv" } diff --git a/pfr/profile.config b/pfr/profile.config index cec9a5c..b0eba29 100644 --- a/pfr/profile.config +++ b/pfr/profile.config @@ -11,7 +11,7 @@ profiles { } } -params { - config_profile_name = 'Plant&Food profile' - config_profile_description = 'Plant&Food profile using SLURM in combination with Apptainer' -} +// params { +// config_profile_name = 'Plant&Food profile' +// config_profile_description = 'Plant&Food profile using SLURM in combination with Apptainer' +// } From d11f8312238b31caebf953c1603d685e17b22694 Mon Sep 17 00:00:00 2001 From: Usman Rashid Date: Tue, 30 Apr 2024 12:49:13 +1200 Subject: [PATCH 40/40] Now using PFAMs from eggnog if description is '-' --- CHANGELOG.md | 3 ++- subworkflows/local/gff_store.nf | 17 +++++++++++------ 2 files changed, 13 insertions(+), 7 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 9477ab8..3eb74a0 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## 0.3.0 - [29-April-2024] +## 0.3.0 - [30-April-2024] ### `Added` @@ -48,6 +48,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 39. Now using local tests/stub files for GitHub CI 40. Now removing iso-forms left by TSEBRA using `AGAT_SPFILTERFEATUREFROMKILLLIST` 41. Added `pyproject.toml` +42. Now using PFAMs from eggnog if description is '-' ### `Fixed` diff --git a/subworkflows/local/gff_store.nf b/subworkflows/local/gff_store.nf index 7485300..3326e2a 100644 --- a/subworkflows/local/gff_store.nf +++ b/subworkflows/local/gff_store.nf @@ -20,12 +20,17 @@ workflow GFF_STORE { def cols = line.split('\t') def id = cols[0] def txt = cols[7] + def pfams = cols[20] - [ id, txt ] + [ id, txt, pfams ] + } + .collect { id, txt, pfams -> + if ( txt != '-' ) { return [ id, txt ] } + if ( pfams != '-' ) { return [ id, "PFAMs: $pfams" ] } + + [ id, 'No eggnog description and PFAMs' ] } - .findAll { id, txt -> - txt != '-' - }.collectEntries { id, txt -> + .collectEntries { id, txt -> [ id, txt ] } @@ -57,7 +62,7 @@ workflow GFF_STORE { def anno = tx_annotations.containsKey(tx_id) ? URLEncoder.encode(tx_annotations[tx_id], "UTF-8").replace('+', '%20') - : URLEncoder.encode('hypothetical protein | no eggnog hit', "UTF-8").replace('+', '%20') + : URLEncoder.encode('Hypothetical protein | no eggnog hit', "UTF-8").replace('+', '%20') gene_tx_annotations[gene_id] += [ ( tx_id ): anno ] } @@ -67,7 +72,7 @@ workflow GFF_STORE { def default_anno = tx_annos.values().first() if ( tx_annos.values().findAll { it != default_anno }.size() > 0 ) { - return [ gene_id, ( tx_annos + [ 'default': 'differing%20isoform%20descriptions' ] ) ] + return [ gene_id, ( tx_annos + [ 'default': 'Differing%20isoform%20descriptions' ] ) ] } [ gene_id, ( tx_annos + [ 'default': default_anno ] ) ]

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