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This repository has been archived by the owner on Mar 16, 2022. It is now read-only.
I installed the falcon version falcon-2017.06.28-18.01-py2.7-ucs4.tar.gz and run the fc_run.py script with the test greg200k-sv2 data and met an error like that :
[INFO]Apparently '/ds3512/home/panyp/denovo/falcon/ecoli2' is not in lustre filesystem, which is fine.
[INFO]fc_run started with configuration /ds3512/home/panyp/denovo/falcon/ecoli2/fc_run.cfg
[WARNING]You have several old-style options. These should be provided in the [job.defaults] or [job.step.*] sections, and possibly renamed. See https://g
ithub.com/PacificBiosciences/FALCON/wiki/Configuration
['job_type', 'sge_option_cns', 'sge_option_da', 'sge_option_fc', 'sge_option_la', 'sge_option_pda', 'sge_option_pla']
[WARNING]Unexpected keys in input config: set(['ovlp_HPCTANmask_option', 'pa_concurrent_jobs', 'sge_option_da', 'sge_option_pla', 'job_type', 'ovlp_concurren
t_jobs', 'jobqueue', 'sge_option_fc', 'sge_option_cns', 'sge_option_la', 'sge_option_pda'])
[WARNING]Please specify "job_type" only in the [job.defaults] section, not in [General].
[WARNING]Please supply a default for "njobs" (aka concurrency) in section [job.defaults]. For now, we will use 8
[INFO]cfg=
{
"General": {
"LA4Falcon_preload": false,
"avoid_text_file_busy": true,
"bestn": 12,
"dazcon": false,
"falcon_sense_greedy": false,
"falcon_sense_option": "--output-multi --min-idt 0.70 --min-cov 4 --max-n-read 200 --n-core 6",
"falcon_sense_skip_contained": false,
"fc_ovlp_to_graph_option": " --min_len 12000",
"genome_size": 0,
"input_fofn": "input.fofn",
....................................................................
Traceback (most recent call last):
File "/ds3512/home/panyp/denovo/falcon/falcon-py2.7-ucs4/bin/fc_run.py", line 5, in
main(sys.argv)
File "/ds3512/home/panyp/ruanjian/Python2.7/lib/python2.7/site-packages/falcon_kit/mains/run1.py", line 724, in main
main1(argv[0], args.config, args.logger)
File "/ds3512/home/panyp/ruanjian/Python2.7/lib/python2.7/site-packages/falcon_kit/mains/run1.py", line 60, in main1
check_general_config(general_config, input_config_fn)
File "/ds3512/home/panyp/ruanjian/Python2.7/lib/python2.7/site-packages/falcon_kit/mains/run1.py", line 40, in check_general_config
raise Exception(msg)
Exception: Missing options.
We now require both "pa_daligner_option" (stage 0) and "ovlp_daligner_option" (stage 1),
which are automatically passed along to
HPC.daligner
HPC.TANmask
HPC.REPmask
These can provide additional flags:
pa_HPCdaligner_option
pa_HPCTANmask_option
ovlp_HPCdaligner_option
pa_REPmask_code (-g/-c pairs for 3 iterations, e.g. '1,20;5,15;20,10')
I thought my configure file had someting wrong ,here is my fc_run.cfg.
[General]
job_type = SGE
list of files of the initial bas.h5 files
input_fofn = input.fofn
#input_fofn = preads.fofn
input_type = raw
#input_type = preads
The length cutoff used for seed reads used for initial mapping
length_cutoff = 12000
The length cutoff used for seed reads usef for pre-assembly
I installed the falcon version falcon-2017.06.28-18.01-py2.7-ucs4.tar.gz and run the fc_run.py script with the test greg200k-sv2 data and met an error like that :
[INFO]Apparently '/ds3512/home/panyp/denovo/falcon/ecoli2' is not in lustre filesystem, which is fine.
[INFO]fc_run started with configuration /ds3512/home/panyp/denovo/falcon/ecoli2/fc_run.cfg
[WARNING]You have several old-style options. These should be provided in the
[job.defaults]
or[job.step.*]
sections, and possibly renamed. See https://github.com/PacificBiosciences/FALCON/wiki/Configuration
['job_type', 'sge_option_cns', 'sge_option_da', 'sge_option_fc', 'sge_option_la', 'sge_option_pda', 'sge_option_pla']
[WARNING]Unexpected keys in input config: set(['ovlp_HPCTANmask_option', 'pa_concurrent_jobs', 'sge_option_da', 'sge_option_pla', 'job_type', 'ovlp_concurren
t_jobs', 'jobqueue', 'sge_option_fc', 'sge_option_cns', 'sge_option_la', 'sge_option_pda'])
[WARNING]Please specify "job_type" only in the [job.defaults] section, not in [General].
[WARNING]Please supply a default for "njobs" (aka concurrency) in section [job.defaults]. For now, we will use 8
[INFO]cfg=
{
"General": {
"LA4Falcon_preload": false,
"avoid_text_file_busy": true,
"bestn": 12,
"dazcon": false,
"falcon_sense_greedy": false,
"falcon_sense_option": "--output-multi --min-idt 0.70 --min-cov 4 --max-n-read 200 --n-core 6",
"falcon_sense_skip_contained": false,
"fc_ovlp_to_graph_option": " --min_len 12000",
"genome_size": 0,
"input_fofn": "input.fofn",
....................................................................
Traceback (most recent call last):
File "/ds3512/home/panyp/denovo/falcon/falcon-py2.7-ucs4/bin/fc_run.py", line 5, in
main(sys.argv)
File "/ds3512/home/panyp/ruanjian/Python2.7/lib/python2.7/site-packages/falcon_kit/mains/run1.py", line 724, in main
main1(argv[0], args.config, args.logger)
File "/ds3512/home/panyp/ruanjian/Python2.7/lib/python2.7/site-packages/falcon_kit/mains/run1.py", line 60, in main1
check_general_config(general_config, input_config_fn)
File "/ds3512/home/panyp/ruanjian/Python2.7/lib/python2.7/site-packages/falcon_kit/mains/run1.py", line 40, in check_general_config
raise Exception(msg)
Exception: Missing options.
We now require both "pa_daligner_option" (stage 0) and "ovlp_daligner_option" (stage 1),
which are automatically passed along to
HPC.daligner
HPC.TANmask
HPC.REPmask
These can provide additional flags:
pa_HPCdaligner_option
pa_HPCTANmask_option
ovlp_HPCdaligner_option
pa_REPmask_code (-g/-c pairs for 3 iterations, e.g. '1,20;5,15;20,10')
I thought my configure file had someting wrong ,here is my fc_run.cfg.
[General]
job_type = SGE
list of files of the initial bas.h5 files
input_fofn = input.fofn
#input_fofn = preads.fofn
input_type = raw
#input_type = preads
The length cutoff used for seed reads used for initial mapping
length_cutoff = 12000
The length cutoff used for seed reads usef for pre-assembly
length_cutoff_pr = 12000
jobqueue = your_queue
sge_option_da = -pe smp 8 -q %(jobqueue)s
sge_option_la = -pe smp 2 -q %(jobqueue)s
sge_option_pda = -pe smp 8 -q %(jobqueue)s
sge_option_pla = -pe smp 2 -q %(jobqueue)s
sge_option_fc = -pe smp 24 -q %(jobqueue)s
sge_option_cns = -pe smp 8 -q %(jobqueue)s
pa_concurrent_jobs = 32
ovlp_concurrent_jobs = 32
pa_HPCdaligner_option = -v -B24 -t16 -e.70 -l1000 -s1000
ovlp_HPCdaligner_option = -v -B24 -t32 -h60 -e.96 -l500 -s1000
pa_DBsplit_option = -x500 -s200
ovlp_DBsplit_option = -x500 -s200
falcon_sense_option = --output-multi --min-idt 0.70 --min-cov 4 --max-n-read 200 --n-core 6
overlap_filtering_setting = --max-diff 100 --max-cov 100 --min-cov 20 --bestn 10 --n-core 24
can you give me a favor?
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