PMID 34399627: Peroxiredoxin Asp f3 Is Essential for Aspergillus fumigatus To Overcome Iron Limitation during Infection.

[JonMWilkes]

@CuzickA - Have you come across the mitoFLARE system before? I don't know how to classify it wrt genotype - it utilises a GFP construct with mitochondrial targeting, so might be considered a delivery system for a reagent? It is appears to have no pleiotropic effects.

[CuzickA]

Curation link https://canto.phi-base.org/curs/23f6a45c5e86e878

[CuzickA]

@CuzickA - Have you come across the mitoFLARE system before? I don't know how to classify it wrt genotype - it utilises a GFP construct with mitochondrial targeting, so might be considered a delivery system for a reagent? It is appears to have no pleiotropic effects.

I haven't come across the mitoFLARE system before. GFP would usually be added as a genotype background.

[CuzickA]

Publication text
'we constructed a conditional aspf3 mutant by
replacing the endogenous promoter with a doxycycline-inducible Tet-On promoter
system (aspf3tetOn). Growth of the wild type and of the induced or repressed aspf3tetOn
mutant was indistinguishable with respect to germination, growth rate, and formation
of conidia (asexual spores) under normal growth conditions (data not shown).
However, under repressed conditions, the conditional aspf3tetOn mutant exhibited a
severe susceptibility to hydrogen peroxide on solid agar and in liquid medium (Fig. 1A
and B), very similar to a Daspf3 deletion mutant characterized in previous studies.
Induction of the conditional promoter partially rescued the increased hydrogen peroxide
susceptibility of the mutant (Fig. 1A and B).'

Next
Try and curate Fig 1D - the susceptibility of the aspf3tetOn mutant to killing by human granulocytes

[CuzickA]

UniProt id

aspf3 https://www.uniprot.org/uniprotkb/O43099/entry is a reviewed entry

[CuzickA]

I have reassigned this session to myself to continue curation.

Note, I received 2 emails an 'invitation' and a 'confirmation'.

[CuzickA]

Fig 1D legend text 'Human granulocytes (PMNs)'

[CuzickA]

'we analyzed the susceptibility of the aspf3tetOn mutant to killing induced by granulocytes
isolated from human blood (15). Surprisingly, the aspf3tetOn mutant under
repressed conditions was not more prone to granulocyte-induced killing than the wild
type (Fig. 1D).'

Tricky

1. need some new PHIPO terms for pathogen death with host (organism level in this case for fungal hyphae).
2. doesn't seem correct to add the AE infective ability 'unaffected pathogenicity' as we are not recording a disease caused in the host but instead the host response of pathogen killing.
3. also AE infects tissue does not seem quite right as this is not the tissue that the pathogen is infecting but is the host response. Maybe this needs removing and a PHIPO term needs to be developed to incorporate the 'granulocyte-induced killing' of the pathogen hyphae.

[CuzickA]

Note: This session still needs curation/re-curation.