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using Nextpolish and after polished ,produce more contigs #50

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guang-xi opened this issue Sep 2, 2020 · 4 comments
Open

using Nextpolish and after polished ,produce more contigs #50

guang-xi opened this issue Sep 2, 2020 · 4 comments

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@guang-xi
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guang-xi commented Sep 2, 2020

Hi
i used Nextpolish to polish my primary contigs fasta finished by Canu2.0. I followed the tutoral——using PE-short reads and Pacbio long reads for two rounds each.
here is my code:
########################################################
job_type = local
job_prefix = nextPolish
task = best
rewrite = yes
rerun = 3
parallel_jobs = 8
multithread_jobs = 8
genome =genome.contigs.fasta
genome_size = auto
workdir = ./01_rundir
polish_options = -p 8

[sgs_option]
sgs_fofn = ./sgs.fofn
sgs_options = -max_depth 100

[lgs_option]
lgs_fofn = ./lgs.fofn
lgs_options = -min_read_len 10k -max_read_len 150k -max_depth 60
lgs_minimap2_options = -x map-ont -t 8
########################################################
but after i polished my genome contigs ,i found that the contigs numbers increased highly,following figures are my genome.contigs.fasta、genome.nextpolish.contigs.fasta consequences statistics
image
image

could you tell me how to deal with it?

@moold
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moold commented Sep 2, 2020

what is the version of NextPolish you are using? Could you paste some seq IDs in the polished genome while not existed in the original genome?

@guang-xi
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guang-xi commented Sep 2, 2020

the version is V1.2.4

@moold
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moold commented Sep 2, 2020

I guess the short reads contain N, see FAQ vi. BTW, pls update to the latest version.

@guang-xi
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guang-xi commented Sep 2, 2020

ok,thank you ,ill try and feedback the result

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