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Hi
i used Nextpolish to polish my primary contigs fasta finished by Canu2.0. I followed the tutoral——using PE-short reads and Pacbio long reads for two rounds each.
here is my code:
########################################################
job_type = local
job_prefix = nextPolish
task = best
rewrite = yes
rerun = 3
parallel_jobs = 8
multithread_jobs = 8
genome =genome.contigs.fasta
genome_size = auto
workdir = ./01_rundir
polish_options = -p 8
[lgs_option]
lgs_fofn = ./lgs.fofn
lgs_options = -min_read_len 10k -max_read_len 150k -max_depth 60
lgs_minimap2_options = -x map-ont -t 8
########################################################
but after i polished my genome contigs ,i found that the contigs numbers increased highly,following figures are my genome.contigs.fasta、genome.nextpolish.contigs.fasta consequences statistics
could you tell me how to deal with it?
The text was updated successfully, but these errors were encountered:
Hi
i used Nextpolish to polish my primary contigs fasta finished by Canu2.0. I followed the tutoral——using PE-short reads and Pacbio long reads for two rounds each.
here is my code:
########################################################
job_type = local
job_prefix = nextPolish
task = best
rewrite = yes
rerun = 3
parallel_jobs = 8
multithread_jobs = 8
genome =genome.contigs.fasta
genome_size = auto
workdir = ./01_rundir
polish_options = -p 8
[sgs_option]
sgs_fofn = ./sgs.fofn
sgs_options = -max_depth 100
[lgs_option]
lgs_fofn = ./lgs.fofn
lgs_options = -min_read_len 10k -max_read_len 150k -max_depth 60
lgs_minimap2_options = -x map-ont -t 8
########################################################
but after i polished my genome contigs ,i found that the contigs numbers increased highly,following figures are my genome.contigs.fasta、genome.nextpolish.contigs.fasta consequences statistics
could you tell me how to deal with it?
The text was updated successfully, but these errors were encountered: