diff --git a/parameter_files/Nglyco-HCD_fragger.params b/parameter_files/Nglyco-HCD_fragger.params index ca42bac..1e5ca64 100644 --- a/parameter_files/Nglyco-HCD_fragger.params +++ b/parameter_files/Nglyco-HCD_fragger.params @@ -1,11 +1,11 @@ -# MSFragger-3.8 +# MSFragger-4.0 num_threads = 0 # Number of CPU threads to use. database_name = test.fasta # Path to the protein database file in FASTA format. precursor_mass_lower = -20 # Lower bound of the precursor mass window. precursor_mass_upper = 20 # Upper bound of the precursor mass window. precursor_mass_units = 1 # Precursor mass tolerance units (0 for Da, 1 for ppm). -data_type = 0 # Data type (0 for DDA, 1 for DIA, 2 for DIA-narrow-window). +data_type = 0 # Data type (0 for DDA, 1 for DIA, 2 for DIA-narrow-window, 3 for DDA+). precursor_true_tolerance = 20 # True precursor mass tolerance (window is +/- this value). precursor_true_units = 1 # True precursor mass tolerance units (0 for Da, 1 for ppm). fragment_mass_tolerance = 20 # Fragment mass tolerance (window is +/- this value). @@ -79,7 +79,6 @@ track_zero_topN = 0 # Track top N unmodified peptide res # set to a number greater than output_report_topN if zero bin boosting is desired. zero_bin_accept_expect = 0.00 # Ranks a zero-bin hit above all non-zero-bin hit if it has expectation less than this value. zero_bin_mult_expect = 1.00 # Multiplies expect value of PSMs in the zero-bin during results ordering (set to less than 1 for boosting). -add_topN_complementary = 0 # Inserts complementary ions corresponding to the top N most intense fragments in each experimental spectra. check_spectral_files = 1 # Checking spectral files before searching. minimum_peaks = 15 # Minimum number of peaks in experimental spectrum for matching. diff --git a/parameter_files/closed_fragger.params b/parameter_files/closed_fragger.params index 19b4fb8..2282c21 100644 --- a/parameter_files/closed_fragger.params +++ b/parameter_files/closed_fragger.params @@ -1,11 +1,11 @@ -# MSFragger-3.8 +# MSFragger-4.0 num_threads = 0 # Number of CPU threads to use. database_name = test.fasta # Path to the protein database file in FASTA format. precursor_mass_lower = -20 # Lower bound of the precursor mass window. precursor_mass_upper = 20 # Upper bound of the precursor mass window. precursor_mass_units = 1 # Precursor mass tolerance units (0 for Da, 1 for ppm). -data_type = 0 # Data type (0 for DDA, 1 for DIA, 2 for DIA-narrow-window). +data_type = 0 # Data type (0 for DDA, 1 for DIA, 2 for DIA-narrow-window, 3 for DDA+). precursor_true_tolerance = 20 # True precursor mass tolerance (window is +/- this value). precursor_true_units = 1 # True precursor mass tolerance units (0 for Da, 1 for ppm). fragment_mass_tolerance = 20 # Fragment mass tolerance (window is +/- this value). @@ -76,7 +76,6 @@ track_zero_topN = 0 # Track top N unmodified peptide res # set to a number greater than output_report_topN if zero bin boosting is desired. zero_bin_accept_expect = 0.00 # Ranks a zero-bin hit above all non-zero-bin hit if it has expectation less than this value. zero_bin_mult_expect = 1.00 # Multiplies expect value of PSMs in the zero-bin during results ordering (set to less than 1 for boosting). -add_topN_complementary = 0 # Inserts complementary ions corresponding to the top N most intense fragments in each experimental spectra. check_spectral_files = 1 # Checking spectral files before searching. minimum_peaks = 15 # Minimum number of peaks in experimental spectrum for matching. diff --git a/parameter_files/nonspecific_fragger.params b/parameter_files/nonspecific_fragger.params index 791cc6b..f9206bd 100644 --- a/parameter_files/nonspecific_fragger.params +++ b/parameter_files/nonspecific_fragger.params @@ -1,11 +1,11 @@ -# MSFragger-3.8 +# MSFragger-4.0 num_threads = 0 # Number of CPU threads to use. database_name = test.fasta # Path to the protein database file in FASTA format. precursor_mass_lower = -20 # Lower bound of the precursor mass window. precursor_mass_upper = 20 # Upper bound of the precursor mass window. precursor_mass_units = 1 # Precursor mass tolerance units (0 for Da, 1 for ppm). -data_type = 0 # Data type (0 for DDA, 1 for DIA, 2 for DIA-narrow-window). +data_type = 0 # Data type (0 for DDA, 1 for DIA, 2 for DIA-narrow-window, 3 for DDA+). precursor_true_tolerance = 20 # True precursor mass tolerance (window is +/- this value). precursor_true_units = 1 # True precursor mass tolerance units (0 for Da, 1 for ppm). fragment_mass_tolerance = 20 # Fragment mass tolerance (window is +/- this value). @@ -76,7 +76,6 @@ track_zero_topN = 0 # Track top N unmodified peptide res # set to a number greater than output_report_topN if zero bin boosting is desired. zero_bin_accept_expect = 0.00 # Ranks a zero-bin hit above all non-zero-bin hit if it has expectation less than this value. zero_bin_mult_expect = 1.00 # Multiplies expect value of PSMs in the zero-bin during results ordering (set to less than 1 for boosting). -add_topN_complementary = 0 # Inserts complementary ions corresponding to the top N most intense fragments in each experimental spectra. check_spectral_files = 1 # Checking spectral files before searching. minimum_peaks = 15 # Minimum number of peaks in experimental spectrum for matching. diff --git a/parameter_files/open_fragger.params b/parameter_files/open_fragger.params index c50b3bd..fefa4ea 100644 --- a/parameter_files/open_fragger.params +++ b/parameter_files/open_fragger.params @@ -1,11 +1,11 @@ -# MSFragger-3.8 +# MSFragger-4.0 num_threads = 0 # Number of CPU threads to use. database_name = test.fasta # Path to the protein database file in FASTA format. precursor_mass_lower = -150 # Lower bound of the precursor mass window. precursor_mass_upper = 500 # Upper bound of the precursor mass window. precursor_mass_units = 0 # Precursor mass tolerance units (0 for Da, 1 for ppm). -data_type = 0 # Data type (0 for DDA, 1 for DIA, 2 for DIA-narrow-window). +data_type = 0 # Data type (0 for DDA, 1 for DIA, 2 for DIA-narrow-window, 3 for DDA+). precursor_true_tolerance = 20 # True precursor mass tolerance (window is +/- this value). precursor_true_units = 1 # True precursor mass tolerance units (0 for Da, 1 for ppm). fragment_mass_tolerance = 20 # Fragment mass tolerance (window is +/- this value). @@ -76,7 +76,6 @@ track_zero_topN = 0 # Track top N unmodified peptide res # set to a number greater than output_report_topN if zero bin boosting is desired. zero_bin_accept_expect = 0.00 # Ranks a zero-bin hit above all non-zero-bin hit if it has expectation less than this value. zero_bin_mult_expect = 1.00 # Multiplies expect value of PSMs in the zero-bin during results ordering (set to less than 1 for boosting). -add_topN_complementary = 0 # Inserts complementary ions corresponding to the top N most intense fragments in each experimental spectra. check_spectral_files = 1 # Checking spectral files before searching. minimum_peaks = 15 # Minimum number of peaks in experimental spectrum for matching.