diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 94c8b3e..47a403b 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -10,7 +10,7 @@ repos: # pre-commit's default_language_version, see # https://pre-commit.com/#top_level-default_language_version language_version: python3.11 - + files: ^(src|test)/.*\.py$ - repo: https://github.com/pycqa/isort rev: 5.13.2 @@ -18,3 +18,4 @@ repos: - id: isort args: ["--profile", "black"] name: isort (python) + files: ^(src|test)/.*\.py$ diff --git a/docs/examples/new_sample.py b/docs/examples/new_sample.py index ebf7144..6f7b2c9 100644 --- a/docs/examples/new_sample.py +++ b/docs/examples/new_sample.py @@ -3,14 +3,15 @@ See official documentation at https://scikit-image.org/docs/0.7.0/api/skimage.transform.html#probabilistic-hough The procedure to follow is, in short: -- import everything from the samples module +- import everything from the analyses module - import the types that you might be using from the typing module - import any necessary libraries that you will need for your analysis -- Create one or more subclasses of the Analysis abstract class of samples. Within each class: +- Create one or more subclasses of the Analysis abstract class of analyses. Within each class: - define your input requirements - define a 'run' method that will implement the logic of your analysis - if desired, define a 'plot' method returning a plot showing the results of the analysis """ + from math import atan2 # import the types that you may be using @@ -25,7 +26,7 @@ from skimage.transform import probabilistic_hough_line # import the sample functionality -from microscopemetrics.samples import * +from microscopemetrics.analyses import * class DetectLinesAnalysis( diff --git a/docs/notebooks/microscope-metrics_tutorial.ipynb b/docs/notebooks/microscope-metrics_tutorial.ipynb index d18f017..49e4967 100644 --- a/docs/notebooks/microscope-metrics_tutorial.ipynb +++ b/docs/notebooks/microscope-metrics_tutorial.ipynb @@ -805,4 +805,4 @@ }, "nbformat": 4, "nbformat_minor": 1 -} \ No newline at end of file +} diff --git a/poetry.lock b/poetry.lock index 8cf6df6..8191f62 100644 --- a/poetry.lock +++ b/poetry.lock @@ -1,4 +1,4 @@ -# This file is automatically @generated by Poetry 1.8.4 and should not be changed by hand. +# This file is automatically @generated by Poetry 1.8.3 and should not be changed by hand. 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"Jupyter interactive widgets for Jupyter Notebook" +optional = false +python-versions = ">=3.7" +files = [ + {file = "widgetsnbextension-4.0.13-py3-none-any.whl", hash = "sha256:74b2692e8500525cc38c2b877236ba51d34541e6385eeed5aec15a70f88a6c71"}, + {file = "widgetsnbextension-4.0.13.tar.gz", hash = "sha256:ffcb67bc9febd10234a362795f643927f4e0c05d9342c727b65d2384f8feacb6"}, +] + [[package]] name = "wrapt" version = "1.16.0" @@ -2640,5 +4586,5 @@ type = ["pytest-mypy"] [metadata] lock-version = "2.0" -python-versions = ">=3.10.12 <3.12" -content-hash = "2bf8c80a808841bc3b35049546502caf15bc227e646ef1cdff02768d81bcc575" +python-versions = ">=3.9.18 <3.12" +content-hash = "bc485ecf1a3aa6a180f8a8beb1b1abbd118297e43203cb48b72af7434ec35b5c" diff --git a/pyproject.toml b/pyproject.toml index ca494c0..d46e61b 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -14,7 +14,7 @@ documentation = "https://github.com/MontpellierRessourcesImagerie/microscope-met keywords = ["microscopy", "metrology"] [tool.pytest.ini_options] -addopts = "--hypothesis-show-statistics --hypothesis-profile=dev --workers=auto" +addopts = "--hypothesis-show-statistics --hypothesis-profile=dev" # --workers=auto" testpaths = [ "tests" ] @@ -30,13 +30,17 @@ filterwarnings = [ "ignore:.*urllib3 v2 only supports OpenSSL.*:Warning", ] +[tool.isort] +profile = "black" + [tool.poetry.dependencies] -python = ">=3.10.12 <3.12" +python = ">=3.9.18 <3.12" numpy = "^1" scikit-image = "^0" scipy = "^1" pandas = "^1" -microscopemetrics-schema = {git = "https://github.com/juliomateoslangerak/microscopemetrics-schema.git"} +matplotlib = "^3" +microscopemetrics-schema = {git = "https://github.com/juliomateoslangerak/microscopemetrics-schema.git", branch = "dev"} [tool.poetry.group.test.dependencies] pytest = "^7" @@ -48,6 +52,7 @@ pytest-parallel = "^0.1.1" [tool.poetry.group.dev.dependencies] black = "^23" poetry = "^1" +jupyter = "^1" isort = "^5" pre-commit = "^3" diff --git a/src/microscopemetrics/samples/__init__.py b/src/microscopemetrics/analyses/__init__.py similarity index 63% rename from src/microscopemetrics/samples/__init__.py rename to src/microscopemetrics/analyses/__init__.py index c204c02..828d547 100644 --- a/src/microscopemetrics/samples/__init__.py +++ b/src/microscopemetrics/analyses/__init__.py @@ -1,4 +1,4 @@ -# Main samples module defining the sample superclass +# Main analyses module defining the sample superclass import logging from abc import ABC, abstractmethod from datetime import datetime @@ -8,51 +8,8 @@ import numpy as np import pandas as pd -# We are defining some global dictionaries to register the different analysis types -IMAGE_ANALYSIS_REGISTRY = {} -DATASET_ANALYSIS_REGISTRY = {} -PROGRESSION_ANALYSIS_REGISTRY = {} - - -# Decorators to register exposed analysis functions -def register_image_analysis(cls): - IMAGE_ANALYSIS_REGISTRY[cls.__name__] = cls - return cls - - -def register_dataset_analysis(cls): - DATASET_ANALYSIS_REGISTRY[cls.__name__] = cls - return cls - - -def register_progression_analysis(cls): - PROGRESSION_ANALYSIS_REGISTRY[cls.__name__] = cls - return cls - - # Create a logging service logger = logging.getLogger(__name__) -# TODO: work on the loggers - - -# def get_references( -# objects: Union[mm_schema.MetricsObject, list[mm_schema.MetricsObject]] -# ) -> List[mm_schema.DataReference]: -# """Get the references of a metrics object or a list of metrics objects""" -# if isinstance(objects, mm_schema.MetricsObject): -# return mm_schema.DataReference( -# data_uri=objects.data_uri, -# # HACK: This is a temporary fix to get the first element of the tuple -# omero_host=objects.omero_host[0], -# omero_port=objects.omero_port[0], -# omero_object_type=objects.omero_object_type[0], -# omero_object_id=objects.omero_object_id, -# ) -# -# elif isinstance(objects, list): -# return [get_references(obj) for obj in objects] -# else: -# raise ValueError("Input should be a metrics object or a list of metrics objects") def get_object_id( @@ -152,72 +109,6 @@ def numpy_to_mm_image( ) -# def numpy_to_mask_inlined( -# array: np.ndarray, -# name: str = None, -# description: str = None, -# image_url: str = None, -# source_image_url: Union[str, List[str]] = None, -# ) -> mm_schema.ImageMask: -# """Converts a bool numpy array with dimensions order yx to an inlined mask""" -# if array.ndim != 2: -# raise ValueError("Input array should be 2D") -# if array.dtype != bool and array.dtype == np.uint8: -# try: -# array = array.astype(bool) -# except ValueError: -# raise ValueError("Input array could not be casted to type bool") -# if array.dtype != bool: -# raise ValueError("Input array should be of type bool") -# -# return mm_schema.ImageMask( -# name=name, -# description=description, -# image_url=image_url, -# source_image_url=source_image_url, -# data=array.flatten().tolist(), -# shape_y=array.shape[0], -# shape_x=array.shape[1], -# ) -# -# -# def numpy_to_image_inlined( -# array: np.ndarray, -# name: str = None, -# description: str = None, -# image_url: str = None, -# source_image_url: Union[str, List[str]] = None, -# ) -> mm_schema.ImageInline: -# """Converts a numpy array with dimensions order tzyxc or yx to an inlined image""" -# if array.ndim == 5: -# return mm_schema.Image5D( -# name=name, -# description=description, -# image_url=image_url, -# source_image_url=source_image_url, -# data=array.flatten().tolist(), -# shape_t=array.shape[0], -# shape_z=array.shape[1], -# shape_y=array.shape[2], -# shape_x=array.shape[3], -# shape_c=array.shape[4], -# ) -# elif array.ndim == 2: -# return mm_schema.Image2D( -# name=name, -# description=description, -# image_url=image_url, -# source_image_url=source_image_url, -# data=array.flatten().tolist(), -# shape_y=array.shape[0], -# shape_x=array.shape[1], -# ) -# else: -# raise NotImplementedError( -# f"Array of dimension {array.ndim} is not supported by this function" -# ) - - def _create_table( data: Union[dict[str, list], pd.DataFrame], name: str, diff --git a/src/microscopemetrics/samples/argolight.py b/src/microscopemetrics/analyses/argolight.py similarity index 89% rename from src/microscopemetrics/samples/argolight.py rename to src/microscopemetrics/analyses/argolight.py index 7104884..d5ad191 100644 --- a/src/microscopemetrics/samples/argolight.py +++ b/src/microscopemetrics/analyses/argolight.py @@ -1,24 +1,25 @@ from datetime import datetime from itertools import product -from typing import Any, Dict, List, Tuple, Union +from typing import Dict, List, Tuple import microscopemetrics_schema.datamodel as mm_schema import numpy as np import pandas as pd from numpy import float64, int64, ndarray from pandas import DataFrame -from scipy.interpolate import griddata from scipy.optimize import curve_fit from scipy.signal import find_peaks from skimage.transform import hough_line # hough_line_peaks, probabilistic_hough_line -from microscopemetrics.analysis.tools import ( +from microscopemetrics.analyses import logger, numpy_to_mm_image, validate_requirements +from microscopemetrics.analyses.tools import ( + airy_fun, compute_distances_matrix, compute_spots_properties, + is_saturated, + multi_airy_fun, segment_image, ) -from microscopemetrics.samples import logger, numpy_to_mm_image, validate_requirements -from microscopemetrics.utilities.utilities import airy_fun, is_saturated, multi_airy_fun def _profile_to_columns(profile: ndarray, channel: int) -> List[Dict[str, Dict[str, List[float]]]]: @@ -223,12 +224,12 @@ def _compute_resolution( ) -def analise_argolight_b(dataset: mm_schema.ArgolightBDataset) -> bool: +def analyse_argolight_b(dataset: mm_schema.ArgolightBDataset) -> bool: validate_requirements() # Check image shape logger.info("Checking image shape...") - image = dataset.input.argolight_b_image.data + image = dataset.input_data.argolight_b_image.data if len(image.shape) != 5: logger.error("Image must be 5D") return False @@ -239,8 +240,8 @@ def analise_argolight_b(dataset: mm_schema.ArgolightBDataset) -> bool: for c in range(image.shape[-1]): if is_saturated( channel=image[:, :, :, :, c], - threshold=dataset.input.saturation_threshold, - detector_bit_depth=dataset.input.bit_depth, + threshold=dataset.input_parameters.saturation_threshold, + detector_bit_depth=dataset.input_parameters.bit_depth, ): logger.error(f"Channel {c} is saturated") saturated_channels.append(c) @@ -249,32 +250,36 @@ def analise_argolight_b(dataset: mm_schema.ArgolightBDataset) -> bool: return False # Calculating the distance between spots in pixels with a security margin - min_distance = round(dataset.input.spots_distance * 0.3) + min_distance = round(dataset.input_parameters.spots_distance * 0.3) # Calculating the maximum tolerated distance in microns for the same spot in a different channels - max_distance = dataset.input.spots_distance * 0.4 + max_distance = dataset.input_parameters.spots_distance * 0.4 labels = segment_image( image=image, min_distance=min_distance, - sigma=(dataset.input.sigma_z, dataset.input.sigma_y, dataset.input.sigma_x), + sigma=( + dataset.input_parameters.sigma_z, + dataset.input_parameters.sigma_y, + dataset.input_parameters.sigma_x, + ), method="local_max", - low_corr_factors=dataset.input.lower_threshold_correction_factors, - high_corr_factors=dataset.input.upper_threshold_correction_factors, + low_corr_factors=dataset.input_parameters.lower_threshold_correction_factors, + high_corr_factors=dataset.input_parameters.upper_threshold_correction_factors, ) dataset.output.spots_labels_image = numpy_to_mm_image( # TODO: this should be a mask array=labels, - name=f"{dataset.input.argolight_b_image.name}_spots_labels", - description=f"Spots labels of {dataset.input.argolight_b_image.image_url}", - image_url=dataset.input.argolight_b_image.image_url, - source_image_url=dataset.input.argolight_b_image.image_url, + name=f"{dataset.input_data.argolight_b_image.name}_spots_labels", + description=f"Spots labels of {dataset.input_data.argolight_b_image.image_url}", + image_url=dataset.input_data.argolight_b_image.image_url, + source_image_url=dataset.input_data.argolight_b_image.image_url, ) spots_properties, spots_positions = compute_spots_properties( image=image, labels=labels, - remove_center_cross=dataset.input.remove_center_cross, + remove_center_cross=dataset.input_parameters.remove_center_cross, ) distances_df = compute_distances_matrix( @@ -338,7 +343,7 @@ def analise_argolight_b(dataset: mm_schema.ArgolightBDataset) -> bool: spots_centroids.append( mm_schema.Roi( label=f"Centroids_ch{ch:02}", - image=dataset.input.argolight_b_image.image_url, + image=dataset.input_data.argolight_b_image.image_url, shapes=channel_shapes, ) ) @@ -398,7 +403,7 @@ def analise_argolight_b(dataset: mm_schema.ArgolightBDataset) -> bool: return True -def analise_argolight_e(dataset: mm_schema.ArgolightEDataset) -> bool: +def analyse_argolight_e(dataset: mm_schema.ArgolightEDataset) -> bool: validate_requirements() # Check image shape @@ -410,11 +415,11 @@ def analise_argolight_e(dataset: mm_schema.ArgolightEDataset) -> bool: # Check for axis value pass # TODO: implement - image = dataset.input.argolight_e_image.data + image = dataset.input_data.argolight_e_image.data image = image[0] # if there is a time dimension, take the first one - axis = dataset.input.orientation_axis - pixel_size = dataset.input.argolight_e_image.voxel_size_x_micron - measured_band = dataset.input.measured_band + axis = dataset.input_parameters.orientation_axis + pixel_size = dataset.input_data.argolight_e_image.voxel_size_x_micron + measured_band = dataset.input_parameters.measured_band ( profiles, @@ -428,7 +433,7 @@ def analise_argolight_e(dataset: mm_schema.ArgolightEDataset) -> bool: image=image, axis=axis, measured_band=measured_band, - prominence=dataset.input.prominence_threshold, + prominence=dataset.input_parameters.prominence_threshold, do_angle_refinement=False, # TODO: implement angle refinement ) key_values = { @@ -498,7 +503,7 @@ def analise_argolight_e(dataset: mm_schema.ArgolightEDataset) -> bool: rois.append( mm_schema.Roi( label=f"ch{ch:02}_peaks", - image=dataset.input.argolight_e_image.image_url, + image=dataset.input_data.argolight_e_image.image_url, shapes=shapes, ) ) diff --git a/src/microscopemetrics/samples/field_illumination.py b/src/microscopemetrics/analyses/field_illumination.py similarity index 95% rename from src/microscopemetrics/samples/field_illumination.py rename to src/microscopemetrics/analyses/field_illumination.py index 53fe44c..5f7f373 100644 --- a/src/microscopemetrics/samples/field_illumination.py +++ b/src/microscopemetrics/analyses/field_illumination.py @@ -11,14 +11,14 @@ from skimage.measure import regionprops from microscopemetrics import SaturationError -from microscopemetrics.samples import ( +from microscopemetrics.analyses import ( dict_to_table, get_object_id, logger, numpy_to_mm_image, validate_requirements, ) -from microscopemetrics.utilities.utilities import fit_gaussian, is_saturated +from microscopemetrics.analyses.tools import fit_gaussian, is_saturated def _get_center_region_mask(channel: np.ndarray, fraction: float = 0.1) -> np.ndarray: @@ -341,11 +341,11 @@ def _image_properties(images: list[mm_schema.Image], corner_fraction: float, sig return properties -def analise_field_illumination(dataset: mm_schema.FieldIlluminationDataset) -> bool: +def analyse_field_illumination(dataset: mm_schema.FieldIlluminationDataset) -> bool: validate_requirements() channel_names = [] - for image in dataset.input.field_illumination_image: + for image in dataset.input_data.field_illumination_image: # We want to verify that the input images all have different channel names # As it does not make sense to average file illumination between images from the same channel if image.channel_series is not None: @@ -375,8 +375,8 @@ def analise_field_illumination(dataset: mm_schema.FieldIlluminationDataset) -> b for c in range(image.array_data.shape[-1]): if is_saturated( channel=image.array_data[..., c], - threshold=dataset.input.saturation_threshold, - detector_bit_depth=dataset.input.bit_depth, + threshold=dataset.input_parameters.saturation_threshold, + detector_bit_depth=dataset.input_parameters.bit_depth, ): logger.error(f"Channel {c} is saturated") saturated_channels.append(c) @@ -385,9 +385,9 @@ def analise_field_illumination(dataset: mm_schema.FieldIlluminationDataset) -> b raise SaturationError(f"Channels {saturated_channels} are saturated") key_measurements = _image_properties( - images=dataset.input.field_illumination_image, - corner_fraction=dataset.input.corner_fraction, - sigma=dataset.input.sigma, + images=dataset.input_data.field_illumination_image, + corner_fraction=dataset.input_parameters.corner_fraction, + sigma=dataset.input_parameters.sigma, ) key_measurements = mm_schema.FieldIlluminationKeyMeasurements( @@ -403,7 +403,7 @@ def analise_field_illumination(dataset: mm_schema.FieldIlluminationDataset) -> b name=f"{image.name}_intensity_profiles", description=f"Intensity profiles of {image.name}", ) - for image in dataset.input.field_illumination_image + for image in dataset.input_data.field_illumination_image ] roi_profiles = [ @@ -413,7 +413,7 @@ def analise_field_illumination(dataset: mm_schema.FieldIlluminationDataset) -> b linked_references=image.data_reference, lines=_line_profile_shapes(image.array_data), ) - for image in dataset.input.field_illumination_image + for image in dataset.input_data.field_illumination_image ] roi_corners = mm_schema.Roi( @@ -421,10 +421,10 @@ def analise_field_illumination(dataset: mm_schema.FieldIlluminationDataset) -> b description="ROIs used to compute the corner intensities", linked_references=[ i.data_reference - for i in dataset.input.field_illumination_image + for i in dataset.input_data.field_illumination_image if i.data_reference is not None ], - rectangles=_corner_shapes(image.array_data, dataset.input.corner_fraction), + rectangles=_corner_shapes(image.array_data, dataset.input_parameters.corner_fraction), ) roi_centers_of_mass = [ @@ -449,7 +449,7 @@ def analise_field_illumination(dataset: mm_schema.FieldIlluminationDataset) -> b for c in range(image.array_data.shape[-1]) ], ) - for image in dataset.input.field_illumination_image + for image in dataset.input_data.field_illumination_image ] roi_centers_geometric = [ @@ -474,7 +474,7 @@ def analise_field_illumination(dataset: mm_schema.FieldIlluminationDataset) -> b for c in range(image.array_data.shape[-1]) ], ) - for image in dataset.input.field_illumination_image + for image in dataset.input_data.field_illumination_image ] roi_centers_fitted = [ @@ -499,7 +499,7 @@ def analise_field_illumination(dataset: mm_schema.FieldIlluminationDataset) -> b for c in range(image.array_data.shape[-1]) ], ) - for image in dataset.input.field_illumination_image + for image in dataset.input_data.field_illumination_image ] roi_centers_max_intensity = [ @@ -524,7 +524,7 @@ def analise_field_illumination(dataset: mm_schema.FieldIlluminationDataset) -> b for c in range(image.array_data.shape[-1]) ], ) - for image in dataset.input.field_illumination_image + for image in dataset.input_data.field_illumination_image ] roi_center_region = [ @@ -546,7 +546,7 @@ def analise_field_illumination(dataset: mm_schema.FieldIlluminationDataset) -> b for c in range(image.array_data.shape[-1]) ], ) - for image in dataset.input.field_illumination_image + for image in dataset.input_data.field_illumination_image ] dataset.output = mm_schema.FieldIlluminationOutput( diff --git a/src/microscopemetrics/analyses/mappings.py b/src/microscopemetrics/analyses/mappings.py new file mode 100644 index 0000000..e93b26d --- /dev/null +++ b/src/microscopemetrics/analyses/mappings.py @@ -0,0 +1,47 @@ +""" +This module maps the sample classes to the analysis functions and the dataset classes. +""" + +from collections import namedtuple + +from microscopemetrics_schema import datamodel as mm_schema + +from microscopemetrics.analyses import field_illumination, psf_beads + +Mapping = namedtuple("Mapping", ["sample_class", "analysis_function", "dataset_class"]) + +MAPPINGS = [ + Mapping( + mm_schema.FluorescentHomogeneousThinField, + field_illumination.analyse_field_illumination, + mm_schema.FieldIlluminationDataset, + ), + Mapping( + mm_schema.FluorescentHomogeneousThickField, + field_illumination.analyse_field_illumination, + mm_schema.FieldIlluminationDataset, + ), + Mapping(mm_schema.PSFBeads, psf_beads.analyse_psf_beads, mm_schema.PSFBeadsDataset), +] + +# TEST = { +# mm_schema.FieldIlluminationDataset: { +# "sample_class": [ +# mm_schema.FluorescentHomogeneousThinField, +# mm_schema.FluorescentHomogeneousThickField, +# ], +# "analysis_function": [ +# field_illumination.analyse_field_illumination, +# field_illumination.analyse_field_illumination, +# ], +# }, +# mm_schema.PSFBeadsDataset: { +# "sample_class": [ +# mm_schema.PSFBeads, +# ], +# "analysis_function": [ +# psf_beads.analyse_psf_beads, +# ], +# }, +# } +# diff --git a/src/microscopemetrics/samples/psf_beads.py b/src/microscopemetrics/analyses/psf_beads.py similarity index 96% rename from src/microscopemetrics/samples/psf_beads.py rename to src/microscopemetrics/analyses/psf_beads.py index 0bd13da..da5d585 100644 --- a/src/microscopemetrics/samples/psf_beads.py +++ b/src/microscopemetrics/analyses/psf_beads.py @@ -8,14 +8,14 @@ from skimage.filters import gaussian from microscopemetrics import FittingError, SaturationError -from microscopemetrics.samples import ( +from microscopemetrics.analyses import ( df_to_table, get_object_id, logger, numpy_to_mm_image, validate_requirements, ) -from microscopemetrics.utilities.utilities import fit_airy, fit_gaussian, is_saturated +from microscopemetrics.analyses.tools import fit_airy, fit_gaussian, is_saturated def _add_column_name_level(df: pd.DataFrame, level_name: str, level_value: str): @@ -203,7 +203,7 @@ def _generate_key_measurements(bead_properties, average_bead_properties): # We aggregate counts for each channel on beads according to their status channel_counts = ( reindex_bead_properties_df[count_aggregation_columns] - .groupby(["channel_name", "channel_nr"]) + .groupby(["channel_nr", "channel_name"]) .agg(["sum"]) ) channel_counts.columns = [ @@ -216,7 +216,7 @@ def _generate_key_measurements(bead_properties, average_bead_properties): ] channel_measurements = ( valid_bead_properties_df[measurement_aggregation_columns] - .groupby(["channel_name", "channel_nr"]) + .groupby(["channel_nr", "channel_name"]) .agg(["mean", "median", "std"]) ) channel_measurements.columns = [ @@ -511,7 +511,7 @@ def _process_image( def _estimate_min_bead_distance(dataset: mm_schema.PSFBeadsDataset) -> float: # TODO: get the resolution somewhere or pass it as a metadata - return dataset.input.min_lateral_distance_factor + return dataset.input_parameters.min_lateral_distance_factor def _generate_center_roi( @@ -523,7 +523,7 @@ def _generate_center_roi( ): rois = [] - for image in dataset.input.psf_beads_images: + for image in dataset.input_data.psf_beads_images: image_id = get_object_id(image) or image.name if positions.empty or image_id not in positions.index.get_level_values("image_id"): continue @@ -566,7 +566,7 @@ def _extract_profiles(bead_properties, axis: str) -> pd.DataFrame: profiles = {} for index, row in bead_properties.iterrows(): if isinstance(index, (list, tuple)): - index = {index[bead_properties.index.names.index(col_i)] for col_i in column_indexes} + index = [index[bead_properties.index.names.index(col_i)] for col_i in column_indexes] index_str = "_".join([str(i) for i in index]) else: @@ -587,15 +587,15 @@ def analyse_psf_beads(dataset: mm_schema.PSFBeadsDataset) -> bool: images = {} voxel_sizes_micron = {} min_bead_distance = _estimate_min_bead_distance(dataset) - snr_threshold = dataset.input.snr_threshold - fitting_r2_threshold = dataset.input.fitting_r2_threshold + snr_threshold = dataset.input_parameters.snr_threshold + fitting_r2_threshold = dataset.input_parameters.fitting_r2_threshold # Containers for output data saturated_channels = {} bead_properties = [] # First loop to prepare data - for image in dataset.input.psf_beads_images: + for image in dataset.input_data.psf_beads_images: image_id = get_object_id(image) or image.name images[image_id] = image.array_data[0, ...] @@ -616,8 +616,8 @@ def analyse_psf_beads(dataset: mm_schema.PSFBeadsDataset) -> bool: for c in range(image.array_data.shape[-1]): if is_saturated( channel=image.array_data[..., c], - threshold=dataset.input.saturation_threshold, - detector_bit_depth=dataset.input.bit_depth, + threshold=dataset.input_parameters.saturation_threshold, + detector_bit_depth=dataset.input_parameters.bit_depth, ): logger.error(f"Image {image_id}: channel {c} is saturated") saturated_channels[image_id].append(c) @@ -627,7 +627,7 @@ def analyse_psf_beads(dataset: mm_schema.PSFBeadsDataset) -> bool: raise SaturationError(f"Channels {saturated_channels} are saturated") # Second loop main image analysis - for image in dataset.input.psf_beads_images: + for image in dataset.input_data.psf_beads_images: image_id = get_object_id(image) or image.name logger.info(f"Processing image {image_id}...") voxel_sizes_micron[image_id] = ( @@ -638,11 +638,15 @@ def analyse_psf_beads(dataset: mm_schema.PSFBeadsDataset) -> bool: image_bead_properties = _process_image( image=image, - sigma=(dataset.input.sigma_z, dataset.input.sigma_y, dataset.input.sigma_x), + sigma=( + dataset.input_parameters.sigma_z, + dataset.input_parameters.sigma_y, + dataset.input_parameters.sigma_x, + ), min_bead_distance=min_bead_distance, snr_threshold=snr_threshold, fitting_r2_threshold=fitting_r2_threshold, - intensity_robust_z_score_threshold=dataset.input.intensity_robust_z_score_threshold, + intensity_robust_z_score_threshold=dataset.input_parameters.intensity_robust_z_score_threshold, ) logger.info( @@ -664,7 +668,7 @@ def analyse_psf_beads(dataset: mm_schema.PSFBeadsDataset) -> bool: # Before averaging any bead we need to verify that all voxel sizes are equal if len(set(voxel_sizes_micron.values())) == 1: - average_beads_properties = bead_properties.groupby(["channel_name", "channel_nr"]).apply( + average_beads_properties = bead_properties.groupby(["channel_nr", "channel_name"]).apply( _average_beads ) average_beads_properties = average_beads_properties.join( @@ -701,7 +705,7 @@ def analyse_psf_beads(dataset: mm_schema.PSFBeadsDataset) -> bool: ), name="average_bead", description="Average bead image extracted from all the beads considered valid in the dataset.", - source_images=dataset.input.psf_beads_images, + source_images=dataset.input_data.psf_beads_images, channel_names=[ i[average_beads_properties.index.names.index("channel_name")] for i in average_beads_properties.index diff --git a/src/microscopemetrics/analysis/tools.py b/src/microscopemetrics/analyses/tools.py similarity index 53% rename from src/microscopemetrics/analysis/tools.py rename to src/microscopemetrics/analyses/tools.py index e75d290..1794767 100644 --- a/src/microscopemetrics/analysis/tools.py +++ b/src/microscopemetrics/analyses/tools.py @@ -1,11 +1,13 @@ """These are some possibly useful code snippets""" + import logging from itertools import permutations -from typing import List +from typing import List, Optional import numpy as np import pandas as pd -from scipy import ndimage +from scipy import ndimage, special +from scipy.optimize import curve_fit, fsolve from scipy.spatial.distance import cdist from skimage.feature import peak_local_max from skimage.filters import apply_hysteresis_threshold, gaussian, threshold_otsu @@ -16,6 +18,156 @@ # Creating logging services logger = logging.getLogger(__name__) +INT_DETECTOR_BIT_DEPTHS = [8, 10, 11, 12, 15, 16, 32] +FLOAT_DETECTOR_BIT_DEPTHS = [32, 64] + + +def airy_fun( + x: np.ndarray, centre: np.float64, amp: np.float64 +) -> np.ndarray: # , exp): # , amp, bg): + with np.errstate(divide="ignore", invalid="ignore"): + return np.where( + (x - centre) == 0, + amp * 0.5**2, + amp * (special.j1(x - centre) / (x - centre)) ** 2, + ) + + +def gaussian_fun(x, background, amplitude, center, sd): + gauss = np.exp(-np.power(x - center, 2.0) / (2 * np.power(sd, 2.0))) + return background + (amplitude - background) * gauss + + +def fit_gaussian(profile, guess=None): + if guess is None: + guess = [profile.min(), profile.max(), profile.argmax(), 0.8] + x = np.linspace(0, profile.shape[0], profile.shape[0], endpoint=False) + popt, pcov, infodict, mesg, ier = curve_fit( + f=gaussian_fun, xdata=x, ydata=profile, p0=guess, maxfev=5000, full_output=True + ) + + if ier not in [1, 2, 3, 4]: + logger.warning(f"No gaussian fit found. Reason: {mesg}") + + fitted_profile = gaussian_fun(x, popt[0], popt[1], popt[2], popt[3]) + fwhm = popt[3] * 2.35482 + + # Calculate the fit quality using the coefficient of determination (R^2) + y_mean = np.mean(profile) + sst = np.sum((profile - y_mean) ** 2) + ssr = np.sum((profile - fitted_profile) ** 2) + cd_r2 = 1 - (ssr / sst) + + return fitted_profile, cd_r2, fwhm, popt + + +def fit_airy(profile, guess=None): + profile = (profile - profile.min()) / (profile.max() - profile.min()) + if guess is None: + guess = [profile.argmax(), 4 * profile.max()] + x = np.linspace(0, profile.shape[0], profile.shape[0], endpoint=False) + popt, pcov, infodict, mesg, ier = curve_fit( + f=airy_fun, xdata=x, ydata=profile, p0=guess, full_output=True + ) + + if ier not in [1, 2, 3, 4]: + logger.warning(f"No airy fit found. Reason: {mesg}") + + fitted_profile = airy_fun(x, popt[0], popt[1]) + + # Calculate the FWHM + def _f(d): + return airy_fun(d, popt[0], popt[1]) - (fitted_profile.max() - fitted_profile.min()) / 2 + + guess = np.array([fitted_profile.argmax() - 1, fitted_profile.argmax() + 1]) + v = fsolve(_f, guess) + fwhm = abs(v[1] - v[0]) + + # Calculate the fit quality using the coefficient of determination (R^2) + y_mean = np.mean(profile) + sst = np.sum((profile - y_mean) ** 2) + ssr = np.sum((profile - fitted_profile) ** 2) + cd_r2 = 1 - (ssr / sst) + + return fitted_profile, cd_r2, fwhm, popt + + +def multi_airy_fun(x: np.ndarray, *params) -> np.ndarray: + y = np.zeros_like(x) + for i in range(0, len(params), 2): + y = y + airy_fun(x, params[i], params[i + 1]) + return y + + +def is_saturated( + channel: np.ndarray, threshold: float = 0.0, detector_bit_depth: Optional[int] = None +) -> bool: + """ + Checks if the channel is saturated. + A warning if it suspects that the detector bit depth does not match the datatype. + thresh: float + Threshold for the ratio of saturated pixels to total pixels + detector_bit_depth: int + Bit depth of the detector. Sometimes, detectors bit depth are not matching the datatype of the measureemnts. + Here it can be specified the bit depth of the detector if known. The function is going to raise + If None, it will be inferred from the channel dtype. + """ + if detector_bit_depth is not None: + if ( + detector_bit_depth not in INT_DETECTOR_BIT_DEPTHS + and detector_bit_depth not in FLOAT_DETECTOR_BIT_DEPTHS + ): + raise ValueError( + f"The detector bit depth provided ({detector_bit_depth}) is not supported. Supported values are {INT_DETECTOR_BIT_DEPTHS} for integer detectors and {FLOAT_DETECTOR_BIT_DEPTHS} for floating point detectors." + ) + if ( + np.issubdtype(channel.dtype, np.integer) + and detector_bit_depth not in INT_DETECTOR_BIT_DEPTHS + ): + raise ValueError( + f"The channel datatype {channel.dtype} does not match the detector bit depth {detector_bit_depth}. The channel might be saturated." + ) + elif ( + np.issubdtype(channel.dtype, np.floating) + and detector_bit_depth not in FLOAT_DETECTOR_BIT_DEPTHS + ): + raise ValueError( + f"The channel datatype {channel.dtype} does not match the detector bit depth {detector_bit_depth}. The channel might be saturated." + ) + else: + if np.issubdtype(channel.dtype, np.integer): + if detector_bit_depth > np.iinfo(channel.dtype).bits: + raise ValueError( + f"The channel datatype {channel.dtype} does not support the detector bit depth {detector_bit_depth}. The channel might be saturated." + ) + else: + max_limit = pow(2, detector_bit_depth) - 1 + elif np.issubdtype(channel.dtype, np.floating): + if detector_bit_depth != np.finfo(channel.dtype).bits: + raise ValueError( + f"The channel datatype {channel.dtype} does not support the detector bit depth {detector_bit_depth}. The channel might be saturated." + ) + else: + max_limit = np.finfo(channel.dtype).max + + else: + if np.issubdtype(channel.dtype, np.integer): + max_limit = np.iinfo(channel.dtype).max + elif np.issubdtype(channel.dtype, np.floating): + max_limit = np.finfo(channel.dtype).max + else: + raise ValueError("The channel provided is not a valid numpy dtype.") + + if channel.max() > max_limit: + raise ValueError( + "The channel provided has values larger than the bit depth of the detector." + ) + + saturation_matrix = channel == max_limit + saturation_ratio = np.count_nonzero(saturation_matrix) / channel.size + + return saturation_ratio > threshold + def _segment_channel( channel, @@ -257,3 +409,52 @@ def fft_3d(image): fft[..., c, :, :] = _channel_fft_3d(image[..., c, :, :]) return fft + + +def wavelength_to_rgb(wavelength, gamma=0.8): + """ + Copied from https://www.noah.org/wiki/Wavelength_to_RGB_in_Python + This converts a given wavelength of light to an + approximate RGB color value. The wavelength must be given + in nanometers in the range from 380 nm through 750 nm + (789 THz through 400 THz). + + Based on code by Dan Bruton + http://www.physics.sfasu.edu/astro/color/spectra.html + """ + + wavelength = float(wavelength) + if 380 < wavelength <= 440: + attenuation = 0.3 + 0.7 * (wavelength - 380) / (440 - 380) + r = ((-(wavelength - 440) / (440 - 380)) * attenuation) ** gamma + g = 0.0 + b = (1.0 * attenuation) ** gamma + elif 440 < wavelength <= 490: + r = 0.0 + g = ((wavelength - 440) / (490 - 440)) ** gamma + b = 1.0 + elif 490 < wavelength <= 510: + r = 0.0 + g = 1.0 + b = (-(wavelength - 510) / (510 - 490)) ** gamma + elif 510 < wavelength <= 580: + r = ((wavelength - 510) / (580 - 510)) ** gamma + g = 1.0 + b = 0.0 + elif 580 < wavelength <= 645: + r = 1.0 + g = (-(wavelength - 645) / (645 - 580)) ** gamma + b = 0.0 + elif 645 < wavelength <= 750: + attenuation = 0.3 + 0.7 * (750 - wavelength) / (750 - 645) + r = (1.0 * attenuation) ** gamma + g = 0.0 + b = 0.0 + else: + r = 0.0 + g = 0.0 + b = 0.0 + r *= int(r * 255) + g *= int(g * 255) + g *= int(g * 255) + return r, g, b diff --git a/src/microscopemetrics/analysis/__init__.py b/src/microscopemetrics/analysis/__init__.py deleted file mode 100644 index ce620fa..0000000 --- a/src/microscopemetrics/analysis/__init__.py +++ /dev/null @@ -1,402 +0,0 @@ -import logging -from datetime import datetime -from itertools import product - -import numpy as np - -from microscopemetrics.devices import devices - -# import inspect -# from os import path -# import importlib -# importlib.resources.contents('metrics.samples') -# importlib.import_module('.argolight', package='metrics.samples') - -# import dataset analysis -# from microscopemetrics.samples.dataset import DatasetConfigurator - - -# import samples -# from microscopemetrics.samples.argolight import ArgolightConfigurator -# from microscopemetrics.samples.psf_beads import PSFBeadsConfigurator - -# SAMPLE_CONFIGURATORS = [ArgolightConfigurator, -# PSFBeadsConfigurator] -# noinspection PyUnresolvedReferences -# SAMPLE_HANDLERS = [c.SAMPLE_CLASS for c in SAMPLE_CONFIGURATORS] -# SAMPLE_SECTIONS = [c.CONFIG_SECTION for c in SAMPLE_CONFIGURATORS] -# SAMPLE_ANALYSES = [c.ANALYSES for c in SAMPLE_CONFIGURATORS] - -# Creating logging services -module_logger = logging.getLogger("metrics.analysis") - -# Namespace constants -NAMESPACE_PREFIX = "metrics" -NAMESPACE_ANALYZED = "analyzed" -NAMESPACE_VALIDATED = "validated" -# TODO: Add a special case editable - - -def get_image_data(image, device): - image_name = image.getName() - image_id = image.getId() - raw_img = interface.get_intensities(image) - # Images from Interface come in zctyx dimensions and locally as zcxy. - # The easiest for the moment is to remove t - if raw_img.shape[2] == 1: - raw_img = np.squeeze(raw_img, 2) # TODO: Fix this time dimension. - else: - raise Exception("Image has a time dimension. Time is not yet implemented for this analysis") - pixel_size = interface.get_pixel_size(image) - pixel_size_units = interface.get_pixel_size_units(image) - - device_settings = device.get_all_settings(image=image) - - image_settings = { - "image_data": raw_img, - "image_name": image_name, - "image_id": image_id, - "pixel_size": pixel_size, - "pixel_size_units": pixel_size_units, - } - image_settings.update(device_settings) - - return image_settings - - -def save_data_table( - conn, table_name, col_names, col_descriptions, col_data, interface_obj, namespace -): - table_ann = interface.create_annotation_table( - connection=conn, - table_name=table_name, - column_names=col_names, - column_descriptions=col_descriptions, - values=col_data, - namespace=namespace, - ) - - interface_obj.linkAnnotation(table_ann) - - -def save_data_key_values(conn, key_values, interface_obj, namespace, editable=False): - map_ann = interface.create_annotation_map( - connection=conn, annotation=key_values, namespace=namespace - ) - interface_obj.linkAnnotation(map_ann) - - -def create_roi(conn, shapes, image, name, description): - type_to_func = { - "point": interface.create_shape_point, - "line": interface.create_shape_line, - "rectangle": interface.create_shape_rectangle, - "ellipse": interface.create_shape_ellipse, - "polygon": interface.create_shape_polygon, - "mask": interface.create_shape_mask, - } - - new_shapes = [type_to_func[shape["type"]](**shape["args"]) for shape in shapes] - interface.create_roi( - connection=conn, - image=image, - shapes=new_shapes, - name=name, - description=description, - ) - - -def create_image( - conn, - image_intensities, - image_name, - description, - dataset, - source_image_id=None, - metrics_generated_tag_id=None, -): - zct_list = list( - product( - range(image_intensities.shape[0]), - range(image_intensities.shape[1]), - range(image_intensities.shape[2]), - ) - ) - zct_generator = (image_intensities[z, c, t, :, :] for z, c, t in zct_list) - - new_image = conn.createImageFromNumpySeq( - zctPlanes=zct_generator, - imageName=image_name, - sizeZ=image_intensities.shape[0], - sizeC=image_intensities.shape[1], - sizeT=image_intensities.shape[2], - description=description, - dataset=dataset, - sourceImageId=source_image_id, - ) - - # TODO: see with ome why description is not applied - img_wrapper = conn.getObject("Image", new_image.getId()) - img_wrapper.setDescription(description) - img_wrapper.save() - - if metrics_generated_tag_id is not None: - tag = conn.getObject("Annotation", metrics_generated_tag_id) - if tag is None: - module_logger.warning( - "Metrics tag is not found. New images will not be tagged. Verify metrics tag existence and id." - ) - else: - new_image.linkAnnotation(tag) - - return new_image - - -def analyze_dataset(connection, script_params, dataset, analysis_config, device_config): - # TODO: must note in mapann the analyses that were done - - # TODO: do something to automate selection of microscope type - device = devices.WideFieldMicroscope(device_config) - - module_logger.info(f"Analyzing data from Dataset: {dataset.getId()}") - module_logger.info(f"Date and time: {datetime.now()}") - - # This dictionary keeps track if the hard or soft limits are passed or not for the whole dataset - dataset_limits_passed = { - "uhl_passed": True, - "lhl_passed": True, - "usl_passed": True, - "lsl_passed": True, - "limits": list(), - "sources": list(), - } - - for section, analyses, handler in zip(SAMPLE_SECTIONS, SAMPLE_ANALYSES, SAMPLE_HANDLERS): - if analysis_config.has_section(section): - module_logger.info(f"Running analysis on {section.capitalize()} sample(s)") - section_conf = analysis_config[section] - handler_instance = handler(config=section_conf) - for analysis in analyses: - if section_conf.getboolean(f"analyze_{analysis}"): - namespace = ( - f"{NAMESPACE_PREFIX}/" - f"{NAMESPACE_ANALYZED}/" - f"{handler.get_module()}/" - f"{analysis}/" - f'{analysis_config["MAIN"]["metrics_version"]}' - ) - images = interface.get_tagged_images_in_dataset( - dataset, section_conf.getint(f"{analysis}_image_tag_id") - ) - for image in images: - image_data = get_image_data(image=image, device=device) - ( - out_images, - out_rois, - out_tags, - out_dicts, - out_tables, - image_limits_passed, - ) = handler_instance.analyze_image( - image=image_data, analyses=analysis, config=section_conf - ) - - for out_image in out_images: - create_image( - conn=connection, - image_intensities=out_image["image_data"], - image_name=out_image["image_name"], - description=f'Source Image Id:{image.getId()}\n{out_image["image_desc"]}', - dataset=dataset, - source_image_id=image.getId(), - metrics_generated_tag_id=analysis_config["MAIN"].getint( - "metrics_generated_tag_id" - ), - ) - - for out_roi in out_rois: - create_roi( - conn=connection, - shapes=out_roi["shapes"], - image=image, - name=out_roi["name"], - description=out_roi["desc"], - ) - - for out_tag in out_tags: - pass # TODO implement interface to save tags - - for out_table_name, out_table in out_tables.items(): - save_data_table( - conn=connection, - table_name=out_table_name, - col_names=[p["name"] for p in out_table], - col_descriptions=[p["desc"] for p in out_table], - col_data=[p["data"] for p in out_table], - interface_obj=image, - namespace=namespace, - ) - - for out_dict in out_dicts: - labeled_out_dict = { - "analysis_date_time": f"{datetime.now()}", - "sample_type": f"{handler.get_module()}", - "analysis_type": f"{analysis}", - } - labeled_out_dict.update(out_dict) - - save_data_key_values( - conn=connection, - key_values=labeled_out_dict, - interface_obj=image, - namespace=namespace, - ) - - for ilp in image_limits_passed: - for k, v in ilp.items(): - if v is False: - dataset_limits_passed[k] = False - elif type(v) is list: - dataset_limits_passed[k].extend(v) - - dataset_section = DatasetConfigurator.CONFIG_SECTION - dataset_analyses = DatasetConfigurator.ANALYSES - dataset_handler = DatasetConfigurator.SAMPLE_CLASS - - if analysis_config.has_section(dataset_section): - module_logger.info(f"Running analysis on dataset") - ds_conf = analysis_config[dataset_section] - ds_handler_instance = dataset_handler(config=ds_conf) - for dataset_analysis in dataset_analyses: - if ds_conf.getboolean(f"analyze_{dataset_analysis}"): - namespace = ( - f"{NAMESPACE_PREFIX}/" - f"{NAMESPACE_ANALYZED}/" - f"{dataset_handler.get_module()}/" - f"{dataset_analysis}/" - f'{analysis_config["MAIN"]["metrics_version"]}' - ) - ( - out_images, - out_tags, - out_dicts, - out_editables, - out_tables, - image_limits_passed, - ) = ds_handler_instance.analyze_dataset( - dataset=dataset, analyses=dataset_analysis, config=ds_conf - ) - for out_image in out_images: - create_image( - conn=connection, - image_intensities=out_image["image_data"], - image_name=out_image["image_name"], - description=out_image["image_desc"], - dataset=dataset, - metrics_generated_tag_id=analysis_config["MAIN"].getint( - "metrics_generated_tag_id" - ), - ) - - for out_tag in out_tags: - pass # TODO implement interface to save tags - - for out_table_name, out_table in out_tables.items(): - save_data_table( - conn=connection, - table_name=out_table_name, - col_names=[p["name"] for p in out_table], - col_descriptions=[p["desc"] for p in out_table], - col_data=[p["data"] for p in out_table], - interface_obj=dataset, - namespace=namespace, - ) - - for out_dict, out_editable in zip(out_dicts, out_editables): - if out_editable: - tmp_namespace = None - else: - tmp_namespace = namespace - save_data_key_values( - conn=connection, - key_values=out_dict, - interface_obj=dataset, - namespace=tmp_namespace, - ) - - for ilp in image_limits_passed: - for k, v in ilp.items(): - if v is False: - dataset_limits_passed[k] = False - elif type(v) is list: - dataset_limits_passed[k].extend(v) - - # Save final dataset limits passed tests and corresponding tags - namespace = ( - f"{NAMESPACE_PREFIX}/" - f"{NAMESPACE_ANALYZED}/" - "dataset/" - "limits_verification/" - f'{analysis_config["MAIN"]["metrics_version"]}' - ) - - # dataset_limits_passed['limits'] = list(dict.fromkeys(dataset_limits_passed['limits'])) # Remove duplicates - - if ( - dataset_limits_passed["uhl_passed"] and dataset_limits_passed["lhl_passed"] - ): # Hard limits passed - interface.link_annotation_tag( - connection, - dataset, - analysis_config["MAIN"].getint("passed_hard_limits_tag_id"), - ) - else: - interface.link_annotation_tag( - connection, - dataset, - analysis_config["MAIN"].getint("not_passed_hard_limits_tag_id"), - ) - - if ( - dataset_limits_passed["usl_passed"] and dataset_limits_passed["lsl_passed"] - ): # Soft limits passed - interface.link_annotation_tag( - connection, - dataset, - analysis_config["MAIN"].getint("passed_soft_limits_tag_id"), - ) - else: - interface.link_annotation_tag( - connection, - dataset, - analysis_config["MAIN"].getint("not_passed_soft_limits_tag_id"), - ) - - save_data_key_values( - conn=connection, - key_values=dataset_limits_passed, - interface_obj=dataset, - namespace=namespace, - ) - - try: - if "Comment" in script_params.keys() and script_params["Comment"] != "": - module_logger.info("Adding comment to Dataset.") - namespace = ( - f"{NAMESPACE_PREFIX}/" - f"{NAMESPACE_ANALYZED}/" - "comment/" - "comment/" - f'{analysis_config["MAIN"]["config_version"]}' - ) - - comment_annotation = interface.create_annotation_comment( - connection=connection, - comment_string=script_params["Comment"], - namespace=namespace, - ) - interface.link_annotation(dataset, comment_annotation) - except KeyError: - module_logger.info("No comments added") - - module_logger.info(f"Sample finished for dataset: {dataset.getId()}") diff --git a/src/microscopemetrics/devices/devices.py b/src/microscopemetrics/devices/devices.py index 272ee0a..07d9dc0 100644 --- a/src/microscopemetrics/devices/devices.py +++ b/src/microscopemetrics/devices/devices.py @@ -533,9 +533,7 @@ def get_theoretical_res_rayleigh(self, **kwargs): ) theoretical_res["resolution_theoretical_rayleigh_lateral"].append(None) try: - theoretical_res["resolution_theoretical_rayleigh_axial"].append( - 2 * em * ri / na**2 - ) + theoretical_res["resolution_theoretical_rayleigh_axial"].append(2 * em * ri / na**2) except TypeError as e: module_logger.warning( "Rayleigh theoretical axial resolution could not be calculated. Verify configuration files." diff --git a/src/microscopemetrics/samples/dataset.py b/src/microscopemetrics/samples/dataset.py deleted file mode 100644 index a737d17..0000000 --- a/src/microscopemetrics/samples/dataset.py +++ /dev/null @@ -1,74 +0,0 @@ -import logging -from datetime import datetime - -from microscopemetrics.samples.samples import Analysis, Configurator - -module_logger = logging.getLogger("metrics.samples.dataset") - - -class DatasetConfigurator(Configurator): - """This class handles the configuration properties of the excitation_power sample - - Defines configuration properties - - Helps in the generation of analysis_config files""" - - CONFIG_SECTION = "DATASET" - ANALYSES = ["laser_power_measurement"] - - def __init__(self, config): - super().__init__(config) - - -@DatasetConfigurator.register_sample_analysis -class DatasetAnalysis(Analysis): - """This class handles the Excitation_power sample: - - Defines the logic of the associated analyses - - Defines the creation of reports""" - - def __init__(self, config=None): - dataset_analysis_to_func = { - "laser_power_measurement": self.analyze_laser_power_measurement, - } - self.configurator = DatasetConfigurator(config) - super().__init__(config=config, dataset_analysis_to_func=dataset_analysis_to_func) - - @staticmethod - def analyze_laser_power_measurement(dataset, config): - """Opens a Map annotation where the user can enter the power measurements of the Excitation lasers. Those shoudl - be blocked upon validation - - :param dataset: dataset instance - :param config: MetricsConfig instance defining analysis configuration. - Must contain the analysis parameters defined by the configurator - - :returns a list of images - a list of rois - a list of tags - a list of dicts - a dict containing table_names and tables - """ - module_logger.info(f"Generating light sources annotation...") - - wavelengths = config.getlistint("laser_power_measurement_wavelengths", None) - power_units = config.get("laser_power_measurement_units") - measurement_method = config.get("laser_power_measurement_method") - - if wavelengths is None: - module_logger.error( - "Config defined to do excitation power measurements but No wavelengths were provided" - ) - wavelengths = list() - - key_values = {"analysis_date": datetime.now().strftime("%Y-%m-%d")} - for wave in wavelengths: - key_values.update({str(wave): ""}) - key_values["power_units"] = power_units - key_values["measurement_method"] = measurement_method - - out_images = [] - out_rois = [] - out_tags = [] - out_dicts = [key_values] - out_editables = [True] - out_tables = [] - - return out_images, out_rois, out_tags, out_dicts, out_editables, out_tables diff --git a/src/microscopemetrics/strategies/strategies.py b/src/microscopemetrics/strategies/strategies.py index 74fefc5..951982a 100644 --- a/src/microscopemetrics/strategies/strategies.py +++ b/src/microscopemetrics/strategies/strategies.py @@ -11,15 +11,12 @@ raise ImportError( "In order to run the strategies you need to install the test extras. Run `pip install microscopemetrics[test]`." ) -from microscopemetrics_schema import datamodel as mm_schema from microscopemetrics_schema import strategies as st_mm_schema from skimage.exposure import rescale_intensity as skimage_rescale_intensity from skimage.filters import gaussian as skimage_gaussian from skimage.util import random_noise as skimage_random_noise -from microscopemetrics import samples as mm_samples -from microscopemetrics.samples import ( - argolight, +from microscopemetrics.analyses import ( # argolight, field_illumination, numpy_to_mm_image, psf_beads, @@ -208,7 +205,7 @@ def st_field_illumination_dataset( test_data = draw(test_data) field_illumination_unprocessed_dataset = draw(unprocessed_dataset) - field_illumination_unprocessed_dataset.input.field_illumination_image = [ + field_illumination_unprocessed_dataset.input_data.field_illumination_image = [ numpy_to_mm_image( array=image, name=f"FI_image_{i}", @@ -220,17 +217,21 @@ def st_field_illumination_dataset( # Setting the bit depth to the data type of the image image_dtype = { a.array_data.dtype - for a in field_illumination_unprocessed_dataset.input.field_illumination_image + for a in field_illumination_unprocessed_dataset.input_data.field_illumination_image } if len(image_dtype) != 1: raise ValueError("All images should have the same data type") image_dtype = image_dtype.pop() if np.issubdtype(image_dtype, np.integer): - field_illumination_unprocessed_dataset.input.bit_depth = np.iinfo(image_dtype).bits + field_illumination_unprocessed_dataset.input_parameters.bit_depth = np.iinfo( + image_dtype + ).bits elif np.issubdtype(image_dtype, np.floating): - field_illumination_unprocessed_dataset.input.bit_depth = np.finfo(image_dtype).bits + field_illumination_unprocessed_dataset.input_parameters.bit_depth = np.finfo( + image_dtype + ).bits else: - field_illumination_unprocessed_dataset.input.bit_depth = None + field_illumination_unprocessed_dataset.input_parameters.bit_depth = None return { "unprocessed_dataset": field_illumination_unprocessed_dataset, @@ -254,9 +255,7 @@ def st_field_illumination_table( dataset = draw(st_field_illumination_dataset())["unprocessed_dataset"] dataset.run() if dataset.processed: - key_values = {} - for col in columns: - key_values[col] = getattr(dataset.output.key_values, col) + key_values = {col: getattr(dataset.output.key_values, col) for col in columns} else: continue table.append(key_values) @@ -336,14 +335,14 @@ def _gen_psf_beads_image( z_pos = z_image_shape // 2 y_pos = random.choice( [ - random.randint(5, int(min_distance_y / 2) - 2), - random.randint(y_image_shape - int(min_distance_y / 2) + 2, y_image_shape - 5), + random.randint(5, min_distance_y // 2 - 2), + random.randint(y_image_shape - min_distance_y // 2 + 2, y_image_shape - 5), ] ) x_pos = random.choice( [ - random.randint(5, int(min_distance_x / 2) - 2), - random.randint(x_image_shape - int(min_distance_x / 2) + 2, x_image_shape - 5), + random.randint(5, min_distance_x // 2 - 2), + random.randint(x_image_shape - min_distance_x // 2 + 2, x_image_shape - 5), ] ) if not edge_bead_positions: @@ -480,7 +479,7 @@ def st_psf_beads_test_data( min_distance_y = draw(min_distance) min_distance_x = draw(min_distance) - for image_nr in range(draw(nr_images)): + for _ in range(draw(nr_images)): _nr_valid_beads = draw(nr_valid_beads) _nr_edge_beads = draw(nr_edge_beads) _nr_out_of_focus_beads = draw(nr_out_of_focus_beads) @@ -557,7 +556,7 @@ def st_psf_beads_dataset( test_data = draw(test_data) psf_beads_unprocessed_dataset = draw(unprocessed_dataset) - psf_beads_unprocessed_dataset.input.psf_beads_images = [ + psf_beads_unprocessed_dataset.input_data.psf_beads_images = [ numpy_to_mm_image( array=image, name=f"PSF_image_{i}", @@ -567,16 +566,18 @@ def st_psf_beads_dataset( ] # Setting the bit depth to the data type of the image - image_dtype = {a.array_data.dtype for a in psf_beads_unprocessed_dataset.input.psf_beads_images} + image_dtype = { + a.array_data.dtype for a in psf_beads_unprocessed_dataset.input_data.psf_beads_images + } if len(image_dtype) != 1: raise ValueError("All images should have the same data type") image_dtype = image_dtype.pop() if np.issubdtype(image_dtype, np.integer): - psf_beads_unprocessed_dataset.input.bit_depth = np.iinfo(image_dtype).bits + psf_beads_unprocessed_dataset.input_parameters.bit_depth = np.iinfo(image_dtype).bits elif np.issubdtype(image_dtype, np.floating): - psf_beads_unprocessed_dataset.input.bit_depth = np.finfo(image_dtype).bits + psf_beads_unprocessed_dataset.input_parameters.bit_depth = np.finfo(image_dtype).bits else: - psf_beads_unprocessed_dataset.input.bit_depth = None + psf_beads_unprocessed_dataset.input_parameters.bit_depth = None return { "unprocessed_dataset": psf_beads_unprocessed_dataset, diff --git a/src/microscopemetrics/utilities/__init__.py b/src/microscopemetrics/utilities/__init__.py deleted file mode 100644 index e69de29..0000000 diff --git a/src/microscopemetrics/utilities/utilities.py b/src/microscopemetrics/utilities/utilities.py deleted file mode 100644 index fb0cf44..0000000 --- a/src/microscopemetrics/utilities/utilities.py +++ /dev/null @@ -1,284 +0,0 @@ -# This is a place to hold mere utilities for metrics - -import json -import warnings -from configparser import ConfigParser -from typing import Optional - -import numpy as np -from numpy import float64, ndarray -from scipy import special -from scipy.optimize import curve_fit, fsolve - -INT_DETECTOR_BIT_DEPTHS = [8, 10, 11, 12, 15, 16, 32] -FLOAT_DETECTOR_BIT_DEPTHS = [32, 64] - - -## Some useful functions -def convert_SI(val, unit_in, unit_out): - si = { - "nanometer": 0.000000001, - "micrometer": 0.000001, - "millimeter": 0.001, - "meter": 1.0, - } - return val * si[unit_in.lower()] / si[unit_out.lower()] - - -# def airy_fun(x, centre, a, exp): # , amp, bg): -# if (x - centre) == 0: -# return a * .5 ** exp -# else: -# return a * (special.j1(x - centre) / (x - centre)) ** exp -# -# -# def multi_airy_fun(x, *params): -# y = np.zeros_like(x) -# for i in range(0, len(params), 3): -# y = y + airy_fun(x, params[i], params[i+1], params[i+2]) -# return y -def airy_fun(x: ndarray, centre: float64, amp: float64) -> ndarray: # , exp): # , amp, bg): - with np.errstate(divide="ignore", invalid="ignore"): - return np.where( - (x - centre) == 0, - amp * 0.5**2, - amp * (special.j1(x - centre) / (x - centre)) ** 2, - ) - - -def gaussian_fun(x, background, amplitude, center, sd): - gauss = np.exp(-np.power(x - center, 2.0) / (2 * np.power(sd, 2.0))) - return background + (amplitude - background) * gauss - - -def fit_gaussian(profile, guess=None): - if guess is None: - guess = [profile.min(), profile.max(), profile.argmax(), 0.8] - x = np.linspace(0, profile.shape[0], profile.shape[0], endpoint=False) - popt, pcov, infodict, mesg, ier = curve_fit( - f=gaussian_fun, xdata=x, ydata=profile, p0=guess, maxfev=5000, full_output=True - ) - - if ier not in [1, 2, 3, 4]: - warnings.warn(f"No gaussian fit found. Reason: {mesg}") - - fitted_profile = gaussian_fun(x, popt[0], popt[1], popt[2], popt[3]) - fwhm = popt[3] * 2.35482 - - # Calculate the fit quality using the coefficient of determination (R^2) - y_mean = np.mean(profile) - sst = np.sum((profile - y_mean) ** 2) - ssr = np.sum((profile - fitted_profile) ** 2) - cd_r2 = 1 - (ssr / sst) - - return fitted_profile, cd_r2, fwhm, popt - - -def fit_airy(profile, guess=None): - profile = (profile - profile.min()) / (profile.max() - profile.min()) - if guess is None: - guess = [profile.argmax(), 4 * profile.max()] - x = np.linspace(0, profile.shape[0], profile.shape[0], endpoint=False) - popt, pcov, infodict, mesg, ier = curve_fit( - f=airy_fun, xdata=x, ydata=profile, p0=guess, full_output=True - ) - - if ier not in [1, 2, 3, 4]: - warnings.warn(f"No airy fit found. Reason: {mesg}") - - fitted_profile = airy_fun(x, popt[0], popt[1]) - - # Calculate the FWHM - def _f(d): - return airy_fun(d, popt[0], popt[1]) - (fitted_profile.max() - fitted_profile.min()) / 2 - - guess = np.array([fitted_profile.argmax() - 1, fitted_profile.argmax() + 1]) - v = fsolve(_f, guess) - fwhm = abs(v[1] - v[0]) - - # Calculate the fit quality using the coefficient of determination (R^2) - y_mean = np.mean(profile) - sst = np.sum((profile - y_mean) ** 2) - ssr = np.sum((profile - fitted_profile) ** 2) - cd_r2 = 1 - (ssr / sst) - - return fitted_profile, cd_r2, fwhm, popt - - -def multi_airy_fun(x: ndarray, *params) -> ndarray: - y = np.zeros_like(x) - for i in range(0, len(params), 2): - y = y + airy_fun(x, params[i], params[i + 1]) - return y - - -def robust_z_score(data): - median = np.median(data) - mad = np.median(np.abs(data - median)) - return 0.6745 * (data - median) / mad - - -def z_score(data): - mean = np.mean(data) - std_dev = np.std(data) - return (data - mean) / std_dev - - -def wavelength_to_rgb(wavelength, gamma=0.8): - """ - Copied from https://www.noah.org/wiki/Wavelength_to_RGB_in_Python - This converts a given wavelength of light to an - approximate RGB color value. The wavelength must be given - in nanometers in the range from 380 nm through 750 nm - (789 THz through 400 THz). - - Based on code by Dan Bruton - http://www.physics.sfasu.edu/astro/color/spectra.html - """ - - wavelength = float(wavelength) - if 380 < wavelength <= 440: - attenuation = 0.3 + 0.7 * (wavelength - 380) / (440 - 380) - r = ((-(wavelength - 440) / (440 - 380)) * attenuation) ** gamma - g = 0.0 - b = (1.0 * attenuation) ** gamma - elif 440 < wavelength <= 490: - r = 0.0 - g = ((wavelength - 440) / (490 - 440)) ** gamma - b = 1.0 - elif 490 < wavelength <= 510: - r = 0.0 - g = 1.0 - b = (-(wavelength - 510) / (510 - 490)) ** gamma - elif 510 < wavelength <= 580: - r = ((wavelength - 510) / (580 - 510)) ** gamma - g = 1.0 - b = 0.0 - elif 580 < wavelength <= 645: - r = 1.0 - g = (-(wavelength - 645) / (645 - 580)) ** gamma - b = 0.0 - elif 645 < wavelength <= 750: - attenuation = 0.3 + 0.7 * (750 - wavelength) / (750 - 645) - r = (1.0 * attenuation) ** gamma - g = 0.0 - b = 0.0 - else: - r = 0.0 - g = 0.0 - b = 0.0 - r *= int(r * 255) - g *= int(g * 255) - g *= int(g * 255) - return r, g, b - - -# This class is not used after introduction of pydantic. -class MetricsConfig(ConfigParser): - def getjson(self, section, option, **kwargs): - value = self.get(section, option, **kwargs) - try: - output = json.loads(value) - except json.JSONDecodeError as e: - raise e - return output - - def getlist(self, section, option, **kwargs): - output = self.getjson(section, option, **kwargs) - - if type(output) is list: - return output - else: - raise Exception( - f'The config option "{option}" in section "{section}" is not formatted as a list' - ) - - def getlistint(self, section, option, **kwargs): - try: - output = [int(x) for x in self.getlist(section, option, **kwargs)] - return output - except Exception as e: - print( - f'Some element in config option "{option}" in section "{section}" cannot be coerced into a integer' - ) - raise e - - def getlistfloat(self, section, option, **kwargs): - try: - output = [float(x) for x in self.getlist(section, option, **kwargs)] - return output - except Exception as e: - print( - f'Some element in config option "{option}" in section "{section}" cannot be coerced into a float' - ) - raise e - - -def is_saturated( - channel: ndarray, threshold: float = 0.0, detector_bit_depth: Optional[int] = None -) -> bool: - """ - Checks if the channel is saturated. - A warning if it suspects that the detector bit depth does not match the datatype. - thresh: float - Threshold for the ratio of saturated pixels to total pixels - detector_bit_depth: int - Bit depth of the detector. Sometimes, detectors bit depth are not matching the datatype of the measureemnts. - Here it can be specified the bit depth of the detector if known. The function is going to raise - If None, it will be inferred from the channel dtype. - """ - if detector_bit_depth is not None: - if ( - detector_bit_depth not in INT_DETECTOR_BIT_DEPTHS - and detector_bit_depth not in FLOAT_DETECTOR_BIT_DEPTHS - ): - raise ValueError( - f"The detector bit depth provided ({detector_bit_depth}) is not supported. Supported values are {INT_DETECTOR_BIT_DEPTHS} for integer detectors and {FLOAT_DETECTOR_BIT_DEPTHS} for floating point detectors." - ) - if ( - np.issubdtype(channel.dtype, np.integer) - and detector_bit_depth not in INT_DETECTOR_BIT_DEPTHS - ): - raise ValueError( - f"The channel datatype {channel.dtype} does not match the detector bit depth {detector_bit_depth}. The channel might be saturated." - ) - elif ( - np.issubdtype(channel.dtype, np.floating) - and detector_bit_depth not in FLOAT_DETECTOR_BIT_DEPTHS - ): - raise ValueError( - f"The channel datatype {channel.dtype} does not match the detector bit depth {detector_bit_depth}. The channel might be saturated." - ) - else: - if np.issubdtype(channel.dtype, np.integer): - if detector_bit_depth > np.iinfo(channel.dtype).bits: - raise ValueError( - f"The channel datatype {channel.dtype} does not support the detector bit depth {detector_bit_depth}. The channel might be saturated." - ) - else: - max_limit = pow(2, detector_bit_depth) - 1 - elif np.issubdtype(channel.dtype, np.floating): - if detector_bit_depth != np.finfo(channel.dtype).bits: - raise ValueError( - f"The channel datatype {channel.dtype} does not support the detector bit depth {detector_bit_depth}. The channel might be saturated." - ) - else: - max_limit = np.finfo(channel.dtype).max - - else: - if np.issubdtype(channel.dtype, np.integer): - max_limit = np.iinfo(channel.dtype).max - elif np.issubdtype(channel.dtype, np.floating): - max_limit = np.finfo(channel.dtype).max - else: - raise ValueError("The channel provided is not a valid numpy dtype.") - - if channel.max() > max_limit: - raise ValueError( - "The channel provided has values larger than the bit depth of the detector." - ) - - saturation_matrix = channel == max_limit - saturation_ratio = np.count_nonzero(saturation_matrix) / channel.size - - return saturation_ratio > threshold diff --git a/tests/unit/samples/test_argolight.py b/tests/unit/analyses/test_argolight.py similarity index 97% rename from tests/unit/samples/test_argolight.py rename to tests/unit/analyses/test_argolight.py index 6bf66bc..eaa2ea4 100644 --- a/tests/unit/samples/test_argolight.py +++ b/tests/unit/analyses/test_argolight.py @@ -1,7 +1,7 @@ import numpy as np import pytest -from microscopemetrics.samples import argolight, numpy_to_mm_image +# from microscopemetrics.analyses import argolight, numpy_to_mm_image from tests.test_utilities import get_file diff --git a/tests/unit/samples/test_field_illumination.py b/tests/unit/analyses/test_field_illumination.py similarity index 83% rename from tests/unit/samples/test_field_illumination.py rename to tests/unit/analyses/test_field_illumination.py index ed2786a..d300021 100644 --- a/tests/unit/samples/test_field_illumination.py +++ b/tests/unit/analyses/test_field_illumination.py @@ -4,7 +4,7 @@ from microscopemetrics_schema import datamodel as mm_schema from microscopemetrics import SaturationError -from microscopemetrics.samples import field_illumination +from microscopemetrics.analyses import field_illumination from microscopemetrics.strategies import strategies as st_mm @@ -16,7 +16,7 @@ def test_field_illumination_analysis_instantiation(dataset): assert dataset.name assert dataset.description assert dataset.microscope - assert dataset.input + assert dataset.input_parameters @given(st_mm.st_field_illumination_dataset()) @@ -24,7 +24,7 @@ def test_field_illumination_analysis_instantiation(dataset): def test_field_illumination_analysis_run(dataset): dataset = dataset["unprocessed_dataset"] assert not dataset.processed - assert field_illumination.analise_field_illumination(dataset) + assert field_illumination.analyse_field_illumination(dataset) assert dataset.processed @@ -39,7 +39,7 @@ def test_field_illumination_analysis_run(dataset): def test_field_illumination_analysis_centers_geometric(dataset): field_illumination_dataset = dataset["unprocessed_dataset"] expected_output = dataset["expected_output"] - field_illumination.analise_field_illumination(field_illumination_dataset) + field_illumination.analyse_field_illumination(field_illumination_dataset) measured_centers = list( zip( @@ -56,9 +56,9 @@ def test_field_illumination_analysis_centers_geometric(dataset): note(f"Expected output: {expected_output}") note( f"Input params: " - f"bit_depth: {field_illumination_dataset.input.bit_depth}" - f"saturation_threshold: {field_illumination_dataset.input.saturation_threshold}" - f"sigma: {field_illumination_dataset.input.sigma}" + f"bit_depth: {field_illumination_dataset.input_parameters.bit_depth}" + f"saturation_threshold: {field_illumination_dataset.input_parameters.saturation_threshold}" + f"sigma: {field_illumination_dataset.input_parameters.sigma}" ) for measured_c, expected_c in zip(measured_centers, expected_centers): @@ -77,7 +77,7 @@ def test_field_illumination_analysis_centers_geometric(dataset): def test_field_illumination_analysis_centers_of_mass(dataset): field_illumination_dataset = dataset["unprocessed_dataset"] expected_output = dataset["expected_output"] - field_illumination.analise_field_illumination(field_illumination_dataset) + field_illumination.analyse_field_illumination(field_illumination_dataset) measured_centers_weighted = list( zip( @@ -94,9 +94,9 @@ def test_field_illumination_analysis_centers_of_mass(dataset): note(f"Expected output: {expected_output}") note( f"Input params: " - f"bit_depth: {field_illumination_dataset.input.bit_depth}" - f"saturation_threshold: {field_illumination_dataset.input.saturation_threshold}" - f"sigma: {field_illumination_dataset.input.sigma}" + f"bit_depth: {field_illumination_dataset.input_parameters.bit_depth}" + f"saturation_threshold: {field_illumination_dataset.input_parameters.saturation_threshold}" + f"sigma: {field_illumination_dataset.input_parameters.sigma}" ) for measured_c_w, expected_c in zip(measured_centers_weighted, expected_centers): @@ -108,7 +108,7 @@ def test_field_illumination_analysis_centers_of_mass(dataset): def test_field_illumination_analysis_max_intensity_positions(dataset): field_illumination_dataset = dataset["unprocessed_dataset"] expected_output = dataset["expected_output"] - field_illumination.analise_field_illumination(field_illumination_dataset) + field_illumination.analyse_field_illumination(field_illumination_dataset) measured_max_intensity_positions = list( zip( @@ -125,9 +125,9 @@ def test_field_illumination_analysis_max_intensity_positions(dataset): note(f"Expected output: {expected_output}") note( f"Input params: " - f"bit_depth: {field_illumination_dataset.input.bit_depth}" - f"saturation_threshold: {field_illumination_dataset.input.saturation_threshold}" - f"sigma: {field_illumination_dataset.input.sigma}" + f"bit_depth: {field_illumination_dataset.input_parameters.bit_depth}" + f"saturation_threshold: {field_illumination_dataset.input_parameters.saturation_threshold}" + f"sigma: {field_illumination_dataset.input_parameters.sigma}" ) for measured_m_i, expected_c in zip(measured_max_intensity_positions, expected_centers): @@ -139,7 +139,7 @@ def test_field_illumination_analysis_max_intensity_positions(dataset): def test_field_illumination_analysis_centers_fitted(dataset): field_illumination_dataset = dataset["unprocessed_dataset"] expected_output = dataset["expected_output"] - field_illumination.analise_field_illumination(field_illumination_dataset) + field_illumination.analyse_field_illumination(field_illumination_dataset) measured_centers_fitted = list( zip( @@ -156,9 +156,9 @@ def test_field_illumination_analysis_centers_fitted(dataset): note(f"Expected output: {expected_output}") note( f"Input params: " - f"bit_depth: {field_illumination_dataset.input.bit_depth}" - f"saturation_threshold: {field_illumination_dataset.input.saturation_threshold}" - f"sigma: {field_illumination_dataset.input.sigma}" + f"bit_depth: {field_illumination_dataset.input_parameters.bit_depth}" + f"saturation_threshold: {field_illumination_dataset.input_parameters.saturation_threshold}" + f"sigma: {field_illumination_dataset.input_parameters.sigma}" ) for measured_m_i, expected_c in zip(measured_centers_fitted, expected_centers): @@ -176,4 +176,4 @@ def test_field_illumination_analysis_centers_fitted(dataset): def test_field_illumination_analysis_raises_saturation_error(dataset): with pytest.raises(SaturationError): dataset = dataset["unprocessed_dataset"] - field_illumination.analise_field_illumination(dataset) + field_illumination.analyse_field_illumination(dataset) diff --git a/tests/unit/samples/test_psf_beads.py b/tests/unit/analyses/test_psf_beads.py similarity index 94% rename from tests/unit/samples/test_psf_beads.py rename to tests/unit/analyses/test_psf_beads.py index 706ae75..968ab6d 100644 --- a/tests/unit/samples/test_psf_beads.py +++ b/tests/unit/analyses/test_psf_beads.py @@ -9,9 +9,9 @@ from skimage.filters import gaussian from skimage.util import random_noise as skimage_random_noise -from microscopemetrics.samples import psf_beads +from microscopemetrics.analyses import psf_beads from microscopemetrics.strategies import strategies as st_mm -from microscopemetrics.utilities.utilities import fit_gaussian +from microscopemetrics.analyses.tools import fit_gaussian @given( @@ -79,7 +79,7 @@ def test_psf_beads_analysis_instantiation(dataset): assert dataset.name assert dataset.description assert dataset.microscope - assert dataset.input + assert dataset.input_parameters @given(st_mm.st_psf_beads_dataset()) @@ -106,7 +106,7 @@ def test_psf_beads_analysis_run(dataset): ), unprocessed_dataset=st_mm_schema.st_mm_psf_beads_unprocessed_dataset( dataset=st_mm_schema.st_mm_dataset( - input=st_mm_schema.st_mm_psf_beads_input( + input_parameters=st_mm_schema.st_mm_psf_beads_input_parameters( fitting_r2_threshold=st.just(0.8), # TODO: Remove this? intensity_robust_z_score_threshold=st.just(4.0), ) @@ -117,7 +117,9 @@ def test_psf_beads_analysis_run(dataset): def test_psf_beads_analysis_nr_valid_beads(dataset): psf_beads_dataset = dataset["unprocessed_dataset"] expected_output = dataset["expected_output"] - psf_beads_dataset.input.min_lateral_distance_factor = expected_output["min_distance_y"][0] + psf_beads_dataset.input_parameters.min_lateral_distance_factor = expected_output[ + "min_distance_y" + ][0] psf_beads.analyse_psf_beads(psf_beads_dataset) @@ -145,7 +147,9 @@ def test_psf_beads_analysis_nr_lateral_edge_beads(dataset): psf_beads_dataset = dataset["unprocessed_dataset"] expected_output = dataset["expected_output"] psf_beads.analyse_psf_beads(psf_beads_dataset) - psf_beads_dataset.input.min_lateral_distance_factor = expected_output["min_distance_y"][0] + psf_beads_dataset.input_parameters.min_lateral_distance_factor = expected_output[ + "min_distance_y" + ][0] expected = sum(len(im_ebp) for im_ebp in expected_output["edge_bead_positions"]) diff --git a/tests/unit/test_util_functions.py b/tests/unit/test_analysis_tools.py similarity index 69% rename from tests/unit/test_util_functions.py rename to tests/unit/test_analysis_tools.py index 32e723d..a78698a 100644 --- a/tests/unit/test_util_functions.py +++ b/tests/unit/test_analysis_tools.py @@ -2,7 +2,7 @@ from hypothesis import given from hypothesis import strategies as st -from microscopemetrics.utilities import utilities +from microscopemetrics.analyses.tools import is_saturated @given(st.sampled_from([np.uint8, np.uint16, np.float32])) @@ -17,7 +17,7 @@ def test_is_saturated(data_type): unsaturated_data[0:5, ...] = max_value / 2 # 50% of the image is saturated saturated_data = np.zeros((10, 10), data_type) saturated_data[0:5, ...] = max_value # 50% of the image is saturated - assert not utilities.is_saturated(unsaturated_data) - assert not utilities.is_saturated(unsaturated_data, threshold=0.4) - assert utilities.is_saturated(saturated_data) - assert not utilities.is_saturated(saturated_data, threshold=0.6) + assert not is_saturated(unsaturated_data) + assert not is_saturated(unsaturated_data, threshold=0.4) + assert is_saturated(saturated_data) + assert not is_saturated(saturated_data, threshold=0.6) diff --git a/tox.ini b/tox.ini index 14d5dfb..8c7f544 100644 --- a/tox.ini +++ b/tox.ini @@ -12,4 +12,4 @@ skip_install = true commands = poetry install - pytest + pytest --hypothesis-profile=dev