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Can I use Kourami on other species? #29

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yuanfeng719 opened this issue Aug 11, 2020 · 0 comments
Open

Can I use Kourami on other species? #29

yuanfeng719 opened this issue Aug 11, 2020 · 0 comments

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@yuanfeng719
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Hi,
I am thinking about assembling MHC-I of some other non-human species using Kourami. And I prepared the input bam file and the reference directory following the guideline for Human HLA. Here is an error message I got.

java -jar $script/Kourami.jar -d $ref -o KouramiPred Kourami.bam

----------------REF GRAPH CONSTRUCTION--------------
java.lang.NullPointerException
at MergeMSFs.merge(MergeMSFs.java:264)
at HLA.loadGraphs(HLA.java:82)
at HLA.(HLA.java:62)
at HLA.main(HLA.java:556)

Do you have any idea what might be the reason for the error? And is it doable for other species?

Thanks,
Yuan

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