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<a class="navbar-brand" href="index.html">A Broadly Conserved Deoxycytidine Deaminase Protects Bacteria from Phage Infection</a>
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<h1 class="title toc-ignore">Cite</h1>
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<div id="a-broadly-conserved-deoxycytidine-deaminase-protects-bacteria-from-phage-infection" class="section level2">
<h2><strong>A Broadly Conserved Deoxycytidine Deaminase Protects Bacteria from Phage Infection</strong></h2>
<p><strong>Geoffrey B. Severin<sup>1#@</sup>, Brian Y. Hsueh<sup>2#</sup>, Clinton A. Elg<sup>3</sup>, John A. Dover<sup>1</sup>, Christopher R. Rhoades<sup>2</sup>, Alex J. Wessel<sup>2</sup>, Benjamin J. Ridenhour<sup>4</sup>, Eva M. Top<sup>3</sup>, Janani Ravi<sup>5</sup>, Kristin N. Parent<sup>1</sup>, and Christopher M. Waters<sup>2*</sup></strong></p>
<p><sup>1</sup>Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan, USA, 48824. <sup>2</sup>Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA, 48824. <sup>3</sup>Department of Biological Sciences, Institute for Bioinformatics and Evolutionary Studies, Bioinformatics and Computational Biology Program, University of Idaho, Moscow, Idaho, USA, 83844. <sup>4</sup>Department of Mathematics and Statistical Sciences, University of Idaho, Moscow, Idaho, USA, 83844. <sup>5</sup>Department of Pathobiology and Diagnostic Investigation, Michigan State University, East Lansing, Michigan, USA, 48824.<br />
<sup>@</sup>Current address: Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, 48109.</p>
<p><sup>#</sup>These authors contributed equally to this work.</p>
<p>*<strong>Corresponding Author:</strong> 5180 Biomedical and Physical Sciences, 567 Wilson Road, East Lansing, MI 48824. Telephone: 517-884-5360. E-mail: <a href="mailto:[email protected]">[email protected]</a></p>
<p><strong>Running Title:</strong> <em>A Broadly Conserved Cytidine Deaminase Protects Bacteria from Phage Infection</em></p>
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<h2>Abstract</h2>
<p>The El Tor biotype of <em>Vibrio cholerae</em> is responsible for perpetuating the longest cholera pandemic in recorded history (1961-current). The genomic islands VSP-1 and -2 are two understudied genetic features that distinguish El Tor from previous pandemics. To understand their utility, we calculated the co-occurrence of VSP genes across bacterial genomes. This analysis predicted the previously uncharacterized <em>vc0175</em>, herein renamed <strong>d</strong>eoxycytidylate <strong>d</strong>eaminase <strong>V</strong>ibrio (<em>dcdV</em>), is in a gene network with <em>dncV</em>, a cyclic GMP-AMP synthase involved in phage defense. DcdV consists of two domains, a P-loop kinase and a deoxycytidylate deaminase, that are required for the deamination of dCTP and dCMP, inhibiting phage predation by corrupting cellular nucleotide concentrations. Additionally, DcdV is post-translationally inhibited by a unique noncoding RNA encoded 5’ of the <em>dcdV</em> locus. DcdV homologs are conserved in bacteria and eukaryotes and our results identify <em>V. cholerae</em> DcdV as the founding member of a previously undescribed bacterial phage defense system.</p>
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<h2>Keywords</h2>
<p>cytidine deaminase, phage, genomic island, toxin-antitoxin, thymineless death, APOBEC</p>
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<div id="citation" class="section level2">
<h2>Citation</h2>
<p>A Broadly Conserved Deoxycytidine Deaminase Protects Bacteria from Phage Infection</p>
<p>Geoffrey B. Severin, Brian Y. Hsueh, Clinton A. Elg, John A. Dover, Christopher R.Rhoades, Alex J. Wessel, Benjamin J. Ridenhour, Eva M. Top, Janani Ravi, Kristin N.Parent, Christopher M. Waters.</p>
<p><em>bioRxiv</em> 2021.03.31.437871; doi: <a href="https://doi.org/10.1101/2021.03.31.437871" class="uri">https://doi.org/10.1101/2021.03.31.437871</a></p>
<p><svg aria-hidden="true" role="img" viewBox="0 0 512 512" style="height:1em;width:1em;vertical-align:-0.125em;margin-right:0.2em;font-size:inherit;fill:darkred;overflow:visible;position:relative;"><path d="M448 192V77.25c0-8.49-3.37-16.62-9.37-22.63L393.37 9.37c-6-6-14.14-9.37-22.63-9.37H96C78.33 0 64 14.33 64 32v160c-35.35 0-64 28.65-64 64v112c0 8.84 7.16 16 16 16h48v96c0 17.67 14.33 32 32 32h320c17.67 0 32-14.33 32-32v-96h48c8.84 0 16-7.16 16-16V256c0-35.35-28.65-64-64-64zm-64 256H128v-96h256v96zm0-224H128V64h192v48c0 8.84 7.16 16 16 16h48v96zm48 72c-13.25 0-24-10.75-24-24 0-13.26 10.75-24 24-24s24 10.74 24 24c0 13.25-10.75 24-24 24z"/></svg> <a href="https://www.biorxiv.org/content/10.1101/2021.03.31.437871v1.full.pdf">PDF</a></p>
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<p>Copyright © 2021 Waters Lab and JRaviLab. All rights reserved. </p>
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