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BATCH_AutoBleachCorrect_Personalized
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BATCH_AutoBleachCorrect_Personalized
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/*
* Macro template to process multiple images in a folder
*/
#@ File (label = "Input directory", style = "directory") input
#@ File (label = "Output directory", style = "directory") output
#@ String (label = "File suffix", value = ".tif") suffix
// See also Process_Folder.py for a version of this code
// in the Python scripting language.
processFolder(input);
// function to scan folders/subfolders/files to find files with correct suffix
function processFolder(input) {
list = getFileList(input);
list = Array.sort(list);
for (i = 0; i < list.length; i++) {
if(File.isDirectory(input + File.separator + list[i]))
processFolder(input + File.separator + list[i]);
if(endsWith(list[i], suffix))
processFile(input, output, list[i]);
}
}
function processFile(input, output, file) {
open(input+File.separator+list[i]);
name = getTitle();
NoExt = File.nameWithoutExtension;
Stack.getDimensions(width, height, channels, slices, frames);
run("Split Channels");
for (i = 1; i <= channels; i++) {
selectImage("C"+i+"-"+name);
chname = getTitle();
run("Bleach Correction", "correction=[Histogram Matching] background=5");
selectImage(chname);
close();
}
run("Merge Channels...", "c1=DUP_C2-"+name+" c2=DUP_C1-"+name+" create ignore");
saveAs('tif', output+File.separator +'DUP_'+name);
close("*");
}