Singularity.def

Bootstrap: docker
From: ubuntu:20.04

%post

	# Set timezone in tzdata
	export DEBIAN_FRONTEND="noninteractive"
	export DEBCONF_NONINTERACTIVE_SEEN=true
	export TZ="Europe/Berlin"
	
	# Install required packages
	apt-get update
	apt-get install -y build-essential wget git autoconf
	
	# Install dependencies for AUGUSTUS comparative gene prediction mode (CGP)
	apt-get install -y libgsl-dev libboost-all-dev libsuitesparse-dev liblpsolve55-dev
	apt-get install -y libsqlite3-dev libmysql++-dev
	
	# Install dependencies for the optional support of gzip compressed input files
	apt-get install -y libboost-iostreams-dev zlib1g-dev
	
	# Install dependencies for bam2hints and filterBam
	apt-get install -y libbamtools-dev
	
	# Install additional dependencies for bam2wig
	apt-get install -y samtools libhts-dev
	
	# Install additional dependencies for homGeneMapping and utrrnaseq
	apt-get install -y libboost-all-dev
	
	# Install additional dependencies for scripts
	apt-get install -y cdbfasta diamond-aligner libfile-which-perl libparallel-forkmanager-perl libyaml-perl libdbd-mysql-perl
	apt-get install -y --no-install-recommends python3-biopython
	
	# Install hal - required by homGeneMapping
	# execute the commented out code if you want to use homGeneMapping
#	apt-get install -y libhdf5-dev
#	git clone https://github.com/benedictpaten/sonLib.git /opt/sonLib
#	cd /opt/sonLib
#	make
#	git clone https://github.com/ComparativeGenomicsToolkit/hal.git /opt/hal
#	cd /opt/hal
#	export RANLIB=ranlib
#	make
#%environment
#	export PATH="${PATH}:/opt/hal/bin"

%files
	# Clone AUGUSTUS repository
	. /opt/augustus

%post
	# Build AUGUSTUS
	cd /opt/augustus
	make clean
	make
	make install INSTALLDIR=/opt/augustus

%environment
	export LC_ALL=C
	export PATH="/opt/augustus/bin:/opt/augustus/scripts:${PATH}"

%test
	# Test AUGUSTUS
	cd /opt/augustus
	make unit_test

%help
Create a Singularity Image File from this Singularity Definition File using
	$ git clone https://github.com/Gaius-Augustus/Augustus.git
	$ cd Augustus
	$ singularity build augustus.sif Singularity.def

Running an example (from the Augustus directory) with Singularity:
	$ singularity exec augustus.sif augustus --species=human --UTR=on examples/example.fa