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Introduction to BioImage Analysis

We will use ImageJ, and in particular FIJI to illustrate the most basic concepts of bio-image Analysis

If you want to follow the training material please download and install FIJI following the instructions provided on their website.

Link to external material

Direct link to the presentation

Link to the folder containing also the data

Youtube video for the live demo

Here the link for the recording of the live demo:

Go here for the recording of the live demo

List of resources

Sites

ImageJ/FIJI: https://imagej.net/Learn

NEUBIAS: http://eubias.org/NEUBIAS/

Image.sc Forum https://forum.image.sc/

Bio-Image analysis wiki http://wiki.cmci.info/start

Books:

Bioimage Data Analysis, Editor: Kota Miura

https://analyticalscience.wiley.com/do/10.1002/was.00050003

Bioimage Data Analysis Workflows, Editors: Kota Miura and Nataša Sladoje

https://www.springer.com/gp/book/9783030223854

Program

  • Images are numbers
    • LUTs, and multidimensional data
  • Pre-processing
    • Image filters
  • Segmentation
    • Post processing: morphological operations
  • Quantification
    • Analyze particles, ROI manager