diff --git a/changelog/chore-remove-woopayments-dev-test-mode-calls b/changelog/chore-remove-woopayments-dev-test-mode-calls
new file mode 100644
index 00000000000..0215265352c
--- /dev/null
+++ b/changelog/chore-remove-woopayments-dev-test-mode-calls
@@ -0,0 +1,4 @@
+Significance: minor
+Type: update
+
+chore: remove deprecated is_in_dev_mode() and is_in_test_mode() methods
diff --git a/includes/class-wc-payment-gateway-wcpay.php b/includes/class-wc-payment-gateway-wcpay.php
index 136a8eaa4b6..f795a7514b7 100644
--- a/includes/class-wc-payment-gateway-wcpay.php
+++ b/includes/class-wc-payment-gateway-wcpay.php
@@ -4507,34 +4507,6 @@ public function find_duplicates() {
 		return $this->duplicate_payment_methods_detection_service->find_duplicates();
 	}
 
-	// Start: Deprecated functions.
-
-	/**
-	 * Check the defined constant to determine the current plugin mode.
-	 *
-	 * @deprecated 5.6.0
-	 *
-	 * @return bool
-	 */
-	public function is_in_dev_mode() {
-		wc_deprecated_function( __FUNCTION__, '5.6.0', 'WC_Payments::mode()->is_dev()' );
-		return WC_Payments::mode()->is_dev();
-	}
-
-	/**
-	 * Returns whether test_mode or dev_mode is active for the gateway
-	 *
-	 * @deprecated 5.6.0
-	 *
-	 * @return boolean Test mode enabled if true, disabled if false
-	 */
-	public function is_in_test_mode() {
-		wc_deprecated_function( __FUNCTION__, '5.6.0', 'WC_Payments::mode()->is_test()' );
-		return WC_Payments::mode()->is_test();
-	}
-
-	// End: Deprecated functions.
-
 	/**
 	 * Determine whether redirection is needed for the non-card UPE payment method.
 	 *
diff --git a/tests/unit/test-class-wc-payment-gateway-wcpay.php b/tests/unit/test-class-wc-payment-gateway-wcpay.php
index b97fad22eea..b638c07e6dd 100644
--- a/tests/unit/test-class-wc-payment-gateway-wcpay.php
+++ b/tests/unit/test-class-wc-payment-gateway-wcpay.php
@@ -3444,41 +3444,6 @@ public function is_woopay_falsy_value_provider() {
 		];
 	}
 
-	/**
-	 * @expectedDeprecated is_in_dev_mode
-	 */
-	public function test_is_in_dev_mode() {
-		$mode = WC_Payments::mode();
-
-		$mode->dev();
-		$this->assertTrue( $this->card_gateway->is_in_dev_mode() );
-
-		$mode->live_mode_onboarding();
-		$this->assertFalse( $this->card_gateway->is_in_dev_mode() );
-
-		$mode->live();
-		$this->assertFalse( $this->card_gateway->is_in_dev_mode() );
-	}
-
-	/**
-	 * @expectedDeprecated is_in_test_mode
-	 */
-	public function test_is_in_test_mode() {
-		$mode = WC_Payments::mode();
-
-		$mode->dev();
-		$this->assertTrue( $this->card_gateway->is_in_test_mode() );
-
-		$mode->test_mode_onboarding();
-		$this->assertTrue( $this->card_gateway->is_in_test_mode() );
-
-		$mode->test();
-		$this->assertTrue( $this->card_gateway->is_in_test_mode() );
-
-		$mode->live();
-		$this->assertFalse( $this->card_gateway->is_in_test_mode() );
-	}
-
 	/**
 	 * Create a partial mock for WC_Payment_Gateway_WCPay class.
 	 *