diff --git a/.github/workflows/build-master.yml b/.github/workflows/build-master.yml
index 7400330..938264f 100644
--- a/.github/workflows/build-master.yml
+++ b/.github/workflows/build-master.yml
@@ -15,7 +15,7 @@ jobs:
     runs-on: ${{ matrix.os }}
     strategy:
       matrix:
-        python-version: [3.7, 3.8]
+        python-version: [3.7, 3.8, 3.9]
         os: [ubuntu-latest, windows-latest, macOS-latest]
 
     steps:
@@ -41,7 +41,7 @@ jobs:
     - name: Set up Python
       uses: actions/setup-python@v1
       with:
-        python-version: 3.8
+        python-version: 3.9
     - name: Install Dependencies
       run: |
         python -m pip install --upgrade pip
diff --git a/.github/workflows/publish.yml b/.github/workflows/publish.yml
index 6aaeef8..fe45793 100644
--- a/.github/workflows/publish.yml
+++ b/.github/workflows/publish.yml
@@ -13,7 +13,7 @@ jobs:
     - name: Set up Python
       uses: actions/setup-python@v1
       with:
-        python-version: 3.8
+        python-version: 3.9
     - name: Install Dependencies
       run: |
         python -m pip install --upgrade pip
diff --git a/.github/workflows/test-and-lint.yml b/.github/workflows/test-and-lint.yml
index 6b23208..c45a04d 100644
--- a/.github/workflows/test-and-lint.yml
+++ b/.github/workflows/test-and-lint.yml
@@ -7,7 +7,7 @@ jobs:
     runs-on: ${{ matrix.os }}
     strategy:
       matrix:
-        python-version: [3.7, 3.8]
+        python-version: [3.7, 3.8, 3.9]
         os: [ubuntu-latest, windows-latest, macOS-latest]
 
     steps:
@@ -32,7 +32,7 @@ jobs:
     - name: Set up Python
       uses: actions/setup-python@v1
       with:
-        python-version: 3.8
+        python-version: 3.9
     - name: Install Dependencies
       run: |
         python -m pip install --upgrade pip
diff --git a/setup.py b/setup.py
index 50603ef..85a1912 100644
--- a/setup.py
+++ b/setup.py
@@ -39,7 +39,7 @@
 ]
 
 requirements = [
-    "aicsimageio>=3.2.3",
+    "aicsimageio>=3.3.3",
     # napari plugins is the `napari>=0.3.0` release
     # If someone is trying to use plugins they should have everything they need
     # for this package to work
@@ -53,7 +53,7 @@
     "all": [
         *requirements,
         *dev_requirements,
-    ]
+    ],
 }
 
 setup(
@@ -71,6 +71,7 @@
         "Natural Language :: English",
         "Programming Language :: Python :: 3.7",
         "Programming Language :: Python :: 3.8",
+        "Programming Language :: Python :: 3.9",
     ],
     description="AICSImageIO bindings for napari",
     entry_points={
diff --git a/tox.ini b/tox.ini
index aa33c5c..54e16fc 100644
--- a/tox.ini
+++ b/tox.ini
@@ -1,6 +1,6 @@
 [tox]
 skipsdist = True
-envlist = py37, py38, lint
+envlist = py37, py38, py39, lint
 
 [testenv:lint]
 deps =